Engineering complex biological systems in bacteria through recombinase-assisted genome engineering

A protocol enabling the production of biofuels and renewable chemicals via the design and construction of complex biological systems in microbial organisms. Here we describe an advanced paradigm for the design, construction and stable implementation of complex biological systems in microbial organis...

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Veröffentlicht in:	Nature protocols 2014-06, Vol.9 (6), p.1320-1336
Hauptverfasser:	Santos, Christine Nicole S, Yoshikuni, Yasuo
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/338/318 631/326/41/2173 631/449/906 631/553/2690 Algae Analytical Chemistry Artificial chromosomes Bacteria - genetics Bacterial artificial chromosomes Biodiesel fuels Biofuels Biological Techniques Chromosomes Chromosomes, Artificial, Bacterial - genetics Computational Biology/Bioinformatics Copy number Design E coli Engineering Genetic engineering Genetic Engineering - methods Genetic transformation Genetic vectors Genome editing Genome, Bacterial - genetics Genomes Integration Life Sciences Metabolic Networks and Pathways - genetics Methods Microarrays Microorganisms Modules Optimization Organic Chemistry Physiological aspects Plasmids Protocol Raw materials Recombinase Recombinases - metabolism Seaweeds Strain Trouble shooting
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