Evaluation of an enzyme-linked immunosorbent assay in the serodiagnosis of amoebic liver abscess

An indirect enzyme-linked immunosorbent assay (ELISA) was evaluated for the detection of anti-amoebic IGG and IGM antibodies to assess its value in distinguishing past from current infection in invasive amoebiasis, particularly in amoebic liver abscess (ALA) patients. Using sera from 295 individuals...

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Veröffentlicht in:	South African Medical Journal/Suid-Afrikaanse Mediese Tydskrift 1988-01, Vol.74 (12), p.625-628
Hauptverfasser:	Sathar, MA, Simjee, A E, Delarey Nel, J, Bredenkamp, BLF, Gathiram, V, Jackson, TFHG
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Sprache:	eng
Schlagworte:	abscesses amebiasis enzyme-linked immunosorbent assay liver man
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creator	Sathar, MA Simjee, A E Delarey Nel, J Bredenkamp, BLF Gathiram, V Jackson, TFHG
description	An indirect enzyme-linked immunosorbent assay (ELISA) was evaluated for the detection of anti-amoebic IGG and IGM antibodies to assess its value in distinguishing past from current infection in invasive amoebiasis, particularly in amoebic liver abscess (ALA) patients. Using sera from 295 individuals, the ELISA was also compared with the amoebic gel diffusion (AGD) test. In 100 patients the IgG-ELISA at a single test dilution of 1/6,400 had a sensitivity of 99% for clinically diagnosed ALA. In these same patients the IGM-ELISA at a single dilution of 1/400, had a sensitivity of 64% and a specificity of 97.9%.
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issn	0038-2469
language	eng
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source	Alma/SFX Local Collection
subjects	abscesses amebiasis enzyme-linked immunosorbent assay liver man
title	Evaluation of an enzyme-linked immunosorbent assay in the serodiagnosis of amoebic liver abscess
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