Toward Fast Determination of Protein Stability Maps: Experimental and Theoretical Analysis of Mutants of a Nocardiopsis prasina Serine Protease

The stability of serine proteases is of major importance for their application in industrial processes. Here we study the determinants of the stability of a Nocardiopsis prasina serine protease using fast residual activity assays, a feature classification algorithm, and structure-based energy calcul...

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Veröffentlicht in:	Biochemistry (Easton) 2012-07, Vol.51 (26), p.5339-5347
Hauptverfasser:	Farrell, Damien, Webb, Helen, Johnston, Michael A, Poulsen, Thomas A, O’Meara, Fergal, Christensen, Lars L. H, Beier, Lars, Borchert, Torben V, Nielsen, Jens Erik
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinomycetales - enzymology Calorimetry, Differential Scanning Circular Dichroism Mutation Nocardiopsis Protein Stability Serine Proteases - chemistry Serine Proteases - genetics Serine Proteases - metabolism Structure-Activity Relationship
Online-Zugang:	Volltext
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