Identification of a gene linked to Rhizobium meliloti ntrA whose product is homologous to a family to ATP-binding proteins
The ntrA gene of Rhizobium meliloti has recently been identified and shown to be required for a diverse set of metabolic functions (C. W. Ronson, B. T. Nixon, L. M. Albright, and F. M. Ausubel, J. Bacteriol. 169:2424-2431, 1987). As a result of sequencing the ntrA gene and its flanking regions from...
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| Veröffentlicht in: | Journal of Bacteriology 1989-04, Vol.171 (4), p.1932-1941 |
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| container_end_page | 1941 |
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| container_issue | 4 |
| container_start_page | 1932 |
| container_title | Journal of Bacteriology |
| container_volume | 171 |
| creator | Albright, L.M Ronson, C.W Nixon, B.T Ausubel, F.M |
| description | The ntrA gene of Rhizobium meliloti has recently been identified and shown to be required for a diverse set of metabolic functions (C. W. Ronson, B. T. Nixon, L. M. Albright, and F. M. Ausubel, J. Bacteriol. 169:2424-2431, 1987). As a result of sequencing the ntrA gene and its flanking regions from R. meliloti, we identified an open reading frame directly upstream of ntrA, ORF1, whose predicted product is homologous to a superfamily of ATP-binding proteins involved in transport, cell division, nodulation, and DNA repair. The homology of ORF1 to this superfamily and its proximity to ntrA led us to investigate its role in symbiosis by mutagenesis and expression studies. We were unable to isolate an insertion mutation in ORF1, suggesting that ORF1 may code for an essential function. We identified the start of transcription for the ntrA gene in vegetative cells and bacteroids and showed that ORF1 and ntrA are transcriptionally unlinked. ORF1 appears to be in an operon with one or more upstream genes |
| doi_str_mv | 10.1128/jb.171.4.1932-1941.1989 |
| format | Article |
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W. Ronson, B. T. Nixon, L. M. Albright, and F. M. Ausubel, J. Bacteriol. 169:2424-2431, 1987). As a result of sequencing the ntrA gene and its flanking regions from R. meliloti, we identified an open reading frame directly upstream of ntrA, ORF1, whose predicted product is homologous to a superfamily of ATP-binding proteins involved in transport, cell division, nodulation, and DNA repair. The homology of ORF1 to this superfamily and its proximity to ntrA led us to investigate its role in symbiosis by mutagenesis and expression studies. We were unable to isolate an insertion mutation in ORF1, suggesting that ORF1 may code for an essential function. We identified the start of transcription for the ntrA gene in vegetative cells and bacteroids and showed that ORF1 and ntrA are transcriptionally unlinked. ORF1 appears to be in an operon with one or more upstream genes</description><identifier>ISSN: 0021-9193</identifier><identifier>EISSN: 1098-5530</identifier><identifier>EISSN: 1067-8832</identifier><identifier>DOI: 10.1128/jb.171.4.1932-1941.1989</identifier><identifier>PMID: 2703463</identifier><identifier>CODEN: JOBAAY</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>ADENOSINE TRIPHOSPHATE ; ADENOSINTRIFOSFATO ; Amino Acid Sequence ; Bacterial Proteins - genetics ; Bacteriology ; Base Sequence ; Biological and medical sciences ; Carrier Proteins - genetics ; Chromosome Mapping ; Codon ; DNA Mutational Analysis ; Fundamental and applied biological sciences. Psychology ; GENE ; GENES ; Genetics ; Microbiology ; Molecular Sequence Data ; Multigene Family ; NUCLEOTIDE ; NUCLEOTIDOS ; Peptide Chain Initiation, Translational ; PROTEINAS ; PROTEINE ; RHIZOBIUM ; Rhizobium - genetics ; SIMBIOSIS ; SYMBIOSE ; Transcription, Genetic</subject><ispartof>Journal of Bacteriology, 1989-04, Vol.171 (4), p.1932-1941</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c516t-ffd3ac853e04ec2e9a737b834185ae8ddd1e8cc8fb1773d4606338e02fe375e03</citedby><cites>FETCH-LOGICAL-c516t-ffd3ac853e04ec2e9a737b834185ae8ddd1e8cc8fb1773d4606338e02fe375e03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC209842/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC209842/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7346607$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2703463$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Albright, L.M</creatorcontrib><creatorcontrib>Ronson, C.W</creatorcontrib><creatorcontrib>Nixon, B.T</creatorcontrib><creatorcontrib>Ausubel, F.M</creatorcontrib><title>Identification of a gene linked to Rhizobium meliloti ntrA whose product is homologous to a family to ATP-binding proteins</title><title>Journal of Bacteriology</title><addtitle>J Bacteriol</addtitle><description>The ntrA gene of Rhizobium meliloti has recently been identified and shown to be required for a diverse set of metabolic functions (C. W. Ronson, B. T. Nixon, L. M. Albright, and F. M. Ausubel, J. Bacteriol. 169:2424-2431, 1987). As a result of sequencing the ntrA gene and its flanking regions from R. meliloti, we identified an open reading frame directly upstream of ntrA, ORF1, whose predicted product is homologous to a superfamily of ATP-binding proteins involved in transport, cell division, nodulation, and DNA repair. The homology of ORF1 to this superfamily and its proximity to ntrA led us to investigate its role in symbiosis by mutagenesis and expression studies. We were unable to isolate an insertion mutation in ORF1, suggesting that ORF1 may code for an essential function. We identified the start of transcription for the ntrA gene in vegetative cells and bacteroids and showed that ORF1 and ntrA are transcriptionally unlinked. ORF1 appears to be in an operon with one or more upstream genes</description><subject>ADENOSINE TRIPHOSPHATE</subject><subject>ADENOSINTRIFOSFATO</subject><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - genetics</subject><subject>Chromosome Mapping</subject><subject>Codon</subject><subject>DNA Mutational Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENES</subject><subject>Genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>NUCLEOTIDE</subject><subject>NUCLEOTIDOS</subject><subject>Peptide Chain Initiation, Translational</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>RHIZOBIUM</subject><subject>Rhizobium - genetics</subject><subject>SIMBIOSIS</subject><subject>SYMBIOSE</subject><subject>Transcription, Genetic</subject><issn>0021-9193</issn><issn>1098-5530</issn><issn>1067-8832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkUFv1DAQhSMEKkvhDyAhjIR6y2LHTuwcOKwqKJUqgaA9W449Trwk9mJnqdpfj6NdrejJI73vzXjmFcV7gteEVOLTtlsTTtZsTVpalaRlJFeifVasCG5FWdcUPy9WGFekbDPysniV0hZjwlhdnRVnFceUNXRVPF4b8LOzTqvZBY-CRQr14AGNzv8Gg-aAfg7uMXRuP6EJRjeG2SE_xw26H0ICtIvB7PWMXEJDmMIY-rBPi00hqyY3Piz15vZH2TlvnO8XwwzOp9fFC6vGBG-O73lx9_XL7eW38ub71fXl5qbUNWnm0lpDlRY1BcxAV9AqTnknKCOiViCMMQSE1sJ2hHNqWIMbSgXgygLlNWB6Xnw-9N3tuwmMzvtGNcpddJOKDzIoJ58q3g2yD39llS_Jquy_OPpj-LOHNMvJJQ3jqDzkVSXJt8ZUkAzyA6hjSCmCPc0gWC6pyW0nc2qSySU1uaQml9Sy893_Xzz5jjFl_eNRV0mr0UbltUsnjGeowTxjHw7Y4Prh3kWQKk1Ph2bm7YGxKkjVx9zm7pdoq4bzmv4DPaa2uw</recordid><startdate>19890401</startdate><enddate>19890401</enddate><creator>Albright, L.M</creator><creator>Ronson, C.W</creator><creator>Nixon, B.T</creator><creator>Ausubel, F.M</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>5PM</scope></search><sort><creationdate>19890401</creationdate><title>Identification of a gene linked to Rhizobium meliloti ntrA whose product is homologous to a family to ATP-binding proteins</title><author>Albright, L.M ; Ronson, C.W ; Nixon, B.T ; Ausubel, F.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-ffd3ac853e04ec2e9a737b834185ae8ddd1e8cc8fb1773d4606338e02fe375e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>ADENOSINE TRIPHOSPHATE</topic><topic>ADENOSINTRIFOSFATO</topic><topic>Amino Acid Sequence</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins - genetics</topic><topic>Chromosome Mapping</topic><topic>Codon</topic><topic>DNA Mutational Analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENES</topic><topic>Genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>NUCLEOTIDE</topic><topic>NUCLEOTIDOS</topic><topic>Peptide Chain Initiation, Translational</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>RHIZOBIUM</topic><topic>Rhizobium - genetics</topic><topic>SIMBIOSIS</topic><topic>SYMBIOSE</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Albright, L.M</creatorcontrib><creatorcontrib>Ronson, C.W</creatorcontrib><creatorcontrib>Nixon, B.T</creatorcontrib><creatorcontrib>Ausubel, F.M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Bacteriology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Albright, L.M</au><au>Ronson, C.W</au><au>Nixon, B.T</au><au>Ausubel, F.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a gene linked to Rhizobium meliloti ntrA whose product is homologous to a family to ATP-binding proteins</atitle><jtitle>Journal of Bacteriology</jtitle><addtitle>J Bacteriol</addtitle><date>1989-04-01</date><risdate>1989</risdate><volume>171</volume><issue>4</issue><spage>1932</spage><epage>1941</epage><pages>1932-1941</pages><issn>0021-9193</issn><eissn>1098-5530</eissn><eissn>1067-8832</eissn><coden>JOBAAY</coden><abstract>The ntrA gene of Rhizobium meliloti has recently been identified and shown to be required for a diverse set of metabolic functions (C. W. Ronson, B. T. Nixon, L. M. Albright, and F. M. Ausubel, J. Bacteriol. 169:2424-2431, 1987). As a result of sequencing the ntrA gene and its flanking regions from R. meliloti, we identified an open reading frame directly upstream of ntrA, ORF1, whose predicted product is homologous to a superfamily of ATP-binding proteins involved in transport, cell division, nodulation, and DNA repair. The homology of ORF1 to this superfamily and its proximity to ntrA led us to investigate its role in symbiosis by mutagenesis and expression studies. We were unable to isolate an insertion mutation in ORF1, suggesting that ORF1 may code for an essential function. We identified the start of transcription for the ntrA gene in vegetative cells and bacteroids and showed that ORF1 and ntrA are transcriptionally unlinked. ORF1 appears to be in an operon with one or more upstream genes</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>2703463</pmid><doi>10.1128/jb.171.4.1932-1941.1989</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | ADENOSINE TRIPHOSPHATE ADENOSINTRIFOSFATO Amino Acid Sequence Bacterial Proteins - genetics Bacteriology Base Sequence Biological and medical sciences Carrier Proteins - genetics Chromosome Mapping Codon DNA Mutational Analysis Fundamental and applied biological sciences. Psychology GENE GENES Genetics Microbiology Molecular Sequence Data Multigene Family NUCLEOTIDE NUCLEOTIDOS Peptide Chain Initiation, Translational PROTEINAS PROTEINE RHIZOBIUM Rhizobium - genetics SIMBIOSIS SYMBIOSE Transcription, Genetic |
| title | Identification of a gene linked to Rhizobium meliloti ntrA whose product is homologous to a family to ATP-binding proteins |
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