Proliferation kinetics and cell lineages can be studied in whole mounts and macerates by means of BrdU/anti-BrdU technique
S-phase cells in intact animals of the coelenterate species Eirene viridula, Hydractinia echinata, Hydra attenuata, and Hydra magnipapillata incorporate the thymidine analogue bromodeoxyuridine (BrdU) into newly synthesized DNA. BrdU-labelled nuclei divide and cells appear to undergo normal differen...
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| Veröffentlicht in: | Development (Cambridge) 1988-08, Vol.103 (4), p.791-794 |
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| container_title | Development (Cambridge) |
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| creator | Plickert, G Kroiher, M |
| description | S-phase cells in intact animals of the coelenterate species Eirene viridula, Hydractinia echinata, Hydra attenuata, and Hydra magnipapillata incorporate the thymidine analogue bromodeoxyuridine (BrdU) into newly synthesized DNA. BrdU-labelled nuclei divide and cells appear to undergo normal differentiation. Whole-mount preparations and macerated tissues were screened for S-phase cells by means of immunocytochemical detection of BrdU (Gratzner, 1982). It is demonstrated that spatial patterns of DNA replication can be evaluated easily. Cell lineages and pathways of cell migration could be traced. |
| doi_str_mv | 10.1242/dev.103.4.791 |
| format | Article |
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BrdU-labelled nuclei divide and cells appear to undergo normal differentiation. Whole-mount preparations and macerated tissues were screened for S-phase cells by means of immunocytochemical detection of BrdU (Gratzner, 1982). It is demonstrated that spatial patterns of DNA replication can be evaluated easily. Cell lineages and pathways of cell migration could be traced.</description><identifier>ISSN: 0950-1991</identifier><identifier>EISSN: 1477-9129</identifier><identifier>DOI: 10.1242/dev.103.4.791</identifier><identifier>PMID: 3073936</identifier><language>eng</language><publisher>England: The Company of Biologists Limited</publisher><subject>Animals ; Bromodeoxyuridine ; Cell Differentiation ; Cell Division ; DNA Replication ; Eirene viridula ; Freshwater ; Hydra attenuata ; Hydra magnipapillata ; Hydractinia echinata ; Immunohistochemistry - methods ; Marine ; Microscopy, Fluorescence ; Microscopy, Phase-Contrast</subject><ispartof>Development (Cambridge), 1988-08, Vol.103 (4), p.791-794</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-138b2861f8c5e2236635292ce859f01f9b720a1b41bd0a784032c9cdcd333c803</citedby><cites>FETCH-LOGICAL-c356t-138b2861f8c5e2236635292ce859f01f9b720a1b41bd0a784032c9cdcd333c803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3665,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3073936$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Plickert, G</creatorcontrib><creatorcontrib>Kroiher, M</creatorcontrib><title>Proliferation kinetics and cell lineages can be studied in whole mounts and macerates by means of BrdU/anti-BrdU technique</title><title>Development (Cambridge)</title><addtitle>Development</addtitle><description>S-phase cells in intact animals of the coelenterate species Eirene viridula, Hydractinia echinata, Hydra attenuata, and Hydra magnipapillata incorporate the thymidine analogue bromodeoxyuridine (BrdU) into newly synthesized DNA. BrdU-labelled nuclei divide and cells appear to undergo normal differentiation. Whole-mount preparations and macerated tissues were screened for S-phase cells by means of immunocytochemical detection of BrdU (Gratzner, 1982). It is demonstrated that spatial patterns of DNA replication can be evaluated easily. Cell lineages and pathways of cell migration could be traced.</description><subject>Animals</subject><subject>Bromodeoxyuridine</subject><subject>Cell Differentiation</subject><subject>Cell Division</subject><subject>DNA Replication</subject><subject>Eirene viridula</subject><subject>Freshwater</subject><subject>Hydra attenuata</subject><subject>Hydra magnipapillata</subject><subject>Hydractinia echinata</subject><subject>Immunohistochemistry - methods</subject><subject>Marine</subject><subject>Microscopy, Fluorescence</subject><subject>Microscopy, Phase-Contrast</subject><issn>0950-1991</issn><issn>1477-9129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkD1vFDEQhq0IFC4hZUokV1TsxWPvl0uIgCBFgoLUlteevTPs2oftTRR-fby6E6LyyPPMM5qXkGtgW-A1v7H4uAUmtvW2k3BGNlB3XSWBy1dkw2TDKpAS3pCLlH4xxkTbdefkXLBOSNFuyN8fMUxuxKizC57-dh6zM4lqb6nBaaJT-dE7TNRoTwekKS_WoaXO06d9mJDOYfH5ODBrs4oKPDzTGbVPNIz0U7QPN9pnV60VzWj23v1Z8C15Peop4dXpvSQPXz7_vL2r7r9__Xb78b4yomlzBaIfeN_C2JsGORdtKxouucG-kSODUQ4dZxqGGgbLdNfXTHAjjTVWCGF6Ji7J-6P3EENZm7KaXVpv0x7DkhQ0vIe6gQJWR9DEkFLEUR2im3V8VsDUmrUqWZdaqFqVrAv_7iRehhntP_oUbul_OPb3brd_chHV4MIUdi7ltKpwCof_dC-RpoqT</recordid><startdate>19880801</startdate><enddate>19880801</enddate><creator>Plickert, G</creator><creator>Kroiher, M</creator><general>The Company of Biologists Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19880801</creationdate><title>Proliferation kinetics and cell lineages can be studied in whole mounts and macerates by means of BrdU/anti-BrdU technique</title><author>Plickert, G ; Kroiher, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-138b2861f8c5e2236635292ce859f01f9b720a1b41bd0a784032c9cdcd333c803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Bromodeoxyuridine</topic><topic>Cell Differentiation</topic><topic>Cell Division</topic><topic>DNA Replication</topic><topic>Eirene viridula</topic><topic>Freshwater</topic><topic>Hydra attenuata</topic><topic>Hydra magnipapillata</topic><topic>Hydractinia echinata</topic><topic>Immunohistochemistry - methods</topic><topic>Marine</topic><topic>Microscopy, Fluorescence</topic><topic>Microscopy, Phase-Contrast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Plickert, G</creatorcontrib><creatorcontrib>Kroiher, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Development (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Plickert, G</au><au>Kroiher, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proliferation kinetics and cell lineages can be studied in whole mounts and macerates by means of BrdU/anti-BrdU technique</atitle><jtitle>Development (Cambridge)</jtitle><addtitle>Development</addtitle><date>1988-08-01</date><risdate>1988</risdate><volume>103</volume><issue>4</issue><spage>791</spage><epage>794</epage><pages>791-794</pages><issn>0950-1991</issn><eissn>1477-9129</eissn><abstract>S-phase cells in intact animals of the coelenterate species Eirene viridula, Hydractinia echinata, Hydra attenuata, and Hydra magnipapillata incorporate the thymidine analogue bromodeoxyuridine (BrdU) into newly synthesized DNA. 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| fulltext | fulltext |
| identifier | ISSN: 0950-1991 |
| ispartof | Development (Cambridge), 1988-08, Vol.103 (4), p.791-794 |
| issn | 0950-1991 1477-9129 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Company of Biologists |
| subjects | Animals Bromodeoxyuridine Cell Differentiation Cell Division DNA Replication Eirene viridula Freshwater Hydra attenuata Hydra magnipapillata Hydractinia echinata Immunohistochemistry - methods Marine Microscopy, Fluorescence Microscopy, Phase-Contrast |
| title | Proliferation kinetics and cell lineages can be studied in whole mounts and macerates by means of BrdU/anti-BrdU technique |
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