Probing the RNA Binding Surface of the HIV‑1 Nucleocapsid Protein by Site-Directed Mutagenesis

The highly conserved nucleocapsid protein domain in HIV-1 recognizes and binds SL3 in genomic RNA. In this work, we used the structure of the NCp7–SL3 RNA complex to guide the construction of 16 NCp7 mutants to probe the RNA binding surface of the protein [De Guzman, R. N., et al. (1998) Science 279...

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Veröffentlicht in:	Biochemistry (Easton) 2013-05, Vol.52 (19), p.3358-3368
Hauptverfasser:	Ouyang, Wei, Okaine, Stephen, McPike, Mark P, Lin, Yong, Borer, Philip N
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino Acid Substitution Base Sequence gag Gene Products, Human Immunodeficiency Virus - chemistry gag Gene Products, Human Immunodeficiency Virus - genetics gag Gene Products, Human Immunodeficiency Virus - metabolism Genome, Viral HIV-1 - genetics HIV-1 - metabolism Human immunodeficiency virus 1 Humans Kinetics Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed Protein Binding Protein Conformation RNA, Spliced Leader - genetics RNA, Spliced Leader - metabolism RNA, Viral - genetics RNA, Viral - metabolism
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