Collagen Gel Contraction Assay Using Human Bronchial Smooth Muscle Cells and Its Application for Evaluation of Inhibitory Effect of Formoterol

Collagen gel contraction assay is a method for evaluating contraction of cells embedded in collagen gel matrices through measuring the gel size. In the present study, we established a protocol for collagen gel contraction assay using human bronchial smooth muscle cells obtained commercially, and app...

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Veröffentlicht in:	Biological & pharmaceutical bulletin 2014/06/01, Vol.37(6), pp.1014-1020
Hauptverfasser:	Sakota, Yusuke, Ozawa, Yuji, Yamashita, Hirotaka, Tanaka, Hiroyuki, Inagaki, Naoki
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological Assay - methods Bronchi - drug effects Bronchodilator Agents - pharmacology Cells, Cultured Collagen - chemistry contraction assay Ethanolamines - pharmacology fluticasone formoterol Formoterol Fumarate Gels histamine human bronchial smooth muscle cell Humans Muscle Contraction - drug effects Muscle, Smooth - drug effects Myocytes, Smooth Muscle - drug effects
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