Biomarker discovery and proteomic evaluation of cadmium toxicity on a collembolan species, Paronychiurus kimi (Lee)
The goal of this study was to identify promising new biomarkers of cadmium by identifying differentially expressed proteins in Paronychiurus kimi after exposure to cadmium. Through proteomic analysis of P. kimi using 1‐D PAGE and nano‐LC‐MS/MS, 36 downregulated proteins and 40 upregulated proteins w...
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| Veröffentlicht in: | Proteomics (Weinheim) 2011-06, Vol.11 (11), p.2294-2307 |
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| creator | Son, Jino Lee, Sung-Eun Park, Byeoung-Soo Jung, Jinho Park, Hyung Soon Bang, Joo Young Kang, Gum-Yong Cho, Kijong |
| description | The goal of this study was to identify promising new biomarkers of cadmium by identifying differentially expressed proteins in Paronychiurus kimi after exposure to cadmium. Through proteomic analysis of P. kimi using 1‐D PAGE and nano‐LC‐MS/MS, 36 downregulated proteins and 40 upregulated proteins were found. Some of the downregulated and upregulated proteins were verified by LC‐MS/MS analysis after 2‐D PAGE. Downregulated proteins in response to cadmium exposure were involved in glycolysis and energy metabolism, chaperones, transcription, reproduction, and neuron growth. In contrast, proteins involved in glycolysis and energy production, neurogenesis, defense systems response to bacteria, and protein biosynthesis were upregulated in cadmium‐treated collembolans. Cubulin may be a potential biomarker for the detection of cadmium in P. kimi since this biomarker was able to low levels (3.5 mg/kg) of cadmium. The 14‐3‐3 ζ was also found to be a potential biomarker for the detection of medium levels (14 mg/kg) of cadmium. Collembolans may be an alternative tool to humans because many collembolans proteins show a high homology to human proteins. |
| doi_str_mv | 10.1002/pmic.200900690 |
| format | Article |
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Through proteomic analysis of P. kimi using 1‐D PAGE and nano‐LC‐MS/MS, 36 downregulated proteins and 40 upregulated proteins were found. Some of the downregulated and upregulated proteins were verified by LC‐MS/MS analysis after 2‐D PAGE. Downregulated proteins in response to cadmium exposure were involved in glycolysis and energy metabolism, chaperones, transcription, reproduction, and neuron growth. In contrast, proteins involved in glycolysis and energy production, neurogenesis, defense systems response to bacteria, and protein biosynthesis were upregulated in cadmium‐treated collembolans. Cubulin may be a potential biomarker for the detection of cadmium in P. kimi since this biomarker was able to low levels (3.5 mg/kg) of cadmium. The 14‐3‐3 ζ was also found to be a potential biomarker for the detection of medium levels (14 mg/kg) of cadmium. Collembolans may be an alternative tool to humans because many collembolans proteins show a high homology to human proteins.</description><identifier>ISSN: 1615-9853</identifier><identifier>EISSN: 1615-9861</identifier><identifier>DOI: 10.1002/pmic.200900690</identifier><identifier>PMID: 21548089</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>1-D PAGE ; 2-D PAGE ; Analysis of Variance ; Analytical, structural and metabolic biochemistry ; Animal proteomics ; Animals ; Arthropods - drug effects ; Arthropods - physiology ; Biological and medical sciences ; Biomarkers ; Biomarkers - analysis ; Biomarkers - chemistry ; Biosynthesis ; Cadmium ; Cadmium - toxicity ; Chromatography, Liquid ; Electrophoresis, Gel, Two-Dimensional ; Electrophoresis, Polyacrylamide Gel ; Fundamental and applied biological sciences. Psychology ; Insect Proteins - analysis ; Insect Proteins - chemistry ; Insect Proteins - classification ; Insect Proteins - metabolism ; LC-MS/MS ; Miscellaneous ; Proteins ; Proteome - chemistry ; Proteome - drug effects ; Proteomics - methods ; Receptors, Cell Surface ; Tandem Mass Spectrometry ; Toxicity</subject><ispartof>Proteomics (Weinheim), 2011-06, Vol.11 (11), p.2294-2307</ispartof><rights>Copyright © 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><rights>Copyright © 2011 WILEY-VCH Verlag GmbH & Co. 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Psychology</subject><subject>Insect Proteins - analysis</subject><subject>Insect Proteins - chemistry</subject><subject>Insect Proteins - classification</subject><subject>Insect Proteins - metabolism</subject><subject>LC-MS/MS</subject><subject>Miscellaneous</subject><subject>Proteins</subject><subject>Proteome - chemistry</subject><subject>Proteome - drug effects</subject><subject>Proteomics - methods</subject><subject>Receptors, Cell Surface</subject><subject>Tandem Mass Spectrometry</subject><subject>Toxicity</subject><issn>1615-9853</issn><issn>1615-9861</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxSMEoqVw5YgsIaQikcUfcRIf6RbaSlsoEghu1sSeCLdJvLWT0vz3uNrtUnHh5NHo955n5mXZS0YXjFL-ft07s-CUKkpLRR9l-6xkMld1yR7vain2smcxXlLKqlpVT7M9zmRR01rtZ_HI-R7CFQZiXTT-BsNMYLBkHfyIPrkTvIFugtH5gfiWGLC9m3oy-ltn3DiT1AZifNdh3_gOBhLXaBzGd-QCgh9m88tNYYrkyvWOHK4Q3z7PnrTQRXyxfQ-y758-flue5qsvJ2fLD6vcSKFobhorBHLBuQDOoJHKlo2yBQqrmEGQBlKzBQnWQt1yirxU1nIuBas5NOIgO9z4pl2uJ4yj7tOK2KUh0U9RM8mV4rKUdUJf_4Ne-ikMabpEsaoQUnCWqMWGMsHHGLDV6-DS9WbNqL6LQ9_FoXdxJMGrre3U9Gh3-P39E_BmC0A00LUBBuPiX65I6xd1lTi14X67Duf_fKsvzs-WD4fIN1oXR7zdaVPouqxEJfWPzyeaHsvzY_H1pz4VfwC0sbR9</recordid><startdate>20110601</startdate><enddate>20110601</enddate><creator>Son, Jino</creator><creator>Lee, Sung-Eun</creator><creator>Park, Byeoung-Soo</creator><creator>Jung, Jinho</creator><creator>Park, Hyung Soon</creator><creator>Bang, Joo Young</creator><creator>Kang, Gum-Yong</creator><creator>Cho, Kijong</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley-VCH</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7U7</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>20110601</creationdate><title>Biomarker discovery and proteomic evaluation of cadmium toxicity on a collembolan species, Paronychiurus kimi (Lee)</title><author>Son, Jino ; Lee, Sung-Eun ; Park, Byeoung-Soo ; Jung, Jinho ; Park, Hyung Soon ; Bang, Joo Young ; Kang, Gum-Yong ; Cho, Kijong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5390-cbd33e23223a21ab59d6b9d4e3d91cea5caab5fa5adda8f20e269dd2253182ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>1-D PAGE</topic><topic>2-D PAGE</topic><topic>Analysis of Variance</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animal proteomics</topic><topic>Animals</topic><topic>Arthropods - drug effects</topic><topic>Arthropods - physiology</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>Biomarkers - analysis</topic><topic>Biomarkers - chemistry</topic><topic>Biosynthesis</topic><topic>Cadmium</topic><topic>Cadmium - toxicity</topic><topic>Chromatography, Liquid</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fundamental and applied biological sciences. 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Through proteomic analysis of P. kimi using 1‐D PAGE and nano‐LC‐MS/MS, 36 downregulated proteins and 40 upregulated proteins were found. Some of the downregulated and upregulated proteins were verified by LC‐MS/MS analysis after 2‐D PAGE. Downregulated proteins in response to cadmium exposure were involved in glycolysis and energy metabolism, chaperones, transcription, reproduction, and neuron growth. In contrast, proteins involved in glycolysis and energy production, neurogenesis, defense systems response to bacteria, and protein biosynthesis were upregulated in cadmium‐treated collembolans. Cubulin may be a potential biomarker for the detection of cadmium in P. kimi since this biomarker was able to low levels (3.5 mg/kg) of cadmium. The 14‐3‐3 ζ was also found to be a potential biomarker for the detection of medium levels (14 mg/kg) of cadmium. Collembolans may be an alternative tool to humans because many collembolans proteins show a high homology to human proteins.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>21548089</pmid><doi>10.1002/pmic.200900690</doi><tpages>14</tpages></addata></record> |
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| subjects | 1-D PAGE 2-D PAGE Analysis of Variance Analytical, structural and metabolic biochemistry Animal proteomics Animals Arthropods - drug effects Arthropods - physiology Biological and medical sciences Biomarkers Biomarkers - analysis Biomarkers - chemistry Biosynthesis Cadmium Cadmium - toxicity Chromatography, Liquid Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Insect Proteins - analysis Insect Proteins - chemistry Insect Proteins - classification Insect Proteins - metabolism LC-MS/MS Miscellaneous Proteins Proteome - chemistry Proteome - drug effects Proteomics - methods Receptors, Cell Surface Tandem Mass Spectrometry Toxicity |
| title | Biomarker discovery and proteomic evaluation of cadmium toxicity on a collembolan species, Paronychiurus kimi (Lee) |
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