Structural Alterations of the DNA in Cerebellar Neurons after Whole-Brain Irradiation
Male Sprague-Dawley rats weighing 260-280 g were whole-brain-irradiated with X-ray doses of 433, 867, 1083, 1300, 1516, and 1713 rad. Over the next 2.25 years rats were killed at various times, and the state of the DNA in their cerebellar neurons was examined by sedimentation through alkaline sucros...
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| Veröffentlicht in: | Radiat. Res.; (United States) 1981-03, Vol.85 (3), p.465-471 |
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| creator | Wheeler, Kenneth T. Weinstein, Ralph E. Kaufman, Kenneth Ritter, Paul |
| description | Male Sprague-Dawley rats weighing 260-280 g were whole-brain-irradiated with X-ray doses of 433, 867, 1083, 1300, 1516, and 1713 rad. Over the next 2.25 years rats were killed at various times, and the state of the DNA in their cerebellar neurons was examined by sedimentation through alkaline sucrose gradients in reorienting zonal rotors. The data were analyzed as the percentage of the sedimenting DNA with sedimentation coefficients greater than 300 S, an arbitrarily selected category of no defined molecular significance. The general pattern at all doses consisted first of a slow return to the unirradiated DNA state (≥4 weeks) that was relatively dose dependent. This was followed by an increase in the amount of DNA sedimenting >300 S; both the extent and time course of this increase appeared to be dose dependent. Finally, the DNA degraded at a relatively dose independent rate. There was little change in the neuronal DNA from unirradiated rats during this study. The data suggest that increases in the amount of fast-sedimenting DNA observed 30-80 weeks after low to moderate doses of whole-brain irradiation represent a type of DNA damage rather than repair and that this damage ultimately results in degradation of the neuronal DNA and death of the rat. |
| doi_str_mv | 10.2307/3575417 |
| format | Article |
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Over the next 2.25 years rats were killed at various times, and the state of the DNA in their cerebellar neurons was examined by sedimentation through alkaline sucrose gradients in reorienting zonal rotors. The data were analyzed as the percentage of the sedimenting DNA with sedimentation coefficients greater than 300 S, an arbitrarily selected category of no defined molecular significance. The general pattern at all doses consisted first of a slow return to the unirradiated DNA state (≥4 weeks) that was relatively dose dependent. This was followed by an increase in the amount of DNA sedimenting >300 S; both the extent and time course of this increase appeared to be dose dependent. Finally, the DNA degraded at a relatively dose independent rate. There was little change in the neuronal DNA from unirradiated rats during this study. The data suggest that increases in the amount of fast-sedimenting DNA observed 30-80 weeks after low to moderate doses of whole-brain irradiation represent a type of DNA damage rather than repair and that this damage ultimately results in degradation of the neuronal DNA and death of the rat.</description><identifier>ISSN: 0033-7587</identifier><identifier>EISSN: 1938-5404</identifier><identifier>DOI: 10.2307/3575417</identifier><identifier>PMID: 7208807</identifier><language>eng</language><publisher>United States: Academic Press, Inc</publisher><subject>560114 - Radiation Effects on Biochemicals- In Animals- (-1987) ; 560152 - Radiation Effects on Animals- Animals ; Aging ; ANIMAL CELLS ; ANIMALS ; BIOLOGICAL EFFECTS ; BIOLOGICAL RADIATION EFFECTS ; BODY ; BRAIN ; Censorship ; CENTRAL NERVOUS SYSTEM ; Centrifugation, Density Gradient ; CEREBELLUM ; Cerebellum - radiation effects ; Cerebellum - ultrastructure ; CHEMICAL RADIATION EFFECTS ; CHEMICAL REACTIONS ; CHEMISTRY ; DATA ANALYSIS ; DECOMPOSITION ; DNA ; DNA - analysis ; DNA - radiation effects ; DNA damage ; DNA Repair ; DOSE-RESPONSE RELATIONSHIPS ; DOSES ; ELECTROMAGNETIC RADIATION ; EXTERNAL IRRADIATION ; IONIZING RADIATIONS ; IRRADIATION ; Kaplan Meier estimator ; Male ; MAMMALS ; NERVE CELLS ; NERVOUS SYSTEM ; Neurons ; Neurons - analysis ; Neurons - radiation effects ; Neurons - ultrastructure ; NUCLEIC ACIDS ; ORGANIC COMPOUNDS ; ORGANS ; PARTIAL BODY IRRADIATION ; RADIATION CHEMISTRY ; Radiation damage ; Radiation Dosage ; RADIATION DOSES ; RADIATION EFFECTS ; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT ; RADIATIONS ; RADIOLYSIS ; Radiotherapy ; RATS ; RODENTS ; SEDIMENTATION ; SOMATIC CELLS ; Statistical median ; Tumors ; VERTEBRATES ; X RADIATION ; X-Rays</subject><ispartof>Radiat. Res.; (United States), 1981-03, Vol.85 (3), p.465-471</ispartof><rights>Copyright 1981 Academic Press, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-ae0473d4bbccc6f8001a6d0ebd27f342f2462ac2be4416413a9cf283c4b0654f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3575417$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3575417$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7208807$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6537929$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Wheeler, Kenneth T.</creatorcontrib><creatorcontrib>Weinstein, Ralph E.</creatorcontrib><creatorcontrib>Kaufman, Kenneth</creatorcontrib><creatorcontrib>Ritter, Paul</creatorcontrib><creatorcontrib>Univ. of Rochester Cancer Center, NY</creatorcontrib><title>Structural Alterations of the DNA in Cerebellar Neurons after Whole-Brain Irradiation</title><title>Radiat. Res.; (United States)</title><addtitle>Radiat Res</addtitle><description>Male Sprague-Dawley rats weighing 260-280 g were whole-brain-irradiated with X-ray doses of 433, 867, 1083, 1300, 1516, and 1713 rad. Over the next 2.25 years rats were killed at various times, and the state of the DNA in their cerebellar neurons was examined by sedimentation through alkaline sucrose gradients in reorienting zonal rotors. The data were analyzed as the percentage of the sedimenting DNA with sedimentation coefficients greater than 300 S, an arbitrarily selected category of no defined molecular significance. The general pattern at all doses consisted first of a slow return to the unirradiated DNA state (≥4 weeks) that was relatively dose dependent. This was followed by an increase in the amount of DNA sedimenting >300 S; both the extent and time course of this increase appeared to be dose dependent. Finally, the DNA degraded at a relatively dose independent rate. There was little change in the neuronal DNA from unirradiated rats during this study. The data suggest that increases in the amount of fast-sedimenting DNA observed 30-80 weeks after low to moderate doses of whole-brain irradiation represent a type of DNA damage rather than repair and that this damage ultimately results in degradation of the neuronal DNA and death of the rat.</description><subject>560114 - Radiation Effects on Biochemicals- In Animals- (-1987)</subject><subject>560152 - Radiation Effects on Animals- Animals</subject><subject>Aging</subject><subject>ANIMAL CELLS</subject><subject>ANIMALS</subject><subject>BIOLOGICAL EFFECTS</subject><subject>BIOLOGICAL RADIATION EFFECTS</subject><subject>BODY</subject><subject>BRAIN</subject><subject>Censorship</subject><subject>CENTRAL NERVOUS SYSTEM</subject><subject>Centrifugation, Density Gradient</subject><subject>CEREBELLUM</subject><subject>Cerebellum - radiation effects</subject><subject>Cerebellum - ultrastructure</subject><subject>CHEMICAL RADIATION EFFECTS</subject><subject>CHEMICAL REACTIONS</subject><subject>CHEMISTRY</subject><subject>DATA ANALYSIS</subject><subject>DECOMPOSITION</subject><subject>DNA</subject><subject>DNA - analysis</subject><subject>DNA - radiation effects</subject><subject>DNA damage</subject><subject>DNA Repair</subject><subject>DOSE-RESPONSE RELATIONSHIPS</subject><subject>DOSES</subject><subject>ELECTROMAGNETIC RADIATION</subject><subject>EXTERNAL IRRADIATION</subject><subject>IONIZING RADIATIONS</subject><subject>IRRADIATION</subject><subject>Kaplan Meier estimator</subject><subject>Male</subject><subject>MAMMALS</subject><subject>NERVE CELLS</subject><subject>NERVOUS SYSTEM</subject><subject>Neurons</subject><subject>Neurons - analysis</subject><subject>Neurons - radiation effects</subject><subject>Neurons - ultrastructure</subject><subject>NUCLEIC ACIDS</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANS</subject><subject>PARTIAL BODY IRRADIATION</subject><subject>RADIATION CHEMISTRY</subject><subject>Radiation damage</subject><subject>Radiation Dosage</subject><subject>RADIATION DOSES</subject><subject>RADIATION EFFECTS</subject><subject>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</subject><subject>RADIATIONS</subject><subject>RADIOLYSIS</subject><subject>Radiotherapy</subject><subject>RATS</subject><subject>RODENTS</subject><subject>SEDIMENTATION</subject><subject>SOMATIC CELLS</subject><subject>Statistical median</subject><subject>Tumors</subject><subject>VERTEBRATES</subject><subject>X RADIATION</subject><subject>X-Rays</subject><issn>0033-7587</issn><issn>1938-5404</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10F1LwzAUBuAgypwf-AuEIqJX1Xw1aS_n_BqMeaHDy5CmJ6yja2aSXvjv7ezwzqtDOE9eOC9CFwTfUYblPctkxok8QGNSsDzNOOaHaIwxY6nMcnmMTkJY4_5NRDFCI0lxnmM5Rsv36DsTO6-bZNJE8DrWrg2Js0lcQfK4mCR1m0zBQwlNo32ygM7vgLY9Tj5XroH0wesezbzXVf37_wwdWd0EON_PU7R8fvqYvqbzt5fZdDJPDRMsphowl6ziZWmMETbHmGhRYSgrKi3j1FIuqDa0BM6J4ITpwliaM8NLLDJu2Sm6GnJdiLUKpo5gVsa1LZioRMZkQYse3Qxo691XByGqTR3M7poWXBcUyRjJSYF7eDtA410IHqza-nqj_bciWO1aVvuWe3m5j-zKDVR_bl9rv78e9usQnf835geDDoD2</recordid><startdate>198103</startdate><enddate>198103</enddate><creator>Wheeler, Kenneth T.</creator><creator>Weinstein, Ralph E.</creator><creator>Kaufman, Kenneth</creator><creator>Ritter, Paul</creator><general>Academic Press, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>OTOTI</scope></search><sort><creationdate>198103</creationdate><title>Structural Alterations of the DNA in Cerebellar Neurons after Whole-Brain Irradiation</title><author>Wheeler, Kenneth T. ; Weinstein, Ralph E. ; Kaufman, Kenneth ; Ritter, Paul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-ae0473d4bbccc6f8001a6d0ebd27f342f2462ac2be4416413a9cf283c4b0654f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>560114 - Radiation Effects on Biochemicals- In Animals- (-1987)</topic><topic>560152 - Radiation Effects on Animals- Animals</topic><topic>Aging</topic><topic>ANIMAL CELLS</topic><topic>ANIMALS</topic><topic>BIOLOGICAL EFFECTS</topic><topic>BIOLOGICAL RADIATION EFFECTS</topic><topic>BODY</topic><topic>BRAIN</topic><topic>Censorship</topic><topic>CENTRAL NERVOUS SYSTEM</topic><topic>Centrifugation, Density Gradient</topic><topic>CEREBELLUM</topic><topic>Cerebellum - radiation effects</topic><topic>Cerebellum - ultrastructure</topic><topic>CHEMICAL RADIATION EFFECTS</topic><topic>CHEMICAL REACTIONS</topic><topic>CHEMISTRY</topic><topic>DATA ANALYSIS</topic><topic>DECOMPOSITION</topic><topic>DNA</topic><topic>DNA - analysis</topic><topic>DNA - radiation effects</topic><topic>DNA damage</topic><topic>DNA Repair</topic><topic>DOSE-RESPONSE RELATIONSHIPS</topic><topic>DOSES</topic><topic>ELECTROMAGNETIC RADIATION</topic><topic>EXTERNAL IRRADIATION</topic><topic>IONIZING RADIATIONS</topic><topic>IRRADIATION</topic><topic>Kaplan Meier estimator</topic><topic>Male</topic><topic>MAMMALS</topic><topic>NERVE CELLS</topic><topic>NERVOUS SYSTEM</topic><topic>Neurons</topic><topic>Neurons - analysis</topic><topic>Neurons - radiation effects</topic><topic>Neurons - ultrastructure</topic><topic>NUCLEIC ACIDS</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANS</topic><topic>PARTIAL BODY IRRADIATION</topic><topic>RADIATION CHEMISTRY</topic><topic>Radiation damage</topic><topic>Radiation Dosage</topic><topic>RADIATION DOSES</topic><topic>RADIATION EFFECTS</topic><topic>RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT</topic><topic>RADIATIONS</topic><topic>RADIOLYSIS</topic><topic>Radiotherapy</topic><topic>RATS</topic><topic>RODENTS</topic><topic>SEDIMENTATION</topic><topic>SOMATIC CELLS</topic><topic>Statistical median</topic><topic>Tumors</topic><topic>VERTEBRATES</topic><topic>X RADIATION</topic><topic>X-Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wheeler, Kenneth T.</creatorcontrib><creatorcontrib>Weinstein, Ralph E.</creatorcontrib><creatorcontrib>Kaufman, Kenneth</creatorcontrib><creatorcontrib>Ritter, Paul</creatorcontrib><creatorcontrib>Univ. of Rochester Cancer Center, NY</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>OSTI.GOV</collection><jtitle>Radiat. Res.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wheeler, Kenneth T.</au><au>Weinstein, Ralph E.</au><au>Kaufman, Kenneth</au><au>Ritter, Paul</au><aucorp>Univ. of Rochester Cancer Center, NY</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural Alterations of the DNA in Cerebellar Neurons after Whole-Brain Irradiation</atitle><jtitle>Radiat. Res.; (United States)</jtitle><addtitle>Radiat Res</addtitle><date>1981-03</date><risdate>1981</risdate><volume>85</volume><issue>3</issue><spage>465</spage><epage>471</epage><pages>465-471</pages><issn>0033-7587</issn><eissn>1938-5404</eissn><abstract>Male Sprague-Dawley rats weighing 260-280 g were whole-brain-irradiated with X-ray doses of 433, 867, 1083, 1300, 1516, and 1713 rad. Over the next 2.25 years rats were killed at various times, and the state of the DNA in their cerebellar neurons was examined by sedimentation through alkaline sucrose gradients in reorienting zonal rotors. The data were analyzed as the percentage of the sedimenting DNA with sedimentation coefficients greater than 300 S, an arbitrarily selected category of no defined molecular significance. The general pattern at all doses consisted first of a slow return to the unirradiated DNA state (≥4 weeks) that was relatively dose dependent. This was followed by an increase in the amount of DNA sedimenting >300 S; both the extent and time course of this increase appeared to be dose dependent. Finally, the DNA degraded at a relatively dose independent rate. There was little change in the neuronal DNA from unirradiated rats during this study. The data suggest that increases in the amount of fast-sedimenting DNA observed 30-80 weeks after low to moderate doses of whole-brain irradiation represent a type of DNA damage rather than repair and that this damage ultimately results in degradation of the neuronal DNA and death of the rat.</abstract><cop>United States</cop><pub>Academic Press, Inc</pub><pmid>7208807</pmid><doi>10.2307/3575417</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0033-7587 |
| ispartof | Radiat. Res.; (United States), 1981-03, Vol.85 (3), p.465-471 |
| issn | 0033-7587 1938-5404 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15318190 |
| source | Jstor Complete Legacy; MEDLINE |
| subjects | 560114 - Radiation Effects on Biochemicals- In Animals- (-1987) 560152 - Radiation Effects on Animals- Animals Aging ANIMAL CELLS ANIMALS BIOLOGICAL EFFECTS BIOLOGICAL RADIATION EFFECTS BODY BRAIN Censorship CENTRAL NERVOUS SYSTEM Centrifugation, Density Gradient CEREBELLUM Cerebellum - radiation effects Cerebellum - ultrastructure CHEMICAL RADIATION EFFECTS CHEMICAL REACTIONS CHEMISTRY DATA ANALYSIS DECOMPOSITION DNA DNA - analysis DNA - radiation effects DNA damage DNA Repair DOSE-RESPONSE RELATIONSHIPS DOSES ELECTROMAGNETIC RADIATION EXTERNAL IRRADIATION IONIZING RADIATIONS IRRADIATION Kaplan Meier estimator Male MAMMALS NERVE CELLS NERVOUS SYSTEM Neurons Neurons - analysis Neurons - radiation effects Neurons - ultrastructure NUCLEIC ACIDS ORGANIC COMPOUNDS ORGANS PARTIAL BODY IRRADIATION RADIATION CHEMISTRY Radiation damage Radiation Dosage RADIATION DOSES RADIATION EFFECTS RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT RADIATIONS RADIOLYSIS Radiotherapy RATS RODENTS SEDIMENTATION SOMATIC CELLS Statistical median Tumors VERTEBRATES X RADIATION X-Rays |
| title | Structural Alterations of the DNA in Cerebellar Neurons after Whole-Brain Irradiation |
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