Genipin crosslinking elevates the strength of electrochemically aligned collagen to the level of tendons
Collagen-based tissue mimics are important in clinical research because collagen is the main structural element in tendons. The current study aimed to improve the mechanical strength of Electronically Aligned Collagen (ELAC) threads by optimizing several crosslinking parameters. The results indicate...
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| Veröffentlicht in: | Journal of the mechanical behavior of biomedical materials 2012-11, Vol.15, p.176-189 |
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| creator | Alfredo Uquillas, Jorge Kishore, Vipuil Akkus, Ozan |
| description | Collagen-based tissue mimics are important in clinical research because collagen is the main structural element in tendons. The current study aimed to improve the mechanical strength of Electronically Aligned Collagen (ELAC) threads by optimizing several crosslinking parameters. The results indicated that elevating the concentration of genipin to 2% and the solvent to 90% ethanol significantly enhanced the wet ultimate tensile stress of ELAC threads to 109MPa with a crosslinking degree of 65%. Furthermore, significantly higher adhesion and proliferation of hMSCs was observed in ELAC threads crosslinked with 2% genipin in 90% ethanol compared to 0.625% genipin in 1X PBS. In conclusion, ELAC threads with mechanical strength on par with native tendon have significant potential to be used as scaffolds in tendon tissue engineering applications.
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► The mechanical properties of ELAC threads were similar to those of native tendon. ► Significant proliferation/adhesion of human stem cells on ELAC threads were evidenced. ► PBS solvents decreased the mechanical properties of ELAC threads. ► PBS solvents decreased the proliferation rate of human stem cells on ELAC threads. |
| doi_str_mv | 10.1016/j.jmbbm.2012.06.012 |
| format | Article |
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[Display omitted]
► The mechanical properties of ELAC threads were similar to those of native tendon. ► Significant proliferation/adhesion of human stem cells on ELAC threads were evidenced. ► PBS solvents decreased the mechanical properties of ELAC threads. ► PBS solvents decreased the proliferation rate of human stem cells on ELAC threads.</description><identifier>ISSN: 1751-6161</identifier><identifier>EISSN: 1878-0180</identifier><identifier>DOI: 10.1016/j.jmbbm.2012.06.012</identifier><identifier>PMID: 23032437</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Alignment ; Biomechanical Phenomena ; Biomimetic Materials - chemistry ; Biomimetic Materials - pharmacology ; Cell Adhesion - drug effects ; Cell Proliferation - drug effects ; Collagen - chemistry ; Collagen - pharmacology ; Collagens ; Crosslinking ; Electrochemically aligned collagen ; Electrochemistry ; Ethanol ; Ethanol - chemistry ; Ethyl alcohol ; Genipin ; Human mesenchymal stem cell mechanical properties ; Humans ; Iridoids - chemistry ; Mechanical Phenomena ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - drug effects ; Strength ; Tendons ; Time Factors ; Tissue engineering</subject><ispartof>Journal of the mechanical behavior of biomedical materials, 2012-11, Vol.15, p.176-189</ispartof><rights>2012 Elsevier Ltd</rights><rights>Copyright © 2012 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-ea48f80c09d21ddd8bdb12f1625342a3819328a5dfa7800168d2fd3e7535efb83</citedby><cites>FETCH-LOGICAL-c458t-ea48f80c09d21ddd8bdb12f1625342a3819328a5dfa7800168d2fd3e7535efb83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jmbbm.2012.06.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23032437$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alfredo Uquillas, Jorge</creatorcontrib><creatorcontrib>Kishore, Vipuil</creatorcontrib><creatorcontrib>Akkus, Ozan</creatorcontrib><title>Genipin crosslinking elevates the strength of electrochemically aligned collagen to the level of tendons</title><title>Journal of the mechanical behavior of biomedical materials</title><addtitle>J Mech Behav Biomed Mater</addtitle><description>Collagen-based tissue mimics are important in clinical research because collagen is the main structural element in tendons. The current study aimed to improve the mechanical strength of Electronically Aligned Collagen (ELAC) threads by optimizing several crosslinking parameters. The results indicated that elevating the concentration of genipin to 2% and the solvent to 90% ethanol significantly enhanced the wet ultimate tensile stress of ELAC threads to 109MPa with a crosslinking degree of 65%. Furthermore, significantly higher adhesion and proliferation of hMSCs was observed in ELAC threads crosslinked with 2% genipin in 90% ethanol compared to 0.625% genipin in 1X PBS. In conclusion, ELAC threads with mechanical strength on par with native tendon have significant potential to be used as scaffolds in tendon tissue engineering applications.
[Display omitted]
► The mechanical properties of ELAC threads were similar to those of native tendon. ► Significant proliferation/adhesion of human stem cells on ELAC threads were evidenced. ► PBS solvents decreased the mechanical properties of ELAC threads. ► PBS solvents decreased the proliferation rate of human stem cells on ELAC threads.</description><subject>Alignment</subject><subject>Biomechanical Phenomena</subject><subject>Biomimetic Materials - chemistry</subject><subject>Biomimetic Materials - pharmacology</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Collagen - chemistry</subject><subject>Collagen - pharmacology</subject><subject>Collagens</subject><subject>Crosslinking</subject><subject>Electrochemically aligned collagen</subject><subject>Electrochemistry</subject><subject>Ethanol</subject><subject>Ethanol - chemistry</subject><subject>Ethyl alcohol</subject><subject>Genipin</subject><subject>Human mesenchymal stem cell mechanical properties</subject><subject>Humans</subject><subject>Iridoids - chemistry</subject><subject>Mechanical Phenomena</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - drug effects</subject><subject>Strength</subject><subject>Tendons</subject><subject>Time Factors</subject><subject>Tissue engineering</subject><issn>1751-6161</issn><issn>1878-0180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFv1DAQhS0EoqXwC5BQjlwSxnacOAcOqCqlUiUucLYce7LrxbEX21up_x7vbuGIenqW_L2Z0XuEvKfQUaDDp123W-d57RhQ1sHQVXlBLqkcZQtUwsv6HgVtBzrQC_Im5x3AACDla3LBOHDW8_GSbG8xuL0LjUkxZ-_CLxc2DXp80AVzU7bY5JIwbMq2icvxw5QUzRZXZ7T3j432bhPQNiZ6rzcYmhJPrjoB_dFSMNgY8lvyatE-47snvSI_v978uP7W3n-_vbv-ct-aXsjSou7lIsHAZBm11srZzpQtdGCC90xzSSfOpBZ20aOEGoO0bLEcR8EFLrPkV-Tjee4-xd8HzEWtLhusxwWMh6yo4DCNk5iegTIGYoSe9xXlZ_QUU8JF7ZNbdXpUFNSxDbVTpzbUsQ0Fg6pSXR-eFhzmFe0_z9_4K_D5DGBN5MFhUtk4DAatSzVoZaP774I_xjCdDw</recordid><startdate>20121101</startdate><enddate>20121101</enddate><creator>Alfredo Uquillas, Jorge</creator><creator>Kishore, Vipuil</creator><creator>Akkus, Ozan</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7TB</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>JG9</scope></search><sort><creationdate>20121101</creationdate><title>Genipin crosslinking elevates the strength of electrochemically aligned collagen to the level of tendons</title><author>Alfredo Uquillas, Jorge ; Kishore, Vipuil ; Akkus, Ozan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-ea48f80c09d21ddd8bdb12f1625342a3819328a5dfa7800168d2fd3e7535efb83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Alignment</topic><topic>Biomechanical Phenomena</topic><topic>Biomimetic Materials - chemistry</topic><topic>Biomimetic Materials - pharmacology</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Collagen - chemistry</topic><topic>Collagen - pharmacology</topic><topic>Collagens</topic><topic>Crosslinking</topic><topic>Electrochemically aligned collagen</topic><topic>Electrochemistry</topic><topic>Ethanol</topic><topic>Ethanol - chemistry</topic><topic>Ethyl alcohol</topic><topic>Genipin</topic><topic>Human mesenchymal stem cell mechanical properties</topic><topic>Humans</topic><topic>Iridoids - chemistry</topic><topic>Mechanical Phenomena</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - drug effects</topic><topic>Strength</topic><topic>Tendons</topic><topic>Time Factors</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alfredo Uquillas, Jorge</creatorcontrib><creatorcontrib>Kishore, Vipuil</creatorcontrib><creatorcontrib>Akkus, Ozan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><jtitle>Journal of the mechanical behavior of biomedical materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alfredo Uquillas, Jorge</au><au>Kishore, Vipuil</au><au>Akkus, Ozan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genipin crosslinking elevates the strength of electrochemically aligned collagen to the level of tendons</atitle><jtitle>Journal of the mechanical behavior of biomedical materials</jtitle><addtitle>J Mech Behav Biomed Mater</addtitle><date>2012-11-01</date><risdate>2012</risdate><volume>15</volume><spage>176</spage><epage>189</epage><pages>176-189</pages><issn>1751-6161</issn><eissn>1878-0180</eissn><abstract>Collagen-based tissue mimics are important in clinical research because collagen is the main structural element in tendons. The current study aimed to improve the mechanical strength of Electronically Aligned Collagen (ELAC) threads by optimizing several crosslinking parameters. The results indicated that elevating the concentration of genipin to 2% and the solvent to 90% ethanol significantly enhanced the wet ultimate tensile stress of ELAC threads to 109MPa with a crosslinking degree of 65%. Furthermore, significantly higher adhesion and proliferation of hMSCs was observed in ELAC threads crosslinked with 2% genipin in 90% ethanol compared to 0.625% genipin in 1X PBS. In conclusion, ELAC threads with mechanical strength on par with native tendon have significant potential to be used as scaffolds in tendon tissue engineering applications.
[Display omitted]
► The mechanical properties of ELAC threads were similar to those of native tendon. ► Significant proliferation/adhesion of human stem cells on ELAC threads were evidenced. ► PBS solvents decreased the mechanical properties of ELAC threads. ► PBS solvents decreased the proliferation rate of human stem cells on ELAC threads.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>23032437</pmid><doi>10.1016/j.jmbbm.2012.06.012</doi><tpages>14</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Alignment Biomechanical Phenomena Biomimetic Materials - chemistry Biomimetic Materials - pharmacology Cell Adhesion - drug effects Cell Proliferation - drug effects Collagen - chemistry Collagen - pharmacology Collagens Crosslinking Electrochemically aligned collagen Electrochemistry Ethanol Ethanol - chemistry Ethyl alcohol Genipin Human mesenchymal stem cell mechanical properties Humans Iridoids - chemistry Mechanical Phenomena Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - drug effects Strength Tendons Time Factors Tissue engineering |
| title | Genipin crosslinking elevates the strength of electrochemically aligned collagen to the level of tendons |
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