Molecular characterization of TRP1 , a gene coding for trytophan synthetase in the basidiomycete Coprinus cinereus

Molecular characterization of TRP1 , a gene coding for trytophan synthetase in the basidiomycete Coprinus cinereus

We utilized a cloned gene (TRP5 ) encoding tryptophan synthetase (TSase) from Saccharomyces cerevisiae to identify and clone the corresponding gene (TRP1 ) from the basidiomycete Coprinus cinereus . The primary nucleotide (nt) sequence of this gene was determined and compared to sequences from other...

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Veröffentlicht in:Gene 1989-01, Vol.81 (1), p.73-82
Hauptverfasser: Skrzynia, C, Binninger, D M, Alspaugh, JA II, Pukkila, P J
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Coprinus cinereus
tryptophan synthase
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container_title Gene
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creator Skrzynia, C
Binninger, D M
Alspaugh, JA II
Pukkila, P J
description We utilized a cloned gene (TRP5 ) encoding tryptophan synthetase (TSase) from Saccharomyces cerevisiae to identify and clone the corresponding gene (TRP1 ) from the basidiomycete Coprinus cinereus . The primary nucleotide (nt) sequence of this gene was determined and compared to sequences from other filamentous fungi, as well as to other genes coding for TSase. A transformation assay was used to demonstrate that 321 nt, which do not include CAAT or TATAAA elements and precede the translation initiation codon, are sufficient for expression in a variety of chromosomal locations. The predicted translation product contains 702 amino acids (aa) and is very similar to other TSases, except in the region of aa 257-296 that connects the alpha and beta functional domains.
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subjects Coprinus cinereus
tryptophan synthase
title Molecular characterization of TRP1 , a gene coding for trytophan synthetase in the basidiomycete Coprinus cinereus
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