Detection of donor-specific-antibodies by solid phase assay and its relevance to complement-dependent-lymphocytotoxicity cross-matching in kidney transplantation
Abstract Presensitization against a broad array of HLA is associated with prolonged waiting times and inferior kidney allogaft survival. Although the use of solid phase assay (SPA) for the detection and characterization of anti-HLA antibodies provides greater sensitivity than complement-dependent ly...
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| Veröffentlicht in: | Human immunology 2014-06, Vol.75 (6), p.520-523 |
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| creator | Ho, Eric K Vasilescu, Elena R Vlad, George Clynes, Raphael A Ratner, Lloyd E Suciu-Foca, Nicole |
| description | Abstract Presensitization against a broad array of HLA is associated with prolonged waiting times and inferior kidney allogaft survival. Although the use of solid phase assay (SPA) for the detection and characterization of anti-HLA antibodies provides greater sensitivity than complement-dependent lymphocytotoxicity (CDC) assay, it often detects donor specific antibodies (DSA) which turn out to be clinically irrelevant. Our data reinforce the concept that these two types of assays should be used in parallel for pre-and post-transplantation monitoring of anti-HLA antibodies in recipients of solid organ allografts. |
| doi_str_mv | 10.1016/j.humimm.2014.03.002 |
| format | Article |
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Although the use of solid phase assay (SPA) for the detection and characterization of anti-HLA antibodies provides greater sensitivity than complement-dependent lymphocytotoxicity (CDC) assay, it often detects donor specific antibodies (DSA) which turn out to be clinically irrelevant. Our data reinforce the concept that these two types of assays should be used in parallel for pre-and post-transplantation monitoring of anti-HLA antibodies in recipients of solid organ allografts.</description><identifier>ISSN: 0198-8859</identifier><identifier>EISSN: 1879-1166</identifier><identifier>DOI: 10.1016/j.humimm.2014.03.002</identifier><identifier>PMID: 24662417</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Aged ; Allergy and Immunology ; Antilymphocyte Serum - blood ; B-Lymphocytes - cytology ; B-Lymphocytes - immunology ; Complement System Proteins - metabolism ; Complement-dependent lymphocytotoxicity ; Cross-matching ; Donor-specific antibodies ; Female ; Gene Expression ; Graft Rejection - prevention & control ; Graft Survival ; Histocompatibility Testing ; HLA Antigens - genetics ; HLA Antigens - immunology ; Humans ; Immunoassay - methods ; Isoantibodies - blood ; Kidney Transplantation ; Male ; Middle Aged ; Solid phase assay ; T-Lymphocytes - cytology ; T-Lymphocytes - immunology ; Tissue Donors ; Transplantation, Homologous</subject><ispartof>Human immunology, 2014-06, Vol.75 (6), p.520-523</ispartof><rights>American Society for Histocompatibility and Immunogenetics</rights><rights>2014 American Society for Histocompatibility and Immunogenetics</rights><rights>Copyright © 2014 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-f19cea64891cb5cd83d3b650e8f4328f5aca8e941c1194621115f648021cc9e73</citedby><cites>FETCH-LOGICAL-c417t-f19cea64891cb5cd83d3b650e8f4328f5aca8e941c1194621115f648021cc9e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0198885914001104$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24662417$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ho, Eric K</creatorcontrib><creatorcontrib>Vasilescu, Elena R</creatorcontrib><creatorcontrib>Vlad, George</creatorcontrib><creatorcontrib>Clynes, Raphael A</creatorcontrib><creatorcontrib>Ratner, Lloyd E</creatorcontrib><creatorcontrib>Suciu-Foca, Nicole</creatorcontrib><title>Detection of donor-specific-antibodies by solid phase assay and its relevance to complement-dependent-lymphocytotoxicity cross-matching in kidney transplantation</title><title>Human immunology</title><addtitle>Hum Immunol</addtitle><description>Abstract Presensitization against a broad array of HLA is associated with prolonged waiting times and inferior kidney allogaft survival. Although the use of solid phase assay (SPA) for the detection and characterization of anti-HLA antibodies provides greater sensitivity than complement-dependent lymphocytotoxicity (CDC) assay, it often detects donor specific antibodies (DSA) which turn out to be clinically irrelevant. Our data reinforce the concept that these two types of assays should be used in parallel for pre-and post-transplantation monitoring of anti-HLA antibodies in recipients of solid organ allografts.</description><subject>Adult</subject><subject>Aged</subject><subject>Allergy and Immunology</subject><subject>Antilymphocyte Serum - blood</subject><subject>B-Lymphocytes - cytology</subject><subject>B-Lymphocytes - immunology</subject><subject>Complement System Proteins - metabolism</subject><subject>Complement-dependent lymphocytotoxicity</subject><subject>Cross-matching</subject><subject>Donor-specific antibodies</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Graft Rejection - prevention & control</subject><subject>Graft Survival</subject><subject>Histocompatibility Testing</subject><subject>HLA Antigens - genetics</subject><subject>HLA Antigens - immunology</subject><subject>Humans</subject><subject>Immunoassay - methods</subject><subject>Isoantibodies - blood</subject><subject>Kidney Transplantation</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Solid phase assay</subject><subject>T-Lymphocytes - cytology</subject><subject>T-Lymphocytes - immunology</subject><subject>Tissue Donors</subject><subject>Transplantation, Homologous</subject><issn>0198-8859</issn><issn>1879-1166</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkr-O1DAQxiME4paDN0DIJU2CJ3GyToOE7vgnnUQB1JZjT9jZS-xge0_kcXhTHPagoKGyi2_mm29-UxTPgVfAoXt1rA6nmea5qjmIijcV5_WDYgdy35cAXfew2HHoZSll218UT2I8cs73fC8eFxe16LpawH5X_LzGhCaRd8yPzHrnQxkXNDSSKbVLNHhLGNmwsugnsmw56IhMx6hXpp1llCILOOGddgZZ8sz4eZlwRpdKiws6u_2mdV4O3qzJJ_-DDKWVmeBjLGedzIHcN0aO3ZJ1uLIUtIvLlM31NtfT4tGop4jP7t_L4uu7t1-uPpQ3n95_vHpzU5ocJJUj9AZ1J2QPZmiNlY1thq7lKEfR1HJstdESewEGoBddDQDtmOW8BmN63DeXxctz3yX47yeMSc0UDU55EPSnqKCtO6illJtUnKW_MwQc1RJo1mFVwNUGRx3VGY7a4CjeqAwnl724dzgNM9q_RX9oZMHrswBzzjvCoKIhzHu1FDIkZT39z-HfBmYiR0ZPt7hiPPpTcHmHClSsFVeftwPZ7gME5wBcNL8AGPW8AA</recordid><startdate>20140601</startdate><enddate>20140601</enddate><creator>Ho, Eric K</creator><creator>Vasilescu, Elena R</creator><creator>Vlad, George</creator><creator>Clynes, Raphael A</creator><creator>Ratner, Lloyd E</creator><creator>Suciu-Foca, Nicole</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20140601</creationdate><title>Detection of donor-specific-antibodies by solid phase assay and its relevance to complement-dependent-lymphocytotoxicity cross-matching in kidney transplantation</title><author>Ho, Eric K ; Vasilescu, Elena R ; Vlad, George ; Clynes, Raphael A ; Ratner, Lloyd E ; Suciu-Foca, Nicole</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-f19cea64891cb5cd83d3b650e8f4328f5aca8e941c1194621115f648021cc9e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Allergy and Immunology</topic><topic>Antilymphocyte Serum - blood</topic><topic>B-Lymphocytes - cytology</topic><topic>B-Lymphocytes - immunology</topic><topic>Complement System Proteins - metabolism</topic><topic>Complement-dependent lymphocytotoxicity</topic><topic>Cross-matching</topic><topic>Donor-specific antibodies</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Graft Rejection - prevention & control</topic><topic>Graft Survival</topic><topic>Histocompatibility Testing</topic><topic>HLA Antigens - genetics</topic><topic>HLA Antigens - immunology</topic><topic>Humans</topic><topic>Immunoassay - methods</topic><topic>Isoantibodies - blood</topic><topic>Kidney Transplantation</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Solid phase assay</topic><topic>T-Lymphocytes - cytology</topic><topic>T-Lymphocytes - immunology</topic><topic>Tissue Donors</topic><topic>Transplantation, Homologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ho, Eric K</creatorcontrib><creatorcontrib>Vasilescu, Elena R</creatorcontrib><creatorcontrib>Vlad, George</creatorcontrib><creatorcontrib>Clynes, Raphael A</creatorcontrib><creatorcontrib>Ratner, Lloyd E</creatorcontrib><creatorcontrib>Suciu-Foca, Nicole</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Human immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ho, Eric K</au><au>Vasilescu, Elena R</au><au>Vlad, George</au><au>Clynes, Raphael A</au><au>Ratner, Lloyd E</au><au>Suciu-Foca, Nicole</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of donor-specific-antibodies by solid phase assay and its relevance to complement-dependent-lymphocytotoxicity cross-matching in kidney transplantation</atitle><jtitle>Human immunology</jtitle><addtitle>Hum Immunol</addtitle><date>2014-06-01</date><risdate>2014</risdate><volume>75</volume><issue>6</issue><spage>520</spage><epage>523</epage><pages>520-523</pages><issn>0198-8859</issn><eissn>1879-1166</eissn><abstract>Abstract Presensitization against a broad array of HLA is associated with prolonged waiting times and inferior kidney allogaft survival. Although the use of solid phase assay (SPA) for the detection and characterization of anti-HLA antibodies provides greater sensitivity than complement-dependent lymphocytotoxicity (CDC) assay, it often detects donor specific antibodies (DSA) which turn out to be clinically irrelevant. Our data reinforce the concept that these two types of assays should be used in parallel for pre-and post-transplantation monitoring of anti-HLA antibodies in recipients of solid organ allografts.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>24662417</pmid><doi>10.1016/j.humimm.2014.03.002</doi><tpages>4</tpages></addata></record> |
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| ispartof | Human immunology, 2014-06, Vol.75 (6), p.520-523 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Aged Allergy and Immunology Antilymphocyte Serum - blood B-Lymphocytes - cytology B-Lymphocytes - immunology Complement System Proteins - metabolism Complement-dependent lymphocytotoxicity Cross-matching Donor-specific antibodies Female Gene Expression Graft Rejection - prevention & control Graft Survival Histocompatibility Testing HLA Antigens - genetics HLA Antigens - immunology Humans Immunoassay - methods Isoantibodies - blood Kidney Transplantation Male Middle Aged Solid phase assay T-Lymphocytes - cytology T-Lymphocytes - immunology Tissue Donors Transplantation, Homologous |
| title | Detection of donor-specific-antibodies by solid phase assay and its relevance to complement-dependent-lymphocytotoxicity cross-matching in kidney transplantation |
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