Uninfected Cell Polymerase Efficiently Transcribes Early but not Late Herpes Simplex Virus Type 1 mRNA
The sequences of the DNAs encoding the 5′ends of one early and one late herpes simplex virus type 1 mRNA were analyzed, and the 5′ends of these mRNA species were precisely located. Neither mRNA species is spliced and the noncoding strand of the DNA contains recognizable T-A-T-A and C-A-T boxes upstr...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1981-10, Vol.78 (10), p.6139-6143 |
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| container_issue | 10 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 78 |
| creator | Frink, Raymond J. Draper, Kenneth G. Wagner, Edward K. |
| description | The sequences of the DNAs encoding the 5′ends of one early and one late herpes simplex virus type 1 mRNA were analyzed, and the 5′ends of these mRNA species were precisely located. Neither mRNA species is spliced and the noncoding strand of the DNA contains recognizable T-A-T-A and C-A-T boxes upstream from their respective 5′ends. The early mRNA was efficiently transcribed by a commercially available uninfected cell lysate system, but the late mRNA was not. This difference between early and late mRNAs appears to be general in this virus. |
| doi_str_mv | 10.1073/pnas.78.10.6139 |
| format | Article |
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Neither mRNA species is spliced and the noncoding strand of the DNA contains recognizable T-A-T-A and C-A-T boxes upstream from their respective 5′ends. The early mRNA was efficiently transcribed by a commercially available uninfected cell lysate system, but the late mRNA was not. This difference between early and late mRNAs appears to be general in this virus.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.78.10.6139</identifier><identifier>PMID: 6273852</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Base Sequence ; Cell Transformation, Viral ; DNA ; DNA Restriction Enzymes ; DNA, Viral - genetics ; DNA-Directed RNA Polymerases - metabolism ; Gels ; Gene expression ; Genes ; Genes, Viral ; HeLa cells ; HeLa Cells - metabolism ; herpes simplex virus 1 ; Human herpesvirus 1 ; Humans ; Hybridity ; Kinetics ; Messenger RNA ; Nucleotide sequences ; RNA ; RNA, Messenger - genetics ; Simplexvirus - genetics ; Transcription, Genetic</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1981-10, Vol.78 (10), p.6139-6143</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4079-8ae7faba96046ee0364ca41560bff9d0c4f234a2c5e325190240b5a4291f79a03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/78/10.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/11022$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/11022$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6273852$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Frink, Raymond J.</creatorcontrib><creatorcontrib>Draper, Kenneth G.</creatorcontrib><creatorcontrib>Wagner, Edward K.</creatorcontrib><title>Uninfected Cell Polymerase Efficiently Transcribes Early but not Late Herpes Simplex Virus Type 1 mRNA</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The sequences of the DNAs encoding the 5′ends of one early and one late herpes simplex virus type 1 mRNA were analyzed, and the 5′ends of these mRNA species were precisely located. 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This difference between early and late mRNAs appears to be general in this virus.</description><subject>Base Sequence</subject><subject>Cell Transformation, Viral</subject><subject>DNA</subject><subject>DNA Restriction Enzymes</subject><subject>DNA, Viral - genetics</subject><subject>DNA-Directed RNA Polymerases - metabolism</subject><subject>Gels</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Genes, Viral</subject><subject>HeLa cells</subject><subject>HeLa Cells - metabolism</subject><subject>herpes simplex virus 1</subject><subject>Human herpesvirus 1</subject><subject>Humans</subject><subject>Hybridity</subject><subject>Kinetics</subject><subject>Messenger RNA</subject><subject>Nucleotide sequences</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>Simplexvirus - genetics</subject><subject>Transcription, Genetic</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EKkNhjYQE8gpWmV4_EscLFtVoSpFGgGDK1nI815Aqr9oJ6vz7OpqhhQ2srOvznatjH0JeMlgyUOJs6GxcqjINy4IJ_YgsGGiWFVLDY7IA4CorJZdPybMYrwFA5yWckJOCK1HmfEH8VVd3Ht2IO7rCpqFf-mbfYrAR6dr72tXYjc2eboPtogt1hZGubUg31TTSrh_pxo5ILzEMSflWt0ODt_R7HaZIt_sBKaPt10_nz8kTb5uIL47nKbm6WG9Xl9nm84ePq_NN5iQonZUWlbeV1QXIAhFEIZ2VLC-g8l7vwEnPhbTc5Sh4zjRwCVVuJdfMK21BnJL3h73DVLW4cyl8sI0ZQt3asDe9rc3fSlf_ND_6X0bIUmuR_G-P_tDfTBhH09bRpX-xHfZTNEqokin-f5DlghdCzInODqALfYwB_X0YBmau0MwVGlXO81xhcrz-8w33_LGzpL876rPxt_qwwPipaUa8HRP55p9kAl4dgOs49uEhGQPOxR15fbk8</recordid><startdate>19811001</startdate><enddate>19811001</enddate><creator>Frink, Raymond J.</creator><creator>Draper, Kenneth G.</creator><creator>Wagner, Edward K.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19811001</creationdate><title>Uninfected Cell Polymerase Efficiently Transcribes Early but not Late Herpes Simplex Virus Type 1 mRNA</title><author>Frink, Raymond J. ; 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Neither mRNA species is spliced and the noncoding strand of the DNA contains recognizable T-A-T-A and C-A-T boxes upstream from their respective 5′ends. The early mRNA was efficiently transcribed by a commercially available uninfected cell lysate system, but the late mRNA was not. This difference between early and late mRNAs appears to be general in this virus.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6273852</pmid><doi>10.1073/pnas.78.10.6139</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1981-10, Vol.78 (10), p.6139-6143 |
| issn | 0027-8424 1091-6490 |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Base Sequence Cell Transformation, Viral DNA DNA Restriction Enzymes DNA, Viral - genetics DNA-Directed RNA Polymerases - metabolism Gels Gene expression Genes Genes, Viral HeLa cells HeLa Cells - metabolism herpes simplex virus 1 Human herpesvirus 1 Humans Hybridity Kinetics Messenger RNA Nucleotide sequences RNA RNA, Messenger - genetics Simplexvirus - genetics Transcription, Genetic |
| title | Uninfected Cell Polymerase Efficiently Transcribes Early but not Late Herpes Simplex Virus Type 1 mRNA |
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