Early gene expression programs accompanying trans-differentiation of epidermal cells of Vicia faba cotyledons into transfer cells
Transfer cells (TCs) trans-differentiate from differentiated cells by developing extensive wall ingrowths that enhance plasma membrane transport of nutrients. Here, we investigated transcriptional changes accompanying induction of TC development in adaxial epidermal cells of cultured Vicia faba coty...
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| Veröffentlicht in: | The New phytologist 2009-06, Vol.182 (4), p.863-877 |
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| creator | Dibley, Stephen J Zhou, Yuchan Andriunas, Felicity A Talbot, Mark J Offler, Christina E Patrick, John W McCurdy, David W |
| description | Transfer cells (TCs) trans-differentiate from differentiated cells by developing extensive wall ingrowths that enhance plasma membrane transport of nutrients. Here, we investigated transcriptional changes accompanying induction of TC development in adaxial epidermal cells of cultured Vicia faba cotyledons. Global changes in gene expression revealed by cDNA-AFLP were compared between adaxial epidermal cells during induction (3 h) and subsequent building (24 h) of wall ingrowths, and in cells of adjoining storage parenchyma tissue, which do not form wall ingrowths. A total of 5795 transcript-derived fragments (TDFs) were detected; of these, 264 TDFs showed epidermal-specific changes in gene expression and a further 207 TDFs were differentially expressed in both epidermal and storage parenchyma cells. Genes involved in signalling (auxin/ethylene), metabolism (mitochondrial; storage product hydrolysis), cell division, vesicle trafficking and cell wall biosynthesis were specifically induced in epidermal TCs. Blockers of auxin action and vesicle trafficking inhibited ingrowth formation and marked increases in cell division accompanied TC development. Auxin and possibly ethylene signalling cascades induce epidermal cells of V. faba cotyledons to trans-differentiate into TCs. Trans-differentiation is initiated by rapid de-differentiation to a mitotic state accompanied by mitochondrial biogenesis driving storage product hydrolysis to fuel wall ingrowth formation orchestrated by a modified vesicle trafficking mechanism. |
| doi_str_mv | 10.1111/j.1469-8137.2009.02822.x |
| format | Article |
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Here, we investigated transcriptional changes accompanying induction of TC development in adaxial epidermal cells of cultured Vicia faba cotyledons. Global changes in gene expression revealed by cDNA-AFLP were compared between adaxial epidermal cells during induction (3 h) and subsequent building (24 h) of wall ingrowths, and in cells of adjoining storage parenchyma tissue, which do not form wall ingrowths. A total of 5795 transcript-derived fragments (TDFs) were detected; of these, 264 TDFs showed epidermal-specific changes in gene expression and a further 207 TDFs were differentially expressed in both epidermal and storage parenchyma cells. Genes involved in signalling (auxin/ethylene), metabolism (mitochondrial; storage product hydrolysis), cell division, vesicle trafficking and cell wall biosynthesis were specifically induced in epidermal TCs. Blockers of auxin action and vesicle trafficking inhibited ingrowth formation and marked increases in cell division accompanied TC development. Auxin and possibly ethylene signalling cascades induce epidermal cells of V. faba cotyledons to trans-differentiate into TCs. Trans-differentiation is initiated by rapid de-differentiation to a mitotic state accompanied by mitochondrial biogenesis driving storage product hydrolysis to fuel wall ingrowth formation orchestrated by a modified vesicle trafficking mechanism.</description><identifier>ISSN: 0028-646X</identifier><identifier>EISSN: 1469-8137</identifier><identifier>DOI: 10.1111/j.1469-8137.2009.02822.x</identifier><identifier>PMID: 19383101</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Amplified Fragment Length Polymorphism Analysis ; cDNA-AFLP ; Cell Division - drug effects ; Cell Transdifferentiation - drug effects ; Cell Transdifferentiation - genetics ; Cell Wall - drug effects ; Cell Wall - metabolism ; Cells, Cultured ; Cotyledon - cytology ; Cotyledon - drug effects ; Cotyledon - genetics ; Cotyledon - ultrastructure ; DNA, Complementary - genetics ; Ethylenes - pharmacology ; Gene Expression Profiling ; Gene Expression Regulation, Plant - drug effects ; Genes, Plant - genetics ; Indoleacetic Acids - pharmacology ; Organ Specificity - drug effects ; Organ Specificity - genetics ; Plant Epidermis - cytology ; Plant Epidermis - drug effects ; Plant Epidermis - genetics ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Protein Biosynthesis - drug effects ; RNA, Plant - isolation & purification ; trans-differentiation ; Transcription, Genetic - drug effects ; transfer cells ; Vicia faba ; Vicia faba - cytology ; Vicia faba - drug effects ; Vicia faba - genetics ; Vicia faba - ultrastructure ; wall ingrowths</subject><ispartof>The New phytologist, 2009-06, Vol.182 (4), p.863-877</ispartof><rights>The Authors (2009). 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Here, we investigated transcriptional changes accompanying induction of TC development in adaxial epidermal cells of cultured Vicia faba cotyledons. Global changes in gene expression revealed by cDNA-AFLP were compared between adaxial epidermal cells during induction (3 h) and subsequent building (24 h) of wall ingrowths, and in cells of adjoining storage parenchyma tissue, which do not form wall ingrowths. A total of 5795 transcript-derived fragments (TDFs) were detected; of these, 264 TDFs showed epidermal-specific changes in gene expression and a further 207 TDFs were differentially expressed in both epidermal and storage parenchyma cells. Genes involved in signalling (auxin/ethylene), metabolism (mitochondrial; storage product hydrolysis), cell division, vesicle trafficking and cell wall biosynthesis were specifically induced in epidermal TCs. Blockers of auxin action and vesicle trafficking inhibited ingrowth formation and marked increases in cell division accompanied TC development. Auxin and possibly ethylene signalling cascades induce epidermal cells of V. faba cotyledons to trans-differentiate into TCs. Trans-differentiation is initiated by rapid de-differentiation to a mitotic state accompanied by mitochondrial biogenesis driving storage product hydrolysis to fuel wall ingrowth formation orchestrated by a modified vesicle trafficking mechanism.</description><subject>Amplified Fragment Length Polymorphism Analysis</subject><subject>cDNA-AFLP</subject><subject>Cell Division - drug effects</subject><subject>Cell Transdifferentiation - drug effects</subject><subject>Cell Transdifferentiation - genetics</subject><subject>Cell Wall - drug effects</subject><subject>Cell Wall - metabolism</subject><subject>Cells, Cultured</subject><subject>Cotyledon - cytology</subject><subject>Cotyledon - drug effects</subject><subject>Cotyledon - genetics</subject><subject>Cotyledon - ultrastructure</subject><subject>DNA, Complementary - genetics</subject><subject>Ethylenes - pharmacology</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Genes, Plant - genetics</subject><subject>Indoleacetic Acids - pharmacology</subject><subject>Organ Specificity - drug effects</subject><subject>Organ Specificity - genetics</subject><subject>Plant Epidermis - cytology</subject><subject>Plant Epidermis - drug effects</subject><subject>Plant Epidermis - genetics</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Protein Biosynthesis - drug effects</subject><subject>RNA, Plant - isolation & purification</subject><subject>trans-differentiation</subject><subject>Transcription, Genetic - drug effects</subject><subject>transfer cells</subject><subject>Vicia faba</subject><subject>Vicia faba - cytology</subject><subject>Vicia faba - drug effects</subject><subject>Vicia faba - genetics</subject><subject>Vicia faba - ultrastructure</subject><subject>wall ingrowths</subject><issn>0028-646X</issn><issn>1469-8137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcGP1CAUxonRuOPqv6AcvbQLlKHtwYPZrO4mGzXRNd7IG_qYMGlLhU52evQ_F-xEr3KBPH7f9-B7hFDOSp7W1aHkUrVFw6u6FIy1JRONEOXpCdn8vXhKNiyVCyXVjwvyIsYDS-RWiefkgrdVU3HGN-TXDYR-oXsckeJpChij8yOdgt8HGCIFY_wwwbi4cU_nAGMsOmctBhxnB3NmvaU4uQ7DAD012Pcxl74744Ba2AE1fl567PwYqRtnv9okixV-SZ5Z6CO-Ou-X5OHDzbfr2-L-88e76_f3hZH1VhSi63iLvFYdVla2iskOBLfYoq1bFFbWtlVbIzg0bIcNA1kb1oLiVbUzNTfVJXm7-qa__TxinPXgYn4BjOiPUfNtJXNuSiW0WVETfIwBrZ6CGyAsmjOdB6APOuesM6_zAPSfAehTkr4-dznuBuz-Cc-JJ-DdCjy6Hpf_NtafvtzmU9K_WfUWvIZ9cFE_fBXJmHElatHI6jdVHKBx</recordid><startdate>200906</startdate><enddate>200906</enddate><creator>Dibley, Stephen J</creator><creator>Zhou, Yuchan</creator><creator>Andriunas, Felicity A</creator><creator>Talbot, Mark J</creator><creator>Offler, Christina E</creator><creator>Patrick, John W</creator><creator>McCurdy, David W</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>200906</creationdate><title>Early gene expression programs accompanying trans-differentiation of epidermal cells of Vicia faba cotyledons into transfer cells</title><author>Dibley, Stephen J ; Zhou, Yuchan ; Andriunas, Felicity A ; Talbot, Mark J ; Offler, Christina E ; Patrick, John W ; McCurdy, David W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4752-2dd19e176de3f49604da21fe9ef79e2f47f965c21a80be80a47c09a6133bc71c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Amplified Fragment Length Polymorphism Analysis</topic><topic>cDNA-AFLP</topic><topic>Cell Division - drug effects</topic><topic>Cell Transdifferentiation - drug effects</topic><topic>Cell Transdifferentiation - genetics</topic><topic>Cell Wall - drug effects</topic><topic>Cell Wall - metabolism</topic><topic>Cells, Cultured</topic><topic>Cotyledon - cytology</topic><topic>Cotyledon - drug effects</topic><topic>Cotyledon - genetics</topic><topic>Cotyledon - ultrastructure</topic><topic>DNA, Complementary - genetics</topic><topic>Ethylenes - pharmacology</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Genes, Plant - genetics</topic><topic>Indoleacetic Acids - pharmacology</topic><topic>Organ Specificity - drug effects</topic><topic>Organ Specificity - genetics</topic><topic>Plant Epidermis - cytology</topic><topic>Plant Epidermis - drug effects</topic><topic>Plant Epidermis - genetics</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Protein Biosynthesis - drug effects</topic><topic>RNA, Plant - isolation & purification</topic><topic>trans-differentiation</topic><topic>Transcription, Genetic - drug effects</topic><topic>transfer cells</topic><topic>Vicia faba</topic><topic>Vicia faba - cytology</topic><topic>Vicia faba - drug effects</topic><topic>Vicia faba - genetics</topic><topic>Vicia faba - ultrastructure</topic><topic>wall ingrowths</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dibley, Stephen J</creatorcontrib><creatorcontrib>Zhou, Yuchan</creatorcontrib><creatorcontrib>Andriunas, Felicity A</creatorcontrib><creatorcontrib>Talbot, Mark J</creatorcontrib><creatorcontrib>Offler, Christina E</creatorcontrib><creatorcontrib>Patrick, John W</creatorcontrib><creatorcontrib>McCurdy, David W</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>The New phytologist</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dibley, Stephen J</au><au>Zhou, Yuchan</au><au>Andriunas, Felicity A</au><au>Talbot, Mark J</au><au>Offler, Christina E</au><au>Patrick, John W</au><au>McCurdy, David W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Early gene expression programs accompanying trans-differentiation of epidermal cells of Vicia faba cotyledons into transfer cells</atitle><jtitle>The New phytologist</jtitle><addtitle>New Phytol</addtitle><date>2009-06</date><risdate>2009</risdate><volume>182</volume><issue>4</issue><spage>863</spage><epage>877</epage><pages>863-877</pages><issn>0028-646X</issn><eissn>1469-8137</eissn><abstract>Transfer cells (TCs) trans-differentiate from differentiated cells by developing extensive wall ingrowths that enhance plasma membrane transport of nutrients. Here, we investigated transcriptional changes accompanying induction of TC development in adaxial epidermal cells of cultured Vicia faba cotyledons. Global changes in gene expression revealed by cDNA-AFLP were compared between adaxial epidermal cells during induction (3 h) and subsequent building (24 h) of wall ingrowths, and in cells of adjoining storage parenchyma tissue, which do not form wall ingrowths. A total of 5795 transcript-derived fragments (TDFs) were detected; of these, 264 TDFs showed epidermal-specific changes in gene expression and a further 207 TDFs were differentially expressed in both epidermal and storage parenchyma cells. Genes involved in signalling (auxin/ethylene), metabolism (mitochondrial; storage product hydrolysis), cell division, vesicle trafficking and cell wall biosynthesis were specifically induced in epidermal TCs. Blockers of auxin action and vesicle trafficking inhibited ingrowth formation and marked increases in cell division accompanied TC development. Auxin and possibly ethylene signalling cascades induce epidermal cells of V. faba cotyledons to trans-differentiate into TCs. Trans-differentiation is initiated by rapid de-differentiation to a mitotic state accompanied by mitochondrial biogenesis driving storage product hydrolysis to fuel wall ingrowth formation orchestrated by a modified vesicle trafficking mechanism.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>19383101</pmid><doi>10.1111/j.1469-8137.2009.02822.x</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amplified Fragment Length Polymorphism Analysis cDNA-AFLP Cell Division - drug effects Cell Transdifferentiation - drug effects Cell Transdifferentiation - genetics Cell Wall - drug effects Cell Wall - metabolism Cells, Cultured Cotyledon - cytology Cotyledon - drug effects Cotyledon - genetics Cotyledon - ultrastructure DNA, Complementary - genetics Ethylenes - pharmacology Gene Expression Profiling Gene Expression Regulation, Plant - drug effects Genes, Plant - genetics Indoleacetic Acids - pharmacology Organ Specificity - drug effects Organ Specificity - genetics Plant Epidermis - cytology Plant Epidermis - drug effects Plant Epidermis - genetics Plant Proteins - genetics Plant Proteins - metabolism Protein Biosynthesis - drug effects RNA, Plant - isolation & purification trans-differentiation Transcription, Genetic - drug effects transfer cells Vicia faba Vicia faba - cytology Vicia faba - drug effects Vicia faba - genetics Vicia faba - ultrastructure wall ingrowths |
| title | Early gene expression programs accompanying trans-differentiation of epidermal cells of Vicia faba cotyledons into transfer cells |
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