Anoctamin 1 Positive Esophageal Interstitial Cajal Cells in Late Stage Human Embryos
ABSTRACT Interstitial cells of Cajal (ICCs) are located in various smooth muscle organs and act as pacemaker cells, or ensure neuromodulation or mechanosensory roles. The study aims to investigate functional states of human ICCs in morphogenesis, focusing on the anoctamin 1 phenotype. The investigat...
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| Veröffentlicht in: | Anatomical record (Hoboken, N.J. : 2007) N.J. : 2007), 2014-02, Vol.297 (2), p.301-307 |
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| creator | Rusu, Mugurel Constantin Poalelungi, Cristian Viorel Vrapciu, Alexandra Diana Păduraru, Luminiţa Didilescu, Andreea Cristiana Stan, Cristinel Ionel |
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Interstitial cells of Cajal (ICCs) are located in various smooth muscle organs and act as pacemaker cells, or ensure neuromodulation or mechanosensory roles. The study aims to investigate functional states of human ICCs in morphogenesis, focusing on the anoctamin 1 phenotype. The investigation was performed in five late stage human embryos with lengths varying between 23 and 29 mm. Immunohistochemistry on paraffin embedded specimens was performed for a series of antibodies: α‐smooth muscle actin (α‐SMA), desmin, CD31, CD34, CD117/c‐kit, DOG1, and nestin. Longitudinal and circular muscle layers were α‐SMA+/desmin+/nestin+. An immature microvascular layer located in the inner submucosa was CD34+/CD31+/α‐SMA+/nestin+; endothelial tip cells were supporting active processes of sprouting angiogenesis. A CD34+/CD31‐ mesenchymal network was found in the circular muscle layer. CD117/c‐kit+ multipolar ICCs with dichotomizing processes were found mostly in the myenteric plexus layer; processes were configuring a network within the circular muscle layer where intramuscular ICCs were scarcely found. A strong DOG1+ reaction was found for the ICCs of the myenteric plexus layer apposed on the outer surface of the circular muscle layer, and for the intramuscular ICCs. The evidence of a sublayer of DOG1+ myenteric ICCs is suggestive for a subpopulation of ICCs being qualified for pacemaking at this early developmental stage. Anat Rec, 297:301–307, 2014. © 2013 Wiley Periodicals, Inc. |
| doi_str_mv | 10.1002/ar.22837 |
| format | Article |
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Interstitial cells of Cajal (ICCs) are located in various smooth muscle organs and act as pacemaker cells, or ensure neuromodulation or mechanosensory roles. The study aims to investigate functional states of human ICCs in morphogenesis, focusing on the anoctamin 1 phenotype. The investigation was performed in five late stage human embryos with lengths varying between 23 and 29 mm. Immunohistochemistry on paraffin embedded specimens was performed for a series of antibodies: α‐smooth muscle actin (α‐SMA), desmin, CD31, CD34, CD117/c‐kit, DOG1, and nestin. Longitudinal and circular muscle layers were α‐SMA+/desmin+/nestin+. An immature microvascular layer located in the inner submucosa was CD34+/CD31+/α‐SMA+/nestin+; endothelial tip cells were supporting active processes of sprouting angiogenesis. A CD34+/CD31‐ mesenchymal network was found in the circular muscle layer. CD117/c‐kit+ multipolar ICCs with dichotomizing processes were found mostly in the myenteric plexus layer; processes were configuring a network within the circular muscle layer where intramuscular ICCs were scarcely found. A strong DOG1+ reaction was found for the ICCs of the myenteric plexus layer apposed on the outer surface of the circular muscle layer, and for the intramuscular ICCs. The evidence of a sublayer of DOG1+ myenteric ICCs is suggestive for a subpopulation of ICCs being qualified for pacemaking at this early developmental stage. Anat Rec, 297:301–307, 2014. © 2013 Wiley Periodicals, Inc.</description><identifier>ISSN: 1932-8486</identifier><identifier>EISSN: 1932-8494</identifier><identifier>DOI: 10.1002/ar.22837</identifier><identifier>PMID: 24243583</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Actins - metabolism ; Anoctamin-1 ; Antigens, CD34 - metabolism ; CD31 ; CD34 ; Chloride Channels - metabolism ; Desmin - metabolism ; DOG1 ; embryology ; endothelial tip cells ; Esophagus - cytology ; Esophagus - embryology ; Esophagus - metabolism ; Humans ; Interstitial Cells of Cajal - cytology ; Interstitial Cells of Cajal - metabolism ; Morphogenesis - physiology ; Muscle, Smooth - cytology ; Muscle, Smooth - embryology ; Muscle, Smooth - metabolism ; Neoplasm Proteins - metabolism ; nestin ; Nestin - metabolism ; Platelet Endothelial Cell Adhesion Molecule-1 - metabolism ; smooth muscle actin</subject><ispartof>Anatomical record (Hoboken, N.J. : 2007), 2014-02, Vol.297 (2), p.301-307</ispartof><rights>Copyright © 2013 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4167-45119dda07760570f7ce304ac8a4150f8f390a769ed69f01863754c7808f3dd83</citedby><cites>FETCH-LOGICAL-c4167-45119dda07760570f7ce304ac8a4150f8f390a769ed69f01863754c7808f3dd83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Far.22837$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Far.22837$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24243583$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rusu, Mugurel Constantin</creatorcontrib><creatorcontrib>Poalelungi, Cristian Viorel</creatorcontrib><creatorcontrib>Vrapciu, Alexandra Diana</creatorcontrib><creatorcontrib>Păduraru, Luminiţa</creatorcontrib><creatorcontrib>Didilescu, Andreea Cristiana</creatorcontrib><creatorcontrib>Stan, Cristinel Ionel</creatorcontrib><title>Anoctamin 1 Positive Esophageal Interstitial Cajal Cells in Late Stage Human Embryos</title><title>Anatomical record (Hoboken, N.J. : 2007)</title><addtitle>Anat Rec (Hoboken)</addtitle><description>ABSTRACT
Interstitial cells of Cajal (ICCs) are located in various smooth muscle organs and act as pacemaker cells, or ensure neuromodulation or mechanosensory roles. The study aims to investigate functional states of human ICCs in morphogenesis, focusing on the anoctamin 1 phenotype. The investigation was performed in five late stage human embryos with lengths varying between 23 and 29 mm. Immunohistochemistry on paraffin embedded specimens was performed for a series of antibodies: α‐smooth muscle actin (α‐SMA), desmin, CD31, CD34, CD117/c‐kit, DOG1, and nestin. Longitudinal and circular muscle layers were α‐SMA+/desmin+/nestin+. An immature microvascular layer located in the inner submucosa was CD34+/CD31+/α‐SMA+/nestin+; endothelial tip cells were supporting active processes of sprouting angiogenesis. A CD34+/CD31‐ mesenchymal network was found in the circular muscle layer. CD117/c‐kit+ multipolar ICCs with dichotomizing processes were found mostly in the myenteric plexus layer; processes were configuring a network within the circular muscle layer where intramuscular ICCs were scarcely found. A strong DOG1+ reaction was found for the ICCs of the myenteric plexus layer apposed on the outer surface of the circular muscle layer, and for the intramuscular ICCs. The evidence of a sublayer of DOG1+ myenteric ICCs is suggestive for a subpopulation of ICCs being qualified for pacemaking at this early developmental stage. Anat Rec, 297:301–307, 2014. © 2013 Wiley Periodicals, Inc.</description><subject>Actins - metabolism</subject><subject>Anoctamin-1</subject><subject>Antigens, CD34 - metabolism</subject><subject>CD31</subject><subject>CD34</subject><subject>Chloride Channels - metabolism</subject><subject>Desmin - metabolism</subject><subject>DOG1</subject><subject>embryology</subject><subject>endothelial tip cells</subject><subject>Esophagus - cytology</subject><subject>Esophagus - embryology</subject><subject>Esophagus - metabolism</subject><subject>Humans</subject><subject>Interstitial Cells of Cajal - cytology</subject><subject>Interstitial Cells of Cajal - metabolism</subject><subject>Morphogenesis - physiology</subject><subject>Muscle, Smooth - cytology</subject><subject>Muscle, Smooth - embryology</subject><subject>Muscle, Smooth - metabolism</subject><subject>Neoplasm Proteins - metabolism</subject><subject>nestin</subject><subject>Nestin - metabolism</subject><subject>Platelet Endothelial Cell Adhesion Molecule-1 - metabolism</subject><subject>smooth muscle actin</subject><issn>1932-8486</issn><issn>1932-8494</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkdFKwzAUhoMobk7BJ5CCN950njRJk1yOMd1goOi8DlmbakfbzKRV9vZmbioIgjc5ycl3PhJ-hM4xDDFAcq3dMEkE4QeojyVJYkElPfzei7SHTrxfATAKkhyjXkITSpggfbQYNTZrdV02EY7urS_b8s1EE2_XL_rZ6CqaNa1xvg39cBjr1XY1VeWjMDHXrYke2wBG067WTTSpl25j_Sk6KnTlzdm-DtDTzWQxnsbzu9vZeDSPM4pTHlOGscxzDZynwDgUPDMEqM6EpphBIQoiQfNUmjyVBWCREs5oxgWEmzwXZICudt61s6-d8a2qS5-F1-nG2M4rzAgViZSc_gMFiqmQggT08he6sp1rwkcUppxLxiVlP8LMWe-dKdTalbV2G4VBbUNR2qnPUAJ6sRd2y9rk3-BXCgGId8B7WZnNnyI1etgJPwBkvpGG</recordid><startdate>201402</startdate><enddate>201402</enddate><creator>Rusu, Mugurel Constantin</creator><creator>Poalelungi, Cristian Viorel</creator><creator>Vrapciu, Alexandra Diana</creator><creator>Păduraru, Luminiţa</creator><creator>Didilescu, Andreea Cristiana</creator><creator>Stan, Cristinel Ionel</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TS</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201402</creationdate><title>Anoctamin 1 Positive Esophageal Interstitial Cajal Cells in Late Stage Human Embryos</title><author>Rusu, Mugurel Constantin ; Poalelungi, Cristian Viorel ; Vrapciu, Alexandra Diana ; Păduraru, Luminiţa ; Didilescu, Andreea Cristiana ; Stan, Cristinel Ionel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4167-45119dda07760570f7ce304ac8a4150f8f390a769ed69f01863754c7808f3dd83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Actins - metabolism</topic><topic>Anoctamin-1</topic><topic>Antigens, CD34 - metabolism</topic><topic>CD31</topic><topic>CD34</topic><topic>Chloride Channels - metabolism</topic><topic>Desmin - metabolism</topic><topic>DOG1</topic><topic>embryology</topic><topic>endothelial tip cells</topic><topic>Esophagus - cytology</topic><topic>Esophagus - embryology</topic><topic>Esophagus - metabolism</topic><topic>Humans</topic><topic>Interstitial Cells of Cajal - cytology</topic><topic>Interstitial Cells of Cajal - metabolism</topic><topic>Morphogenesis - physiology</topic><topic>Muscle, Smooth - cytology</topic><topic>Muscle, Smooth - embryology</topic><topic>Muscle, Smooth - metabolism</topic><topic>Neoplasm Proteins - metabolism</topic><topic>nestin</topic><topic>Nestin - metabolism</topic><topic>Platelet Endothelial Cell Adhesion Molecule-1 - metabolism</topic><topic>smooth muscle actin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rusu, Mugurel Constantin</creatorcontrib><creatorcontrib>Poalelungi, Cristian Viorel</creatorcontrib><creatorcontrib>Vrapciu, Alexandra Diana</creatorcontrib><creatorcontrib>Păduraru, Luminiţa</creatorcontrib><creatorcontrib>Didilescu, Andreea Cristiana</creatorcontrib><creatorcontrib>Stan, Cristinel Ionel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Anatomical record (Hoboken, N.J. : 2007)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rusu, Mugurel Constantin</au><au>Poalelungi, Cristian Viorel</au><au>Vrapciu, Alexandra Diana</au><au>Păduraru, Luminiţa</au><au>Didilescu, Andreea Cristiana</au><au>Stan, Cristinel Ionel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anoctamin 1 Positive Esophageal Interstitial Cajal Cells in Late Stage Human Embryos</atitle><jtitle>Anatomical record (Hoboken, N.J. : 2007)</jtitle><addtitle>Anat Rec (Hoboken)</addtitle><date>2014-02</date><risdate>2014</risdate><volume>297</volume><issue>2</issue><spage>301</spage><epage>307</epage><pages>301-307</pages><issn>1932-8486</issn><eissn>1932-8494</eissn><abstract>ABSTRACT
Interstitial cells of Cajal (ICCs) are located in various smooth muscle organs and act as pacemaker cells, or ensure neuromodulation or mechanosensory roles. The study aims to investigate functional states of human ICCs in morphogenesis, focusing on the anoctamin 1 phenotype. The investigation was performed in five late stage human embryos with lengths varying between 23 and 29 mm. Immunohistochemistry on paraffin embedded specimens was performed for a series of antibodies: α‐smooth muscle actin (α‐SMA), desmin, CD31, CD34, CD117/c‐kit, DOG1, and nestin. Longitudinal and circular muscle layers were α‐SMA+/desmin+/nestin+. An immature microvascular layer located in the inner submucosa was CD34+/CD31+/α‐SMA+/nestin+; endothelial tip cells were supporting active processes of sprouting angiogenesis. A CD34+/CD31‐ mesenchymal network was found in the circular muscle layer. CD117/c‐kit+ multipolar ICCs with dichotomizing processes were found mostly in the myenteric plexus layer; processes were configuring a network within the circular muscle layer where intramuscular ICCs were scarcely found. A strong DOG1+ reaction was found for the ICCs of the myenteric plexus layer apposed on the outer surface of the circular muscle layer, and for the intramuscular ICCs. The evidence of a sublayer of DOG1+ myenteric ICCs is suggestive for a subpopulation of ICCs being qualified for pacemaking at this early developmental stage. Anat Rec, 297:301–307, 2014. © 2013 Wiley Periodicals, Inc.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>24243583</pmid><doi>10.1002/ar.22837</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Anatomical record (Hoboken, N.J. : 2007), 2014-02, Vol.297 (2), p.301-307 |
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| subjects | Actins - metabolism Anoctamin-1 Antigens, CD34 - metabolism CD31 CD34 Chloride Channels - metabolism Desmin - metabolism DOG1 embryology endothelial tip cells Esophagus - cytology Esophagus - embryology Esophagus - metabolism Humans Interstitial Cells of Cajal - cytology Interstitial Cells of Cajal - metabolism Morphogenesis - physiology Muscle, Smooth - cytology Muscle, Smooth - embryology Muscle, Smooth - metabolism Neoplasm Proteins - metabolism nestin Nestin - metabolism Platelet Endothelial Cell Adhesion Molecule-1 - metabolism smooth muscle actin |
| title | Anoctamin 1 Positive Esophageal Interstitial Cajal Cells in Late Stage Human Embryos |
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