Evaluation of a Brix refractometer to estimate serum immunoglobulin G concentration in neonatal dairy calves
The objective of this study was to evaluate the utility of a digital Brix refractometer for the assessment of success of passive transfer of maternal immunoglobulin compared with the measurement of serum total protein (STP) by refractometry. Blood samples (n=400) were collected from calves at 3 to 6...
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| Veröffentlicht in: | Journal of dairy science 2014-06, Vol.97 (6), p.3838-3844 |
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| creator | Deelen, S.M. Ollivett, T.L. Haines, D.M. Leslie, K.E. |
| description | The objective of this study was to evaluate the utility of a digital Brix refractometer for the assessment of success of passive transfer of maternal immunoglobulin compared with the measurement of serum total protein (STP) by refractometry. Blood samples (n=400) were collected from calves at 3 to 6d of age. Serum IgG concentration was determined by radial immunodiffusion (RID), and STP and percentage Brix (%Brix) were determined using a digital refractometer. The mean IgG concentration was 24.1g/L [standard deviation (SD) ± 10.0] with a range from 2.1 to 59.1g/L. The mean STP concentration was 6.0g/dL (SD ± 0.8) with a range from 4.4 to 8.8g/dL. The mean %Brix concentration was 9.2% (SD ± 0.9) with a range of 7.3 to 12.4%. Brix percentage was highly correlated with IgG (r=0.93). Test characteristics were calculated to assess failure of passive transfer (FPT; serum IgG |
| doi_str_mv | 10.3168/jds.2014-7939 |
| format | Article |
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Blood samples (n=400) were collected from calves at 3 to 6d of age. Serum IgG concentration was determined by radial immunodiffusion (RID), and STP and percentage Brix (%Brix) were determined using a digital refractometer. The mean IgG concentration was 24.1g/L [standard deviation (SD) ± 10.0] with a range from 2.1 to 59.1g/L. The mean STP concentration was 6.0g/dL (SD ± 0.8) with a range from 4.4 to 8.8g/dL. The mean %Brix concentration was 9.2% (SD ± 0.9) with a range of 7.3 to 12.4%. Brix percentage was highly correlated with IgG (r=0.93). Test characteristics were calculated to assess failure of passive transfer (FPT; serum IgG <10g/L). The sensitivity and specificity of STP at 5.5g/dL were 76.3 and 94.4%, respectively. A receiver operating characteristic curve was created to plot the true positive rate against the false positive rate for consecutive %Brix values. The optimal combination of sensitivity (88.9%) and specificity (88.9%) was at 8.4% Brix. Serum total protein was also positively correlated with %Brix (r=1.00) and IgG (r=0.93). Dairy producers can successfully monitor their colostrum management and the overall success of passive transfer using a digital Brix refractometer to estimate IgG concentration of colostrum and calf serum.</description><identifier>ISSN: 0022-0302</identifier><identifier>EISSN: 1525-3198</identifier><identifier>DOI: 10.3168/jds.2014-7939</identifier><identifier>PMID: 24704239</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Animals, Newborn ; calf ; Cattle ; Colostrum - chemistry ; Immunodiffusion - methods ; Immunodiffusion - veterinary ; immunoglobulin ; Immunoglobulin G - blood ; passive transfer ; refractometer ; Refractometry - instrumentation ; Refractometry - veterinary ; Sensitivity and Specificity</subject><ispartof>Journal of dairy science, 2014-06, Vol.97 (6), p.3838-3844</ispartof><rights>2014 American Dairy Science Association</rights><rights>Copyright © 2014 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-a46fa7781ffd96789add94ed18483eba2e494e2ee924922a59ade36032a8f7953</citedby><cites>FETCH-LOGICAL-c380t-a46fa7781ffd96789add94ed18483eba2e494e2ee924922a59ade36032a8f7953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.3168/jds.2014-7939$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24704239$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Deelen, S.M.</creatorcontrib><creatorcontrib>Ollivett, T.L.</creatorcontrib><creatorcontrib>Haines, D.M.</creatorcontrib><creatorcontrib>Leslie, K.E.</creatorcontrib><title>Evaluation of a Brix refractometer to estimate serum immunoglobulin G concentration in neonatal dairy calves</title><title>Journal of dairy science</title><addtitle>J Dairy Sci</addtitle><description>The objective of this study was to evaluate the utility of a digital Brix refractometer for the assessment of success of passive transfer of maternal immunoglobulin compared with the measurement of serum total protein (STP) by refractometry. Blood samples (n=400) were collected from calves at 3 to 6d of age. Serum IgG concentration was determined by radial immunodiffusion (RID), and STP and percentage Brix (%Brix) were determined using a digital refractometer. The mean IgG concentration was 24.1g/L [standard deviation (SD) ± 10.0] with a range from 2.1 to 59.1g/L. The mean STP concentration was 6.0g/dL (SD ± 0.8) with a range from 4.4 to 8.8g/dL. The mean %Brix concentration was 9.2% (SD ± 0.9) with a range of 7.3 to 12.4%. Brix percentage was highly correlated with IgG (r=0.93). Test characteristics were calculated to assess failure of passive transfer (FPT; serum IgG <10g/L). The sensitivity and specificity of STP at 5.5g/dL were 76.3 and 94.4%, respectively. A receiver operating characteristic curve was created to plot the true positive rate against the false positive rate for consecutive %Brix values. The optimal combination of sensitivity (88.9%) and specificity (88.9%) was at 8.4% Brix. Serum total protein was also positively correlated with %Brix (r=1.00) and IgG (r=0.93). Dairy producers can successfully monitor their colostrum management and the overall success of passive transfer using a digital Brix refractometer to estimate IgG concentration of colostrum and calf serum.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>calf</subject><subject>Cattle</subject><subject>Colostrum - chemistry</subject><subject>Immunodiffusion - methods</subject><subject>Immunodiffusion - veterinary</subject><subject>immunoglobulin</subject><subject>Immunoglobulin G - blood</subject><subject>passive transfer</subject><subject>refractometer</subject><subject>Refractometry - instrumentation</subject><subject>Refractometry - veterinary</subject><subject>Sensitivity and Specificity</subject><issn>0022-0302</issn><issn>1525-3198</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtrGzEURkVpqJ3HstuiZTeT6jEPadkaJykEsknW4lq6UxQ0I1fSmPrfR8Zpd10IcS-Hj_sdQj5zdit5r769unwrGG-bQUv9gax5J7pGcq0-kjVjQjRMMrEilzm_1pEL1n0iK9EOrBVSr0nYHiAsUHycaRwp0B_J_6EJxwS2xAkLJloixVz8BAVpxrRM1E_TMsdfIe6W4Gd6T22cLc4lnYPqasY4Q4FAHfh0pBbCAfM1uRghZLx5_6_Iy932efPQPD7d_9x8f2ysVKw00PYjDIPi4-h0PygNzukWHVetkrgDgW0dBaIWrRYCugqg7JkUoMZBd_KKfD3n7lP8vdTbzeSzxRCgnrVkUx0pKfv6KtqcUZtizrW32afaNB0NZ-Yk2FTB5iTYnARX_st79LKb0P2j_xqtwHAGsBY8eEwmW4_VjvMJbTEu-v9EvwHd7Ysh</recordid><startdate>20140601</startdate><enddate>20140601</enddate><creator>Deelen, S.M.</creator><creator>Ollivett, T.L.</creator><creator>Haines, D.M.</creator><creator>Leslie, K.E.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140601</creationdate><title>Evaluation of a Brix refractometer to estimate serum immunoglobulin G concentration in neonatal dairy calves</title><author>Deelen, S.M. ; Ollivett, T.L. ; Haines, D.M. ; Leslie, K.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-a46fa7781ffd96789add94ed18483eba2e494e2ee924922a59ade36032a8f7953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>calf</topic><topic>Cattle</topic><topic>Colostrum - chemistry</topic><topic>Immunodiffusion - methods</topic><topic>Immunodiffusion - veterinary</topic><topic>immunoglobulin</topic><topic>Immunoglobulin G - blood</topic><topic>passive transfer</topic><topic>refractometer</topic><topic>Refractometry - instrumentation</topic><topic>Refractometry - veterinary</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deelen, S.M.</creatorcontrib><creatorcontrib>Ollivett, T.L.</creatorcontrib><creatorcontrib>Haines, D.M.</creatorcontrib><creatorcontrib>Leslie, K.E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dairy science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deelen, S.M.</au><au>Ollivett, T.L.</au><au>Haines, D.M.</au><au>Leslie, K.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of a Brix refractometer to estimate serum immunoglobulin G concentration in neonatal dairy calves</atitle><jtitle>Journal of dairy science</jtitle><addtitle>J Dairy Sci</addtitle><date>2014-06-01</date><risdate>2014</risdate><volume>97</volume><issue>6</issue><spage>3838</spage><epage>3844</epage><pages>3838-3844</pages><issn>0022-0302</issn><eissn>1525-3198</eissn><abstract>The objective of this study was to evaluate the utility of a digital Brix refractometer for the assessment of success of passive transfer of maternal immunoglobulin compared with the measurement of serum total protein (STP) by refractometry. Blood samples (n=400) were collected from calves at 3 to 6d of age. Serum IgG concentration was determined by radial immunodiffusion (RID), and STP and percentage Brix (%Brix) were determined using a digital refractometer. The mean IgG concentration was 24.1g/L [standard deviation (SD) ± 10.0] with a range from 2.1 to 59.1g/L. The mean STP concentration was 6.0g/dL (SD ± 0.8) with a range from 4.4 to 8.8g/dL. The mean %Brix concentration was 9.2% (SD ± 0.9) with a range of 7.3 to 12.4%. Brix percentage was highly correlated with IgG (r=0.93). Test characteristics were calculated to assess failure of passive transfer (FPT; serum IgG <10g/L). The sensitivity and specificity of STP at 5.5g/dL were 76.3 and 94.4%, respectively. A receiver operating characteristic curve was created to plot the true positive rate against the false positive rate for consecutive %Brix values. The optimal combination of sensitivity (88.9%) and specificity (88.9%) was at 8.4% Brix. Serum total protein was also positively correlated with %Brix (r=1.00) and IgG (r=0.93). Dairy producers can successfully monitor their colostrum management and the overall success of passive transfer using a digital Brix refractometer to estimate IgG concentration of colostrum and calf serum.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>24704239</pmid><doi>10.3168/jds.2014-7939</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Animals, Newborn calf Cattle Colostrum - chemistry Immunodiffusion - methods Immunodiffusion - veterinary immunoglobulin Immunoglobulin G - blood passive transfer refractometer Refractometry - instrumentation Refractometry - veterinary Sensitivity and Specificity |
| title | Evaluation of a Brix refractometer to estimate serum immunoglobulin G concentration in neonatal dairy calves |
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