Measurement of Serum Interleukin-2 Activity
The accurate measurement of serum interleukin-2 activity is crucial for assessing the efficacy and toxicity of systemic immunotherapy with recombinant interleukin-2. Incubation of serum at 56 °C for 30 minutes facilitates the bioassay for interleukin-2 activity by destroying the interleukin-2 Inhibi...
Gespeichert in:
| Veröffentlicht in: | Immunological investigations 1989-01, Vol.18 (5), p.713-722 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 722 |
|---|---|
| container_issue | 5 |
| container_start_page | 713 |
| container_title | Immunological investigations |
| container_volume | 18 |
| creator | Fleischmann, J. D. Wentworth, D. B. Thomas, K. H. Imbembo, A. L. |
| description | The accurate measurement of serum interleukin-2 activity is crucial for assessing the efficacy and toxicity of systemic immunotherapy with recombinant interleukin-2. Incubation of serum at 56 °C for 30 minutes facilitates the bioassay for interleukin-2 activity by destroying the interleukin-2 Inhibitory activity native to human serum. As this report will demonstrate, however, 30% to 50% of interleukin-2 activity in serum taken from patients or normal volunteers was destroyed by heating at 56°C. No loss of recombinant interleukin-2 activity occured during heating in serum-free media. The percentage of interleukin-2 activity lost at 56°C varied from patient to patient and also varied with the time of exposure. Native serum interleukin-2 inhibitory activity can be removed, and interleukin-2 activity can be assessed accurately in serial dilutions of the serum beyond 1:64. |
| doi_str_mv | 10.3109/08820138909057757 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_15262459</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>15262459</sourcerecordid><originalsourceid>FETCH-LOGICAL-c462t-7c1129d6ddc7431b406764bbba2c9d944cdf3d5a963587bf55be6f83502331143</originalsourceid><addsrcrecordid>eNp9kEtLAzEUhYMotVZ_gAuhG93IaN4zQTel-IKKC3UdMkmGTp1HTTJK_70pHRURurqL853DuQeAYwQvCILiEmYZhohkAgrI0pSlO2CIGMEJJQjtguFaTyIg9sGB9wsIIWFcDMAAc44oEkNw_miV75ytbRPGbTF-tq6rxw9NsK6y3VvZJHg80aH8KMPqEOwVqvL2qL8j8Hp78zK9T2ZPdw_TySzRlOOQpBohLAw3RqexR04hTznN81xhLYygVJuCGKYEJyxL84Kx3PIiIwxiEmtTMgJnm9yla98764OsS69tVanGtp2XiGGOKRMRRBtQu9Z7Zwu5dGWt3EoiKNcDyX8DRc9JH97ltTU_jn6RqJ_2uvJaVYVTjS79b7Aggmcpjtz1hiubonW1-mxdZWRQq6p13yayrcbVH_vcqirMtXJWLtrONXHfLU98AfrwkKE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15262459</pqid></control><display><type>article</type><title>Measurement of Serum Interleukin-2 Activity</title><source>MEDLINE</source><source>Taylor & Francis:Master (3349 titles)</source><source>Taylor & Francis Medical Library - CRKN</source><creator>Fleischmann, J. D. ; Wentworth, D. B. ; Thomas, K. H. ; Imbembo, A. L.</creator><creatorcontrib>Fleischmann, J. D. ; Wentworth, D. B. ; Thomas, K. H. ; Imbembo, A. L.</creatorcontrib><description>The accurate measurement of serum interleukin-2 activity is crucial for assessing the efficacy and toxicity of systemic immunotherapy with recombinant interleukin-2. Incubation of serum at 56 °C for 30 minutes facilitates the bioassay for interleukin-2 activity by destroying the interleukin-2 Inhibitory activity native to human serum. As this report will demonstrate, however, 30% to 50% of interleukin-2 activity in serum taken from patients or normal volunteers was destroyed by heating at 56°C. No loss of recombinant interleukin-2 activity occured during heating in serum-free media. The percentage of interleukin-2 activity lost at 56°C varied from patient to patient and also varied with the time of exposure. Native serum interleukin-2 inhibitory activity can be removed, and interleukin-2 activity can be assessed accurately in serial dilutions of the serum beyond 1:64.</description><identifier>ISSN: 0882-0139</identifier><identifier>EISSN: 1532-4311</identifier><identifier>DOI: 10.3109/08820138909057757</identifier><identifier>PMID: 2661419</identifier><identifier>CODEN: IMINEJ</identifier><language>eng</language><publisher>Monticello, NY: Informa UK Ltd</publisher><subject>Analysis of the immune response. Humoral and cellular immunity ; Biological and medical sciences ; Chromatography, High Pressure Liquid ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Hot Temperature ; Humans ; Immunobiology ; Indicator Dilution Techniques ; Interleukin-2 - blood ; Lymphokines, interleukins ( function, expression) ; Recombinant Proteins - analysis ; Regulatory factors and their cellular receptors ; Temperature ; Time Factors</subject><ispartof>Immunological investigations, 1989-01, Vol.18 (5), p.713-722</ispartof><rights>1989 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1989</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-7c1129d6ddc7431b406764bbba2c9d944cdf3d5a963587bf55be6f83502331143</citedby><cites>FETCH-LOGICAL-c462t-7c1129d6ddc7431b406764bbba2c9d944cdf3d5a963587bf55be6f83502331143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/08820138909057757$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/08820138909057757$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,59647,59753,60436,60542,61221,61256,61402,61437</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19396872$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2661419$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fleischmann, J. D.</creatorcontrib><creatorcontrib>Wentworth, D. B.</creatorcontrib><creatorcontrib>Thomas, K. H.</creatorcontrib><creatorcontrib>Imbembo, A. L.</creatorcontrib><title>Measurement of Serum Interleukin-2 Activity</title><title>Immunological investigations</title><addtitle>Immunol Invest</addtitle><description>The accurate measurement of serum interleukin-2 activity is crucial for assessing the efficacy and toxicity of systemic immunotherapy with recombinant interleukin-2. Incubation of serum at 56 °C for 30 minutes facilitates the bioassay for interleukin-2 activity by destroying the interleukin-2 Inhibitory activity native to human serum. As this report will demonstrate, however, 30% to 50% of interleukin-2 activity in serum taken from patients or normal volunteers was destroyed by heating at 56°C. No loss of recombinant interleukin-2 activity occured during heating in serum-free media. The percentage of interleukin-2 activity lost at 56°C varied from patient to patient and also varied with the time of exposure. Native serum interleukin-2 inhibitory activity can be removed, and interleukin-2 activity can be assessed accurately in serial dilutions of the serum beyond 1:64.</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>Indicator Dilution Techniques</subject><subject>Interleukin-2 - blood</subject><subject>Lymphokines, interleukins ( function, expression)</subject><subject>Recombinant Proteins - analysis</subject><subject>Regulatory factors and their cellular receptors</subject><subject>Temperature</subject><subject>Time Factors</subject><issn>0882-0139</issn><issn>1532-4311</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLAzEUhYMotVZ_gAuhG93IaN4zQTel-IKKC3UdMkmGTp1HTTJK_70pHRURurqL853DuQeAYwQvCILiEmYZhohkAgrI0pSlO2CIGMEJJQjtguFaTyIg9sGB9wsIIWFcDMAAc44oEkNw_miV75ytbRPGbTF-tq6rxw9NsK6y3VvZJHg80aH8KMPqEOwVqvL2qL8j8Hp78zK9T2ZPdw_TySzRlOOQpBohLAw3RqexR04hTznN81xhLYygVJuCGKYEJyxL84Kx3PIiIwxiEmtTMgJnm9yla98764OsS69tVanGtp2XiGGOKRMRRBtQu9Z7Zwu5dGWt3EoiKNcDyX8DRc9JH97ltTU_jn6RqJ_2uvJaVYVTjS79b7Aggmcpjtz1hiubonW1-mxdZWRQq6p13yayrcbVH_vcqirMtXJWLtrONXHfLU98AfrwkKE</recordid><startdate>19890101</startdate><enddate>19890101</enddate><creator>Fleischmann, J. D.</creator><creator>Wentworth, D. B.</creator><creator>Thomas, K. H.</creator><creator>Imbembo, A. L.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Dekker</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope></search><sort><creationdate>19890101</creationdate><title>Measurement of Serum Interleukin-2 Activity</title><author>Fleischmann, J. D. ; Wentworth, D. B. ; Thomas, K. H. ; Imbembo, A. L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-7c1129d6ddc7431b406764bbba2c9d944cdf3d5a963587bf55be6f83502331143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Hot Temperature</topic><topic>Humans</topic><topic>Immunobiology</topic><topic>Indicator Dilution Techniques</topic><topic>Interleukin-2 - blood</topic><topic>Lymphokines, interleukins ( function, expression)</topic><topic>Recombinant Proteins - analysis</topic><topic>Regulatory factors and their cellular receptors</topic><topic>Temperature</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fleischmann, J. D.</creatorcontrib><creatorcontrib>Wentworth, D. B.</creatorcontrib><creatorcontrib>Thomas, K. H.</creatorcontrib><creatorcontrib>Imbembo, A. L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Immunological investigations</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fleischmann, J. D.</au><au>Wentworth, D. B.</au><au>Thomas, K. H.</au><au>Imbembo, A. L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Measurement of Serum Interleukin-2 Activity</atitle><jtitle>Immunological investigations</jtitle><addtitle>Immunol Invest</addtitle><date>1989-01-01</date><risdate>1989</risdate><volume>18</volume><issue>5</issue><spage>713</spage><epage>722</epage><pages>713-722</pages><issn>0882-0139</issn><eissn>1532-4311</eissn><coden>IMINEJ</coden><abstract>The accurate measurement of serum interleukin-2 activity is crucial for assessing the efficacy and toxicity of systemic immunotherapy with recombinant interleukin-2. Incubation of serum at 56 °C for 30 minutes facilitates the bioassay for interleukin-2 activity by destroying the interleukin-2 Inhibitory activity native to human serum. As this report will demonstrate, however, 30% to 50% of interleukin-2 activity in serum taken from patients or normal volunteers was destroyed by heating at 56°C. No loss of recombinant interleukin-2 activity occured during heating in serum-free media. The percentage of interleukin-2 activity lost at 56°C varied from patient to patient and also varied with the time of exposure. Native serum interleukin-2 inhibitory activity can be removed, and interleukin-2 activity can be assessed accurately in serial dilutions of the serum beyond 1:64.</abstract><cop>Monticello, NY</cop><pub>Informa UK Ltd</pub><pmid>2661419</pmid><doi>10.3109/08820138909057757</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0882-0139 |
| ispartof | Immunological investigations, 1989-01, Vol.18 (5), p.713-722 |
| issn | 0882-0139 1532-4311 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15262459 |
| source | MEDLINE; Taylor & Francis:Master (3349 titles); Taylor & Francis Medical Library - CRKN |
| subjects | Analysis of the immune response. Humoral and cellular immunity Biological and medical sciences Chromatography, High Pressure Liquid Fundamental and applied biological sciences. Psychology Fundamental immunology Hot Temperature Humans Immunobiology Indicator Dilution Techniques Interleukin-2 - blood Lymphokines, interleukins ( function, expression) Recombinant Proteins - analysis Regulatory factors and their cellular receptors Temperature Time Factors |
| title | Measurement of Serum Interleukin-2 Activity |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T18%3A32%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Measurement%20of%20Serum%20Interleukin-2%20Activity&rft.jtitle=Immunological%20investigations&rft.au=Fleischmann,%20J.%20D.&rft.date=1989-01-01&rft.volume=18&rft.issue=5&rft.spage=713&rft.epage=722&rft.pages=713-722&rft.issn=0882-0139&rft.eissn=1532-4311&rft.coden=IMINEJ&rft_id=info:doi/10.3109/08820138909057757&rft_dat=%3Cproquest_cross%3E15262459%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15262459&rft_id=info:pmid/2661419&rfr_iscdi=true |