α-Glucosidase Inhibition and Antioxidant Properties of Streptomyces sp.: In Vitro
Streptomyces strain isolated from the soil sediment was studied for its in vitro α-glucosidase and antioxidant properties. Morphological characterization and 16S rRNA partial gene sequencing were carried out to confirm that the strain Loyola AR1 belongs to genus Streptomyces sp. Modified nutrient gl...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2014-02, Vol.172 (3), p.1687-1698 |
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| creator | Praveen Kumar, P Preetam Raj, J. P Nimal Christhudas, I. V. S Sagaya Jansi, R Narbert Raj, M Agastian, P |
| description | Streptomyces strain isolated from the soil sediment was studied for its in vitro α-glucosidase and antioxidant properties. Morphological characterization and 16S rRNA partial gene sequencing were carried out to confirm that the strain Loyola AR1 belongs to genus Streptomyces sp. Modified nutrient glucose broth was used as the basal medium for growth and metabolites production. Ethyl acetate extract of Loyola AR1 (EA-Loyola AR1) showed 50 % α-glucosidase inhibition at the concentration of 860.50 ± 2.68 μg/ml. Antioxidant properties such as total phenolic content of EA-Loyola AR1 was 176.83 ± 1.17 mg of catechol equivalents/g extracts. EA-Loyola AR1 showed significant scavenging activity on 2,2-diphenyl-picrylhydrazyl (50 % inhibition (IC₅₀), 750.50 ± 1.61 μg/ml), hydroxyl (IC₅₀, 690.20 ± 2.38 μg/ml), nitric oxide (IC₅₀, 850.50 ± 1.77 μg/ml), and superoxide (IC₅₀, 880.08 ± 1.80 μg/ml) radicals, as well as reducing power. EA-Loyola AR1 showed strong suppressive effect on lipid peroxidation (IC₅₀, 670.50 ± 2.52 μg/ml). Antioxidants of β-carotene linoleate model system reveals significantly lower than butylated hydroxyanisole. |
| doi_str_mv | 10.1007/s12010-013-0650-z |
| format | Article |
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P ; Nimal Christhudas, I. V. S ; Sagaya Jansi, R ; Narbert Raj, M ; Agastian, P</creator><creatorcontrib>Praveen Kumar, P ; Preetam Raj, J. P ; Nimal Christhudas, I. V. S ; Sagaya Jansi, R ; Narbert Raj, M ; Agastian, P</creatorcontrib><description>Streptomyces strain isolated from the soil sediment was studied for its in vitro α-glucosidase and antioxidant properties. Morphological characterization and 16S rRNA partial gene sequencing were carried out to confirm that the strain Loyola AR1 belongs to genus Streptomyces sp. Modified nutrient glucose broth was used as the basal medium for growth and metabolites production. Ethyl acetate extract of Loyola AR1 (EA-Loyola AR1) showed 50 % α-glucosidase inhibition at the concentration of 860.50 ± 2.68 μg/ml. Antioxidant properties such as total phenolic content of EA-Loyola AR1 was 176.83 ± 1.17 mg of catechol equivalents/g extracts. EA-Loyola AR1 showed significant scavenging activity on 2,2-diphenyl-picrylhydrazyl (50 % inhibition (IC₅₀), 750.50 ± 1.61 μg/ml), hydroxyl (IC₅₀, 690.20 ± 2.38 μg/ml), nitric oxide (IC₅₀, 850.50 ± 1.77 μg/ml), and superoxide (IC₅₀, 880.08 ± 1.80 μg/ml) radicals, as well as reducing power. EA-Loyola AR1 showed strong suppressive effect on lipid peroxidation (IC₅₀, 670.50 ± 2.52 μg/ml). Antioxidants of β-carotene linoleate model system reveals significantly lower than butylated hydroxyanisole.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-013-0650-z</identifier><identifier>PMID: 24242165</identifier><identifier>CODEN: ABIBDL</identifier><language>eng</language><publisher>Boston: Springer-Verlag</publisher><subject>acetates ; alpha-glucosidase ; alpha-Glucosidases - chemistry ; antioxidant activity ; antioxidants ; Antioxidants - chemistry ; Antioxidants - pharmacology ; beta-carotene ; Biochemistry ; Biological and medical sciences ; Biotechnology ; butylated hydroxyanisole ; catechol ; Chemistry ; Chemistry and Materials Science ; culture media ; Enzyme Inhibitors - chemistry ; Enzyme Inhibitors - pharmacology ; Free Radical Scavengers - chemistry ; Free Radical Scavengers - pharmacology ; free radicals ; Fundamental and applied biological sciences. Psychology ; genes ; glucose ; Glycoside Hydrolase Inhibitors ; Hydroxyl Radical - chemistry ; Hydroxyl Radical - pharmacology ; inhibitory concentration 50 ; lipid peroxidation ; Lipid Peroxidation - drug effects ; metabolites ; nitric oxide ; Phenols - chemistry ; Phenols - pharmacology ; ribosomal RNA ; RNA, Ribosomal, 16S - genetics ; sediments ; soil ; Streptomyces ; Streptomyces - chemistry ; Streptomyces - enzymology ; Streptomyces - genetics</subject><ispartof>Applied biochemistry and biotechnology, 2014-02, Vol.172 (3), p.1687-1698</ispartof><rights>Springer Science+Business Media New York 2013</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-e0667c955ddf558373fb8e65dfea7788c96b48c333e48e128f802414d3f134093</citedby><cites>FETCH-LOGICAL-c431t-e0667c955ddf558373fb8e65dfea7788c96b48c333e48e128f802414d3f134093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-013-0650-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-013-0650-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28562509$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24242165$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Praveen Kumar, P</creatorcontrib><creatorcontrib>Preetam Raj, J. P</creatorcontrib><creatorcontrib>Nimal Christhudas, I. V. S</creatorcontrib><creatorcontrib>Sagaya Jansi, R</creatorcontrib><creatorcontrib>Narbert Raj, M</creatorcontrib><creatorcontrib>Agastian, P</creatorcontrib><title>α-Glucosidase Inhibition and Antioxidant Properties of Streptomyces sp.: In Vitro</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>Streptomyces strain isolated from the soil sediment was studied for its in vitro α-glucosidase and antioxidant properties. Morphological characterization and 16S rRNA partial gene sequencing were carried out to confirm that the strain Loyola AR1 belongs to genus Streptomyces sp. Modified nutrient glucose broth was used as the basal medium for growth and metabolites production. Ethyl acetate extract of Loyola AR1 (EA-Loyola AR1) showed 50 % α-glucosidase inhibition at the concentration of 860.50 ± 2.68 μg/ml. Antioxidant properties such as total phenolic content of EA-Loyola AR1 was 176.83 ± 1.17 mg of catechol equivalents/g extracts. EA-Loyola AR1 showed significant scavenging activity on 2,2-diphenyl-picrylhydrazyl (50 % inhibition (IC₅₀), 750.50 ± 1.61 μg/ml), hydroxyl (IC₅₀, 690.20 ± 2.38 μg/ml), nitric oxide (IC₅₀, 850.50 ± 1.77 μg/ml), and superoxide (IC₅₀, 880.08 ± 1.80 μg/ml) radicals, as well as reducing power. EA-Loyola AR1 showed strong suppressive effect on lipid peroxidation (IC₅₀, 670.50 ± 2.52 μg/ml). Antioxidants of β-carotene linoleate model system reveals significantly lower than butylated hydroxyanisole.</description><subject>acetates</subject><subject>alpha-glucosidase</subject><subject>alpha-Glucosidases - chemistry</subject><subject>antioxidant activity</subject><subject>antioxidants</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - pharmacology</subject><subject>beta-carotene</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>butylated hydroxyanisole</subject><subject>catechol</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>culture media</subject><subject>Enzyme Inhibitors - chemistry</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Free Radical Scavengers - chemistry</subject><subject>Free Radical Scavengers - pharmacology</subject><subject>free radicals</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>glucose</subject><subject>Glycoside Hydrolase Inhibitors</subject><subject>Hydroxyl Radical - chemistry</subject><subject>Hydroxyl Radical - pharmacology</subject><subject>inhibitory concentration 50</subject><subject>lipid peroxidation</subject><subject>Lipid Peroxidation - drug effects</subject><subject>metabolites</subject><subject>nitric oxide</subject><subject>Phenols - chemistry</subject><subject>Phenols - pharmacology</subject><subject>ribosomal RNA</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>sediments</subject><subject>soil</subject><subject>Streptomyces</subject><subject>Streptomyces - chemistry</subject><subject>Streptomyces - enzymology</subject><subject>Streptomyces - genetics</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM9OFTEUxhsikevVB2AjszFxUzj9N9NxR4giCQlExG3T22mxZG47tjOJ8Fa-CM9EL3PBnTFdnDbn932n50Non8AhAWiOMqFAAANhGGoB-H4HLYgQLQbakldoAbRhmFLZ7qE3Od8CECpF8xrtUV4OqcUCfXv4g0_7ycTsO51tdRZ--pUffQyVDl11HMr1d2mFsbpMcbBp9DZX0VVXY7LDGNd3przzcPipSKsffkzxLdp1us_23bYu0fWXz99PvuLzi9Ozk-NzbDgjI7ZQ141pheg6J4RkDXMraWvROaubRkrT1isuDWPMcmnLz50EygnvmCOMQ8uW6OPsO6T4a7J5VGufje17HWycsiKCcVkWbsV_oMCEaDZliciMmhRzTtapIfm1TneKgNqkrubUVUldbVJX90Xzfms_rda2e1E8x1yAD1tAZ6N7l3QwPv_lpKipeFqJzlwurXBjk7qNUwolxX9OP5hFTkelb1Ixvr4qEAcAQSWv2SPIwaJ-</recordid><startdate>20140201</startdate><enddate>20140201</enddate><creator>Praveen Kumar, P</creator><creator>Preetam Raj, J. 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S ; Sagaya Jansi, R ; Narbert Raj, M ; Agastian, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-e0667c955ddf558373fb8e65dfea7788c96b48c333e48e128f802414d3f134093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>acetates</topic><topic>alpha-glucosidase</topic><topic>alpha-Glucosidases - chemistry</topic><topic>antioxidant activity</topic><topic>antioxidants</topic><topic>Antioxidants - chemistry</topic><topic>Antioxidants - pharmacology</topic><topic>beta-carotene</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>butylated hydroxyanisole</topic><topic>catechol</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>culture media</topic><topic>Enzyme Inhibitors - chemistry</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Free Radical Scavengers - chemistry</topic><topic>Free Radical Scavengers - pharmacology</topic><topic>free radicals</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>glucose</topic><topic>Glycoside Hydrolase Inhibitors</topic><topic>Hydroxyl Radical - chemistry</topic><topic>Hydroxyl Radical - pharmacology</topic><topic>inhibitory concentration 50</topic><topic>lipid peroxidation</topic><topic>Lipid Peroxidation - drug effects</topic><topic>metabolites</topic><topic>nitric oxide</topic><topic>Phenols - chemistry</topic><topic>Phenols - pharmacology</topic><topic>ribosomal RNA</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>sediments</topic><topic>soil</topic><topic>Streptomyces</topic><topic>Streptomyces - chemistry</topic><topic>Streptomyces - enzymology</topic><topic>Streptomyces - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Praveen Kumar, P</creatorcontrib><creatorcontrib>Preetam Raj, J. P</creatorcontrib><creatorcontrib>Nimal Christhudas, I. V. 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P</au><au>Nimal Christhudas, I. V. S</au><au>Sagaya Jansi, R</au><au>Narbert Raj, M</au><au>Agastian, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>α-Glucosidase Inhibition and Antioxidant Properties of Streptomyces sp.: In Vitro</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2014-02-01</date><risdate>2014</risdate><volume>172</volume><issue>3</issue><spage>1687</spage><epage>1698</epage><pages>1687-1698</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><coden>ABIBDL</coden><abstract>Streptomyces strain isolated from the soil sediment was studied for its in vitro α-glucosidase and antioxidant properties. Morphological characterization and 16S rRNA partial gene sequencing were carried out to confirm that the strain Loyola AR1 belongs to genus Streptomyces sp. Modified nutrient glucose broth was used as the basal medium for growth and metabolites production. Ethyl acetate extract of Loyola AR1 (EA-Loyola AR1) showed 50 % α-glucosidase inhibition at the concentration of 860.50 ± 2.68 μg/ml. Antioxidant properties such as total phenolic content of EA-Loyola AR1 was 176.83 ± 1.17 mg of catechol equivalents/g extracts. EA-Loyola AR1 showed significant scavenging activity on 2,2-diphenyl-picrylhydrazyl (50 % inhibition (IC₅₀), 750.50 ± 1.61 μg/ml), hydroxyl (IC₅₀, 690.20 ± 2.38 μg/ml), nitric oxide (IC₅₀, 850.50 ± 1.77 μg/ml), and superoxide (IC₅₀, 880.08 ± 1.80 μg/ml) radicals, as well as reducing power. EA-Loyola AR1 showed strong suppressive effect on lipid peroxidation (IC₅₀, 670.50 ± 2.52 μg/ml). Antioxidants of β-carotene linoleate model system reveals significantly lower than butylated hydroxyanisole.</abstract><cop>Boston</cop><pub>Springer-Verlag</pub><pmid>24242165</pmid><doi>10.1007/s12010-013-0650-z</doi><tpages>12</tpages></addata></record> |
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| subjects | acetates alpha-glucosidase alpha-Glucosidases - chemistry antioxidant activity antioxidants Antioxidants - chemistry Antioxidants - pharmacology beta-carotene Biochemistry Biological and medical sciences Biotechnology butylated hydroxyanisole catechol Chemistry Chemistry and Materials Science culture media Enzyme Inhibitors - chemistry Enzyme Inhibitors - pharmacology Free Radical Scavengers - chemistry Free Radical Scavengers - pharmacology free radicals Fundamental and applied biological sciences. Psychology genes glucose Glycoside Hydrolase Inhibitors Hydroxyl Radical - chemistry Hydroxyl Radical - pharmacology inhibitory concentration 50 lipid peroxidation Lipid Peroxidation - drug effects metabolites nitric oxide Phenols - chemistry Phenols - pharmacology ribosomal RNA RNA, Ribosomal, 16S - genetics sediments soil Streptomyces Streptomyces - chemistry Streptomyces - enzymology Streptomyces - genetics |
| title | α-Glucosidase Inhibition and Antioxidant Properties of Streptomyces sp.: In Vitro |
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