Retinal microglia are activated by systemic fungal infection
We determined whether systemic fungal infection could cause activation of retinal microglia and, therefore, could be potentially harmful for patients with retinal degenerative diseases. Activation of retinal microglia was measured in a model of sublethal invasive candidiasis in C57BL/6J mice by conf...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 2014-05, Vol.55 (6), p.3578-3585 |
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| container_title | Investigative ophthalmology & visual science |
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| creator | Maneu, Victoria Noailles, Agustina Megías, Javier Gómez-Vicente, Violeta Carpena, Núria Gil, M Luisa Gozalbo, Daniel Cuenca, Nicolás |
| description | We determined whether systemic fungal infection could cause activation of retinal microglia and, therefore, could be potentially harmful for patients with retinal degenerative diseases.
Activation of retinal microglia was measured in a model of sublethal invasive candidiasis in C57BL/6J mice by confocal immunofluorescence and flow cytometry analysis, using anti-CD11b, anti-Iba1, anti-MHCII, and anti-CD45 antibodies.
Systemic fungal infection causes activation of retinal microglia, with phenotypic changes in morphology, surface markers expression, and microglial relocation in retinal layers.
As an excessive or prolonged microglial activation may lead to chronic inflammation with severe pathological side effects, causing or worsening the course of retinal dystrophies, a systemic infection may represent a risk factor to be considered in patients with ocular neurodegenerative diseases, such as diabetic retinopathy, glaucoma, age-related macular degeneration, or retinitis pigmentosa. |
| doi_str_mv | 10.1167/iovs.14-14051 |
| format | Article |
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Activation of retinal microglia was measured in a model of sublethal invasive candidiasis in C57BL/6J mice by confocal immunofluorescence and flow cytometry analysis, using anti-CD11b, anti-Iba1, anti-MHCII, and anti-CD45 antibodies.
Systemic fungal infection causes activation of retinal microglia, with phenotypic changes in morphology, surface markers expression, and microglial relocation in retinal layers.
As an excessive or prolonged microglial activation may lead to chronic inflammation with severe pathological side effects, causing or worsening the course of retinal dystrophies, a systemic infection may represent a risk factor to be considered in patients with ocular neurodegenerative diseases, such as diabetic retinopathy, glaucoma, age-related macular degeneration, or retinitis pigmentosa.</description><identifier>ISSN: 1552-5783</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.14-14051</identifier><identifier>PMID: 24833742</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Axonal Transport ; Candidiasis - complications ; Candidiasis - metabolism ; Candidiasis - pathology ; Disease Models, Animal ; Female ; Flow Cytometry ; Immunohistochemistry ; Inflammation - metabolism ; Inflammation - pathology ; Mice ; Mice, Inbred C57BL ; Microglia - metabolism ; Microglia - pathology ; Microscopy, Confocal ; Retinal Degeneration - etiology ; Retinal Degeneration - metabolism ; Retinal Degeneration - pathology ; Retinal Ganglion Cells - metabolism ; Retinal Ganglion Cells - pathology</subject><ispartof>Investigative ophthalmology & visual science, 2014-05, Vol.55 (6), p.3578-3585</ispartof><rights>Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-e29886e664ce35b2fcd409f6efc263a3060d148fe3efd32ba974bd4a871f841b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24833742$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maneu, Victoria</creatorcontrib><creatorcontrib>Noailles, Agustina</creatorcontrib><creatorcontrib>Megías, Javier</creatorcontrib><creatorcontrib>Gómez-Vicente, Violeta</creatorcontrib><creatorcontrib>Carpena, Núria</creatorcontrib><creatorcontrib>Gil, M Luisa</creatorcontrib><creatorcontrib>Gozalbo, Daniel</creatorcontrib><creatorcontrib>Cuenca, Nicolás</creatorcontrib><title>Retinal microglia are activated by systemic fungal infection</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>We determined whether systemic fungal infection could cause activation of retinal microglia and, therefore, could be potentially harmful for patients with retinal degenerative diseases.
Activation of retinal microglia was measured in a model of sublethal invasive candidiasis in C57BL/6J mice by confocal immunofluorescence and flow cytometry analysis, using anti-CD11b, anti-Iba1, anti-MHCII, and anti-CD45 antibodies.
Systemic fungal infection causes activation of retinal microglia, with phenotypic changes in morphology, surface markers expression, and microglial relocation in retinal layers.
As an excessive or prolonged microglial activation may lead to chronic inflammation with severe pathological side effects, causing or worsening the course of retinal dystrophies, a systemic infection may represent a risk factor to be considered in patients with ocular neurodegenerative diseases, such as diabetic retinopathy, glaucoma, age-related macular degeneration, or retinitis pigmentosa.</description><subject>Animals</subject><subject>Axonal Transport</subject><subject>Candidiasis - complications</subject><subject>Candidiasis - metabolism</subject><subject>Candidiasis - pathology</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Immunohistochemistry</subject><subject>Inflammation - metabolism</subject><subject>Inflammation - pathology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microglia - metabolism</subject><subject>Microglia - pathology</subject><subject>Microscopy, Confocal</subject><subject>Retinal Degeneration - etiology</subject><subject>Retinal Degeneration - metabolism</subject><subject>Retinal Degeneration - pathology</subject><subject>Retinal Ganglion Cells - metabolism</subject><subject>Retinal Ganglion Cells - pathology</subject><issn>1552-5783</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkEtLw0AUhQdRbK0u3UqWblLnzitTcCOlPqAgiK6HyeROGcmjZpJC_r2preLqHjgfB-5HyDXQOYDK7kKzi3MQKQgq4YRMQUqWykzz0395Qi5i_KSUATB6TiZMaM4zwabk_g27UNsyqYJrm00ZbGJbTKzrws52WCT5kMQhdjj2ie_rzYiG2uPYN_UlOfO2jHh1vDPy8bh6Xz6n69enl-XDOnWcsy5FttBaoVLCIZc5864QdOEVescUt5wqWoDQHjn6grPcLjKRF8LqDLwWkPMZuT3sbtvmq8fYmSpEh2Vpa2z6aEByqVhGQY1oekDHb2Js0ZttGyrbDgao2Qsze2EGhPkRNvI3x-k-r7D4o38N8W8j4Gb7</recordid><startdate>20140515</startdate><enddate>20140515</enddate><creator>Maneu, Victoria</creator><creator>Noailles, Agustina</creator><creator>Megías, Javier</creator><creator>Gómez-Vicente, Violeta</creator><creator>Carpena, Núria</creator><creator>Gil, M Luisa</creator><creator>Gozalbo, Daniel</creator><creator>Cuenca, Nicolás</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140515</creationdate><title>Retinal microglia are activated by systemic fungal infection</title><author>Maneu, Victoria ; Noailles, Agustina ; Megías, Javier ; Gómez-Vicente, Violeta ; Carpena, Núria ; Gil, M Luisa ; Gozalbo, Daniel ; Cuenca, Nicolás</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-e29886e664ce35b2fcd409f6efc263a3060d148fe3efd32ba974bd4a871f841b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Axonal Transport</topic><topic>Candidiasis - complications</topic><topic>Candidiasis - metabolism</topic><topic>Candidiasis - pathology</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Immunohistochemistry</topic><topic>Inflammation - metabolism</topic><topic>Inflammation - pathology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microglia - metabolism</topic><topic>Microglia - pathology</topic><topic>Microscopy, Confocal</topic><topic>Retinal Degeneration - etiology</topic><topic>Retinal Degeneration - metabolism</topic><topic>Retinal Degeneration - pathology</topic><topic>Retinal Ganglion Cells - metabolism</topic><topic>Retinal Ganglion Cells - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maneu, Victoria</creatorcontrib><creatorcontrib>Noailles, Agustina</creatorcontrib><creatorcontrib>Megías, Javier</creatorcontrib><creatorcontrib>Gómez-Vicente, Violeta</creatorcontrib><creatorcontrib>Carpena, Núria</creatorcontrib><creatorcontrib>Gil, M Luisa</creatorcontrib><creatorcontrib>Gozalbo, Daniel</creatorcontrib><creatorcontrib>Cuenca, Nicolás</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maneu, Victoria</au><au>Noailles, Agustina</au><au>Megías, Javier</au><au>Gómez-Vicente, Violeta</au><au>Carpena, Núria</au><au>Gil, M Luisa</au><au>Gozalbo, Daniel</au><au>Cuenca, Nicolás</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Retinal microglia are activated by systemic fungal infection</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2014-05-15</date><risdate>2014</risdate><volume>55</volume><issue>6</issue><spage>3578</spage><epage>3585</epage><pages>3578-3585</pages><issn>1552-5783</issn><eissn>1552-5783</eissn><abstract>We determined whether systemic fungal infection could cause activation of retinal microglia and, therefore, could be potentially harmful for patients with retinal degenerative diseases.
Activation of retinal microglia was measured in a model of sublethal invasive candidiasis in C57BL/6J mice by confocal immunofluorescence and flow cytometry analysis, using anti-CD11b, anti-Iba1, anti-MHCII, and anti-CD45 antibodies.
Systemic fungal infection causes activation of retinal microglia, with phenotypic changes in morphology, surface markers expression, and microglial relocation in retinal layers.
As an excessive or prolonged microglial activation may lead to chronic inflammation with severe pathological side effects, causing or worsening the course of retinal dystrophies, a systemic infection may represent a risk factor to be considered in patients with ocular neurodegenerative diseases, such as diabetic retinopathy, glaucoma, age-related macular degeneration, or retinitis pigmentosa.</abstract><cop>United States</cop><pmid>24833742</pmid><doi>10.1167/iovs.14-14051</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Axonal Transport Candidiasis - complications Candidiasis - metabolism Candidiasis - pathology Disease Models, Animal Female Flow Cytometry Immunohistochemistry Inflammation - metabolism Inflammation - pathology Mice Mice, Inbred C57BL Microglia - metabolism Microglia - pathology Microscopy, Confocal Retinal Degeneration - etiology Retinal Degeneration - metabolism Retinal Degeneration - pathology Retinal Ganglion Cells - metabolism Retinal Ganglion Cells - pathology |
| title | Retinal microglia are activated by systemic fungal infection |
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