Nitrogen‐limited behaviour of micro‐organisms growing in the presence of large concentrations of ammonium ions
Cells of Klebsiella pneumoniae NCTC 418 grown at low culture pH values (4.5–5) in a glucose‐limited chemostat culture contained elevated levels of glutamate synthase (EC 2.6.1.53). This can be taken as an indication that these cells show the physiology of nitrogen‐limited cells, in spite of the fact...
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| Veröffentlicht in: | FEMS microbiology letters 1989-04, Vol.58 (2‐3), p.229-232 |
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| container_issue | 2‐3 |
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| container_title | FEMS microbiology letters |
| container_volume | 58 |
| creator | Buurman, Ed T. Mattos, M.Joost Teixeira Neijssel, Oense M. |
| description | Cells of Klebsiella pneumoniae NCTC 418 grown at low culture pH values (4.5–5) in a glucose‐limited chemostat culture contained elevated levels of glutamate synthase (EC 2.6.1.53). This can be taken as an indication that these cells show the physiology of nitrogen‐limited cells, in spite of the fact that high concentrations (about 80 mM) of ammonium ions were present in the culture extracellular fluids. This phenomenon can be explained by the rapid diffusion of ammonia (NH3) through the cell membrane, leading to very low cytoplasmic ammonium (NH4+) and NH3 levels in cells that possess an almost neutral cytoplasmic pH value, but are growing at low culture pH values. |
| doi_str_mv | 10.1111/j.1574-6968.1989.tb03049.x |
| format | Article |
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This can be taken as an indication that these cells show the physiology of nitrogen‐limited cells, in spite of the fact that high concentrations (about 80 mM) of ammonium ions were present in the culture extracellular fluids. This phenomenon can be explained by the rapid diffusion of ammonia (NH3) through the cell membrane, leading to very low cytoplasmic ammonium (NH4+) and NH3 levels in cells that possess an almost neutral cytoplasmic pH value, but are growing at low culture pH values.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1989.tb03049.x</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>ammonia ; Ammonia assimilation ; Bacteriology ; Biological and medical sciences ; Chemostat culture ; Fundamental and applied biological sciences. 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This can be taken as an indication that these cells show the physiology of nitrogen‐limited cells, in spite of the fact that high concentrations (about 80 mM) of ammonium ions were present in the culture extracellular fluids. This phenomenon can be explained by the rapid diffusion of ammonia (NH3) through the cell membrane, leading to very low cytoplasmic ammonium (NH4+) and NH3 levels in cells that possess an almost neutral cytoplasmic pH value, but are growing at low culture pH values.</description><subject>ammonia</subject><subject>Ammonia assimilation</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Chemostat culture</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutamate dehydrogenase</subject><subject>Glutamate synthase</subject><subject>Klebsiella pneumoniae</subject><subject>Metabolism. 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Psychology</topic><topic>Glutamate dehydrogenase</topic><topic>Glutamate synthase</topic><topic>Klebsiella pneumoniae</topic><topic>Metabolism. Enzymes</topic><topic>Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Buurman, Ed T.</creatorcontrib><creatorcontrib>Mattos, M.Joost Teixeira</creatorcontrib><creatorcontrib>Neijssel, Oense M.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Buurman, Ed T.</au><au>Mattos, M.Joost Teixeira</au><au>Neijssel, Oense M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitrogen‐limited behaviour of micro‐organisms growing in the presence of large concentrations of ammonium ions</atitle><jtitle>FEMS microbiology letters</jtitle><date>1989-04</date><risdate>1989</risdate><volume>58</volume><issue>2‐3</issue><spage>229</spage><epage>232</epage><pages>229-232</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Cells of Klebsiella pneumoniae NCTC 418 grown at low culture pH values (4.5–5) in a glucose‐limited chemostat culture contained elevated levels of glutamate synthase (EC 2.6.1.53). 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| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1989-04, Vol.58 (2‐3), p.229-232 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15282299 |
| source | Oxford University Press Journals Digital Archive Legacy; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | ammonia Ammonia assimilation Bacteriology Biological and medical sciences Chemostat culture Fundamental and applied biological sciences. Psychology Glutamate dehydrogenase Glutamate synthase Klebsiella pneumoniae Metabolism. Enzymes Microbiology |
| title | Nitrogen‐limited behaviour of micro‐organisms growing in the presence of large concentrations of ammonium ions |
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