Purification and regulatory properties of the NADP-linked malic enzyme from Crithidia fasciculata

The NADP-linked malic enzyme (EC 1.1.1.40) from the insect flagellate Crithidia fasciculata has been purified to electrophoretic homogeneity by a procedure involving ammonium sulphate fractionation, gel filtration on Sephadex G-200, and column chromatography on DEAE-cellulose and hydroxylapatite. Th...

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Veröffentlicht in:	Molecular and biochemical parasitology 1981-01, Vol.3 (1), p.1-11
Hauptverfasser:	Orellano, Elena, Cazzulo, Juan José
Format:	Artikel
Sprache:	eng
Schlagworte:	activation Crithidia fasciculata Enzyme inhibitors and activators Enzyme purification and properties inhibition malate dehydrogenase (oxaloacetate-decarboxylating)(NADP super(+)) Malic enzyme purification Regulation of aerobic fermentation
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