Xenobiotic oxidation by cytochrome P-450-enriched extracts of streptomyces griseus

Crude extracts Streptomyces griseus grown on soybean flour-enriched medium contain high levels of cytochrome P-450. The cytochrome P-450-enriched fractions, obtained by ammonium sulfate fractionation (30–50% saturation), catalyze the NADPH-dependent oxidation of a variety of xenobiotics when complem...

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Veröffentlicht in:	Biochemical and biophysical research communications 1988-12, Vol.157 (3), p.1417-1422
Hauptverfasser:	Trower, Michael K., Sariaslani, F. Sima, Kitson, Fulton G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonium Sulfate Analytical, structural and metabolic biochemistry Aniline Compounds - metabolism Benzene - metabolism Benzopyrans - metabolism Biological and medical sciences Chemical Phenomena Chemistry Coumarins - metabolism Cyclohexanes - metabolism Cytochrome P-450 Enzyme System - isolation & purification Cytochrome P-450 Enzyme System - metabolism Enzymes and enzyme inhibitors Estradiol - metabolism Ferredoxin-NADP Reductase - metabolism Ferredoxins - metabolism Fractional Precipitation Fundamental and applied biological sciences. Psychology Kinetics NADP - pharmacology Oxidation-Reduction Oxidoreductases Pyridines - metabolism Streptomyces griseus - enzymology Substrate Specificity Toluene - metabolism Xenobiotics - metabolism
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