A Method for Preparing Permanent Mounts of Phytoplankton for Critical Microscopy and Cell Counting
A method is decribed for preparing permanent mounts of phytoplankton for critical microscopy and cell counting. Preserved phytoplankton are settled onto cover glasses (or slides), dehydrated by ethanol vapor substitution, and mounted in one of several ethanol-soluble resins. The resulting slides are...
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| Veröffentlicht in: | Limnol. Oceanogr.; (United States) 1981-01, Vol.26 (5), p.976-980 |
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| container_title | Limnol. Oceanogr.; (United States) |
| container_volume | 26 |
| creator | Crumpton, William G. Wetzel, Robert G. |
| description | A method is decribed for preparing permanent mounts of phytoplankton for critical microscopy and cell counting. Preserved phytoplankton are settled onto cover glasses (or slides), dehydrated by ethanol vapor substitution, and mounted in one of several ethanol-soluble resins. The resulting slides are permanent, quantitative, and of excellent optical quality. Resolution is very good and, with the appropriate resin, contrast is high enough to eliminate the need for phase shift or other contrast enhancement. |
| doi_str_mv | 10.4319/lo.1981.26.5.0976 |
| format | Article |
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Preserved phytoplankton are settled onto cover glasses (or slides), dehydrated by ethanol vapor substitution, and mounted in one of several ethanol-soluble resins. The resulting slides are permanent, quantitative, and of excellent optical quality. Resolution is very good and, with the appropriate resin, contrast is high enough to eliminate the need for phase shift or other contrast enhancement.</description><identifier>ISSN: 0024-3590</identifier><identifier>DOI: 10.4319/lo.1981.26.5.0976</identifier><language>eng</language><publisher>United States: American Society of Limnology and Oceanography</publisher><subject>520100 - Environment, Aquatic- Basic Studies- (-1989) ; ALCOHOLS ; AQUATIC ORGANISMS ; Cements ; Diatoms ; ENVIRONMENTAL SCIENCES ; ETHANOL ; Glass ; HYDROXY COMPOUNDS ; Light refraction ; Microscope slides ; MICROSCOPES ; MICROSCOPY ; OPTICAL MICROSCOPY ; ORGANIC COMPOUNDS ; PHYTOPLANKTON ; PLANKTON ; POPULATION DENSITY ; Resins ; RESOLUTION ; SENSITIVITY ; SPATIAL RESOLUTION ; Vapors</subject><ispartof>Limnol. 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Resolution is very good and, with the appropriate resin, contrast is high enough to eliminate the need for phase shift or other contrast enhancement.</description><subject>520100 - Environment, Aquatic- Basic Studies- (-1989)</subject><subject>ALCOHOLS</subject><subject>AQUATIC ORGANISMS</subject><subject>Cements</subject><subject>Diatoms</subject><subject>ENVIRONMENTAL SCIENCES</subject><subject>ETHANOL</subject><subject>Glass</subject><subject>HYDROXY COMPOUNDS</subject><subject>Light refraction</subject><subject>Microscope slides</subject><subject>MICROSCOPES</subject><subject>MICROSCOPY</subject><subject>OPTICAL MICROSCOPY</subject><subject>ORGANIC COMPOUNDS</subject><subject>PHYTOPLANKTON</subject><subject>PLANKTON</subject><subject>POPULATION DENSITY</subject><subject>Resins</subject><subject>RESOLUTION</subject><subject>SENSITIVITY</subject><subject>SPATIAL RESOLUTION</subject><subject>Vapors</subject><issn>0024-3590</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><recordid>eNotkD1PwzAYhD2ARCn8ACQGi4Gtwc4bO85YRXxJregAc-TYDnVJ7WC7Q_89hjLd8tzp7hC6oaSogDYPoy9oI2hR8oIVpKn5GZoRUlYLYA25QJcx7gghDWNshvolXpu09RoPPuBNMJMM1n3ijQl76YxLeO0PLkXsB7zZHpOfRum-knd_fBtsskqOeG1V8FH56Yil07g144jbX2POukLngxyjuf7XOfp4enxvXxart-fXdrla7Eoo06LROusAXHFhtIC-rwdJpWh4WVGTB3EONRDQA2hKRU-15n0FlQI59IYLmKO7U66PyXZR2WTUVnnnjEodEyVARTJ0f4Km4L8PJqZub6PKdfNYf4gdZVDTmlcZvD2Bu5h86KZg9zIcu1LkExnAD9j6bNo</recordid><startdate>19810101</startdate><enddate>19810101</enddate><creator>Crumpton, William G.</creator><creator>Wetzel, Robert G.</creator><general>American Society of Limnology and Oceanography</general><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>OTOTI</scope></search><sort><creationdate>19810101</creationdate><title>A Method for Preparing Permanent Mounts of Phytoplankton for Critical Microscopy and Cell Counting</title><author>Crumpton, William G. ; Wetzel, Robert G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j232t-9dd232f36c68ed83bb7fa1a896241e1986637303df3d118b1dd6b434c3afbe683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>520100 - Environment, Aquatic- Basic Studies- (-1989)</topic><topic>ALCOHOLS</topic><topic>AQUATIC ORGANISMS</topic><topic>Cements</topic><topic>Diatoms</topic><topic>ENVIRONMENTAL SCIENCES</topic><topic>ETHANOL</topic><topic>Glass</topic><topic>HYDROXY COMPOUNDS</topic><topic>Light refraction</topic><topic>Microscope slides</topic><topic>MICROSCOPES</topic><topic>MICROSCOPY</topic><topic>OPTICAL MICROSCOPY</topic><topic>ORGANIC COMPOUNDS</topic><topic>PHYTOPLANKTON</topic><topic>PLANKTON</topic><topic>POPULATION DENSITY</topic><topic>Resins</topic><topic>RESOLUTION</topic><topic>SENSITIVITY</topic><topic>SPATIAL RESOLUTION</topic><topic>Vapors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Crumpton, William G.</creatorcontrib><creatorcontrib>Wetzel, Robert G.</creatorcontrib><creatorcontrib>Michigan State Univ., East Lansing</creatorcontrib><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>OSTI.GOV</collection><jtitle>Limnol. Oceanogr.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Crumpton, William G.</au><au>Wetzel, Robert G.</au><aucorp>Michigan State Univ., East Lansing</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Method for Preparing Permanent Mounts of Phytoplankton for Critical Microscopy and Cell Counting</atitle><jtitle>Limnol. Oceanogr.; (United States)</jtitle><date>1981-01-01</date><risdate>1981</risdate><volume>26</volume><issue>5</issue><spage>976</spage><epage>980</epage><pages>976-980</pages><issn>0024-3590</issn><abstract>A method is decribed for preparing permanent mounts of phytoplankton for critical microscopy and cell counting. Preserved phytoplankton are settled onto cover glasses (or slides), dehydrated by ethanol vapor substitution, and mounted in one of several ethanol-soluble resins. The resulting slides are permanent, quantitative, and of excellent optical quality. Resolution is very good and, with the appropriate resin, contrast is high enough to eliminate the need for phase shift or other contrast enhancement.</abstract><cop>United States</cop><pub>American Society of Limnology and Oceanography</pub><doi>10.4319/lo.1981.26.5.0976</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0024-3590 |
| ispartof | Limnol. Oceanogr.; (United States), 1981-01, Vol.26 (5), p.976-980 |
| issn | 0024-3590 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15371764 |
| source | Jstor Complete Legacy; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | 520100 - Environment, Aquatic- Basic Studies- (-1989) ALCOHOLS AQUATIC ORGANISMS Cements Diatoms ENVIRONMENTAL SCIENCES ETHANOL Glass HYDROXY COMPOUNDS Light refraction Microscope slides MICROSCOPES MICROSCOPY OPTICAL MICROSCOPY ORGANIC COMPOUNDS PHYTOPLANKTON PLANKTON POPULATION DENSITY Resins RESOLUTION SENSITIVITY SPATIAL RESOLUTION Vapors |
| title | A Method for Preparing Permanent Mounts of Phytoplankton for Critical Microscopy and Cell Counting |
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