Bacterial and Viral Sialidases: Contribution of the Conserved Active Site Glutamate to Catalysis

Mutagenesis of the conserved glutamic acid of influenza type A (E277) and Micromonospora viridifaciens (E260) sialidases was performed to probe the contribution of this strictly conserved residue to catalysis. Kinetic studies of the E260D and E260C M. viridifaciens mutant enzymes reveal that the ove...

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Veröffentlicht in:	Biochemistry (Easton) 2012-01, Vol.51 (1), p.433-441
Hauptverfasser:	Chan, Jefferson, Watson, Jacqueline N, Lu, April, Cerda, Viviana C, Borgford, Thor J, Bennet, Andrew J
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteria Baculoviridae - enzymology Baculoviridae - genetics Catalysis Catalytic Domain - genetics Clostridium perfringens - enzymology Clostridium perfringens - genetics Conserved Sequence Crystallography, X-Ray Deuterium Exchange Measurement Glutamic Acid - chemistry Humans Influenza A virus - enzymology Influenza A virus - genetics Micromonospora Micromonospora - enzymology Micromonospora - genetics Mutagenesis, Site-Directed Neuraminidase - chemistry Neuraminidase - metabolism Substrate Specificity - genetics
Online-Zugang:	Volltext
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