Production of 1,3-propanediol from glycerol using the newly isolated Klebsiella pneumoniae J2B

•The new isolate could efficiently produce PDO without forming pathogenic LPS.•Deletion of ldhA abolished lactate formation but with enhanced production of BDO.•Maximum PDO production was 58.0g/L with the productivity of 1.3g/L/h.•Product inhibition was considered major bottleneck for improving PDO...

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