Temporal Variability of Genetic Patterns of Fecal Indicator Bacteria Collected from a Fixed Point in the River
By applying pulsed-field gel electrophoresis（PFGE）to the aquatic environment as a source tracking method of fecal pollution, the temporal variability of genetic patterns of Enterococcus faecium, as fecal indicator bacterium, was examined. Surface water samples were collected from a fixed point of th...
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| Veröffentlicht in: | Journal of Environmental Conservation Engineering 2013/10/20, Vol.42(10), pp.617-624 |
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| container_title | Journal of Environmental Conservation Engineering |
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| creator | FURUKAWA, Takashi SHIMAUCHI, Hidetaka SUZUKI, Yoshihiro |
| description | By applying pulsed-field gel electrophoresis（PFGE）to the aquatic environment as a source tracking method of fecal pollution, the temporal variability of genetic patterns of Enterococcus faecium, as fecal indicator bacterium, was examined. Surface water samples were collected from a fixed point of the Miyazaki municipal river during a two-month period. E. faecium strains were identified and collected from each water sample.Genotypes（PFGE types）of all E. faecium strains were analyzed by PFGE. Then, the similarities between the PFGE types obtained from the first day and those from the other days were compared using dendrogram analysis. The PFGE type of E. faecium was not significantly changed for the initial three days. After the seventh day, the dominant PFGE types were changed. The PFGE types of the 30th and 60th days were not very similar to those of the first day. It was indicated that genetic patterns of E. faecium might change to completely dissimilar patterns in a fixed point of the municipal river. Therefore, it is very important to consider the temporal variability of genetic pattern of fecal indicator bacteria for source tracking of fecal pollution. |
| doi_str_mv | 10.5956/jriet.42.617 |
| format | Article |
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Surface water samples were collected from a fixed point of the Miyazaki municipal river during a two-month period. E. faecium strains were identified and collected from each water sample.Genotypes（PFGE types）of all E. faecium strains were analyzed by PFGE. Then, the similarities between the PFGE types obtained from the first day and those from the other days were compared using dendrogram analysis. The PFGE type of E. faecium was not significantly changed for the initial three days. After the seventh day, the dominant PFGE types were changed. The PFGE types of the 30th and 60th days were not very similar to those of the first day. It was indicated that genetic patterns of E. faecium might change to completely dissimilar patterns in a fixed point of the municipal river. Therefore, it is very important to consider the temporal variability of genetic pattern of fecal indicator bacteria for source tracking of fecal pollution.</description><identifier>ISSN: 0388-9459</identifier><identifier>EISSN: 1882-8590</identifier><identifier>DOI: 10.5956/jriet.42.617</identifier><language>eng</language><publisher>Society of Environmental Conservation Engineering</publisher><subject>dendrogram analysis ; Enterococcus faecium ; Freshwater ; genetic variability ; Microbial source tracking ; PFGE</subject><ispartof>Journal of Environmental Conservation Engineering, 2013/10/20, Vol.42(10), pp.617-624</ispartof><rights>2013 Society of Environmental Conservation Engineering</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1657-68d987e9c63d92e38d1adfe70ddd44b71c613db928741ad3553157f8001bda303</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids></links><search><creatorcontrib>FURUKAWA, Takashi</creatorcontrib><creatorcontrib>SHIMAUCHI, Hidetaka</creatorcontrib><creatorcontrib>SUZUKI, Yoshihiro</creatorcontrib><title>Temporal Variability of Genetic Patterns of Fecal Indicator Bacteria Collected from a Fixed Point in the River</title><title>Journal of Environmental Conservation Engineering</title><addtitle>J. Env. cons. eng</addtitle><description>By applying pulsed-field gel electrophoresis（PFGE）to the aquatic environment as a source tracking method of fecal pollution, the temporal variability of genetic patterns of Enterococcus faecium, as fecal indicator bacterium, was examined. Surface water samples were collected from a fixed point of the Miyazaki municipal river during a two-month period. E. faecium strains were identified and collected from each water sample.Genotypes（PFGE types）of all E. faecium strains were analyzed by PFGE. Then, the similarities between the PFGE types obtained from the first day and those from the other days were compared using dendrogram analysis. The PFGE type of E. faecium was not significantly changed for the initial three days. After the seventh day, the dominant PFGE types were changed. The PFGE types of the 30th and 60th days were not very similar to those of the first day. It was indicated that genetic patterns of E. faecium might change to completely dissimilar patterns in a fixed point of the municipal river. Therefore, it is very important to consider the temporal variability of genetic pattern of fecal indicator bacteria for source tracking of fecal pollution.</description><subject>dendrogram analysis</subject><subject>Enterococcus faecium</subject><subject>Freshwater</subject><subject>genetic variability</subject><subject>Microbial source tracking</subject><subject>PFGE</subject><issn>0388-9459</issn><issn>1882-8590</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNpFkM1OJCEURolxEjvaOx-ApQurBwooYKd2bDUxmc7EmS2h4JZiqosW0NhvP2gbZ3V_vnPv4iB0SslCaNH9fE4ByoK3i47KAzSjSrWNEpocohlhSjWaC32E5jmHnpCWt4xoOkPTA2y2MdkR_7Up2D6MoexwHPANTFCCw2tbCqQpf-xW4Cp4N_ngbIkJX1lXs2DxMo4j1N7jIcUNtngV3uuwjmEqOEy4PAH-Hd4gnaAfgx0zzL_qMfqzun5Y3jb3v27ulpf3jaOdkE2nvFYStOuY1y0w5an1A0jivee8l9R1lPlet0rymjAhGBVyUITQ3ltG2DE62__dpvjyCrmYTcgOxtFOEF-zoYJxxaXkqqLne9SlmHOCwWxT2Ni0M5SYD7Pm06zhralmK36xx59zsY_wDdtUbY3wH67X-5PvyD3ZZGBi_wDDQYRC</recordid><startdate>20130101</startdate><enddate>20130101</enddate><creator>FURUKAWA, Takashi</creator><creator>SHIMAUCHI, Hidetaka</creator><creator>SUZUKI, Yoshihiro</creator><general>Society of Environmental Conservation Engineering</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7QL</scope><scope>7ST</scope><scope>7TV</scope><scope>7U6</scope><scope>7UA</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H96</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20130101</creationdate><title>Temporal Variability of Genetic Patterns of Fecal Indicator Bacteria Collected from a Fixed Point in the River</title><author>FURUKAWA, Takashi ; SHIMAUCHI, Hidetaka ; SUZUKI, Yoshihiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1657-68d987e9c63d92e38d1adfe70ddd44b71c613db928741ad3553157f8001bda303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>dendrogram analysis</topic><topic>Enterococcus faecium</topic><topic>Freshwater</topic><topic>genetic variability</topic><topic>Microbial source tracking</topic><topic>PFGE</topic><toplevel>online_resources</toplevel><creatorcontrib>FURUKAWA, Takashi</creatorcontrib><creatorcontrib>SHIMAUCHI, Hidetaka</creatorcontrib><creatorcontrib>SUZUKI, Yoshihiro</creatorcontrib><collection>CrossRef</collection><collection>Aqualine</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environment Abstracts</collection><collection>Pollution Abstracts</collection><collection>Sustainability Science Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 2: Ocean Technology, Policy & Non-Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of Environmental Conservation Engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>FURUKAWA, Takashi</au><au>SHIMAUCHI, Hidetaka</au><au>SUZUKI, Yoshihiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Temporal Variability of Genetic Patterns of Fecal Indicator Bacteria Collected from a Fixed Point in the River</atitle><jtitle>Journal of Environmental Conservation Engineering</jtitle><addtitle>J. Env. cons. eng</addtitle><date>2013-01-01</date><risdate>2013</risdate><volume>42</volume><issue>10</issue><spage>617</spage><epage>624</epage><pages>617-624</pages><issn>0388-9459</issn><eissn>1882-8590</eissn><abstract>By applying pulsed-field gel electrophoresis（PFGE）to the aquatic environment as a source tracking method of fecal pollution, the temporal variability of genetic patterns of Enterococcus faecium, as fecal indicator bacterium, was examined. Surface water samples were collected from a fixed point of the Miyazaki municipal river during a two-month period. E. faecium strains were identified and collected from each water sample.Genotypes（PFGE types）of all E. faecium strains were analyzed by PFGE. Then, the similarities between the PFGE types obtained from the first day and those from the other days were compared using dendrogram analysis. The PFGE type of E. faecium was not significantly changed for the initial three days. After the seventh day, the dominant PFGE types were changed. The PFGE types of the 30th and 60th days were not very similar to those of the first day. It was indicated that genetic patterns of E. faecium might change to completely dissimilar patterns in a fixed point of the municipal river. Therefore, it is very important to consider the temporal variability of genetic pattern of fecal indicator bacteria for source tracking of fecal pollution.</abstract><pub>Society of Environmental Conservation Engineering</pub><doi>10.5956/jriet.42.617</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | dendrogram analysis Enterococcus faecium Freshwater genetic variability Microbial source tracking PFGE |
| title | Temporal Variability of Genetic Patterns of Fecal Indicator Bacteria Collected from a Fixed Point in the River |
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