Protein Coronas on Gold Nanorods Passivated with Amphiphilic Ligands Affect Cytotoxicity and Cellular Response to Penicillin/Streptomycin

We probe how amphiphilic ligands (ALs) of four different types affect the formation of protein coronas on gold nanorods (NRs) and their impact on cellular response. NRs coated with cetyltrimethylammonium bromide were ligand exchanged with polyoxyethylene[10]cetyl ether, oligofectamine, and phosphati...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:ACS nano 2014-05, Vol.8 (5), p.4608-4620
Hauptverfasser: Kah, James Chen Yong, Grabinski, Christin, Untener, Emily, Garrett, Carol, Chen, John, Zhu, David, Hussain, Saber M, Hamad-Schifferli, Kimberly
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 4620
container_issue 5
container_start_page 4608
container_title ACS nano
container_volume 8
creator Kah, James Chen Yong
Grabinski, Christin
Untener, Emily
Garrett, Carol
Chen, John
Zhu, David
Hussain, Saber M
Hamad-Schifferli, Kimberly
description We probe how amphiphilic ligands (ALs) of four different types affect the formation of protein coronas on gold nanorods (NRs) and their impact on cellular response. NRs coated with cetyltrimethylammonium bromide were ligand exchanged with polyoxyethylene[10]cetyl ether, oligofectamine, and phosphatidylserine (PS). Protein coronas from equine serum (ES) were formed on these NR-ALs, and their colloidal stability, as well as cell uptake, proliferation, oxidative stress, and gene expression, were examined. We find that the protein corona that forms and its colloidal stability are affected by AL type and that the cellular response to these NR-AL-coronas (NR-AL-ES) is both ligand and corona dependent. We also find that the presence of common cell culture supplement penicillin/streptomycin can impact the colloidal stability and cellular response of NR-AL and NR-AL-ES, showing that the cell response is not necessarily inert to pen/strep when in the presence of nanoparticles. Although the protein corona is what the cells see, the underlying surface ligands evidently play an important role in shaping and defining the physical characteristics of the corona, which ultimately impacts the cellular response. Further, the results of this study suggest that the cellular behavior toward NR-AL is mediated by not only the type of AL and the protein corona it forms but also its resulting colloidal stability and interaction with cell culture supplements.
doi_str_mv 10.1021/nn5002886
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1529842013</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1529842013</sourcerecordid><originalsourceid>FETCH-LOGICAL-a315t-e4fb9bfe3611510be9d700b37e5fcf8edf1459aae19e81e6d680057bf7c67b653</originalsourceid><addsrcrecordid>eNptkMFq3DAQhkVoyG6THvICRZdCetisZFuyfFxMkhaWZEla6M3I8ijRYkuuJLfdR8hbR2HTPQUGZpj5-Jn_R-ickktKMrq0lhGSCcGP0JxWOV8QwX99OMyMztDHELaEsFKU_ATNsqJkoqjYHD1vvItgLK6dd1YG7Cy-cX2Hb6VNmy7gjQzB_JEROvzXxCe8GsYnk6o3Cq_No7SJWWkNKuJ6F110_4wycYfTAdfQ91MvPb6HMDobAEeHN2AT0ffGLh-ihzG6YaeMPUPHWvYBPr31U_Tz-upH_W2xvrv5Xq_WC5lTFhdQ6LZqNeScUkZJC1VXEtLmJTCttIBO04JVUgKtQFDgHRevvltdKl62nOWn6GKvO3r3e4IQm8EElR6VFtwUGsqyShQZoXlCv-5R5V0IHnQzejNIv2soaV6Tbw7JJ_bzm-zUDtAdyP9RJ-DLHpAqNFs3eZtcviP0AqstjRA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1529842013</pqid></control><display><type>article</type><title>Protein Coronas on Gold Nanorods Passivated with Amphiphilic Ligands Affect Cytotoxicity and Cellular Response to Penicillin/Streptomycin</title><source>MEDLINE</source><source>ACS Publications</source><creator>Kah, James Chen Yong ; Grabinski, Christin ; Untener, Emily ; Garrett, Carol ; Chen, John ; Zhu, David ; Hussain, Saber M ; Hamad-Schifferli, Kimberly</creator><creatorcontrib>Kah, James Chen Yong ; Grabinski, Christin ; Untener, Emily ; Garrett, Carol ; Chen, John ; Zhu, David ; Hussain, Saber M ; Hamad-Schifferli, Kimberly</creatorcontrib><description>We probe how amphiphilic ligands (ALs) of four different types affect the formation of protein coronas on gold nanorods (NRs) and their impact on cellular response. NRs coated with cetyltrimethylammonium bromide were ligand exchanged with polyoxyethylene[10]cetyl ether, oligofectamine, and phosphatidylserine (PS). Protein coronas from equine serum (ES) were formed on these NR-ALs, and their colloidal stability, as well as cell uptake, proliferation, oxidative stress, and gene expression, were examined. We find that the protein corona that forms and its colloidal stability are affected by AL type and that the cellular response to these NR-AL-coronas (NR-AL-ES) is both ligand and corona dependent. We also find that the presence of common cell culture supplement penicillin/streptomycin can impact the colloidal stability and cellular response of NR-AL and NR-AL-ES, showing that the cell response is not necessarily inert to pen/strep when in the presence of nanoparticles. Although the protein corona is what the cells see, the underlying surface ligands evidently play an important role in shaping and defining the physical characteristics of the corona, which ultimately impacts the cellular response. Further, the results of this study suggest that the cellular behavior toward NR-AL is mediated by not only the type of AL and the protein corona it forms but also its resulting colloidal stability and interaction with cell culture supplements.</description><identifier>ISSN: 1936-0851</identifier><identifier>EISSN: 1936-086X</identifier><identifier>DOI: 10.1021/nn5002886</identifier><identifier>PMID: 24758495</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Adsorption ; Animals ; Blood Proteins - chemistry ; Cell Line ; Cell Proliferation ; Cetrimonium Compounds - chemistry ; Colloids - chemistry ; Endotoxins - chemistry ; Gene Expression Regulation ; Gold - chemistry ; Horses ; Humans ; Keratinocytes - cytology ; Ligands ; Metal Nanoparticles - chemistry ; Nanotechnology - methods ; Nanotubes - chemistry ; Oxidative Stress ; Penicillins - chemistry ; Streptomycin - chemistry</subject><ispartof>ACS nano, 2014-05, Vol.8 (5), p.4608-4620</ispartof><rights>Copyright © 2014 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a315t-e4fb9bfe3611510be9d700b37e5fcf8edf1459aae19e81e6d680057bf7c67b653</citedby><cites>FETCH-LOGICAL-a315t-e4fb9bfe3611510be9d700b37e5fcf8edf1459aae19e81e6d680057bf7c67b653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/nn5002886$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/nn5002886$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,782,786,2767,27083,27931,27932,56745,56795</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24758495$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kah, James Chen Yong</creatorcontrib><creatorcontrib>Grabinski, Christin</creatorcontrib><creatorcontrib>Untener, Emily</creatorcontrib><creatorcontrib>Garrett, Carol</creatorcontrib><creatorcontrib>Chen, John</creatorcontrib><creatorcontrib>Zhu, David</creatorcontrib><creatorcontrib>Hussain, Saber M</creatorcontrib><creatorcontrib>Hamad-Schifferli, Kimberly</creatorcontrib><title>Protein Coronas on Gold Nanorods Passivated with Amphiphilic Ligands Affect Cytotoxicity and Cellular Response to Penicillin/Streptomycin</title><title>ACS nano</title><addtitle>ACS Nano</addtitle><description>We probe how amphiphilic ligands (ALs) of four different types affect the formation of protein coronas on gold nanorods (NRs) and their impact on cellular response. NRs coated with cetyltrimethylammonium bromide were ligand exchanged with polyoxyethylene[10]cetyl ether, oligofectamine, and phosphatidylserine (PS). Protein coronas from equine serum (ES) were formed on these NR-ALs, and their colloidal stability, as well as cell uptake, proliferation, oxidative stress, and gene expression, were examined. We find that the protein corona that forms and its colloidal stability are affected by AL type and that the cellular response to these NR-AL-coronas (NR-AL-ES) is both ligand and corona dependent. We also find that the presence of common cell culture supplement penicillin/streptomycin can impact the colloidal stability and cellular response of NR-AL and NR-AL-ES, showing that the cell response is not necessarily inert to pen/strep when in the presence of nanoparticles. Although the protein corona is what the cells see, the underlying surface ligands evidently play an important role in shaping and defining the physical characteristics of the corona, which ultimately impacts the cellular response. Further, the results of this study suggest that the cellular behavior toward NR-AL is mediated by not only the type of AL and the protein corona it forms but also its resulting colloidal stability and interaction with cell culture supplements.</description><subject>Adsorption</subject><subject>Animals</subject><subject>Blood Proteins - chemistry</subject><subject>Cell Line</subject><subject>Cell Proliferation</subject><subject>Cetrimonium Compounds - chemistry</subject><subject>Colloids - chemistry</subject><subject>Endotoxins - chemistry</subject><subject>Gene Expression Regulation</subject><subject>Gold - chemistry</subject><subject>Horses</subject><subject>Humans</subject><subject>Keratinocytes - cytology</subject><subject>Ligands</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Nanotechnology - methods</subject><subject>Nanotubes - chemistry</subject><subject>Oxidative Stress</subject><subject>Penicillins - chemistry</subject><subject>Streptomycin - chemistry</subject><issn>1936-0851</issn><issn>1936-086X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkMFq3DAQhkVoyG6THvICRZdCetisZFuyfFxMkhaWZEla6M3I8ijRYkuuJLfdR8hbR2HTPQUGZpj5-Jn_R-ickktKMrq0lhGSCcGP0JxWOV8QwX99OMyMztDHELaEsFKU_ATNsqJkoqjYHD1vvItgLK6dd1YG7Cy-cX2Hb6VNmy7gjQzB_JEROvzXxCe8GsYnk6o3Cq_No7SJWWkNKuJ6F110_4wycYfTAdfQ91MvPb6HMDobAEeHN2AT0ffGLh-ihzG6YaeMPUPHWvYBPr31U_Tz-upH_W2xvrv5Xq_WC5lTFhdQ6LZqNeScUkZJC1VXEtLmJTCttIBO04JVUgKtQFDgHRevvltdKl62nOWn6GKvO3r3e4IQm8EElR6VFtwUGsqyShQZoXlCv-5R5V0IHnQzejNIv2soaV6Tbw7JJ_bzm-zUDtAdyP9RJ-DLHpAqNFs3eZtcviP0AqstjRA</recordid><startdate>20140527</startdate><enddate>20140527</enddate><creator>Kah, James Chen Yong</creator><creator>Grabinski, Christin</creator><creator>Untener, Emily</creator><creator>Garrett, Carol</creator><creator>Chen, John</creator><creator>Zhu, David</creator><creator>Hussain, Saber M</creator><creator>Hamad-Schifferli, Kimberly</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140527</creationdate><title>Protein Coronas on Gold Nanorods Passivated with Amphiphilic Ligands Affect Cytotoxicity and Cellular Response to Penicillin/Streptomycin</title><author>Kah, James Chen Yong ; Grabinski, Christin ; Untener, Emily ; Garrett, Carol ; Chen, John ; Zhu, David ; Hussain, Saber M ; Hamad-Schifferli, Kimberly</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a315t-e4fb9bfe3611510be9d700b37e5fcf8edf1459aae19e81e6d680057bf7c67b653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adsorption</topic><topic>Animals</topic><topic>Blood Proteins - chemistry</topic><topic>Cell Line</topic><topic>Cell Proliferation</topic><topic>Cetrimonium Compounds - chemistry</topic><topic>Colloids - chemistry</topic><topic>Endotoxins - chemistry</topic><topic>Gene Expression Regulation</topic><topic>Gold - chemistry</topic><topic>Horses</topic><topic>Humans</topic><topic>Keratinocytes - cytology</topic><topic>Ligands</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Nanotechnology - methods</topic><topic>Nanotubes - chemistry</topic><topic>Oxidative Stress</topic><topic>Penicillins - chemistry</topic><topic>Streptomycin - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kah, James Chen Yong</creatorcontrib><creatorcontrib>Grabinski, Christin</creatorcontrib><creatorcontrib>Untener, Emily</creatorcontrib><creatorcontrib>Garrett, Carol</creatorcontrib><creatorcontrib>Chen, John</creatorcontrib><creatorcontrib>Zhu, David</creatorcontrib><creatorcontrib>Hussain, Saber M</creatorcontrib><creatorcontrib>Hamad-Schifferli, Kimberly</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>ACS nano</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kah, James Chen Yong</au><au>Grabinski, Christin</au><au>Untener, Emily</au><au>Garrett, Carol</au><au>Chen, John</au><au>Zhu, David</au><au>Hussain, Saber M</au><au>Hamad-Schifferli, Kimberly</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein Coronas on Gold Nanorods Passivated with Amphiphilic Ligands Affect Cytotoxicity and Cellular Response to Penicillin/Streptomycin</atitle><jtitle>ACS nano</jtitle><addtitle>ACS Nano</addtitle><date>2014-05-27</date><risdate>2014</risdate><volume>8</volume><issue>5</issue><spage>4608</spage><epage>4620</epage><pages>4608-4620</pages><issn>1936-0851</issn><eissn>1936-086X</eissn><abstract>We probe how amphiphilic ligands (ALs) of four different types affect the formation of protein coronas on gold nanorods (NRs) and their impact on cellular response. NRs coated with cetyltrimethylammonium bromide were ligand exchanged with polyoxyethylene[10]cetyl ether, oligofectamine, and phosphatidylserine (PS). Protein coronas from equine serum (ES) were formed on these NR-ALs, and their colloidal stability, as well as cell uptake, proliferation, oxidative stress, and gene expression, were examined. We find that the protein corona that forms and its colloidal stability are affected by AL type and that the cellular response to these NR-AL-coronas (NR-AL-ES) is both ligand and corona dependent. We also find that the presence of common cell culture supplement penicillin/streptomycin can impact the colloidal stability and cellular response of NR-AL and NR-AL-ES, showing that the cell response is not necessarily inert to pen/strep when in the presence of nanoparticles. Although the protein corona is what the cells see, the underlying surface ligands evidently play an important role in shaping and defining the physical characteristics of the corona, which ultimately impacts the cellular response. Further, the results of this study suggest that the cellular behavior toward NR-AL is mediated by not only the type of AL and the protein corona it forms but also its resulting colloidal stability and interaction with cell culture supplements.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>24758495</pmid><doi>10.1021/nn5002886</doi><tpages>13</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1936-0851
ispartof ACS nano, 2014-05, Vol.8 (5), p.4608-4620
issn 1936-0851
1936-086X
language eng
recordid cdi_proquest_miscellaneous_1529842013
source MEDLINE; ACS Publications
subjects Adsorption
Animals
Blood Proteins - chemistry
Cell Line
Cell Proliferation
Cetrimonium Compounds - chemistry
Colloids - chemistry
Endotoxins - chemistry
Gene Expression Regulation
Gold - chemistry
Horses
Humans
Keratinocytes - cytology
Ligands
Metal Nanoparticles - chemistry
Nanotechnology - methods
Nanotubes - chemistry
Oxidative Stress
Penicillins - chemistry
Streptomycin - chemistry
title Protein Coronas on Gold Nanorods Passivated with Amphiphilic Ligands Affect Cytotoxicity and Cellular Response to Penicillin/Streptomycin
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-05T22%3A45%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Protein%20Coronas%20on%20Gold%20Nanorods%20Passivated%20with%20Amphiphilic%20Ligands%20Affect%20Cytotoxicity%20and%20Cellular%20Response%20to%20Penicillin/Streptomycin&rft.jtitle=ACS%20nano&rft.au=Kah,%20James%20Chen%20Yong&rft.date=2014-05-27&rft.volume=8&rft.issue=5&rft.spage=4608&rft.epage=4620&rft.pages=4608-4620&rft.issn=1936-0851&rft.eissn=1936-086X&rft_id=info:doi/10.1021/nn5002886&rft_dat=%3Cproquest_cross%3E1529842013%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1529842013&rft_id=info:pmid/24758495&rfr_iscdi=true