Increased Amounts of the Aspergillus Metabolite d-Mannitol in Tissue and Serum of Rats with Experimental Aspergillosis
Several Aspergillus species produce large amounts of the hexitol d-rnannitol in vitro, but it is not known whether these species also produce d-rnannitol in vivo. Serum samples and homogenized tissues were analyzed from rats pretreated with cortisone and cyclophosphamide and then given 2 × 106 prein...
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| Veröffentlicht in: | The Journal of infectious diseases 1989-07, Vol.160 (1), p.95-103 |
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| creator | Wong, Brian Brauer, Karen L. Tsai, Ring R. Jayasimhulu, Koka |
| description | Several Aspergillus species produce large amounts of the hexitol d-rnannitol in vitro, but it is not known whether these species also produce d-rnannitol in vivo. Serum samples and homogenized tissues were analyzed from rats pretreated with cortisone and cyclophosphamide and then given 2 × 106 preincubated conidia of Aspergillus jumigatus intravenously. The resulting infection was lethal by 48 h and was characterized by much more severe disease in the liver than in the kidneys, spleen, or lungs. A compound present in increased amounts in the livers and sera of the infected rats was shown to be d-mannitol by gas chromatography (GC) and GC/mass spectrometry and enzymatically. Quantitative analysis by GC showed that the infected rats had more d-mannitol in their livers (but not in their lungs or kidneys) after 12 h (P < .01 at 12, 24, and 36 h) and higher serum d-mannitol concentrations and serum d-mannitol/creatinine ratios after 36 h (P < .05) than did uninfected controls. These results indicate that A. jumigatus can produce and release sufficient d-mannitol in the tissues of infected animals to raise serum d-mannitol levels. Thus, d-mannitol is a potential diagnostic marker for aspergillosis. |
| doi_str_mv | 10.1093/infdis/160.1.95 |
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Serum samples and homogenized tissues were analyzed from rats pretreated with cortisone and cyclophosphamide and then given 2 × 106 preincubated conidia of Aspergillus jumigatus intravenously. The resulting infection was lethal by 48 h and was characterized by much more severe disease in the liver than in the kidneys, spleen, or lungs. A compound present in increased amounts in the livers and sera of the infected rats was shown to be d-mannitol by gas chromatography (GC) and GC/mass spectrometry and enzymatically. Quantitative analysis by GC showed that the infected rats had more d-mannitol in their livers (but not in their lungs or kidneys) after 12 h (P < .01 at 12, 24, and 36 h) and higher serum d-mannitol concentrations and serum d-mannitol/creatinine ratios after 36 h (P < .05) than did uninfected controls. These results indicate that A. jumigatus can produce and release sufficient d-mannitol in the tissues of infected animals to raise serum d-mannitol levels. Thus, d-mannitol is a potential diagnostic marker for aspergillosis.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/160.1.95</identifier><identifier>PMID: 2499640</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University Chicago Press</publisher><subject>Animals ; Aspergillosis ; Aspergillosis - diagnosis ; Aspergillosis - metabolism ; Aspergillus ; Aspergillus fumigatus - metabolism ; Biological and medical sciences ; Chromatography, Gas ; Conidia ; Creatinine - blood ; Gas Chromatography-Mass Spectrometry ; Homogenization ; Human mycoses ; Infections ; Infectious diseases ; Kidney - microbiology ; Kidneys ; Liver ; Liver - analysis ; Liver - microbiology ; Liver Diseases - diagnosis ; Liver Diseases - metabolism ; Lung - microbiology ; Major Articles ; Male ; Mannitol - analysis ; Mannitol - blood ; Medical sciences ; Metabolic diseases ; Mycoses ; Mycoses of the respiratory system ; Rats ; Rats, Inbred Strains ; Spleen - microbiology ; Tissue samples</subject><ispartof>The Journal of infectious diseases, 1989-07, Vol.160 (1), p.95-103</ispartof><rights>Copyright 1989 The University of Chicago</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c313t-156c314a87bc22acea4015efd181f1d7735b00222b956f42786cb9f51f56c2373</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30119685$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30119685$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27903,27904,57995,58228</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6848810$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2499640$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wong, Brian</creatorcontrib><creatorcontrib>Brauer, Karen L.</creatorcontrib><creatorcontrib>Tsai, Ring R.</creatorcontrib><creatorcontrib>Jayasimhulu, Koka</creatorcontrib><title>Increased Amounts of the Aspergillus Metabolite d-Mannitol in Tissue and Serum of Rats with Experimental Aspergillosis</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>Several Aspergillus species produce large amounts of the hexitol d-rnannitol in vitro, but it is not known whether these species also produce d-rnannitol in vivo. Serum samples and homogenized tissues were analyzed from rats pretreated with cortisone and cyclophosphamide and then given 2 × 106 preincubated conidia of Aspergillus jumigatus intravenously. The resulting infection was lethal by 48 h and was characterized by much more severe disease in the liver than in the kidneys, spleen, or lungs. A compound present in increased amounts in the livers and sera of the infected rats was shown to be d-mannitol by gas chromatography (GC) and GC/mass spectrometry and enzymatically. Quantitative analysis by GC showed that the infected rats had more d-mannitol in their livers (but not in their lungs or kidneys) after 12 h (P < .01 at 12, 24, and 36 h) and higher serum d-mannitol concentrations and serum d-mannitol/creatinine ratios after 36 h (P < .05) than did uninfected controls. These results indicate that A. jumigatus can produce and release sufficient d-mannitol in the tissues of infected animals to raise serum d-mannitol levels. Thus, d-mannitol is a potential diagnostic marker for aspergillosis.</description><subject>Animals</subject><subject>Aspergillosis</subject><subject>Aspergillosis - diagnosis</subject><subject>Aspergillosis - metabolism</subject><subject>Aspergillus</subject><subject>Aspergillus fumigatus - metabolism</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Gas</subject><subject>Conidia</subject><subject>Creatinine - blood</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Homogenization</subject><subject>Human mycoses</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Kidney - microbiology</subject><subject>Kidneys</subject><subject>Liver</subject><subject>Liver - analysis</subject><subject>Liver - microbiology</subject><subject>Liver Diseases - diagnosis</subject><subject>Liver Diseases - metabolism</subject><subject>Lung - microbiology</subject><subject>Major Articles</subject><subject>Male</subject><subject>Mannitol - analysis</subject><subject>Mannitol - blood</subject><subject>Medical sciences</subject><subject>Metabolic diseases</subject><subject>Mycoses</subject><subject>Mycoses of the respiratory system</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Spleen - microbiology</subject><subject>Tissue samples</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtv1DAUhS0EKtPCmhWSF4hdOn7Er-WoKrRiKl6lQmwsJ7GpS-IMuU4p_x6PZpiufK1zzmffg9ArSk4pMXwZU-giLKks91MjnqAFFVxVUlL-FC0IYayi2pjn6BjgjhBSc6mO0BGrjZE1WaD7y9RO3oHv8GoY55QBjwHnW49XsPHTz9j3M-Arn10z9jF73FVXLqWYxx7HhK8jwOyxSx3-6qd52Ia_uAL5E_MtPn8oiDj4lF3_yBshwgv0LLge_Mv9eYK-vTu_Pruo1h_fX56t1lXLKc8VFbIMtdOqaRlzrXc1ocKHjmoaaKcUF812R9YYIUPNlJZtY4KgoQQZV_wEvd1xN9P4e_aQ7RCh9X3vkh9nsFQwQ5SmxbjcGdtpBJh8sJvyczf9tZTYbdN217QtTVtqjSiJ13v03Ay-O_j31Rb9zV530Lo-TC61Jf_fJnWtNSWPmDvI43SQOaHUSL19ptrpEbJ_OOhu-mWl4krYi-8_LFvf6E8fPt9Yxf8BBJGhYg</recordid><startdate>19890701</startdate><enddate>19890701</enddate><creator>Wong, Brian</creator><creator>Brauer, Karen L.</creator><creator>Tsai, Ring R.</creator><creator>Jayasimhulu, Koka</creator><general>The University Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope></search><sort><creationdate>19890701</creationdate><title>Increased Amounts of the Aspergillus Metabolite d-Mannitol in Tissue and Serum of Rats with Experimental Aspergillosis</title><author>Wong, Brian ; Brauer, Karen L. ; Tsai, Ring R. ; Jayasimhulu, Koka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c313t-156c314a87bc22acea4015efd181f1d7735b00222b956f42786cb9f51f56c2373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Aspergillosis</topic><topic>Aspergillosis - diagnosis</topic><topic>Aspergillosis - metabolism</topic><topic>Aspergillus</topic><topic>Aspergillus fumigatus - metabolism</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Gas</topic><topic>Conidia</topic><topic>Creatinine - blood</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Homogenization</topic><topic>Human mycoses</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Kidney - microbiology</topic><topic>Kidneys</topic><topic>Liver</topic><topic>Liver - analysis</topic><topic>Liver - microbiology</topic><topic>Liver Diseases - diagnosis</topic><topic>Liver Diseases - metabolism</topic><topic>Lung - microbiology</topic><topic>Major Articles</topic><topic>Male</topic><topic>Mannitol - analysis</topic><topic>Mannitol - blood</topic><topic>Medical sciences</topic><topic>Metabolic diseases</topic><topic>Mycoses</topic><topic>Mycoses of the respiratory system</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Spleen - microbiology</topic><topic>Tissue samples</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wong, Brian</creatorcontrib><creatorcontrib>Brauer, Karen L.</creatorcontrib><creatorcontrib>Tsai, Ring R.</creatorcontrib><creatorcontrib>Jayasimhulu, Koka</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wong, Brian</au><au>Brauer, Karen L.</au><au>Tsai, Ring R.</au><au>Jayasimhulu, Koka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased Amounts of the Aspergillus Metabolite d-Mannitol in Tissue and Serum of Rats with Experimental Aspergillosis</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1989-07-01</date><risdate>1989</risdate><volume>160</volume><issue>1</issue><spage>95</spage><epage>103</epage><pages>95-103</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Several Aspergillus species produce large amounts of the hexitol d-rnannitol in vitro, but it is not known whether these species also produce d-rnannitol in vivo. Serum samples and homogenized tissues were analyzed from rats pretreated with cortisone and cyclophosphamide and then given 2 × 106 preincubated conidia of Aspergillus jumigatus intravenously. The resulting infection was lethal by 48 h and was characterized by much more severe disease in the liver than in the kidneys, spleen, or lungs. A compound present in increased amounts in the livers and sera of the infected rats was shown to be d-mannitol by gas chromatography (GC) and GC/mass spectrometry and enzymatically. Quantitative analysis by GC showed that the infected rats had more d-mannitol in their livers (but not in their lungs or kidneys) after 12 h (P < .01 at 12, 24, and 36 h) and higher serum d-mannitol concentrations and serum d-mannitol/creatinine ratios after 36 h (P < .05) than did uninfected controls. These results indicate that A. jumigatus can produce and release sufficient d-mannitol in the tissues of infected animals to raise serum d-mannitol levels. Thus, d-mannitol is a potential diagnostic marker for aspergillosis.</abstract><cop>Chicago, IL</cop><pub>The University Chicago Press</pub><pmid>2499640</pmid><doi>10.1093/infdis/160.1.95</doi><tpages>9</tpages></addata></record> |
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| subjects | Animals Aspergillosis Aspergillosis - diagnosis Aspergillosis - metabolism Aspergillus Aspergillus fumigatus - metabolism Biological and medical sciences Chromatography, Gas Conidia Creatinine - blood Gas Chromatography-Mass Spectrometry Homogenization Human mycoses Infections Infectious diseases Kidney - microbiology Kidneys Liver Liver - analysis Liver - microbiology Liver Diseases - diagnosis Liver Diseases - metabolism Lung - microbiology Major Articles Male Mannitol - analysis Mannitol - blood Medical sciences Metabolic diseases Mycoses Mycoses of the respiratory system Rats Rats, Inbred Strains Spleen - microbiology Tissue samples |
| title | Increased Amounts of the Aspergillus Metabolite d-Mannitol in Tissue and Serum of Rats with Experimental Aspergillosis |
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