cDNA cloning of an mRNA encoding a sulfur-rich 10 kDa prolamin polypeptide in rice seeds
Using a rice maturing seed pUC9 expression library, we isolated a cDNA clone corresponding to 10 kDa sulfurrich prolamin by immunoscreening. A longer cDNA clone was obtained from a λgtll library by plaque hybridization using this (32)P-labeled cDNA as a probe. A polypeptide sequence composed of 134...
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| Veröffentlicht in: | Plant molecular biology 1989-02, Vol.12 (2), p.123-130 |
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| container_title | Plant molecular biology |
| container_volume | 12 |
| creator | MASUMURA, T SHIBATA, D HIBINO, T KATO, T KAWABE, K TAKETA, G TANAKA, K FUJII, S |
| description | Using a rice maturing seed pUC9 expression library, we isolated a cDNA clone corresponding to 10 kDa sulfurrich prolamin by immunoscreening. A longer cDNA clone was obtained from a λgtll library by plaque hybridization using this (32)P-labeled cDNA as a probe. A polypeptide sequence composed of 134 amino acids was deduced from the nucleotide sequence. A 24 amino acid signal peptide was assigned by computer calculation for the membrane spanning region and Edman sequencing of the purified mature polypeptide. Remarkably, 20% of methionine and 10% of cysteine were found in the mature polypeptide as well as high contents of glutamine, and hydrophobic amino acids. Part of the amino acid sequence was homologous with a conserved cysteine-rich region found in other plant prolamins. Two repeats of amino acid sequence were found in the polypeptide. |
| doi_str_mv | 10.1007/bf00020497 |
| format | Article |
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Dept. of Biochemistry</creatorcontrib><description>Using a rice maturing seed pUC9 expression library, we isolated a cDNA clone corresponding to 10 kDa sulfurrich prolamin by immunoscreening. A longer cDNA clone was obtained from a λgtll library by plaque hybridization using this (32)P-labeled cDNA as a probe. A polypeptide sequence composed of 134 amino acids was deduced from the nucleotide sequence. A 24 amino acid signal peptide was assigned by computer calculation for the membrane spanning region and Edman sequencing of the purified mature polypeptide. Remarkably, 20% of methionine and 10% of cysteine were found in the mature polypeptide as well as high contents of glutamine, and hydrophobic amino acids. Part of the amino acid sequence was homologous with a conserved cysteine-rich region found in other plant prolamins. 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Technologies ; Molecular cloning ; Oryza sativa ; peptide ; peptides ; peptidos ; prolaminas ; prolamine ; prolamines ; rna ; seeds ; semilla</subject><ispartof>Plant molecular biology, 1989-02, Vol.12 (2), p.123-130</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-9ac34a55cf373a6e6eb3fa757926c3bf46702ffb95d0410335cfb1b7889c3523</citedby><cites>FETCH-LOGICAL-c349t-9ac34a55cf373a6e6eb3fa757926c3bf46702ffb95d0410335cfb1b7889c3523</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27928,27929</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6743113$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24272791$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MASUMURA, T</creatorcontrib><creatorcontrib>SHIBATA, D</creatorcontrib><creatorcontrib>HIBINO, T</creatorcontrib><creatorcontrib>KATO, T</creatorcontrib><creatorcontrib>KAWABE, K</creatorcontrib><creatorcontrib>TAKETA, G</creatorcontrib><creatorcontrib>TANAKA, K</creatorcontrib><creatorcontrib>FUJII, S</creatorcontrib><creatorcontrib>Kyoto Prefectural Univ., Shimogamo, Kyoto (Japan). Dept. of Biochemistry</creatorcontrib><title>cDNA cloning of an mRNA encoding a sulfur-rich 10 kDa prolamin polypeptide in rice seeds</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>Using a rice maturing seed pUC9 expression library, we isolated a cDNA clone corresponding to 10 kDa sulfurrich prolamin by immunoscreening. A longer cDNA clone was obtained from a λgtll library by plaque hybridization using this (32)P-labeled cDNA as a probe. A polypeptide sequence composed of 134 amino acids was deduced from the nucleotide sequence. A 24 amino acid signal peptide was assigned by computer calculation for the membrane spanning region and Edman sequencing of the purified mature polypeptide. Remarkably, 20% of methionine and 10% of cysteine were found in the mature polypeptide as well as high contents of glutamine, and hydrophobic amino acids. Part of the amino acid sequence was homologous with a conserved cysteine-rich region found in other plant prolamins. Two repeats of amino acid sequence were found in the polypeptide.</description><subject>adn</subject><subject>arn</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>dna</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>graine</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular cloning</subject><subject>Oryza sativa</subject><subject>peptide</subject><subject>peptides</subject><subject>peptidos</subject><subject>prolaminas</subject><subject>prolamine</subject><subject>prolamines</subject><subject>rna</subject><subject>seeds</subject><subject>semilla</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><recordid>eNp90MtKAzEUBuAgiq3VjQ8gWYiIMHpymclkqdaqUCpIF-6GTCapo3Mz6Sz69qb04s7V4Rw-fpIfoXMCtwRA3OUWAChwKQ7QkMSCRTHQ9BANgSQi4pzQATrx_gsgcJYcowHlVFAhyRB96PHsHuuqbcpmgVuLVYPr93AyjW6L9U1h31e2d5Er9ScmgL_HCneurVRdNrhrq1VnumVZGBzWYAz2xhT-FB1ZVXlztp0jNJ88zR9founb8-vj_TTSjMtlJFWYKo61ZYKpxCQmZ1aJWEiaaJZbngig1uYyLoATYCzInOQiTaVmMWUjdLWJDS_66Y1fZnXptakq1Zi29xmJmeAgWIDX_0OekDQNJUKgNxuqXeu9MzbrXFkrt8oIZOvGs4fJrvGAL7a5fV6bYk93FQdwuQXKa1VZpxpd-r1LBGeEsL8cq9pMLVwgsymRqQRIRRp-_guJsY4l</recordid><startdate>198902</startdate><enddate>198902</enddate><creator>MASUMURA, T</creator><creator>SHIBATA, D</creator><creator>HIBINO, T</creator><creator>KATO, T</creator><creator>KAWABE, K</creator><creator>TAKETA, G</creator><creator>TANAKA, K</creator><creator>FUJII, S</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>198902</creationdate><title>cDNA cloning of an mRNA encoding a sulfur-rich 10 kDa prolamin polypeptide in rice seeds</title><author>MASUMURA, T ; SHIBATA, D ; HIBINO, T ; KATO, T ; KAWABE, K ; TAKETA, G ; TANAKA, K ; FUJII, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-9ac34a55cf373a6e6eb3fa757926c3bf46702ffb95d0410335cfb1b7889c3523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>adn</topic><topic>arn</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>dna</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>graine</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular cloning</topic><topic>Oryza sativa</topic><topic>peptide</topic><topic>peptides</topic><topic>peptidos</topic><topic>prolaminas</topic><topic>prolamine</topic><topic>prolamines</topic><topic>rna</topic><topic>seeds</topic><topic>semilla</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MASUMURA, T</creatorcontrib><creatorcontrib>SHIBATA, D</creatorcontrib><creatorcontrib>HIBINO, T</creatorcontrib><creatorcontrib>KATO, T</creatorcontrib><creatorcontrib>KAWABE, K</creatorcontrib><creatorcontrib>TAKETA, G</creatorcontrib><creatorcontrib>TANAKA, K</creatorcontrib><creatorcontrib>FUJII, S</creatorcontrib><creatorcontrib>Kyoto Prefectural Univ., Shimogamo, Kyoto (Japan). Dept. of Biochemistry</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MASUMURA, T</au><au>SHIBATA, D</au><au>HIBINO, T</au><au>KATO, T</au><au>KAWABE, K</au><au>TAKETA, G</au><au>TANAKA, K</au><au>FUJII, S</au><aucorp>Kyoto Prefectural Univ., Shimogamo, Kyoto (Japan). Dept. of Biochemistry</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>cDNA cloning of an mRNA encoding a sulfur-rich 10 kDa prolamin polypeptide in rice seeds</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1989-02</date><risdate>1989</risdate><volume>12</volume><issue>2</issue><spage>123</spage><epage>130</epage><pages>123-130</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><coden>PMBIDB</coden><abstract>Using a rice maturing seed pUC9 expression library, we isolated a cDNA clone corresponding to 10 kDa sulfurrich prolamin by immunoscreening. A longer cDNA clone was obtained from a λgtll library by plaque hybridization using this (32)P-labeled cDNA as a probe. A polypeptide sequence composed of 134 amino acids was deduced from the nucleotide sequence. A 24 amino acid signal peptide was assigned by computer calculation for the membrane spanning region and Edman sequencing of the purified mature polypeptide. Remarkably, 20% of methionine and 10% of cysteine were found in the mature polypeptide as well as high contents of glutamine, and hydrophobic amino acids. Part of the amino acid sequence was homologous with a conserved cysteine-rich region found in other plant prolamins. Two repeats of amino acid sequence were found in the polypeptide.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>24272791</pmid><doi>10.1007/bf00020497</doi><tpages>8</tpages></addata></record> |
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| ispartof | Plant molecular biology, 1989-02, Vol.12 (2), p.123-130 |
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| language | eng |
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| subjects | adn arn Biological and medical sciences Biotechnology dna Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics graine Methods. Procedures. Technologies Molecular cloning Oryza sativa peptide peptides peptidos prolaminas prolamine prolamines rna seeds semilla |
| title | cDNA cloning of an mRNA encoding a sulfur-rich 10 kDa prolamin polypeptide in rice seeds |
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