Purification of the 22 kDa protein substrate of botulinum ADP-ribosyltransferase C3 from porcine brain cytosol and its characterization as a GTP-binding protein highly homologous to the rho gene product
The 22 kDa protein substrate of botulinum ADP-ribosyltransferase C3 was purified from porcine brain cytosol by acetone precipitation, CM-Sephadex, octyl-Sepharose and TSK phenyl-5PW HPLC chromatography to apparent homogeneity. ADP-ribosylation of the protein was increased by guanine nucleotides (GTP...
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| Veröffentlicht in: | FEBS letters 1989-01, Vol.243 (1), p.70-76 |
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| creator | Braun, Ulrich Habermann, Barbara Just, Ingo Aktories, Klaus Vandekerckhove, Joel |
| description | The 22 kDa protein substrate of botulinum ADP-ribosyltransferase C3 was purified from porcine brain cytosol by acetone precipitation, CM-Sephadex, octyl-Sepharose and TSK phenyl-5PW HPLC chromatography to apparent homogeneity. ADP-ribosylation of the protein was increased by guanine nucleotides (GTP, GDP, GTPγS, each 100μM) but not by GMP, ATP or ATPγS. The purified 22 kDa protein bound maximally 0.9 mol [
35S]GTPγS and hydrolyzed GTP with the rate 0.007 mol per mol protein. Amino acid sequences were obtained from two tryptic peptides, selected from an in situ digestion of Immobilon electrotransferred, gel purified ADP-ribosylated substrate. The two sequences obtained, cover 23 residues from the corresponding sequences in human
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| doi_str_mv | 10.1016/0014-5793(89)81220-7 |
| format | Article |
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35S]GTPγS and hydrolyzed GTP with the rate 0.007 mol per mol protein. Amino acid sequences were obtained from two tryptic peptides, selected from an in situ digestion of Immobilon electrotransferred, gel purified ADP-ribosylated substrate. The two sequences obtained, cover 23 residues from the corresponding sequences in human
rho.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(89)81220-7</identifier><identifier>PMID: 2493391</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>ADP Ribose Transferases - metabolism ; ADP-ribosylation ; ADP-ribosyltransferase C3 ; Amino Acid Sequence ; Animals ; Botulinum Toxins - metabolism ; brain ; Brain - metabolism ; Cytosol - metabolism ; Gene product, rho ; GTP hydrolysis ; GTP Phosphohydrolases - metabolism ; GTP-binding protein ; GTP-Binding Proteins - genetics ; GTP-Binding Proteins - isolation & purification ; GTP-Binding Proteins - metabolism ; Guanosine 5'-O-(3-Thiotriphosphate) ; Guanosine Triphosphate - analogs & derivatives ; Guanosine Triphosphate - metabolism ; Humans ; Membrane Proteins ; Molecular Sequence Data ; rhoB GTP-Binding Protein ; Sequence Homology, Nucleic Acid ; Swine ; Thionucleotides - metabolism</subject><ispartof>FEBS letters, 1989-01, Vol.243 (1), p.70-76</ispartof><rights>1989</rights><rights>FEBS Letters 243 (1989) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4327-96790aee6c4166dcf2ffb5a8c2e2cb9542db761aa2c72a917f0dc3016c03c53</citedby><cites>FETCH-LOGICAL-c4327-96790aee6c4166dcf2ffb5a8c2e2cb9542db761aa2c72a917f0dc3016c03c53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014579389812207$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2493391$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Braun, Ulrich</creatorcontrib><creatorcontrib>Habermann, Barbara</creatorcontrib><creatorcontrib>Just, Ingo</creatorcontrib><creatorcontrib>Aktories, Klaus</creatorcontrib><creatorcontrib>Vandekerckhove, Joel</creatorcontrib><title>Purification of the 22 kDa protein substrate of botulinum ADP-ribosyltransferase C3 from porcine brain cytosol and its characterization as a GTP-binding protein highly homologous to the rho gene product</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The 22 kDa protein substrate of botulinum ADP-ribosyltransferase C3 was purified from porcine brain cytosol by acetone precipitation, CM-Sephadex, octyl-Sepharose and TSK phenyl-5PW HPLC chromatography to apparent homogeneity. ADP-ribosylation of the protein was increased by guanine nucleotides (GTP, GDP, GTPγS, each 100μM) but not by GMP, ATP or ATPγS. The purified 22 kDa protein bound maximally 0.9 mol [
35S]GTPγS and hydrolyzed GTP with the rate 0.007 mol per mol protein. Amino acid sequences were obtained from two tryptic peptides, selected from an in situ digestion of Immobilon electrotransferred, gel purified ADP-ribosylated substrate. The two sequences obtained, cover 23 residues from the corresponding sequences in human
rho.</description><subject>ADP Ribose Transferases - metabolism</subject><subject>ADP-ribosylation</subject><subject>ADP-ribosyltransferase C3</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Botulinum Toxins - metabolism</subject><subject>brain</subject><subject>Brain - metabolism</subject><subject>Cytosol - metabolism</subject><subject>Gene product, rho</subject><subject>GTP hydrolysis</subject><subject>GTP Phosphohydrolases - metabolism</subject><subject>GTP-binding protein</subject><subject>GTP-Binding Proteins - genetics</subject><subject>GTP-Binding Proteins - isolation & purification</subject><subject>GTP-Binding Proteins - metabolism</subject><subject>Guanosine 5'-O-(3-Thiotriphosphate)</subject><subject>Guanosine Triphosphate - analogs & derivatives</subject><subject>Guanosine Triphosphate - metabolism</subject><subject>Humans</subject><subject>Membrane Proteins</subject><subject>Molecular Sequence Data</subject><subject>rhoB GTP-Binding Protein</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Swine</subject><subject>Thionucleotides - metabolism</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUU2P0zAUtBBoKYV_AJJPCA4Bf-TLF6Slu12QVqISe7cc57kxJHGxHVD4ifwqnLbqEXGy_Oa9mdEMQi8peUcJLd8TQvOsqAR_U4u3NWWMZNUjtKJ1xTOel_VjtLqsPEXPQvhG0r-m4gpdsVxwLugK_dlN3hqrVbRuxM7g2AFmDH-_UfjgXQQ74jA1IXoVYcEbF6fejtOAr292mbeNC3Of0DEY8CoA3nBsvBvwwXltR8CNV4lDz9EF12M1ttjGgHWnvNIRvP19klYBK3z3sMsaO7Z23F_UO7vv-hl3bnC927sp4OiOLn3n8B6SQtpsJx2foydG9QFenN81-rq9fdh8yu6_3H3eXN9nOuesykRZCaIASp3Tsmy1YcY0hao1A6YbUeSsbaqSKsV0xZSglSGt5ilwTbgu-Bq9PrEm1R8ThCgHGzT0vRohmZO04EXNUrprlJ8WtXcheDDy4O2g_CwpkUuBcmlHLu3IWshjgbJKZ6_O_FMzQHs5OjeW8O0J_2V7mP-LU25vP7IFWOa1OE4XoQ8nIkhZ_bTgZdAWRg2t9aCjbJ39t9O_K_jCJQ</recordid><startdate>19890116</startdate><enddate>19890116</enddate><creator>Braun, Ulrich</creator><creator>Habermann, Barbara</creator><creator>Just, Ingo</creator><creator>Aktories, Klaus</creator><creator>Vandekerckhove, Joel</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope></search><sort><creationdate>19890116</creationdate><title>Purification of the 22 kDa protein substrate of botulinum ADP-ribosyltransferase C3 from porcine brain cytosol and its characterization as a GTP-binding protein highly homologous to the rho gene product</title><author>Braun, Ulrich ; Habermann, Barbara ; Just, Ingo ; Aktories, Klaus ; Vandekerckhove, Joel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4327-96790aee6c4166dcf2ffb5a8c2e2cb9542db761aa2c72a917f0dc3016c03c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>ADP Ribose Transferases - metabolism</topic><topic>ADP-ribosylation</topic><topic>ADP-ribosyltransferase C3</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Botulinum Toxins - metabolism</topic><topic>brain</topic><topic>Brain - metabolism</topic><topic>Cytosol - metabolism</topic><topic>Gene product, rho</topic><topic>GTP hydrolysis</topic><topic>GTP Phosphohydrolases - metabolism</topic><topic>GTP-binding protein</topic><topic>GTP-Binding Proteins - genetics</topic><topic>GTP-Binding Proteins - isolation & purification</topic><topic>GTP-Binding Proteins - metabolism</topic><topic>Guanosine 5'-O-(3-Thiotriphosphate)</topic><topic>Guanosine Triphosphate - analogs & derivatives</topic><topic>Guanosine Triphosphate - metabolism</topic><topic>Humans</topic><topic>Membrane Proteins</topic><topic>Molecular Sequence Data</topic><topic>rhoB GTP-Binding Protein</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Swine</topic><topic>Thionucleotides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Braun, Ulrich</creatorcontrib><creatorcontrib>Habermann, Barbara</creatorcontrib><creatorcontrib>Just, Ingo</creatorcontrib><creatorcontrib>Aktories, Klaus</creatorcontrib><creatorcontrib>Vandekerckhove, Joel</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Braun, Ulrich</au><au>Habermann, Barbara</au><au>Just, Ingo</au><au>Aktories, Klaus</au><au>Vandekerckhove, Joel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification of the 22 kDa protein substrate of botulinum ADP-ribosyltransferase C3 from porcine brain cytosol and its characterization as a GTP-binding protein highly homologous to the rho gene product</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1989-01-16</date><risdate>1989</risdate><volume>243</volume><issue>1</issue><spage>70</spage><epage>76</epage><pages>70-76</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>The 22 kDa protein substrate of botulinum ADP-ribosyltransferase C3 was purified from porcine brain cytosol by acetone precipitation, CM-Sephadex, octyl-Sepharose and TSK phenyl-5PW HPLC chromatography to apparent homogeneity. ADP-ribosylation of the protein was increased by guanine nucleotides (GTP, GDP, GTPγS, each 100μM) but not by GMP, ATP or ATPγS. The purified 22 kDa protein bound maximally 0.9 mol [
35S]GTPγS and hydrolyzed GTP with the rate 0.007 mol per mol protein. Amino acid sequences were obtained from two tryptic peptides, selected from an in situ digestion of Immobilon electrotransferred, gel purified ADP-ribosylated substrate. The two sequences obtained, cover 23 residues from the corresponding sequences in human
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| ispartof | FEBS letters, 1989-01, Vol.243 (1), p.70-76 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | ADP Ribose Transferases - metabolism ADP-ribosylation ADP-ribosyltransferase C3 Amino Acid Sequence Animals Botulinum Toxins - metabolism brain Brain - metabolism Cytosol - metabolism Gene product, rho GTP hydrolysis GTP Phosphohydrolases - metabolism GTP-binding protein GTP-Binding Proteins - genetics GTP-Binding Proteins - isolation & purification GTP-Binding Proteins - metabolism Guanosine 5'-O-(3-Thiotriphosphate) Guanosine Triphosphate - analogs & derivatives Guanosine Triphosphate - metabolism Humans Membrane Proteins Molecular Sequence Data rhoB GTP-Binding Protein Sequence Homology, Nucleic Acid Swine Thionucleotides - metabolism |
| title | Purification of the 22 kDa protein substrate of botulinum ADP-ribosyltransferase C3 from porcine brain cytosol and its characterization as a GTP-binding protein highly homologous to the rho gene product |
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