Differential regulation of calcitonin gene-related peptide and substance P in cultured neonatal rat vagal sensory neurons
Nodose (inferior vagal sensory) ganglia were removed from neonatal rats, enzymatically dispersed using neutral protease, and maintained on previously dispersed rat atriacytes. After 7–10 days in culture, calcitonin gene-related peptide (CGRP) was present in 1–3 times the molar amount of substance P...
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| Veröffentlicht in: | Brain research 1989-01, Vol.478 (2), p.349-355 |
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| creator | MacLean, David B. Bennett, Barbara Morris, Mariana Wheeler, Frances B. |
| description | Nodose (inferior vagal sensory) ganglia were removed from neonatal rats, enzymatically dispersed using neutral protease, and maintained on previously dispersed rat atriacytes. After 7–10 days in culture, calcitonin gene-related peptide (CGRP) was present in 1–3 times the molar amount of substance P (SP). The content of SP was doubled by the addition of nerve growth factor (NGF) whereas CGRP was significantly less increased by 50% or less. The addition of forskolin increased SP and CGRP levels in cultures with or without NGF by 60–80 percent. Phorbol ester (PMA) did not alter SP content but significantly raised CGRP content by 40% in NGF supplemented cultures (
P < 0.001). Corticosterone, reduced SP content by 30% independently of NGF but had no effect on CGRP. These studies demonstrate that SP in vagal sensory neurons is more sensitive than CGRP to the effects of NGF or corticosterone. Both peptides are up-regulated by presumed increases in intracellular cyclic AMP, while CGRP (or CGRP neurons) may be independently regulated by protein kinase C. |
| doi_str_mv | 10.1016/0006-8993(89)91515-1 |
| format | Article |
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P < 0.001). Corticosterone, reduced SP content by 30% independently of NGF but had no effect on CGRP. These studies demonstrate that SP in vagal sensory neurons is more sensitive than CGRP to the effects of NGF or corticosterone. Both peptides are up-regulated by presumed increases in intracellular cyclic AMP, while CGRP (or CGRP neurons) may be independently regulated by protein kinase C.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/0006-8993(89)91515-1</identifier><identifier>PMID: 2466532</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Biochemistry and metabolism ; Biological and medical sciences ; Calcitonin Gene-Related Peptide ; Cell Count ; Cells, Cultured ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; Colforsin - pharmacology ; Corticosterone - pharmacology ; Fundamental and applied biological sciences. Psychology ; Nerve growth factor ; Nerve Growth Factors - pharmacology ; Neurons, Afferent - drug effects ; Neurons, Afferent - metabolism ; Neuropeptides - metabolism ; Nodose ganglion ; Nodose Ganglion - cytology ; Nodose Ganglion - drug effects ; Nodose Ganglion - metabolism ; Phorbol Esters - pharmacology ; Rats ; Rats, Inbred Strains ; Substance P ; Substance P - metabolism ; Tissue culture ; Vagus nerve ; Vagus Nerve - cytology ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research, 1989-01, Vol.478 (2), p.349-355</ispartof><rights>1989</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c446t-591f424134763a90e772015b1c8a608f590168314fcdefafdcfca9dc68bef1e73</citedby><cites>FETCH-LOGICAL-c446t-591f424134763a90e772015b1c8a608f590168314fcdefafdcfca9dc68bef1e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006899389915151$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6763879$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6965465$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2466532$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MacLean, David B.</creatorcontrib><creatorcontrib>Bennett, Barbara</creatorcontrib><creatorcontrib>Morris, Mariana</creatorcontrib><creatorcontrib>Wheeler, Frances B.</creatorcontrib><title>Differential regulation of calcitonin gene-related peptide and substance P in cultured neonatal rat vagal sensory neurons</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Nodose (inferior vagal sensory) ganglia were removed from neonatal rats, enzymatically dispersed using neutral protease, and maintained on previously dispersed rat atriacytes. After 7–10 days in culture, calcitonin gene-related peptide (CGRP) was present in 1–3 times the molar amount of substance P (SP). The content of SP was doubled by the addition of nerve growth factor (NGF) whereas CGRP was significantly less increased by 50% or less. The addition of forskolin increased SP and CGRP levels in cultures with or without NGF by 60–80 percent. Phorbol ester (PMA) did not alter SP content but significantly raised CGRP content by 40% in NGF supplemented cultures (
P < 0.001). Corticosterone, reduced SP content by 30% independently of NGF but had no effect on CGRP. These studies demonstrate that SP in vagal sensory neurons is more sensitive than CGRP to the effects of NGF or corticosterone. Both peptides are up-regulated by presumed increases in intracellular cyclic AMP, while CGRP (or CGRP neurons) may be independently regulated by protein kinase C.</description><subject>Animals</subject><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Calcitonin Gene-Related Peptide</subject><subject>Cell Count</subject><subject>Cells, Cultured</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Colforsin - pharmacology</subject><subject>Corticosterone - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Nerve growth factor</subject><subject>Nerve Growth Factors - pharmacology</subject><subject>Neurons, Afferent - drug effects</subject><subject>Neurons, Afferent - metabolism</subject><subject>Neuropeptides - metabolism</subject><subject>Nodose ganglion</subject><subject>Nodose Ganglion - cytology</subject><subject>Nodose Ganglion - drug effects</subject><subject>Nodose Ganglion - metabolism</subject><subject>Phorbol Esters - pharmacology</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Substance P</subject><subject>Substance P - metabolism</subject><subject>Tissue culture</subject><subject>Vagus nerve</subject><subject>Vagus Nerve - cytology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMGOFCEQholxs46rb6AJB2P00ArdNA2XTcyqu5tsogc9EwaKCaYHRqA3mbe3xpnMUS9A5f-qUnyEvOLsA2dcfmSMyU5pPbxT-r3mIx87_oSsuJr6TvaCPSWrM_KMPK_1F5bDoNklueyFlOPQr8j-cwwBCqQW7UwLbJbZtpgTzYE6O7vYcoqJbiBBVwAz8HQHuxY9UJs8rcu6Npsc0O8UObfMbSnIJMjJtsNI2-ij3eCrQqq57DFaSk71BbkIdq7w8nRfkZ9fv_y4uesevt3e33x66JwQsnWj5kH0gg9ikoPVDKapZ3xcc6esZCqMGl2ogYvgPAQbvAvOau-kWkPgMA1X5O1x7q7k3wvUZraxOphnizsu1fCxH7VUDEFxBF3JtRYIZlfi1pa94cwcjJuDTnPQiYf5a9xwbHt9mr-st-DPTSfFmL855bai0VDQVqxnTGo5CgT_h-Hv1aQRuz5igMoeIxRTXQT072MB14zP8d_r_gHuaqty</recordid><startdate>19890130</startdate><enddate>19890130</enddate><creator>MacLean, David B.</creator><creator>Bennett, Barbara</creator><creator>Morris, Mariana</creator><creator>Wheeler, Frances B.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>19890130</creationdate><title>Differential regulation of calcitonin gene-related peptide and substance P in cultured neonatal rat vagal sensory neurons</title><author>MacLean, David B. ; Bennett, Barbara ; Morris, Mariana ; Wheeler, Frances B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-591f424134763a90e772015b1c8a608f590168314fcdefafdcfca9dc68bef1e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Calcitonin Gene-Related Peptide</topic><topic>Cell Count</topic><topic>Cells, Cultured</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Colforsin - pharmacology</topic><topic>Corticosterone - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Nerve growth factor</topic><topic>Nerve Growth Factors - pharmacology</topic><topic>Neurons, Afferent - drug effects</topic><topic>Neurons, Afferent - metabolism</topic><topic>Neuropeptides - metabolism</topic><topic>Nodose ganglion</topic><topic>Nodose Ganglion - cytology</topic><topic>Nodose Ganglion - drug effects</topic><topic>Nodose Ganglion - metabolism</topic><topic>Phorbol Esters - pharmacology</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Substance P</topic><topic>Substance P - metabolism</topic><topic>Tissue culture</topic><topic>Vagus nerve</topic><topic>Vagus Nerve - cytology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MacLean, David B.</creatorcontrib><creatorcontrib>Bennett, Barbara</creatorcontrib><creatorcontrib>Morris, Mariana</creatorcontrib><creatorcontrib>Wheeler, Frances B.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MacLean, David B.</au><au>Bennett, Barbara</au><au>Morris, Mariana</au><au>Wheeler, Frances B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential regulation of calcitonin gene-related peptide and substance P in cultured neonatal rat vagal sensory neurons</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1989-01-30</date><risdate>1989</risdate><volume>478</volume><issue>2</issue><spage>349</spage><epage>355</epage><pages>349-355</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>Nodose (inferior vagal sensory) ganglia were removed from neonatal rats, enzymatically dispersed using neutral protease, and maintained on previously dispersed rat atriacytes. After 7–10 days in culture, calcitonin gene-related peptide (CGRP) was present in 1–3 times the molar amount of substance P (SP). The content of SP was doubled by the addition of nerve growth factor (NGF) whereas CGRP was significantly less increased by 50% or less. The addition of forskolin increased SP and CGRP levels in cultures with or without NGF by 60–80 percent. Phorbol ester (PMA) did not alter SP content but significantly raised CGRP content by 40% in NGF supplemented cultures (
P < 0.001). Corticosterone, reduced SP content by 30% independently of NGF but had no effect on CGRP. These studies demonstrate that SP in vagal sensory neurons is more sensitive than CGRP to the effects of NGF or corticosterone. Both peptides are up-regulated by presumed increases in intracellular cyclic AMP, while CGRP (or CGRP neurons) may be independently regulated by protein kinase C.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2466532</pmid><doi>10.1016/0006-8993(89)91515-1</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Biochemistry and metabolism Biological and medical sciences Calcitonin Gene-Related Peptide Cell Count Cells, Cultured Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Colforsin - pharmacology Corticosterone - pharmacology Fundamental and applied biological sciences. Psychology Nerve growth factor Nerve Growth Factors - pharmacology Neurons, Afferent - drug effects Neurons, Afferent - metabolism Neuropeptides - metabolism Nodose ganglion Nodose Ganglion - cytology Nodose Ganglion - drug effects Nodose Ganglion - metabolism Phorbol Esters - pharmacology Rats Rats, Inbred Strains Substance P Substance P - metabolism Tissue culture Vagus nerve Vagus Nerve - cytology Vertebrates: nervous system and sense organs |
| title | Differential regulation of calcitonin gene-related peptide and substance P in cultured neonatal rat vagal sensory neurons |
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