Vitellogenin mRNA from Dacus oleae: characterization, cDNA cloning, and dependence on sex and developmental stage

Poly(A)+ RNA was isolated from 5‐day‐old females of the olive fruitfly Dacus oleae. In vitro translation of mRNA, followed by immunoprecipitation with anti‐vitellin antiserum, demonstrated the synthesis of two vitellogenin polypeptides of 54,000 and 56,000 daltons. In sucrose gradient centrifugation...

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Veröffentlicht in:	Archives of insect biochemistry and physiology 1988, Vol.9 (2), p.119-134
Hauptverfasser:	Levedakou, E.N, Konialis, C.P, Sekeris, C.E
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN ARN BIOCHEMISTRY Biochemistry. Physiology. Immunology BIOCHIMIE Biological and medical sciences BIOQUIMICA CLONACION CLONAGE CLONING DACUS Dacus oleae Diptera DNA Fundamental and applied biological sciences. Psychology GENE GENES Insecta Invertebrates ISOLATION TECHNIQUE male vitellogenin OVA OVULE OVULO PESTS OF PLANTS Physiology. Development PLAGAS DE PLANTAS PROTEINAS PROTEINE PROTEINS RAVAGEUR DES PLANTES RNA TECHNIQUE DE L'ISOLEMENT TECNICAS DE AISLAMIENTO Tephritidae VITELLIN VITELLOGENESIS yolk protein genes
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