LIF/STAT3/SOCS3 Signaling Pathway in Murine Bone Marrow Stromal Cells Suppresses Osteoblast Differentiation
ABSTRACT Leukemia inhibitory factor (LIF) is a pleiotropic cytokine that belongs to the interleukin‐6 family and is expressed by multiple tissue types. This study analyzed the effect of LIF on osteoblast differentiation using primary murine bone marrow stromal cells (BMSCs). Colony‐forming unit‐oste...
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| Veröffentlicht in: | Journal of cellular biochemistry 2014-07, Vol.115 (7), p.1262-1268 |
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| container_title | Journal of cellular biochemistry |
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| creator | Matsushita, Kenta Itoh, Shousaku Ikeda, Shun Yamamoto, Yumiko Yamauchi, Yukako Hayashi, Mikako |
| description | ABSTRACT
Leukemia inhibitory factor (LIF) is a pleiotropic cytokine that belongs to the interleukin‐6 family and is expressed by multiple tissue types. This study analyzed the effect of LIF on osteoblast differentiation using primary murine bone marrow stromal cells (BMSCs). Colony‐forming unit‐osteoblast formation by BMSCs was significantly suppressed by LIF treatment. To clarify the mechanism underlying the LIF suppressive effect on osteoblast differentiation, we analyzed the downstream signaling pathway of LIF. LIF/signal transducer and activator of transcription 3 (STAT3) signaling induces the expression of suppressor of cytokine signaling 3 (SOCS3). SOCS3 knockdown experiments have previously demonstrated that short‐hairpin SOCS3‐BMSCs reversed the LIF suppressive effect. Our results demonstrated that LIF suppresses osteoblast differentiation through the LIF/STAT3/SOCS3 signaling pathway. J. Cell. Biochem. 115: 1262–1268, 2014. © 2014 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/jcb.24777 |
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Leukemia inhibitory factor (LIF) is a pleiotropic cytokine that belongs to the interleukin‐6 family and is expressed by multiple tissue types. This study analyzed the effect of LIF on osteoblast differentiation using primary murine bone marrow stromal cells (BMSCs). Colony‐forming unit‐osteoblast formation by BMSCs was significantly suppressed by LIF treatment. To clarify the mechanism underlying the LIF suppressive effect on osteoblast differentiation, we analyzed the downstream signaling pathway of LIF. LIF/signal transducer and activator of transcription 3 (STAT3) signaling induces the expression of suppressor of cytokine signaling 3 (SOCS3). SOCS3 knockdown experiments have previously demonstrated that short‐hairpin SOCS3‐BMSCs reversed the LIF suppressive effect. Our results demonstrated that LIF suppresses osteoblast differentiation through the LIF/STAT3/SOCS3 signaling pathway. J. Cell. Biochem. 115: 1262–1268, 2014. © 2014 Wiley Periodicals, Inc.</description><identifier>ISSN: 0730-2312</identifier><identifier>EISSN: 1097-4644</identifier><identifier>DOI: 10.1002/jcb.24777</identifier><identifier>PMID: 24464633</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>Animals ; beta Catenin - biosynthesis ; beta Catenin - metabolism ; Bone Marrow Cells ; BONE MARROW STROMAL CELLS ; CELL DIFFERENTIATION ; Cell Differentiation - drug effects ; Cells, Cultured ; CYTOKINE ; Leukemia Inhibitory Factor - pharmacology ; Mesenchymal Stromal Cells - metabolism ; Mice ; Mice, Inbred C57BL ; OSTEOBLASTS ; Osteoblasts - cytology ; RNA Interference ; RNA, Small Interfering ; SIGNAL TRANSDUCTION ; STAT3 Transcription Factor - biosynthesis ; STAT3 Transcription Factor - metabolism ; Suppressor of Cytokine Signaling 3 Protein ; Suppressor of Cytokine Signaling Proteins - biosynthesis ; Suppressor of Cytokine Signaling Proteins - genetics</subject><ispartof>Journal of cellular biochemistry, 2014-07, Vol.115 (7), p.1262-1268</ispartof><rights>2014 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4907-8e0d9b8b1a9af4759709ce43d8fffa3be48699f4b1ab4525702532c908221aff3</citedby><cites>FETCH-LOGICAL-c4907-8e0d9b8b1a9af4759709ce43d8fffa3be48699f4b1ab4525702532c908221aff3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcb.24777$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcb.24777$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24464633$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matsushita, Kenta</creatorcontrib><creatorcontrib>Itoh, Shousaku</creatorcontrib><creatorcontrib>Ikeda, Shun</creatorcontrib><creatorcontrib>Yamamoto, Yumiko</creatorcontrib><creatorcontrib>Yamauchi, Yukako</creatorcontrib><creatorcontrib>Hayashi, Mikako</creatorcontrib><title>LIF/STAT3/SOCS3 Signaling Pathway in Murine Bone Marrow Stromal Cells Suppresses Osteoblast Differentiation</title><title>Journal of cellular biochemistry</title><addtitle>J. Cell. Biochem</addtitle><description>ABSTRACT
Leukemia inhibitory factor (LIF) is a pleiotropic cytokine that belongs to the interleukin‐6 family and is expressed by multiple tissue types. This study analyzed the effect of LIF on osteoblast differentiation using primary murine bone marrow stromal cells (BMSCs). Colony‐forming unit‐osteoblast formation by BMSCs was significantly suppressed by LIF treatment. To clarify the mechanism underlying the LIF suppressive effect on osteoblast differentiation, we analyzed the downstream signaling pathway of LIF. LIF/signal transducer and activator of transcription 3 (STAT3) signaling induces the expression of suppressor of cytokine signaling 3 (SOCS3). SOCS3 knockdown experiments have previously demonstrated that short‐hairpin SOCS3‐BMSCs reversed the LIF suppressive effect. Our results demonstrated that LIF suppresses osteoblast differentiation through the LIF/STAT3/SOCS3 signaling pathway. J. Cell. Biochem. 115: 1262–1268, 2014. © 2014 Wiley Periodicals, Inc.</description><subject>Animals</subject><subject>beta Catenin - biosynthesis</subject><subject>beta Catenin - metabolism</subject><subject>Bone Marrow Cells</subject><subject>BONE MARROW STROMAL CELLS</subject><subject>CELL DIFFERENTIATION</subject><subject>Cell Differentiation - drug effects</subject><subject>Cells, Cultured</subject><subject>CYTOKINE</subject><subject>Leukemia Inhibitory Factor - pharmacology</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>OSTEOBLASTS</subject><subject>Osteoblasts - cytology</subject><subject>RNA Interference</subject><subject>RNA, Small Interfering</subject><subject>SIGNAL TRANSDUCTION</subject><subject>STAT3 Transcription Factor - biosynthesis</subject><subject>STAT3 Transcription Factor - metabolism</subject><subject>Suppressor of Cytokine Signaling 3 Protein</subject><subject>Suppressor of Cytokine Signaling Proteins - biosynthesis</subject><subject>Suppressor of Cytokine Signaling Proteins - genetics</subject><issn>0730-2312</issn><issn>1097-4644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0U9P2zAYBnALMUHHOPAFkCUu7BDqf4njIw2DMYUVlLIdLSe1mUsaFztR128_Q4HDpEm7-L383kev_ABwhNEZRoiMF019RhjnfAeMMBI8YRlju2CEOEUJoZjsg48hLBBCQlCyB_YJiyKjdAQey-vLcTU7n9FxNS0qCiv70KnWdg_wVvW_1moDbQdvBm87DScuPjfKe7eGVe_dUrWw0G0bYDWsVl6HoAOchl67ulWhhxfWGO1111vVW9d9Ah-MaoM-fJ0H4P7yy6z4mpTTq-vivEwaJhBPco3mos5rrIQyjKeCI9FoRue5MUbRWrM8E8KwCGqWkpQjklLSCJQTgpUx9ACcbnNX3j0NOvRyaUMT71SddkOQOKUsT1PM2X9QQrKcZIxEevIXXbjBx796UZjQnODnwM9b1XgXgtdGrrxdKr-RGMnnsmQsS76UFe3xa-JQL_X8Xb61E8F4C9a21Zt_J8lvxeQtMtlu2NjC7_cN5R9lxilP5c_vV7IsJuUd_sFkSf8Ae1Wq2Q</recordid><startdate>201407</startdate><enddate>201407</enddate><creator>Matsushita, Kenta</creator><creator>Itoh, Shousaku</creator><creator>Ikeda, Shun</creator><creator>Yamamoto, Yumiko</creator><creator>Yamauchi, Yukako</creator><creator>Hayashi, Mikako</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201407</creationdate><title>LIF/STAT3/SOCS3 Signaling Pathway in Murine Bone Marrow Stromal Cells Suppresses Osteoblast Differentiation</title><author>Matsushita, Kenta ; Itoh, Shousaku ; Ikeda, Shun ; Yamamoto, Yumiko ; Yamauchi, Yukako ; Hayashi, Mikako</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4907-8e0d9b8b1a9af4759709ce43d8fffa3be48699f4b1ab4525702532c908221aff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>beta Catenin - biosynthesis</topic><topic>beta Catenin - metabolism</topic><topic>Bone Marrow Cells</topic><topic>BONE MARROW STROMAL CELLS</topic><topic>CELL DIFFERENTIATION</topic><topic>Cell Differentiation - drug effects</topic><topic>Cells, Cultured</topic><topic>CYTOKINE</topic><topic>Leukemia Inhibitory Factor - pharmacology</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>OSTEOBLASTS</topic><topic>Osteoblasts - cytology</topic><topic>RNA Interference</topic><topic>RNA, Small Interfering</topic><topic>SIGNAL TRANSDUCTION</topic><topic>STAT3 Transcription Factor - biosynthesis</topic><topic>STAT3 Transcription Factor - metabolism</topic><topic>Suppressor of Cytokine Signaling 3 Protein</topic><topic>Suppressor of Cytokine Signaling Proteins - biosynthesis</topic><topic>Suppressor of Cytokine Signaling Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matsushita, Kenta</creatorcontrib><creatorcontrib>Itoh, Shousaku</creatorcontrib><creatorcontrib>Ikeda, Shun</creatorcontrib><creatorcontrib>Yamamoto, Yumiko</creatorcontrib><creatorcontrib>Yamauchi, Yukako</creatorcontrib><creatorcontrib>Hayashi, Mikako</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matsushita, Kenta</au><au>Itoh, Shousaku</au><au>Ikeda, Shun</au><au>Yamamoto, Yumiko</au><au>Yamauchi, Yukako</au><au>Hayashi, Mikako</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LIF/STAT3/SOCS3 Signaling Pathway in Murine Bone Marrow Stromal Cells Suppresses Osteoblast Differentiation</atitle><jtitle>Journal of cellular biochemistry</jtitle><addtitle>J. Cell. Biochem</addtitle><date>2014-07</date><risdate>2014</risdate><volume>115</volume><issue>7</issue><spage>1262</spage><epage>1268</epage><pages>1262-1268</pages><issn>0730-2312</issn><eissn>1097-4644</eissn><abstract>ABSTRACT
Leukemia inhibitory factor (LIF) is a pleiotropic cytokine that belongs to the interleukin‐6 family and is expressed by multiple tissue types. This study analyzed the effect of LIF on osteoblast differentiation using primary murine bone marrow stromal cells (BMSCs). Colony‐forming unit‐osteoblast formation by BMSCs was significantly suppressed by LIF treatment. To clarify the mechanism underlying the LIF suppressive effect on osteoblast differentiation, we analyzed the downstream signaling pathway of LIF. LIF/signal transducer and activator of transcription 3 (STAT3) signaling induces the expression of suppressor of cytokine signaling 3 (SOCS3). SOCS3 knockdown experiments have previously demonstrated that short‐hairpin SOCS3‐BMSCs reversed the LIF suppressive effect. Our results demonstrated that LIF suppresses osteoblast differentiation through the LIF/STAT3/SOCS3 signaling pathway. J. Cell. Biochem. 115: 1262–1268, 2014. © 2014 Wiley Periodicals, Inc.</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>24464633</pmid><doi>10.1002/jcb.24777</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals beta Catenin - biosynthesis beta Catenin - metabolism Bone Marrow Cells BONE MARROW STROMAL CELLS CELL DIFFERENTIATION Cell Differentiation - drug effects Cells, Cultured CYTOKINE Leukemia Inhibitory Factor - pharmacology Mesenchymal Stromal Cells - metabolism Mice Mice, Inbred C57BL OSTEOBLASTS Osteoblasts - cytology RNA Interference RNA, Small Interfering SIGNAL TRANSDUCTION STAT3 Transcription Factor - biosynthesis STAT3 Transcription Factor - metabolism Suppressor of Cytokine Signaling 3 Protein Suppressor of Cytokine Signaling Proteins - biosynthesis Suppressor of Cytokine Signaling Proteins - genetics |
| title | LIF/STAT3/SOCS3 Signaling Pathway in Murine Bone Marrow Stromal Cells Suppresses Osteoblast Differentiation |
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