Measurements of elastic modulus for human anterior lens capsule with atomic force microscopy: the effect of loading force
The purpose of the study was to appraise the effect of loading force magnitude on the determination of the elastic modulus of the anterior lens capsule through atomic force microscopy. Four human anterior lens capsules taken during phacoemulsification cataract surgery were studied, free of epithelia...
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| Veröffentlicht in: | International ophthalmology 2014-06, Vol.34 (3), p.519-523 |
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| creator | Tsaousis, Konstantinos T. Karagiannidis, Panagiotis G. Kopsachilis, Nikolaos Symeonidis, Chrysanthos Tsinopoulos, Ioannis T. Karagkiozaki, Varvara Lamprogiannis, Lampros P. Logothetidis, Stergios |
| description | The purpose of the study was to appraise the effect of loading force magnitude on the determination of the elastic modulus of the anterior lens capsule through atomic force microscopy. Four human anterior lens capsules taken during phacoemulsification cataract surgery were studied, free of epithelial cells, with atomic force microscopy. For the experiment, five different indentation loading forces were applied to near areas of the specimen. Experimental data was exported and analyzed according to the Hertz model to obtain the Young’s modulus with regards to the elastic behavior of the material. Force–distance curves were acquired by applying a load of 2, 5, 10, 20 and 30 nN. When examining the results it was evident that determination of Young’s modulus of the anterior lens capsule is dependent on the loading force concerning the examined range. Loading forces of 10 and 20 nN led to results without significant difference (
p
> 0.05) and more reproducible (coefficients of variation 12.4 and 11.7 %, respectively). |
| doi_str_mv | 10.1007/s10792-013-9846-z |
| format | Article |
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p
> 0.05) and more reproducible (coefficients of variation 12.4 and 11.7 %, respectively).</description><identifier>ISSN: 0165-5701</identifier><identifier>EISSN: 1573-2630</identifier><identifier>DOI: 10.1007/s10792-013-9846-z</identifier><identifier>PMID: 24037592</identifier><identifier>CODEN: INOPDR</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Aged ; Aged, 80 and over ; Cataract - physiopathology ; Elastic Modulus ; Elastic Tissue ; Elasticity - physiology ; Humans ; Lens Capsule, Crystalline - physiology ; Medicine ; Medicine & Public Health ; Microscopy, Atomic Force ; Ophthalmology ; Original Paper ; Reproducibility of Results ; Stress, Mechanical</subject><ispartof>International ophthalmology, 2014-06, Vol.34 (3), p.519-523</ispartof><rights>Springer Science+Business Media Dordrecht 2013</rights><rights>Springer Science+Business Media Dordrecht 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-c4e7d8aa201dce87c45c351123621458ffc400d8cff6d43964d8b87bbca2e8543</citedby><cites>FETCH-LOGICAL-c442t-c4e7d8aa201dce87c45c351123621458ffc400d8cff6d43964d8b87bbca2e8543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10792-013-9846-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10792-013-9846-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24037592$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsaousis, Konstantinos T.</creatorcontrib><creatorcontrib>Karagiannidis, Panagiotis G.</creatorcontrib><creatorcontrib>Kopsachilis, Nikolaos</creatorcontrib><creatorcontrib>Symeonidis, Chrysanthos</creatorcontrib><creatorcontrib>Tsinopoulos, Ioannis T.</creatorcontrib><creatorcontrib>Karagkiozaki, Varvara</creatorcontrib><creatorcontrib>Lamprogiannis, Lampros P.</creatorcontrib><creatorcontrib>Logothetidis, Stergios</creatorcontrib><title>Measurements of elastic modulus for human anterior lens capsule with atomic force microscopy: the effect of loading force</title><title>International ophthalmology</title><addtitle>Int Ophthalmol</addtitle><addtitle>Int Ophthalmol</addtitle><description>The purpose of the study was to appraise the effect of loading force magnitude on the determination of the elastic modulus of the anterior lens capsule through atomic force microscopy. Four human anterior lens capsules taken during phacoemulsification cataract surgery were studied, free of epithelial cells, with atomic force microscopy. For the experiment, five different indentation loading forces were applied to near areas of the specimen. Experimental data was exported and analyzed according to the Hertz model to obtain the Young’s modulus with regards to the elastic behavior of the material. Force–distance curves were acquired by applying a load of 2, 5, 10, 20 and 30 nN. When examining the results it was evident that determination of Young’s modulus of the anterior lens capsule is dependent on the loading force concerning the examined range. Loading forces of 10 and 20 nN led to results without significant difference (
p
> 0.05) and more reproducible (coefficients of variation 12.4 and 11.7 %, respectively).</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Cataract - physiopathology</subject><subject>Elastic Modulus</subject><subject>Elastic Tissue</subject><subject>Elasticity - physiology</subject><subject>Humans</subject><subject>Lens Capsule, Crystalline - physiology</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Microscopy, Atomic Force</subject><subject>Ophthalmology</subject><subject>Original Paper</subject><subject>Reproducibility of Results</subject><subject>Stress, Mechanical</subject><issn>0165-5701</issn><issn>1573-2630</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1kUtv1DAUhS1URIcpP4ANstQNm4CfsdNdVQGtVMSGri2Pc91JlcSDH0LTX4-jFFRVYuOH7nfO9fVB6D0lnygh6nOiRHWsIZQ3nRZt8_gKbahUvGEtJydoQ2grG6kIPUVvU3oghHSqa9-gUyYIV7JjG3T8DjaVCBPMOeHgMYw25cHhKfRlLAn7EPG-THbGds4Qh3odYU7Y2UMqI-DfQ95jm8NUNZV1gOsphuTC4XiB8x4weA8uL95jsP0w36_cGXrt7Zjg3dO-RXdfv_y8um5uf3y7ubq8bZwQLNcVVK-tZYT2DrRyQjouKWW8ZVRI7b0ThPTaed_2gnet6PVOq93OWQZaCr5FH1ffQwy_CqRspiE5GEc7QyjJUMkU51TVP9ui8xfoQyhxrq9bqJYSqllXKbpSy5gpgjeHOEw2Hg0lZsnFrLmYmotZcjGPVfPhybnsJuj_Kf4GUQG2AqmW5nuIz1r_1_UPq2aaSw</recordid><startdate>20140601</startdate><enddate>20140601</enddate><creator>Tsaousis, Konstantinos T.</creator><creator>Karagiannidis, Panagiotis G.</creator><creator>Kopsachilis, Nikolaos</creator><creator>Symeonidis, Chrysanthos</creator><creator>Tsinopoulos, Ioannis T.</creator><creator>Karagkiozaki, Varvara</creator><creator>Lamprogiannis, Lampros P.</creator><creator>Logothetidis, Stergios</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20140601</creationdate><title>Measurements of elastic modulus for human anterior lens capsule with atomic force microscopy: the effect of loading force</title><author>Tsaousis, Konstantinos T. ; Karagiannidis, Panagiotis G. ; Kopsachilis, Nikolaos ; Symeonidis, Chrysanthos ; Tsinopoulos, Ioannis T. ; Karagkiozaki, Varvara ; Lamprogiannis, Lampros P. ; Logothetidis, Stergios</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-c4e7d8aa201dce87c45c351123621458ffc400d8cff6d43964d8b87bbca2e8543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Cataract - physiopathology</topic><topic>Elastic Modulus</topic><topic>Elastic Tissue</topic><topic>Elasticity - physiology</topic><topic>Humans</topic><topic>Lens Capsule, Crystalline - physiology</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Microscopy, Atomic Force</topic><topic>Ophthalmology</topic><topic>Original Paper</topic><topic>Reproducibility of Results</topic><topic>Stress, Mechanical</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsaousis, Konstantinos T.</creatorcontrib><creatorcontrib>Karagiannidis, Panagiotis G.</creatorcontrib><creatorcontrib>Kopsachilis, Nikolaos</creatorcontrib><creatorcontrib>Symeonidis, Chrysanthos</creatorcontrib><creatorcontrib>Tsinopoulos, Ioannis T.</creatorcontrib><creatorcontrib>Karagkiozaki, Varvara</creatorcontrib><creatorcontrib>Lamprogiannis, Lampros P.</creatorcontrib><creatorcontrib>Logothetidis, Stergios</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>International ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsaousis, Konstantinos T.</au><au>Karagiannidis, Panagiotis G.</au><au>Kopsachilis, Nikolaos</au><au>Symeonidis, Chrysanthos</au><au>Tsinopoulos, Ioannis T.</au><au>Karagkiozaki, Varvara</au><au>Lamprogiannis, Lampros P.</au><au>Logothetidis, Stergios</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measurements of elastic modulus for human anterior lens capsule with atomic force microscopy: the effect of loading force</atitle><jtitle>International ophthalmology</jtitle><stitle>Int Ophthalmol</stitle><addtitle>Int Ophthalmol</addtitle><date>2014-06-01</date><risdate>2014</risdate><volume>34</volume><issue>3</issue><spage>519</spage><epage>523</epage><pages>519-523</pages><issn>0165-5701</issn><eissn>1573-2630</eissn><coden>INOPDR</coden><abstract>The purpose of the study was to appraise the effect of loading force magnitude on the determination of the elastic modulus of the anterior lens capsule through atomic force microscopy. Four human anterior lens capsules taken during phacoemulsification cataract surgery were studied, free of epithelial cells, with atomic force microscopy. For the experiment, five different indentation loading forces were applied to near areas of the specimen. Experimental data was exported and analyzed according to the Hertz model to obtain the Young’s modulus with regards to the elastic behavior of the material. Force–distance curves were acquired by applying a load of 2, 5, 10, 20 and 30 nN. When examining the results it was evident that determination of Young’s modulus of the anterior lens capsule is dependent on the loading force concerning the examined range. Loading forces of 10 and 20 nN led to results without significant difference (
p
> 0.05) and more reproducible (coefficients of variation 12.4 and 11.7 %, respectively).</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>24037592</pmid><doi>10.1007/s10792-013-9846-z</doi><tpages>5</tpages></addata></record> |
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| ispartof | International ophthalmology, 2014-06, Vol.34 (3), p.519-523 |
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| subjects | Aged Aged, 80 and over Cataract - physiopathology Elastic Modulus Elastic Tissue Elasticity - physiology Humans Lens Capsule, Crystalline - physiology Medicine Medicine & Public Health Microscopy, Atomic Force Ophthalmology Original Paper Reproducibility of Results Stress, Mechanical |
| title | Measurements of elastic modulus for human anterior lens capsule with atomic force microscopy: the effect of loading force |
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