Phosphatidylinositol kinase activity of a plasma membrane-associated calcium-activated protein kinase from pea

Phosphatidylinositol kinase activity of a plasma membrane-associated calcium-activated protein kinase from pea

A purified protein kinase preparation from pea is shown to contain phosphatidylinositol kinase activity. The preparation, produced by membrane solubilisation and ATP-agarose affinity chromatography, contains proteins of 18 kDa (ppl8, a calcium activated autophosphorylating protein-serine kinase), 67...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:FEBS letters 1988-09, Vol.238 (1), p.87-89
Hauptverfasser: Blowers, D.P., Trewavas, A.J.
Format: Artikel
Sprache:eng
Schlagworte:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Lipid kinase
microsomes
Phosphatidylinositol phosphate
Phosphatidylinositol phosphorylation
PI, phosphatidylinositol
PIP, phosphatidylinositol monophosphate
PIP2, phosphatidylinositol bisphosphate
Pisum sativum
protein kinase
Transferases
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A purified protein kinase preparation from pea is shown to contain phosphatidylinositol kinase activity. The preparation, produced by membrane solubilisation and ATP-agarose affinity chromatography, contains proteins of 18 kDa (ppl8, a calcium activated autophosphorylating protein-serine kinase), 67 kDa and 48 kDa and exhibits the phosphorylation of an endogenous lipid when supplied with [γ- 32P]ATP. This product is tentatively identified as lysophosphatidylinositol phosphate. When supplied with [γ- 32P]ATP and exogenous phosphatidylinositol, the production of phosphatidylinositol phosphate is observed.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(88)80231-X