Biosynthesis of Glucosyl Glycerol, a Compatible Solute, Using Intermolecular Transglycosylation Activity of Amylosucrase from Methylobacillus flagellatus KT

A putative α-amylase gene (accession number, CP000284) of Methylobacillus flagellatus KT ATCC51484 was cloned in Escherichia coli , and its gene product was expressed and characterized. The purified recombinant enzyme (MFAS) displayed a typical amylosucrase (ASase) activity by the demonstration of m...

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Veröffentlicht in:	Applied biochemistry and biotechnology 2014-06, Vol.173 (4), p.904-917
Hauptverfasser:	Jeong, Jin-Woo, Seo, Dong-Ho, Jung, Jong-Hyun, Park, Ji-Hae, Baek, Nam-In, Kim, Myo-Jeong, Park, Cheon-Seok
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - chemistry Biochemistry Biological and medical sciences Biosynthesis Biotechnology Chemistry Chemistry and Materials Science Cloning E coli Enzymes Escherichia coli Fundamental and applied biological sciences. Psychology Glucosides - biosynthesis Glucosyltransferases - biosynthesis Glucosyltransferases - chemistry Glucosyltransferases - isolation & purification Glycerol Hydrolysis Liquid chromatography Methylobacillus - enzymology Methylobacillus flagellatus Thin layer chromatography
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