Actions of robustoxin, a neurotoxic polypeptide from the venom of the male funnel-web spider (Atrax robustus), in anaesthetized monkeys
E. J. Mylecharane, I. Spence, D. D. Sheumack, R. Claassens and M. E. H. Howden. Actions of robustoxin, a neurotoxic polypeptide from the venom of the male funnel-web spider ( Atrax robustus), in anaesthetized monkeys. Toxicon 27, 481–492, 1989.—Robustoxin, a polypeptide consisting of a chain of 42 a...
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| Veröffentlicht in: | Toxicon (Oxford) 1989, Vol.27 (4), p.481-492 |
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| creator | Mylecharane, Ewan J. Spence, Ian Sheumack, David D. Claassens, Ronald Howden, Merlin E.H. |
| description | E. J.
Mylecharane, I.
Spence, D. D.
Sheumack, R.
Claassens and M. E. H.
Howden. Actions of robustoxin, a neurotoxic polypeptide from the venom of the male funnel-web spider (
Atrax robustus), in anaesthetized monkeys.
Toxicon
27, 481–492, 1989.—Robustoxin, a polypeptide consisting of a chain of 42 amino acid residues in a known sequence, has been isolated by cation exchange chromatography from the crude venom of the male funnel-web spider (
Atrax robustus). Physiological activity or toxicity in the venom fractions was detected by production of fasciculation in mouse phrenic nerve-hemidiaphragm preparations and by lethality in new-born mice. In the present experiments in
Macaca fascicularis monkeys anaesthetized with pentobarbitone, robustoxin (5–30 μg/kg infused i.v. over 5 min) produced immediate disturbances in respiration (including dyspnoea and apnoea), blood pressure and heart rate, followed by severe hypotension (mean systemic blood pressure |
| doi_str_mv | 10.1016/0041-0101(89)90211-0 |
| format | Article |
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Mylecharane, I.
Spence, D. D.
Sheumack, R.
Claassens and M. E. H.
Howden. Actions of robustoxin, a neurotoxic polypeptide from the venom of the male funnel-web spider (
Atrax robustus), in anaesthetized monkeys.
Toxicon
27, 481–492, 1989.—Robustoxin, a polypeptide consisting of a chain of 42 amino acid residues in a known sequence, has been isolated by cation exchange chromatography from the crude venom of the male funnel-web spider (
Atrax robustus). Physiological activity or toxicity in the venom fractions was detected by production of fasciculation in mouse phrenic nerve-hemidiaphragm preparations and by lethality in new-born mice. In the present experiments in
Macaca fascicularis monkeys anaesthetized with pentobarbitone, robustoxin (5–30 μg/kg infused i.v. over 5 min) produced immediate disturbances in respiration (including dyspnoea and apnoea), blood pressure and heart rate, followed by severe hypotension (mean systemic blood pressure <50 mmHg) or death due to respiratory and circulatory failure within 196 min. Robustoxin also produced lachrymation, salivation, generalized skeletal muscle fasciculation and a parallel increase in body temperature, and increased firing in skeletal motor and autonomic nerves. These effects closely resembled those produced by i.v. infusions over 5 min of 50 μg/kg of crude venom from male
A. robustus spiders. Crude venom from female
A. robustus spiders (500 μg/kg i.v. over 5 min) produced some of the effects elicited by robustoxin and crude venom from male spiders, but to a much less marked extent. It was concluded that robustoxin is responsible for the neurotoxic and lethal effects of human envenomation by male
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Mylecharane, I.
Spence, D. D.
Sheumack, R.
Claassens and M. E. H.
Howden. Actions of robustoxin, a neurotoxic polypeptide from the venom of the male funnel-web spider (
Atrax robustus), in anaesthetized monkeys.
Toxicon
27, 481–492, 1989.—Robustoxin, a polypeptide consisting of a chain of 42 amino acid residues in a known sequence, has been isolated by cation exchange chromatography from the crude venom of the male funnel-web spider (
Atrax robustus). Physiological activity or toxicity in the venom fractions was detected by production of fasciculation in mouse phrenic nerve-hemidiaphragm preparations and by lethality in new-born mice. In the present experiments in
Macaca fascicularis monkeys anaesthetized with pentobarbitone, robustoxin (5–30 μg/kg infused i.v. over 5 min) produced immediate disturbances in respiration (including dyspnoea and apnoea), blood pressure and heart rate, followed by severe hypotension (mean systemic blood pressure <50 mmHg) or death due to respiratory and circulatory failure within 196 min. Robustoxin also produced lachrymation, salivation, generalized skeletal muscle fasciculation and a parallel increase in body temperature, and increased firing in skeletal motor and autonomic nerves. These effects closely resembled those produced by i.v. infusions over 5 min of 50 μg/kg of crude venom from male
A. robustus spiders. Crude venom from female
A. robustus spiders (500 μg/kg i.v. over 5 min) produced some of the effects elicited by robustoxin and crude venom from male spiders, but to a much less marked extent. It was concluded that robustoxin is responsible for the neurotoxic and lethal effects of human envenomation by male
A. robustus spiders.</description><subject>Anesthesia</subject><subject>Animal poisons toxicology. Antivenoms</subject><subject>Animals</subject><subject>Arthropod Venoms - toxicity</subject><subject>Biological and medical sciences</subject><subject>Blood Pressure - drug effects</subject><subject>Crying - physiology</subject><subject>Electromyography</subject><subject>Female</subject><subject>Heart Rate - drug effects</subject><subject>Hexathelidae</subject><subject>Injections, Intramuscular</subject><subject>Injections, Intravenous</subject><subject>Macaca fascicularis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Salivation - drug effects</subject><subject>Spider Venoms - toxicity</subject><subject>Toxicology</subject><issn>0041-0101</issn><issn>1879-3150</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV9LHDEUxYNUdN36DRTyUIrCTk3mb_JSWBZbBcEX-xySzB2aOpOMyYzd9Qv4tZvpDvvoU-7h_s7hcoLQBSXfKKHlDSE5TUgcrxi_5iSlUR2hBWUVTzJakE9ocUBO0VkIfwghGePlCTpJq5SRLFug97UejLMBuwZ7p8YwuK2xKyyxhdG7SWncu3bXQz-YGnDjXYeH34BfwcYp2ibRyTauRmuhTf6CwqGPrMdX68HL7Rw8husVNhZLKyFE02DeoMads8-wC5_RcSPbAOfzu0S_ftw-be6Sh8ef95v1Q6JzWg2Jlg0oBgXLc8JVw8uSUa5KnmulqMpqQijLq0anpQJW0QjJLFeyplxntZY0W6Kv-9zeu5cx3iE6EzS0rbTgxiBokRasqtII5ntQexeCh0b03nTS7wQlYupfTOWKqVzBuPjfvyDRdjnnj6qD-mCaC4_7L_NeBi3bxkurTThgZVHFT5rO_L7HIHbxasCLoA1YDbXxoAdRO_PxHf8A1YWjGQ</recordid><startdate>1989</startdate><enddate>1989</enddate><creator>Mylecharane, Ewan J.</creator><creator>Spence, Ian</creator><creator>Sheumack, David D.</creator><creator>Claassens, Ronald</creator><creator>Howden, Merlin E.H.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TK</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>1989</creationdate><title>Actions of robustoxin, a neurotoxic polypeptide from the venom of the male funnel-web spider (Atrax robustus), in anaesthetized monkeys</title><author>Mylecharane, Ewan J. ; Spence, Ian ; Sheumack, David D. ; Claassens, Ronald ; Howden, Merlin E.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-cafeb8e584409bf966819b694cbb1b3d001847fc26be871409a34bad19c3dca13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Anesthesia</topic><topic>Animal poisons toxicology. Antivenoms</topic><topic>Animals</topic><topic>Arthropod Venoms - toxicity</topic><topic>Biological and medical sciences</topic><topic>Blood Pressure - drug effects</topic><topic>Crying - physiology</topic><topic>Electromyography</topic><topic>Female</topic><topic>Heart Rate - drug effects</topic><topic>Hexathelidae</topic><topic>Injections, Intramuscular</topic><topic>Injections, Intravenous</topic><topic>Macaca fascicularis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Salivation - drug effects</topic><topic>Spider Venoms - toxicity</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mylecharane, Ewan J.</creatorcontrib><creatorcontrib>Spence, Ian</creatorcontrib><creatorcontrib>Sheumack, David D.</creatorcontrib><creatorcontrib>Claassens, Ronald</creatorcontrib><creatorcontrib>Howden, Merlin E.H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicon (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mylecharane, Ewan J.</au><au>Spence, Ian</au><au>Sheumack, David D.</au><au>Claassens, Ronald</au><au>Howden, Merlin E.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Actions of robustoxin, a neurotoxic polypeptide from the venom of the male funnel-web spider (Atrax robustus), in anaesthetized monkeys</atitle><jtitle>Toxicon (Oxford)</jtitle><addtitle>Toxicon</addtitle><date>1989</date><risdate>1989</risdate><volume>27</volume><issue>4</issue><spage>481</spage><epage>492</epage><pages>481-492</pages><issn>0041-0101</issn><eissn>1879-3150</eissn><coden>TOXIA6</coden><abstract>E. J.
Mylecharane, I.
Spence, D. D.
Sheumack, R.
Claassens and M. E. H.
Howden. Actions of robustoxin, a neurotoxic polypeptide from the venom of the male funnel-web spider (
Atrax robustus), in anaesthetized monkeys.
Toxicon
27, 481–492, 1989.—Robustoxin, a polypeptide consisting of a chain of 42 amino acid residues in a known sequence, has been isolated by cation exchange chromatography from the crude venom of the male funnel-web spider (
Atrax robustus). Physiological activity or toxicity in the venom fractions was detected by production of fasciculation in mouse phrenic nerve-hemidiaphragm preparations and by lethality in new-born mice. In the present experiments in
Macaca fascicularis monkeys anaesthetized with pentobarbitone, robustoxin (5–30 μg/kg infused i.v. over 5 min) produced immediate disturbances in respiration (including dyspnoea and apnoea), blood pressure and heart rate, followed by severe hypotension (mean systemic blood pressure <50 mmHg) or death due to respiratory and circulatory failure within 196 min. Robustoxin also produced lachrymation, salivation, generalized skeletal muscle fasciculation and a parallel increase in body temperature, and increased firing in skeletal motor and autonomic nerves. These effects closely resembled those produced by i.v. infusions over 5 min of 50 μg/kg of crude venom from male
A. robustus spiders. Crude venom from female
A. robustus spiders (500 μg/kg i.v. over 5 min) produced some of the effects elicited by robustoxin and crude venom from male spiders, but to a much less marked extent. It was concluded that robustoxin is responsible for the neurotoxic and lethal effects of human envenomation by male
A. robustus spiders.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>2728033</pmid><doi>10.1016/0041-0101(89)90211-0</doi><tpages>12</tpages></addata></record> |
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| ispartof | Toxicon (Oxford), 1989, Vol.27 (4), p.481-492 |
| issn | 0041-0101 1879-3150 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Anesthesia Animal poisons toxicology. Antivenoms Animals Arthropod Venoms - toxicity Biological and medical sciences Blood Pressure - drug effects Crying - physiology Electromyography Female Heart Rate - drug effects Hexathelidae Injections, Intramuscular Injections, Intravenous Macaca fascicularis Male Medical sciences Salivation - drug effects Spider Venoms - toxicity Toxicology |
| title | Actions of robustoxin, a neurotoxic polypeptide from the venom of the male funnel-web spider (Atrax robustus), in anaesthetized monkeys |
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