Concordance of two serological tests for the diagnosis of Chagas disease
Establish the concordance between in-house ELISA and IIF for the diagnosis of infection with T. cruzi using blood eluates. A study of diagnostic technology evaluation and cross-sectional sample of 650 residents of an endemic area of Colombia was conducted. It was determined the Receiver Operating Ch...
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| Veröffentlicht in: | Revista de salud pública (Bogotá, Colombia) Colombia), 2013-03, Vol.15 (2), p.208-219 |
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| creator | Farfán-García, Ana E Castellanos-Domínguez, Yeny Z Luna-Marín, Katherine P Angulo-Silva, Víctor M |
| description | Establish the concordance between in-house ELISA and IIF for the diagnosis of infection with T. cruzi using blood eluates.
A study of diagnostic technology evaluation and cross-sectional sample of 650 residents of an endemic area of Colombia was conducted. It was determined the Receiver Operating Characteristic curve (ROC) and IIF was used as a gold standard. It was established the cutoff for the ELISA and the correlation between readings.
The in-house ELISA it was an agreement of 0.99 (95 % CI: 0.989 to 0.992) between the two readings taken and the area for the ROC curve was 0.9795. The cutoff was set at 0.5 absorbance in the ELISA test. 16.6 % were positive by ELISA and 10.9 % by IIF.
The in-house ELISA showed good concordance compared to the IIF, so it is a good choice diagnostic for the population living in remote areas. |
| format | Article |
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A study of diagnostic technology evaluation and cross-sectional sample of 650 residents of an endemic area of Colombia was conducted. It was determined the Receiver Operating Characteristic curve (ROC) and IIF was used as a gold standard. It was established the cutoff for the ELISA and the correlation between readings.
The in-house ELISA it was an agreement of 0.99 (95 % CI: 0.989 to 0.992) between the two readings taken and the area for the ROC curve was 0.9795. The cutoff was set at 0.5 absorbance in the ELISA test. 16.6 % were positive by ELISA and 10.9 % by IIF.
The in-house ELISA showed good concordance compared to the IIF, so it is a good choice diagnostic for the population living in remote areas.</description><identifier>ISSN: 0124-0064</identifier><identifier>PMID: 24892663</identifier><language>spa</language><publisher>Colombia</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Chagas Disease - blood ; Chagas Disease - diagnosis ; Child ; Child, Preschool ; Cross-Sectional Studies ; Enzyme-Linked Immunosorbent Assay ; Fluorescent Antibody Technique, Indirect ; Humans ; Infant ; Middle Aged ; Serologic Tests ; Young Adult</subject><ispartof>Revista de salud pública (Bogotá, Colombia), 2013-03, Vol.15 (2), p.208-219</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24892663$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Farfán-García, Ana E</creatorcontrib><creatorcontrib>Castellanos-Domínguez, Yeny Z</creatorcontrib><creatorcontrib>Luna-Marín, Katherine P</creatorcontrib><creatorcontrib>Angulo-Silva, Víctor M</creatorcontrib><title>Concordance of two serological tests for the diagnosis of Chagas disease</title><title>Revista de salud pública (Bogotá, Colombia)</title><addtitle>Rev Salud Publica (Bogota)</addtitle><description>Establish the concordance between in-house ELISA and IIF for the diagnosis of infection with T. cruzi using blood eluates.
A study of diagnostic technology evaluation and cross-sectional sample of 650 residents of an endemic area of Colombia was conducted. It was determined the Receiver Operating Characteristic curve (ROC) and IIF was used as a gold standard. It was established the cutoff for the ELISA and the correlation between readings.
The in-house ELISA it was an agreement of 0.99 (95 % CI: 0.989 to 0.992) between the two readings taken and the area for the ROC curve was 0.9795. The cutoff was set at 0.5 absorbance in the ELISA test. 16.6 % were positive by ELISA and 10.9 % by IIF.
The in-house ELISA showed good concordance compared to the IIF, so it is a good choice diagnostic for the population living in remote areas.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Chagas Disease - blood</subject><subject>Chagas Disease - diagnosis</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cross-Sectional Studies</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Humans</subject><subject>Infant</subject><subject>Middle Aged</subject><subject>Serologic Tests</subject><subject>Young Adult</subject><issn>0124-0064</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1j01Lw0AURWeh2Fr9CzJLN4H5TmYpQa1QcNN9eEnepJEkE-eliP_eiHV14HK43HvFtkIqkwnhzIbdEn0Ioby0_oZtlCm8ck5v2b6MUxNTC1ODPAa-fEVOmOIQu76BgS9IC_EQE19OyNseuilST79qeYIOaM0IgfCOXQcYCO8v3LHjy_Ox3GeH99e38umQzdbpDE3usJVgvbAiGK2NL4SWweqikCp3EMA7WxcrdLA5CON0kNjmMhjMa6N37PGvdk7x87yOq8aeGhwGmDCeqZJWK2-csWJVHy7quR6xrebUj5C-q__z-gdtwlOo</recordid><startdate>201303</startdate><enddate>201303</enddate><creator>Farfán-García, Ana E</creator><creator>Castellanos-Domínguez, Yeny Z</creator><creator>Luna-Marín, Katherine P</creator><creator>Angulo-Silva, Víctor M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201303</creationdate><title>Concordance of two serological tests for the diagnosis of Chagas disease</title><author>Farfán-García, Ana E ; Castellanos-Domínguez, Yeny Z ; Luna-Marín, Katherine P ; Angulo-Silva, Víctor M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p563-e476ed1a59050f433498031f53881276afa965b8fa93f57a0463f1ed71f4e7b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>spa</language><creationdate>2013</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Chagas Disease - blood</topic><topic>Chagas Disease - diagnosis</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cross-Sectional Studies</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Humans</topic><topic>Infant</topic><topic>Middle Aged</topic><topic>Serologic Tests</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Farfán-García, Ana E</creatorcontrib><creatorcontrib>Castellanos-Domínguez, Yeny Z</creatorcontrib><creatorcontrib>Luna-Marín, Katherine P</creatorcontrib><creatorcontrib>Angulo-Silva, Víctor M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Revista de salud pública (Bogotá, Colombia)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Farfán-García, Ana E</au><au>Castellanos-Domínguez, Yeny Z</au><au>Luna-Marín, Katherine P</au><au>Angulo-Silva, Víctor M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Concordance of two serological tests for the diagnosis of Chagas disease</atitle><jtitle>Revista de salud pública (Bogotá, Colombia)</jtitle><addtitle>Rev Salud Publica (Bogota)</addtitle><date>2013-03</date><risdate>2013</risdate><volume>15</volume><issue>2</issue><spage>208</spage><epage>219</epage><pages>208-219</pages><issn>0124-0064</issn><abstract>Establish the concordance between in-house ELISA and IIF for the diagnosis of infection with T. cruzi using blood eluates.
A study of diagnostic technology evaluation and cross-sectional sample of 650 residents of an endemic area of Colombia was conducted. It was determined the Receiver Operating Characteristic curve (ROC) and IIF was used as a gold standard. It was established the cutoff for the ELISA and the correlation between readings.
The in-house ELISA it was an agreement of 0.99 (95 % CI: 0.989 to 0.992) between the two readings taken and the area for the ROC curve was 0.9795. The cutoff was set at 0.5 absorbance in the ELISA test. 16.6 % were positive by ELISA and 10.9 % by IIF.
The in-house ELISA showed good concordance compared to the IIF, so it is a good choice diagnostic for the population living in remote areas.</abstract><cop>Colombia</cop><pmid>24892663</pmid><tpages>12</tpages></addata></record> |
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| ispartof | Revista de salud pública (Bogotá, Colombia), 2013-03, Vol.15 (2), p.208-219 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Adolescent Adult Aged Aged, 80 and over Chagas Disease - blood Chagas Disease - diagnosis Child Child, Preschool Cross-Sectional Studies Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique, Indirect Humans Infant Middle Aged Serologic Tests Young Adult |
| title | Concordance of two serological tests for the diagnosis of Chagas disease |
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