Cytisus villosus from Northeastern Algeria is nodulated by genetically diverse Bradyrhizobium strains
Fifty-one rhizobial strains isolated from root nodules of Cytisus villosus growing in Northeastern Algeria were characterized by genomic and phenotypic analyses. Isolates were grouped into sixteen different patterns by PCR-RAPD. The phylogenetic status of one representative isolate from each pattern...
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| Veröffentlicht in: | Antonie van Leeuwenhoek 2014-06, Vol.105 (6), p.1121-1129 |
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| creator | Ahnia, Hadjira Boulila, Farida Boulila, Abdelghani Boucheffa, Karima Durán, David Bourebaba, Yasmina Salmi, Adouda Imperial, Juan Ruiz-Argüeso, Tomás Rey, Luis |
| description | Fifty-one rhizobial strains isolated from root nodules of Cytisus villosus growing in Northeastern Algeria were characterized by genomic and phenotypic analyses. Isolates were grouped into sixteen different patterns by PCR-RAPD. The phylogenetic status of one representative isolate from each pattern was examined by multilocus sequence analyses of four housekeeping genes (16S rRNA, glnII, recA, and atpD) and one symbiotic gene (nodC). Analysis of 16S rRNA gene sequences showed that all the isolates belonged to the genus Bradyrhizobium. Phylogenetic analyses based on individual or concatenated genes glnII, recA, and atpD indicated that strains cluster in three distinct groups. Ten out of the sixteen strains grouped together with Bradyrhizobium japonicum, while a second group of four clustered with Bradyrhizobium canariense. The third group, represented by isolates CTS8 and CTS57, differed significantly from all other bradyrhizobia known to nodulate members of the Genisteae tribe. In contrast with core genes, sequences of the nodC symbiotic gene from all the examined strains form a homogeneous group within the genistearum symbiovar of Bradyrhizobium. All strains tested nodulated Lupinus angustifolius, Lupinus luteus, and Spartium junceum but not Glycine max. From these results, it is concluded that C. villosus CTS8 and CTS57 strains represent a new lineage within the Bradyrhizobium genus. |
| doi_str_mv | 10.1007/s10482-014-0173-9 |
| format | Article |
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Isolates were grouped into sixteen different patterns by PCR-RAPD. The phylogenetic status of one representative isolate from each pattern was examined by multilocus sequence analyses of four housekeeping genes (16S rRNA, glnII, recA, and atpD) and one symbiotic gene (nodC). Analysis of 16S rRNA gene sequences showed that all the isolates belonged to the genus Bradyrhizobium. Phylogenetic analyses based on individual or concatenated genes glnII, recA, and atpD indicated that strains cluster in three distinct groups. Ten out of the sixteen strains grouped together with Bradyrhizobium japonicum, while a second group of four clustered with Bradyrhizobium canariense. The third group, represented by isolates CTS8 and CTS57, differed significantly from all other bradyrhizobia known to nodulate members of the Genisteae tribe. In contrast with core genes, sequences of the nodC symbiotic gene from all the examined strains form a homogeneous group within the genistearum symbiovar of Bradyrhizobium. All strains tested nodulated Lupinus angustifolius, Lupinus luteus, and Spartium junceum but not Glycine max. From these results, it is concluded that C. villosus CTS8 and CTS57 strains represent a new lineage within the Bradyrhizobium genus.</description><identifier>ISSN: 0003-6072</identifier><identifier>EISSN: 1572-9699</identifier><identifier>DOI: 10.1007/s10482-014-0173-9</identifier><identifier>PMID: 24756908</identifier><language>eng</language><publisher>Cham: Springer-Verlag</publisher><subject>Algeria ; Bacterial Proteins - genetics ; Biomedical and Life Sciences ; Bradyrhizobium ; Bradyrhizobium - classification ; Bradyrhizobium - genetics ; Bradyrhizobium - isolation & purification ; Bradyrhizobium japonicum ; Cluster Analysis ; Cytisus ; Cytisus - microbiology ; Cytisus - physiology ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; essential genes ; Genes, Bacterial ; Genisteae ; Genomics ; Genotype & phenotype ; Glycine max ; Life Sciences ; Lupinus - microbiology ; Lupinus - physiology ; Lupinus angustifolius ; Lupinus luteus ; Medical Microbiology ; Microbiology ; Molecular Sequence Data ; Multilocus Sequence Typing ; nucleotide sequences ; Original Paper ; Phylogenetics ; Phylogeny ; Plant Root Nodulation ; Plant Sciences ; Polymerase Chain Reaction ; Random Amplified Polymorphic DNA Technique ; Ribonucleic acid ; ribosomal RNA ; RNA ; RNA, Ribosomal, 16S - genetics ; root nodules ; sequence analysis ; Soil Science & Conservation ; Spartium - microbiology ; Spartium - physiology ; Spartium junceum</subject><ispartof>Antonie van Leeuwenhoek, 2014-06, Vol.105 (6), p.1121-1129</ispartof><rights>Springer International Publishing Switzerland 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-86ae0d62ee41d214debc2ccfdd012f8e1937d4ecf4607b18074061711e0de7e53</citedby><cites>FETCH-LOGICAL-c429t-86ae0d62ee41d214debc2ccfdd012f8e1937d4ecf4607b18074061711e0de7e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10482-014-0173-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10482-014-0173-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24756908$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ahnia, Hadjira</creatorcontrib><creatorcontrib>Boulila, Farida</creatorcontrib><creatorcontrib>Boulila, Abdelghani</creatorcontrib><creatorcontrib>Boucheffa, Karima</creatorcontrib><creatorcontrib>Durán, David</creatorcontrib><creatorcontrib>Bourebaba, Yasmina</creatorcontrib><creatorcontrib>Salmi, Adouda</creatorcontrib><creatorcontrib>Imperial, Juan</creatorcontrib><creatorcontrib>Ruiz-Argüeso, Tomás</creatorcontrib><creatorcontrib>Rey, Luis</creatorcontrib><title>Cytisus villosus from Northeastern Algeria is nodulated by genetically diverse Bradyrhizobium strains</title><title>Antonie van Leeuwenhoek</title><addtitle>Antonie van Leeuwenhoek</addtitle><addtitle>Antonie Van Leeuwenhoek</addtitle><description>Fifty-one rhizobial strains isolated from root nodules of Cytisus villosus growing in Northeastern Algeria were characterized by genomic and phenotypic analyses. Isolates were grouped into sixteen different patterns by PCR-RAPD. The phylogenetic status of one representative isolate from each pattern was examined by multilocus sequence analyses of four housekeeping genes (16S rRNA, glnII, recA, and atpD) and one symbiotic gene (nodC). Analysis of 16S rRNA gene sequences showed that all the isolates belonged to the genus Bradyrhizobium. Phylogenetic analyses based on individual or concatenated genes glnII, recA, and atpD indicated that strains cluster in three distinct groups. Ten out of the sixteen strains grouped together with Bradyrhizobium japonicum, while a second group of four clustered with Bradyrhizobium canariense. The third group, represented by isolates CTS8 and CTS57, differed significantly from all other bradyrhizobia known to nodulate members of the Genisteae tribe. In contrast with core genes, sequences of the nodC symbiotic gene from all the examined strains form a homogeneous group within the genistearum symbiovar of Bradyrhizobium. All strains tested nodulated Lupinus angustifolius, Lupinus luteus, and Spartium junceum but not Glycine max. From these results, it is concluded that C. villosus CTS8 and CTS57 strains represent a new lineage within the Bradyrhizobium genus.</description><subject>Algeria</subject><subject>Bacterial Proteins - genetics</subject><subject>Biomedical and Life Sciences</subject><subject>Bradyrhizobium</subject><subject>Bradyrhizobium - classification</subject><subject>Bradyrhizobium - genetics</subject><subject>Bradyrhizobium - isolation & purification</subject><subject>Bradyrhizobium japonicum</subject><subject>Cluster Analysis</subject><subject>Cytisus</subject><subject>Cytisus - microbiology</subject><subject>Cytisus - physiology</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>essential genes</subject><subject>Genes, Bacterial</subject><subject>Genisteae</subject><subject>Genomics</subject><subject>Genotype & phenotype</subject><subject>Glycine max</subject><subject>Life Sciences</subject><subject>Lupinus - microbiology</subject><subject>Lupinus - physiology</subject><subject>Lupinus angustifolius</subject><subject>Lupinus luteus</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Multilocus Sequence Typing</subject><subject>nucleotide sequences</subject><subject>Original Paper</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Plant Root Nodulation</subject><subject>Plant Sciences</subject><subject>Polymerase Chain Reaction</subject><subject>Random Amplified Polymorphic DNA Technique</subject><subject>Ribonucleic acid</subject><subject>ribosomal RNA</subject><subject>RNA</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>root nodules</subject><subject>sequence analysis</subject><subject>Soil Science & Conservation</subject><subject>Spartium - microbiology</subject><subject>Spartium - physiology</subject><subject>Spartium junceum</subject><issn>0003-6072</issn><issn>1572-9699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNksFu1TAQRS1ERV8LH8AGLLHpJsV2nDhelqcWkCpYQNeWE09eXTlx8SSVwtfjkIIQC8TCskc-947G14S85OycM6beImeyEQXjMi9VFvoJ2fFKiULXWj8lO8ZYWdRMiWNygniXS1036hk5FlJVtWbNjsB-mTzOSB98CHE99CkO9FNM0y1YnCCN9CIcIHlLPdIxujnYCRxtF3qAESbf2RAW6vwDJAT6Llm3pFv_PbZ-HihOyfoRn5Oj3gaEF4_7Kbm5uvy6_1Bcf37_cX9xXXRS6KloagvM1QJAcie4dNB2out65xgXfQNcl8pJ6HqZh2p5w5RkNVecZxUoqMpTcrb53qf4bQaczOCxgxDsCHFGw6syP5jOzf4DFRkVkjcZffMXehfnNOZBflKi4qxcDflGdSkiJujNffKDTYvhzKxxmS0uk-Mya1xm1bx6dJ7bAdxvxa98MiA2APPVmGP4o_U_XF9vot5GYw_Jo7n5IjKQP0BT1UqVPwBCa6nU</recordid><startdate>20140601</startdate><enddate>20140601</enddate><creator>Ahnia, Hadjira</creator><creator>Boulila, Farida</creator><creator>Boulila, Abdelghani</creator><creator>Boucheffa, Karima</creator><creator>Durán, David</creator><creator>Bourebaba, Yasmina</creator><creator>Salmi, Adouda</creator><creator>Imperial, Juan</creator><creator>Ruiz-Argüeso, Tomás</creator><creator>Rey, Luis</creator><general>Springer-Verlag</general><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>RC3</scope></search><sort><creationdate>20140601</creationdate><title>Cytisus villosus from Northeastern Algeria is nodulated by genetically diverse Bradyrhizobium strains</title><author>Ahnia, Hadjira ; Boulila, Farida ; Boulila, Abdelghani ; Boucheffa, Karima ; Durán, David ; Bourebaba, Yasmina ; Salmi, Adouda ; Imperial, Juan ; Ruiz-Argüeso, Tomás ; Rey, Luis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-86ae0d62ee41d214debc2ccfdd012f8e1937d4ecf4607b18074061711e0de7e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Algeria</topic><topic>Bacterial Proteins - genetics</topic><topic>Biomedical and Life Sciences</topic><topic>Bradyrhizobium</topic><topic>Bradyrhizobium - classification</topic><topic>Bradyrhizobium - genetics</topic><topic>Bradyrhizobium - isolation & purification</topic><topic>Bradyrhizobium japonicum</topic><topic>Cluster Analysis</topic><topic>Cytisus</topic><topic>Cytisus - microbiology</topic><topic>Cytisus - physiology</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>essential genes</topic><topic>Genes, Bacterial</topic><topic>Genisteae</topic><topic>Genomics</topic><topic>Genotype & phenotype</topic><topic>Glycine max</topic><topic>Life Sciences</topic><topic>Lupinus - microbiology</topic><topic>Lupinus - physiology</topic><topic>Lupinus angustifolius</topic><topic>Lupinus luteus</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Multilocus Sequence Typing</topic><topic>nucleotide sequences</topic><topic>Original Paper</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Plant Root Nodulation</topic><topic>Plant Sciences</topic><topic>Polymerase Chain Reaction</topic><topic>Random Amplified Polymorphic DNA Technique</topic><topic>Ribonucleic acid</topic><topic>ribosomal RNA</topic><topic>RNA</topic><topic>RNA, Ribosomal, 16S - 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Academic</collection><collection>Genetics Abstracts</collection><jtitle>Antonie van Leeuwenhoek</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ahnia, Hadjira</au><au>Boulila, Farida</au><au>Boulila, Abdelghani</au><au>Boucheffa, Karima</au><au>Durán, David</au><au>Bourebaba, Yasmina</au><au>Salmi, Adouda</au><au>Imperial, Juan</au><au>Ruiz-Argüeso, Tomás</au><au>Rey, Luis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytisus villosus from Northeastern Algeria is nodulated by genetically diverse Bradyrhizobium strains</atitle><jtitle>Antonie van Leeuwenhoek</jtitle><stitle>Antonie van Leeuwenhoek</stitle><addtitle>Antonie Van Leeuwenhoek</addtitle><date>2014-06-01</date><risdate>2014</risdate><volume>105</volume><issue>6</issue><spage>1121</spage><epage>1129</epage><pages>1121-1129</pages><issn>0003-6072</issn><eissn>1572-9699</eissn><abstract>Fifty-one rhizobial strains isolated from root nodules of Cytisus villosus growing in Northeastern Algeria were characterized by genomic and phenotypic analyses. Isolates were grouped into sixteen different patterns by PCR-RAPD. The phylogenetic status of one representative isolate from each pattern was examined by multilocus sequence analyses of four housekeeping genes (16S rRNA, glnII, recA, and atpD) and one symbiotic gene (nodC). Analysis of 16S rRNA gene sequences showed that all the isolates belonged to the genus Bradyrhizobium. Phylogenetic analyses based on individual or concatenated genes glnII, recA, and atpD indicated that strains cluster in three distinct groups. Ten out of the sixteen strains grouped together with Bradyrhizobium japonicum, while a second group of four clustered with Bradyrhizobium canariense. The third group, represented by isolates CTS8 and CTS57, differed significantly from all other bradyrhizobia known to nodulate members of the Genisteae tribe. In contrast with core genes, sequences of the nodC symbiotic gene from all the examined strains form a homogeneous group within the genistearum symbiovar of Bradyrhizobium. All strains tested nodulated Lupinus angustifolius, Lupinus luteus, and Spartium junceum but not Glycine max. From these results, it is concluded that C. villosus CTS8 and CTS57 strains represent a new lineage within the Bradyrhizobium genus.</abstract><cop>Cham</cop><pub>Springer-Verlag</pub><pmid>24756908</pmid><doi>10.1007/s10482-014-0173-9</doi><tpages>9</tpages></addata></record> |
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| ispartof | Antonie van Leeuwenhoek, 2014-06, Vol.105 (6), p.1121-1129 |
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| subjects | Algeria Bacterial Proteins - genetics Biomedical and Life Sciences Bradyrhizobium Bradyrhizobium - classification Bradyrhizobium - genetics Bradyrhizobium - isolation & purification Bradyrhizobium japonicum Cluster Analysis Cytisus Cytisus - microbiology Cytisus - physiology DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics essential genes Genes, Bacterial Genisteae Genomics Genotype & phenotype Glycine max Life Sciences Lupinus - microbiology Lupinus - physiology Lupinus angustifolius Lupinus luteus Medical Microbiology Microbiology Molecular Sequence Data Multilocus Sequence Typing nucleotide sequences Original Paper Phylogenetics Phylogeny Plant Root Nodulation Plant Sciences Polymerase Chain Reaction Random Amplified Polymorphic DNA Technique Ribonucleic acid ribosomal RNA RNA RNA, Ribosomal, 16S - genetics root nodules sequence analysis Soil Science & Conservation Spartium - microbiology Spartium - physiology Spartium junceum |
| title | Cytisus villosus from Northeastern Algeria is nodulated by genetically diverse Bradyrhizobium strains |
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