Effect of additives on the thermostability of Bacillus stearothermophilus α-amylase
The effect of additives on the thermostability of Bacillus stearothermophilus alpha -amylase was determined. Polyols, dimethyl formamide, and dimethyl sulfoxide all increased the half life of the enzyme approximately 2-fold when tested at a 10% (w/v) addition. These results suggest that the enzyme&#...
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| Veröffentlicht in: | Biotechnology letters 1989-08, Vol.11 (8), p.541-544 |
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| container_title | Biotechnology letters |
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| creator | BRUMM, P. J TEAGUE, W. M |
| description | The effect of additives on the thermostability of Bacillus stearothermophilus alpha -amylase was determined. Polyols, dimethyl formamide, and dimethyl sulfoxide all increased the half life of the enzyme approximately 2-fold when tested at a 10% (w/v) addition. These results suggest that the enzyme's structure is stabilized against thermal denaturation through ionic interactions. Addition of dextran or polyvinyl alcohol (hydrophilic polymers which increase the viscosity of the solution) had a slight positive effect on enzyme stability while addition of polyethylene glycol or polyvinylpyrrolidone (hydrophobic polymers which increase the viscosity of the solution) resulted in a 2-fold decrease in enzyme half life. |
| doi_str_mv | 10.1007/BF01040032 |
| format | Article |
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Addition of dextran or polyvinyl alcohol (hydrophilic polymers which increase the viscosity of the solution) had a slight positive effect on enzyme stability while addition of polyethylene glycol or polyvinylpyrrolidone (hydrophobic polymers which increase the viscosity of the solution) resulted in a 2-fold decrease in enzyme half life.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/BF01040032</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Bacillus stearothermophilus ; Bacteriology ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; dextran ; dimethyl formamide ; dimethyl sulfoxide ; Fundamental and applied biological sciences. Psychology ; Metabolism. 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Addition of dextran or polyvinyl alcohol (hydrophilic polymers which increase the viscosity of the solution) had a slight positive effect on enzyme stability while addition of polyethylene glycol or polyvinylpyrrolidone (hydrophobic polymers which increase the viscosity of the solution) resulted in a 2-fold decrease in enzyme half life.</description><subject>Bacillus stearothermophilus</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>dextran</subject><subject>dimethyl formamide</subject><subject>dimethyl sulfoxide</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Metabolism. 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Enzymes</topic><topic>Microbiology</topic><topic>Mission oriented research</topic><topic>Physiology and metabolism</topic><topic>polyethylene glycol</topic><topic>polyols</topic><topic>polyvinyl alcohol</topic><topic>povidone</topic><topic>thermal stability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BRUMM, P. J</creatorcontrib><creatorcontrib>TEAGUE, W. 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Addition of dextran or polyvinyl alcohol (hydrophilic polymers which increase the viscosity of the solution) had a slight positive effect on enzyme stability while addition of polyethylene glycol or polyvinylpyrrolidone (hydrophobic polymers which increase the viscosity of the solution) resulted in a 2-fold decrease in enzyme half life.</abstract><cop>Dordrecht</cop><pub>Springer</pub><doi>10.1007/BF01040032</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 0141-5492 |
| ispartof | Biotechnology letters, 1989-08, Vol.11 (8), p.541-544 |
| issn | 0141-5492 1573-6776 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15322276 |
| source | SpringerLink Journals |
| subjects | Bacillus stearothermophilus Bacteriology Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology dextran dimethyl formamide dimethyl sulfoxide Fundamental and applied biological sciences. Psychology Metabolism. Enzymes Microbiology Mission oriented research Physiology and metabolism polyethylene glycol polyols polyvinyl alcohol povidone thermal stability |
| title | Effect of additives on the thermostability of Bacillus stearothermophilus α-amylase |
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