Swertiamarin ameliorates inflammation and osteoclastogenesis intermediates in IL-1β induced rat fibroblast-like synoviocytes
Objective and design Rheumatoid arthritis is a chronic inflammatory and autoimmune disease that leads to aggressive joint cartilage and bone destruction. Swertiamarin is a secoiridoid glycoside found in Enicostema axillare (Lam) A. Raynal, a medicinal plant used in the Indian system of traditional m...
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| Veröffentlicht in: | Inflammation research 2014-06, Vol.63 (6), p.451-462 |
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| creator | Saravanan, S. Hairul Islam, V. I. Thirugnanasambantham, K. Pazhanivel, N. Raghuraman, N. Gabriel Paulraj, M. Ignacimuthu, S. |
| description | Objective and design
Rheumatoid arthritis is a chronic inflammatory and autoimmune disease that leads to aggressive joint cartilage and bone destruction. Swertiamarin is a secoiridoid glycoside found in
Enicostema axillare
(Lam) A. Raynal, a medicinal plant used in the Indian system of traditional medicine. In the present study, the potential of swertiamarin was evaluated in IL-1β induced fibroblast-like synoviocytes (FLS).
Methods
The FLS were isolated from Freund’s Complete Adjuvant induced arthritic (AA) rats and cultured with IL-1β. The normal FLS and AA-FLS were cultured and used for subsequent experiment in fibroblastic morphology form. The efficacy of swertiamarin (10–50 μg/ml) was evaluated on mRNA and protein expression levels of inflammatory and osteoclastogenesis mediators. The efficacy was also evaluated on p38 MAPKα levels with time course studies (2, 4, 6, 8 and 12 h).
Results
IL-1β induced cell proliferation (149.46 ± 13.73 %) and NO production (162.03 ± 11.03 %) in AA-FLS; treatment with swertiamarin controlled proliferation (82.77 ± 4.22 %) and NO production (82.06 ± 3.91 % at 50 μg/ml) in a dose-dependent manner. It also significantly (
P
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| doi_str_mv | 10.1007/s00011-014-0717-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1529934734</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1524342034</sourcerecordid><originalsourceid>FETCH-LOGICAL-c377t-afce9fd51f194d41d611de5cd3c273646de5214efb977abfbcd648db1933ed513</originalsourceid><addsrcrecordid>eNqNkbFuFDEQhq0IlIQkD5AGbUlj8Nje87pEEQmRTqJIkOgsrz2OnOyug70LuoKXyoPkmfBxByVKNTOa7_-L_yfkHNh7YEx9KIwxAMpAUqZA0faAHIPkjGrWfXtVd8YFFZ1gR-RNKfeV7njHD8kRl1Jz3cIx-XXzE_Mc7WhznBo74hBTtjOWJk5hsONo55jqY_JNKjMmN9gypzucsMQtM2Me0ce9orleU3h-qptfHPqmOjUh9jn1Wxkd4gM2ZTOlHzG5TZWcktfBDgXP9vOEfL38dHvxma6_XF1ffFxTJ5SaqQ0OdfAtBNDSS_ArAI-t88JxJVZyVQ8OEkOvlbJ96J1fyc73oIXAKhMn5N3O9zGn7wuW2YyxOBwGO2FaioGWay2kEvIlqBQ14z8o7FCXUykZg3nMsQa5McDMtiCzK8jUgsy2INNWzdu9_dLX4P4p_jZSAb4DSn1Nd5jNfVryVNP5j-tv0QCfeQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1524342034</pqid></control><display><type>article</type><title>Swertiamarin ameliorates inflammation and osteoclastogenesis intermediates in IL-1β induced rat fibroblast-like synoviocytes</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Saravanan, S. ; Hairul Islam, V. I. ; Thirugnanasambantham, K. ; Pazhanivel, N. ; Raghuraman, N. ; Gabriel Paulraj, M. ; Ignacimuthu, S.</creator><creatorcontrib>Saravanan, S. ; Hairul Islam, V. I. ; Thirugnanasambantham, K. ; Pazhanivel, N. ; Raghuraman, N. ; Gabriel Paulraj, M. ; Ignacimuthu, S.</creatorcontrib><description>Objective and design
Rheumatoid arthritis is a chronic inflammatory and autoimmune disease that leads to aggressive joint cartilage and bone destruction. Swertiamarin is a secoiridoid glycoside found in
Enicostema axillare
(Lam) A. Raynal, a medicinal plant used in the Indian system of traditional medicine. In the present study, the potential of swertiamarin was evaluated in IL-1β induced fibroblast-like synoviocytes (FLS).
Methods
The FLS were isolated from Freund’s Complete Adjuvant induced arthritic (AA) rats and cultured with IL-1β. The normal FLS and AA-FLS were cultured and used for subsequent experiment in fibroblastic morphology form. The efficacy of swertiamarin (10–50 μg/ml) was evaluated on mRNA and protein expression levels of inflammatory and osteoclastogenesis mediators. The efficacy was also evaluated on p38 MAPKα levels with time course studies (2, 4, 6, 8 and 12 h).
Results
IL-1β induced cell proliferation (149.46 ± 13.73 %) and NO production (162.03 ± 11.03 %) in AA-FLS; treatment with swertiamarin controlled proliferation (82.77 ± 4.22 %) and NO production (82.06 ± 3.91 % at 50 μg/ml) in a dose-dependent manner. It also significantly (
P
< 0.05) modulated the expression of apoptotic marker (caspase 3), proinflammation mediators (TNFα, IL-6, PGE2, COX-2, iNOS, MMPs) and osteoclastogenic mediator (RANKL) at both the mRNA and protein levels. Treatment with swertiamarin inhibited the levels of p38 MAPKα in a dose-dependent manner and also significantly (
P
< 0.05) attenuated the release of the same in time dependent mode.
Conclusion
These findings suggest that treatment with swertiamarin attenuated IL-1β induced FLS, and it revealed anti-inflammatory potential by attenuating aggressive FLS.</description><identifier>ISSN: 1023-3830</identifier><identifier>EISSN: 1420-908X</identifier><identifier>DOI: 10.1007/s00011-014-0717-5</identifier><identifier>PMID: 24492951</identifier><language>eng</language><publisher>Basel: Springer Basel</publisher><subject>Allergology ; Animals ; Anti-Inflammatory Agents - pharmacology ; Apoptosis - drug effects ; Arthritis, Experimental - immunology ; Arthritis, Rheumatoid - immunology ; Biomedical and Life Sciences ; Biomedicine ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; Cytokines - genetics ; Cytokines - immunology ; Dermatology ; Enicostema ; Fibroblasts - cytology ; Fibroblasts - drug effects ; Fibroblasts - immunology ; Immunology ; Iridoid Glucosides - pharmacology ; Mice, Inbred C57BL ; Neurology ; Nitric Oxide - immunology ; Original Research Paper ; Osteoclasts - cytology ; p38 Mitogen-Activated Protein Kinases - immunology ; Pharmacology/Toxicology ; Pyrones - pharmacology ; RANK Ligand - immunology ; Rats, Sprague-Dawley ; Rheumatology ; Synovial Membrane - cytology</subject><ispartof>Inflammation research, 2014-06, Vol.63 (6), p.451-462</ispartof><rights>Springer Basel 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-afce9fd51f194d41d611de5cd3c273646de5214efb977abfbcd648db1933ed513</citedby><cites>FETCH-LOGICAL-c377t-afce9fd51f194d41d611de5cd3c273646de5214efb977abfbcd648db1933ed513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00011-014-0717-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00011-014-0717-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24492951$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saravanan, S.</creatorcontrib><creatorcontrib>Hairul Islam, V. I.</creatorcontrib><creatorcontrib>Thirugnanasambantham, K.</creatorcontrib><creatorcontrib>Pazhanivel, N.</creatorcontrib><creatorcontrib>Raghuraman, N.</creatorcontrib><creatorcontrib>Gabriel Paulraj, M.</creatorcontrib><creatorcontrib>Ignacimuthu, S.</creatorcontrib><title>Swertiamarin ameliorates inflammation and osteoclastogenesis intermediates in IL-1β induced rat fibroblast-like synoviocytes</title><title>Inflammation research</title><addtitle>Inflamm. Res</addtitle><addtitle>Inflamm Res</addtitle><description>Objective and design
Rheumatoid arthritis is a chronic inflammatory and autoimmune disease that leads to aggressive joint cartilage and bone destruction. Swertiamarin is a secoiridoid glycoside found in
Enicostema axillare
(Lam) A. Raynal, a medicinal plant used in the Indian system of traditional medicine. In the present study, the potential of swertiamarin was evaluated in IL-1β induced fibroblast-like synoviocytes (FLS).
Methods
The FLS were isolated from Freund’s Complete Adjuvant induced arthritic (AA) rats and cultured with IL-1β. The normal FLS and AA-FLS were cultured and used for subsequent experiment in fibroblastic morphology form. The efficacy of swertiamarin (10–50 μg/ml) was evaluated on mRNA and protein expression levels of inflammatory and osteoclastogenesis mediators. The efficacy was also evaluated on p38 MAPKα levels with time course studies (2, 4, 6, 8 and 12 h).
Results
IL-1β induced cell proliferation (149.46 ± 13.73 %) and NO production (162.03 ± 11.03 %) in AA-FLS; treatment with swertiamarin controlled proliferation (82.77 ± 4.22 %) and NO production (82.06 ± 3.91 % at 50 μg/ml) in a dose-dependent manner. It also significantly (
P
< 0.05) modulated the expression of apoptotic marker (caspase 3), proinflammation mediators (TNFα, IL-6, PGE2, COX-2, iNOS, MMPs) and osteoclastogenic mediator (RANKL) at both the mRNA and protein levels. Treatment with swertiamarin inhibited the levels of p38 MAPKα in a dose-dependent manner and also significantly (
P
< 0.05) attenuated the release of the same in time dependent mode.
Conclusion
These findings suggest that treatment with swertiamarin attenuated IL-1β induced FLS, and it revealed anti-inflammatory potential by attenuating aggressive FLS.</description><subject>Allergology</subject><subject>Animals</subject><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>Apoptosis - drug effects</subject><subject>Arthritis, Experimental - immunology</subject><subject>Arthritis, Rheumatoid - immunology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Cytokines - genetics</subject><subject>Cytokines - immunology</subject><subject>Dermatology</subject><subject>Enicostema</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - immunology</subject><subject>Immunology</subject><subject>Iridoid Glucosides - pharmacology</subject><subject>Mice, Inbred C57BL</subject><subject>Neurology</subject><subject>Nitric Oxide - immunology</subject><subject>Original Research Paper</subject><subject>Osteoclasts - cytology</subject><subject>p38 Mitogen-Activated Protein Kinases - immunology</subject><subject>Pharmacology/Toxicology</subject><subject>Pyrones - pharmacology</subject><subject>RANK Ligand - immunology</subject><subject>Rats, Sprague-Dawley</subject><subject>Rheumatology</subject><subject>Synovial Membrane - cytology</subject><issn>1023-3830</issn><issn>1420-908X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkbFuFDEQhq0IlIQkD5AGbUlj8Nje87pEEQmRTqJIkOgsrz2OnOyug70LuoKXyoPkmfBxByVKNTOa7_-L_yfkHNh7YEx9KIwxAMpAUqZA0faAHIPkjGrWfXtVd8YFFZ1gR-RNKfeV7njHD8kRl1Jz3cIx-XXzE_Mc7WhznBo74hBTtjOWJk5hsONo55jqY_JNKjMmN9gypzucsMQtM2Me0ce9orleU3h-qptfHPqmOjUh9jn1Wxkd4gM2ZTOlHzG5TZWcktfBDgXP9vOEfL38dHvxma6_XF1ffFxTJ5SaqQ0OdfAtBNDSS_ArAI-t88JxJVZyVQ8OEkOvlbJ96J1fyc73oIXAKhMn5N3O9zGn7wuW2YyxOBwGO2FaioGWay2kEvIlqBQ14z8o7FCXUykZg3nMsQa5McDMtiCzK8jUgsy2INNWzdu9_dLX4P4p_jZSAb4DSn1Nd5jNfVryVNP5j-tv0QCfeQ</recordid><startdate>20140601</startdate><enddate>20140601</enddate><creator>Saravanan, S.</creator><creator>Hairul Islam, V. I.</creator><creator>Thirugnanasambantham, K.</creator><creator>Pazhanivel, N.</creator><creator>Raghuraman, N.</creator><creator>Gabriel Paulraj, M.</creator><creator>Ignacimuthu, S.</creator><general>Springer Basel</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>20140601</creationdate><title>Swertiamarin ameliorates inflammation and osteoclastogenesis intermediates in IL-1β induced rat fibroblast-like synoviocytes</title><author>Saravanan, S. ; Hairul Islam, V. I. ; Thirugnanasambantham, K. ; Pazhanivel, N. ; Raghuraman, N. ; Gabriel Paulraj, M. ; Ignacimuthu, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-afce9fd51f194d41d611de5cd3c273646de5214efb977abfbcd648db1933ed513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Allergology</topic><topic>Animals</topic><topic>Anti-Inflammatory Agents - pharmacology</topic><topic>Apoptosis - drug effects</topic><topic>Arthritis, Experimental - immunology</topic><topic>Arthritis, Rheumatoid - immunology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Cytokines - genetics</topic><topic>Cytokines - immunology</topic><topic>Dermatology</topic><topic>Enicostema</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - immunology</topic><topic>Immunology</topic><topic>Iridoid Glucosides - pharmacology</topic><topic>Mice, Inbred C57BL</topic><topic>Neurology</topic><topic>Nitric Oxide - immunology</topic><topic>Original Research Paper</topic><topic>Osteoclasts - cytology</topic><topic>p38 Mitogen-Activated Protein Kinases - immunology</topic><topic>Pharmacology/Toxicology</topic><topic>Pyrones - pharmacology</topic><topic>RANK Ligand - immunology</topic><topic>Rats, Sprague-Dawley</topic><topic>Rheumatology</topic><topic>Synovial Membrane - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saravanan, S.</creatorcontrib><creatorcontrib>Hairul Islam, V. I.</creatorcontrib><creatorcontrib>Thirugnanasambantham, K.</creatorcontrib><creatorcontrib>Pazhanivel, N.</creatorcontrib><creatorcontrib>Raghuraman, N.</creatorcontrib><creatorcontrib>Gabriel Paulraj, M.</creatorcontrib><creatorcontrib>Ignacimuthu, S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Inflammation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saravanan, S.</au><au>Hairul Islam, V. I.</au><au>Thirugnanasambantham, K.</au><au>Pazhanivel, N.</au><au>Raghuraman, N.</au><au>Gabriel Paulraj, M.</au><au>Ignacimuthu, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Swertiamarin ameliorates inflammation and osteoclastogenesis intermediates in IL-1β induced rat fibroblast-like synoviocytes</atitle><jtitle>Inflammation research</jtitle><stitle>Inflamm. Res</stitle><addtitle>Inflamm Res</addtitle><date>2014-06-01</date><risdate>2014</risdate><volume>63</volume><issue>6</issue><spage>451</spage><epage>462</epage><pages>451-462</pages><issn>1023-3830</issn><eissn>1420-908X</eissn><abstract>Objective and design
Rheumatoid arthritis is a chronic inflammatory and autoimmune disease that leads to aggressive joint cartilage and bone destruction. Swertiamarin is a secoiridoid glycoside found in
Enicostema axillare
(Lam) A. Raynal, a medicinal plant used in the Indian system of traditional medicine. In the present study, the potential of swertiamarin was evaluated in IL-1β induced fibroblast-like synoviocytes (FLS).
Methods
The FLS were isolated from Freund’s Complete Adjuvant induced arthritic (AA) rats and cultured with IL-1β. The normal FLS and AA-FLS were cultured and used for subsequent experiment in fibroblastic morphology form. The efficacy of swertiamarin (10–50 μg/ml) was evaluated on mRNA and protein expression levels of inflammatory and osteoclastogenesis mediators. The efficacy was also evaluated on p38 MAPKα levels with time course studies (2, 4, 6, 8 and 12 h).
Results
IL-1β induced cell proliferation (149.46 ± 13.73 %) and NO production (162.03 ± 11.03 %) in AA-FLS; treatment with swertiamarin controlled proliferation (82.77 ± 4.22 %) and NO production (82.06 ± 3.91 % at 50 μg/ml) in a dose-dependent manner. It also significantly (
P
< 0.05) modulated the expression of apoptotic marker (caspase 3), proinflammation mediators (TNFα, IL-6, PGE2, COX-2, iNOS, MMPs) and osteoclastogenic mediator (RANKL) at both the mRNA and protein levels. Treatment with swertiamarin inhibited the levels of p38 MAPKα in a dose-dependent manner and also significantly (
P
< 0.05) attenuated the release of the same in time dependent mode.
Conclusion
These findings suggest that treatment with swertiamarin attenuated IL-1β induced FLS, and it revealed anti-inflammatory potential by attenuating aggressive FLS.</abstract><cop>Basel</cop><pub>Springer Basel</pub><pmid>24492951</pmid><doi>10.1007/s00011-014-0717-5</doi><tpages>12</tpages></addata></record> |
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| subjects | Allergology Animals Anti-Inflammatory Agents - pharmacology Apoptosis - drug effects Arthritis, Experimental - immunology Arthritis, Rheumatoid - immunology Biomedical and Life Sciences Biomedicine Cell Proliferation - drug effects Cell Survival - drug effects Cells, Cultured Cytokines - genetics Cytokines - immunology Dermatology Enicostema Fibroblasts - cytology Fibroblasts - drug effects Fibroblasts - immunology Immunology Iridoid Glucosides - pharmacology Mice, Inbred C57BL Neurology Nitric Oxide - immunology Original Research Paper Osteoclasts - cytology p38 Mitogen-Activated Protein Kinases - immunology Pharmacology/Toxicology Pyrones - pharmacology RANK Ligand - immunology Rats, Sprague-Dawley Rheumatology Synovial Membrane - cytology |
| title | Swertiamarin ameliorates inflammation and osteoclastogenesis intermediates in IL-1β induced rat fibroblast-like synoviocytes |
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