Identification of an alternative knockdown resistance (kdr)‐like mutation, M918L, and a novel mutation, V1010A, in the Thrips tabaci voltage‐gated sodium channel gene
BACKGROUND: Knockdown resistance (kdr) has been identified as a main mechanism against pyrethroid insecticides in many arthropod pests including in the onion thrips, Thrips tabaci. To characterize and identify pyrethroid‐resistance in onion thrips in Washington state, we conducted insecticide bioass...
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| Veröffentlicht in: | Pest management science 2014-06, Vol.70 (6), p.977-981 |
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| creator | Wu, Meixiang Gotoh, Hiroki Waters, Timothy Walsh, Douglas B Lavine, Laura Corley |
| description | BACKGROUND: Knockdown resistance (kdr) has been identified as a main mechanism against pyrethroid insecticides in many arthropod pests including in the onion thrips, Thrips tabaci. To characterize and identify pyrethroid‐resistance in onion thrips in Washington state, we conducted insecticide bioassays and sequenced a region of the voltage gated sodium channel gene from several different T. tabaci populations. RESULTS: Field collected Thrips tabaci were found to have large variations in resistance to the pyrethroid insecticide lambda‐cyhalothrin. We identified two single nucleotide substitutions in our analysis of a partial sequence of the T. tabaci voltage‐gated sodium channel gene. One mutation resulted in the non‐synonymous substitution of methionine with leucine (M918L), which is well known to be responsible for super knockdown resistance in some pest species. Another non‐synonymous substitution, a valine (GTT) to alanine (GCT) replacement at amino acid 1010 (V1010A) was identified in our study and was associated with lambda‐cyhalothrin resistance. CONCLUSION: We have characterized a known kdr mutation and identified a novel mutation in the voltage‐gated sodium channel gene of Thrips tabaci associated with resistance to lambda‐cyhalothrin. This gene region and these mutations are expected to be useful in the development of a diagnostic test to detect kdr resistance in many onion thrips populations. © 2013 Society of Chemical Industry |
| doi_str_mv | 10.1002/ps.3638 |
| format | Article |
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To characterize and identify pyrethroid‐resistance in onion thrips in Washington state, we conducted insecticide bioassays and sequenced a region of the voltage gated sodium channel gene from several different T. tabaci populations. RESULTS: Field collected Thrips tabaci were found to have large variations in resistance to the pyrethroid insecticide lambda‐cyhalothrin. We identified two single nucleotide substitutions in our analysis of a partial sequence of the T. tabaci voltage‐gated sodium channel gene. One mutation resulted in the non‐synonymous substitution of methionine with leucine (M918L), which is well known to be responsible for super knockdown resistance in some pest species. Another non‐synonymous substitution, a valine (GTT) to alanine (GCT) replacement at amino acid 1010 (V1010A) was identified in our study and was associated with lambda‐cyhalothrin resistance. CONCLUSION: We have characterized a known kdr mutation and identified a novel mutation in the voltage‐gated sodium channel gene of Thrips tabaci associated with resistance to lambda‐cyhalothrin. This gene region and these mutations are expected to be useful in the development of a diagnostic test to detect kdr resistance in many onion thrips populations. © 2013 Society of Chemical Industry</description><identifier>ISSN: 1526-498X</identifier><identifier>EISSN: 1526-4998</identifier><identifier>DOI: 10.1002/ps.3638</identifier><identifier>PMID: 23983138</identifier><identifier>CODEN: PMSCFC</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>alanine ; Allium cepa ; arthropod pests ; Arthropoda ; Arthropods ; Bioassays ; Biological and medical sciences ; Control ; Fundamental and applied biological sciences. Psychology ; Gene Knockdown Techniques ; genes ; Insecticide Resistance - genetics ; Insecticides ; kdr ; lambda-cyhalothrin ; leucine ; methionine ; Mutation ; Nitriles - toxicity ; Pest control ; Phytopathology. Animal pests. Plant and forest protection ; point mutation ; Protozoa. Invertebrates ; Pyrethrins - toxicity ; pyrethroid resistance ; sodium channel ; sodium channels ; Sodium Channels - genetics ; Thrips tabaci ; Thysanoptera - drug effects ; Thysanoptera - genetics ; valine ; Washington</subject><ispartof>Pest management science, 2014-06, Vol.70 (6), p.977-981</ispartof><rights>2013 Society of Chemical Industry</rights><rights>2015 INIST-CNRS</rights><rights>2013 Society of Chemical Industry.</rights><rights>Copyright Wiley Subscription Services, Inc. Jun 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3538-1e07a980aa85ca29a547b72f5381965b1668976d8936aca3c02ec5fe59a194083</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fps.3638$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fps.3638$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28441003$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23983138$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Meixiang</creatorcontrib><creatorcontrib>Gotoh, Hiroki</creatorcontrib><creatorcontrib>Waters, Timothy</creatorcontrib><creatorcontrib>Walsh, Douglas B</creatorcontrib><creatorcontrib>Lavine, Laura Corley</creatorcontrib><title>Identification of an alternative knockdown resistance (kdr)‐like mutation, M918L, and a novel mutation, V1010A, in the Thrips tabaci voltage‐gated sodium channel gene</title><title>Pest management science</title><addtitle>Pest. Manag. Sci</addtitle><description>BACKGROUND: Knockdown resistance (kdr) has been identified as a main mechanism against pyrethroid insecticides in many arthropod pests including in the onion thrips, Thrips tabaci. To characterize and identify pyrethroid‐resistance in onion thrips in Washington state, we conducted insecticide bioassays and sequenced a region of the voltage gated sodium channel gene from several different T. tabaci populations. RESULTS: Field collected Thrips tabaci were found to have large variations in resistance to the pyrethroid insecticide lambda‐cyhalothrin. We identified two single nucleotide substitutions in our analysis of a partial sequence of the T. tabaci voltage‐gated sodium channel gene. One mutation resulted in the non‐synonymous substitution of methionine with leucine (M918L), which is well known to be responsible for super knockdown resistance in some pest species. Another non‐synonymous substitution, a valine (GTT) to alanine (GCT) replacement at amino acid 1010 (V1010A) was identified in our study and was associated with lambda‐cyhalothrin resistance. CONCLUSION: We have characterized a known kdr mutation and identified a novel mutation in the voltage‐gated sodium channel gene of Thrips tabaci associated with resistance to lambda‐cyhalothrin. This gene region and these mutations are expected to be useful in the development of a diagnostic test to detect kdr resistance in many onion thrips populations. © 2013 Society of Chemical Industry</description><subject>alanine</subject><subject>Allium cepa</subject><subject>arthropod pests</subject><subject>Arthropoda</subject><subject>Arthropods</subject><subject>Bioassays</subject><subject>Biological and medical sciences</subject><subject>Control</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Knockdown Techniques</subject><subject>genes</subject><subject>Insecticide Resistance - genetics</subject><subject>Insecticides</subject><subject>kdr</subject><subject>lambda-cyhalothrin</subject><subject>leucine</subject><subject>methionine</subject><subject>Mutation</subject><subject>Nitriles - toxicity</subject><subject>Pest control</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>point mutation</subject><subject>Protozoa. Invertebrates</subject><subject>Pyrethrins - toxicity</subject><subject>pyrethroid resistance</subject><subject>sodium channel</subject><subject>sodium channels</subject><subject>Sodium Channels - genetics</subject><subject>Thrips tabaci</subject><subject>Thysanoptera - drug effects</subject><subject>Thysanoptera - genetics</subject><subject>valine</subject><subject>Washington</subject><issn>1526-498X</issn><issn>1526-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0s1uEzEQAOAVAtESEG8AliqkIpLin_XaPlYVtEWhILUFxMWaeL2Juxs7rHdTeuMReA4eiyfBaUJAnDjZ8nwejT2TZY8JPiAY05eLeMAKJu9ku4TTYpQrJe9u9_LTTvYgxiuMsVKK3s92KFOSESZ3sx-npfWdq5yBzgWPQoXAI2g62_p0srSo9sHUZbj2qLXRxQ68sWi_LtvnP799b1xt0bzvbi8P0VtF5HiYMpQIkA9L2_wV_EAwwYdD5DzqZhZdzFq3iKiDCRiHlqHpYGpTyil0tkQxlK6fIzMD71OWqfX2YXavgibaR5t1kF2-fnVxdDIavzs-PTocjwzjTI6IxQKUxACSG6AKeC4mglYpRlTBJ6QopBJFKRUrwAAzmFrDK8sVEJVjyQbZ_jrvog1fehs7PXfR2KYBb0MfNeEsl4RyJf6DUsKokEoluvcPvQp9-uPmVuWUF5Su1JON6idzW-pF6-bQ3ujfDUvg2QZANNBUbWqHi3-czPM0ECy5F2t37Rp7s40TrFfzohdRr-ZFvz9fLUmP1jr1137damhrXQgmuP54dqzxWfFGnHwWqZhB9nTtKwgapm2q4PKcYpKnERMqPYX9AtMeyS4</recordid><startdate>201406</startdate><enddate>201406</enddate><creator>Wu, Meixiang</creator><creator>Gotoh, Hiroki</creator><creator>Waters, Timothy</creator><creator>Walsh, Douglas B</creator><creator>Lavine, Laura Corley</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QR</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><scope>RC3</scope></search><sort><creationdate>201406</creationdate><title>Identification of an alternative knockdown resistance (kdr)‐like mutation, M918L, and a novel mutation, V1010A, in the Thrips tabaci voltage‐gated sodium channel gene</title><author>Wu, Meixiang ; Gotoh, Hiroki ; Waters, Timothy ; Walsh, Douglas B ; Lavine, Laura Corley</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3538-1e07a980aa85ca29a547b72f5381965b1668976d8936aca3c02ec5fe59a194083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>alanine</topic><topic>Allium cepa</topic><topic>arthropod pests</topic><topic>Arthropoda</topic><topic>Arthropods</topic><topic>Bioassays</topic><topic>Biological and medical sciences</topic><topic>Control</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Knockdown Techniques</topic><topic>genes</topic><topic>Insecticide Resistance - genetics</topic><topic>Insecticides</topic><topic>kdr</topic><topic>lambda-cyhalothrin</topic><topic>leucine</topic><topic>methionine</topic><topic>Mutation</topic><topic>Nitriles - toxicity</topic><topic>Pest control</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>point mutation</topic><topic>Protozoa. Invertebrates</topic><topic>Pyrethrins - toxicity</topic><topic>pyrethroid resistance</topic><topic>sodium channel</topic><topic>sodium channels</topic><topic>Sodium Channels - genetics</topic><topic>Thrips tabaci</topic><topic>Thysanoptera - drug effects</topic><topic>Thysanoptera - genetics</topic><topic>valine</topic><topic>Washington</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Meixiang</creatorcontrib><creatorcontrib>Gotoh, Hiroki</creatorcontrib><creatorcontrib>Waters, Timothy</creatorcontrib><creatorcontrib>Walsh, Douglas B</creatorcontrib><creatorcontrib>Lavine, Laura Corley</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Genetics Abstracts</collection><jtitle>Pest management science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Meixiang</au><au>Gotoh, Hiroki</au><au>Waters, Timothy</au><au>Walsh, Douglas B</au><au>Lavine, Laura Corley</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of an alternative knockdown resistance (kdr)‐like mutation, M918L, and a novel mutation, V1010A, in the Thrips tabaci voltage‐gated sodium channel gene</atitle><jtitle>Pest management science</jtitle><addtitle>Pest. Manag. Sci</addtitle><date>2014-06</date><risdate>2014</risdate><volume>70</volume><issue>6</issue><spage>977</spage><epage>981</epage><pages>977-981</pages><issn>1526-498X</issn><eissn>1526-4998</eissn><coden>PMSCFC</coden><abstract>BACKGROUND: Knockdown resistance (kdr) has been identified as a main mechanism against pyrethroid insecticides in many arthropod pests including in the onion thrips, Thrips tabaci. To characterize and identify pyrethroid‐resistance in onion thrips in Washington state, we conducted insecticide bioassays and sequenced a region of the voltage gated sodium channel gene from several different T. tabaci populations. RESULTS: Field collected Thrips tabaci were found to have large variations in resistance to the pyrethroid insecticide lambda‐cyhalothrin. We identified two single nucleotide substitutions in our analysis of a partial sequence of the T. tabaci voltage‐gated sodium channel gene. One mutation resulted in the non‐synonymous substitution of methionine with leucine (M918L), which is well known to be responsible for super knockdown resistance in some pest species. Another non‐synonymous substitution, a valine (GTT) to alanine (GCT) replacement at amino acid 1010 (V1010A) was identified in our study and was associated with lambda‐cyhalothrin resistance. CONCLUSION: We have characterized a known kdr mutation and identified a novel mutation in the voltage‐gated sodium channel gene of Thrips tabaci associated with resistance to lambda‐cyhalothrin. This gene region and these mutations are expected to be useful in the development of a diagnostic test to detect kdr resistance in many onion thrips populations. © 2013 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>23983138</pmid><doi>10.1002/ps.3638</doi><tpages>5</tpages></addata></record> |
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| subjects | alanine Allium cepa arthropod pests Arthropoda Arthropods Bioassays Biological and medical sciences Control Fundamental and applied biological sciences. Psychology Gene Knockdown Techniques genes Insecticide Resistance - genetics Insecticides kdr lambda-cyhalothrin leucine methionine Mutation Nitriles - toxicity Pest control Phytopathology. Animal pests. Plant and forest protection point mutation Protozoa. Invertebrates Pyrethrins - toxicity pyrethroid resistance sodium channel sodium channels Sodium Channels - genetics Thrips tabaci Thysanoptera - drug effects Thysanoptera - genetics valine Washington |
| title | Identification of an alternative knockdown resistance (kdr)‐like mutation, M918L, and a novel mutation, V1010A, in the Thrips tabaci voltage‐gated sodium channel gene |
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