Antioxidant and antimicrobial properties of phenolic rich fraction of Seabuckthorn (Hippophae rhamnoides L.) leaves in vitro
•Preparation of phenolic rich fraction from Seabuckthorn leaves.•Evaluation of antioxidant and antimicrobial activities.•A simple RP-HPLC method for the quantitative analysis of phenolic compounds. Phenolic rich fraction (PRF) from Seabuckthorn leaves was prepared by sequential fractionation. Total...
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| Veröffentlicht in: | Food chemistry 2013-12, Vol.141 (4), p.3443-3450 |
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| creator | Yogendra Kumar, M.S. Tirpude, R.J. Maheshwari, D.T. Bansal, Anju Misra, Ksipra |
| description | •Preparation of phenolic rich fraction from Seabuckthorn leaves.•Evaluation of antioxidant and antimicrobial activities.•A simple RP-HPLC method for the quantitative analysis of phenolic compounds.
Phenolic rich fraction (PRF) from Seabuckthorn leaves was prepared by sequential fractionation. Total phenolic content of PRF estimated as gallic acid equivalent was found to be 319.33±7.02mg/g of PRF. Its major constituents gallic acid, rutin, quercetin-3-galactoside, quercetin-3-glucoside, myricetin, quercetin, kaempferol and isorhamnetin, were found in the range of 1.551–196.89mg/g of PRF as determined by RP-HPLC. Antioxidant activity of PRF evaluated using 2,2-diphenyl-2-picrylhydrazyl, superoxide and nitric oxide scavenging assays. Reducing power of PRF increased with increasing amount of PRF; the equation of reducing power (y) and amount of PRF (x) was y=8.004x (r2=0.99), indicating that reducing ability correlated well with amount of PRF.
Antibacterial activity of PRF, tested against certain medically important bacterial species showed growth inhibiting effect against Escherichia coli, Salmonella typhi, Shigella dysenteriae, Streptococcus pneumoniae and Staphylococcus aureus. In conclusion, PRF has potent antioxidant and broad spectrum antibacterial properties. |
| doi_str_mv | 10.1016/j.foodchem.2013.06.057 |
| format | Article |
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Phenolic rich fraction (PRF) from Seabuckthorn leaves was prepared by sequential fractionation. Total phenolic content of PRF estimated as gallic acid equivalent was found to be 319.33±7.02mg/g of PRF. Its major constituents gallic acid, rutin, quercetin-3-galactoside, quercetin-3-glucoside, myricetin, quercetin, kaempferol and isorhamnetin, were found in the range of 1.551–196.89mg/g of PRF as determined by RP-HPLC. Antioxidant activity of PRF evaluated using 2,2-diphenyl-2-picrylhydrazyl, superoxide and nitric oxide scavenging assays. Reducing power of PRF increased with increasing amount of PRF; the equation of reducing power (y) and amount of PRF (x) was y=8.004x (r2=0.99), indicating that reducing ability correlated well with amount of PRF.
Antibacterial activity of PRF, tested against certain medically important bacterial species showed growth inhibiting effect against Escherichia coli, Salmonella typhi, Shigella dysenteriae, Streptococcus pneumoniae and Staphylococcus aureus. In conclusion, PRF has potent antioxidant and broad spectrum antibacterial properties.</description><identifier>ISSN: 0308-8146</identifier><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2013.06.057</identifier><identifier>PMID: 23993505</identifier><identifier>CODEN: FOCHDJ</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - isolation & purification ; Anti-Bacterial Agents - pharmacology ; Antibacterial agents ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antimicrobial activity ; Antioxidant ; Antioxidants - chemistry ; Antioxidants - isolation & purification ; Antioxidants - pharmacology ; Bacteria - drug effects ; Bacteria - growth & development ; Biological and medical sciences ; Hippophae - chemistry ; Hippophae rhamnoides ; HPLC ; Medical sciences ; Pharmacology. Drug treatments ; Phenol ; Phenols - chemistry ; Phenols - isolation & purification ; Phenols - pharmacology ; Plant Extracts - chemistry ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; Plant Leaves - chemistry ; Seabuckthorn</subject><ispartof>Food chemistry, 2013-12, Vol.141 (4), p.3443-3450</ispartof><rights>2013 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2013 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-c88e9a17ca1ec9c01fc5c982728db2de351e24fefb31530b2dac00dea9181a943</citedby><cites>FETCH-LOGICAL-c497t-c88e9a17ca1ec9c01fc5c982728db2de351e24fefb31530b2dac00dea9181a943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.foodchem.2013.06.057$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3554,27933,27934,46004</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27713028$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23993505$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yogendra Kumar, M.S.</creatorcontrib><creatorcontrib>Tirpude, R.J.</creatorcontrib><creatorcontrib>Maheshwari, D.T.</creatorcontrib><creatorcontrib>Bansal, Anju</creatorcontrib><creatorcontrib>Misra, Ksipra</creatorcontrib><title>Antioxidant and antimicrobial properties of phenolic rich fraction of Seabuckthorn (Hippophae rhamnoides L.) leaves in vitro</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>•Preparation of phenolic rich fraction from Seabuckthorn leaves.•Evaluation of antioxidant and antimicrobial activities.•A simple RP-HPLC method for the quantitative analysis of phenolic compounds.
Phenolic rich fraction (PRF) from Seabuckthorn leaves was prepared by sequential fractionation. Total phenolic content of PRF estimated as gallic acid equivalent was found to be 319.33±7.02mg/g of PRF. Its major constituents gallic acid, rutin, quercetin-3-galactoside, quercetin-3-glucoside, myricetin, quercetin, kaempferol and isorhamnetin, were found in the range of 1.551–196.89mg/g of PRF as determined by RP-HPLC. Antioxidant activity of PRF evaluated using 2,2-diphenyl-2-picrylhydrazyl, superoxide and nitric oxide scavenging assays. Reducing power of PRF increased with increasing amount of PRF; the equation of reducing power (y) and amount of PRF (x) was y=8.004x (r2=0.99), indicating that reducing ability correlated well with amount of PRF.
Antibacterial activity of PRF, tested against certain medically important bacterial species showed growth inhibiting effect against Escherichia coli, Salmonella typhi, Shigella dysenteriae, Streptococcus pneumoniae and Staphylococcus aureus. In conclusion, PRF has potent antioxidant and broad spectrum antibacterial properties.</description><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - isolation & purification</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibacterial agents</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antimicrobial activity</subject><subject>Antioxidant</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - isolation & purification</subject><subject>Antioxidants - pharmacology</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - growth & development</subject><subject>Biological and medical sciences</subject><subject>Hippophae - chemistry</subject><subject>Hippophae rhamnoides</subject><subject>HPLC</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Phenol</subject><subject>Phenols - chemistry</subject><subject>Phenols - isolation & purification</subject><subject>Phenols - pharmacology</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Leaves - chemistry</subject><subject>Seabuckthorn</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFv1DAQhS0EokvhL1S5IJVDwthOYudGVdEWaSUOwNly7IniJbGDnV0ViR-PV7uFIxwsW_b3xjPvEXJFoaJA2_e7agjBmhHnigHlFbQVNOIZ2VApeClAsOdkAxxkKWndXpBXKe0AILPyJblgvOt4A82G_LrxqwuPzmq_FtrbvFY3OxND7_RULDEsGFeHqQhDsYzow-RMEZ0ZiyFqk7X--PIFdb8339cxRF9cP7hlCcuosYijnn1wNuu31btiQn3IR-eLg1tjeE1eDHpK-Oa8X5Jvdx-_3j6U28_3n25vtqWpO7GWRkrsNBVGUzSdATqYxnSSCSZtzyzyhiKrBxx6ThsO-UobAIu6o5LqruaX5PpUN4_zY49pVbNLBqdJewz7pGjD6poBa_8DrVnX1iDFEW1PaDYrpYiDWqKbdfypKKhjSGqnnkJSx5AUtCqHlIVX5z_2_Yz2j-wplQy8PQM6GT1lo71x6S8nBOXAZOY-nDjM5h0cRpWMQ2_QuohmVTa4f_XyGyUFtSY</recordid><startdate>20131215</startdate><enddate>20131215</enddate><creator>Yogendra Kumar, M.S.</creator><creator>Tirpude, R.J.</creator><creator>Maheshwari, D.T.</creator><creator>Bansal, Anju</creator><creator>Misra, Ksipra</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20131215</creationdate><title>Antioxidant and antimicrobial properties of phenolic rich fraction of Seabuckthorn (Hippophae rhamnoides L.) leaves in vitro</title><author>Yogendra Kumar, M.S. ; Tirpude, R.J. ; Maheshwari, D.T. ; Bansal, Anju ; Misra, Ksipra</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c497t-c88e9a17ca1ec9c01fc5c982728db2de351e24fefb31530b2dac00dea9181a943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - isolation & purification</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibacterial agents</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Antimicrobial activity</topic><topic>Antioxidant</topic><topic>Antioxidants - chemistry</topic><topic>Antioxidants - isolation & purification</topic><topic>Antioxidants - pharmacology</topic><topic>Bacteria - drug effects</topic><topic>Bacteria - growth & development</topic><topic>Biological and medical sciences</topic><topic>Hippophae - chemistry</topic><topic>Hippophae rhamnoides</topic><topic>HPLC</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Phenol</topic><topic>Phenols - chemistry</topic><topic>Phenols - isolation & purification</topic><topic>Phenols - pharmacology</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Leaves - chemistry</topic><topic>Seabuckthorn</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yogendra Kumar, M.S.</creatorcontrib><creatorcontrib>Tirpude, R.J.</creatorcontrib><creatorcontrib>Maheshwari, D.T.</creatorcontrib><creatorcontrib>Bansal, Anju</creatorcontrib><creatorcontrib>Misra, Ksipra</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yogendra Kumar, M.S.</au><au>Tirpude, R.J.</au><au>Maheshwari, D.T.</au><au>Bansal, Anju</au><au>Misra, Ksipra</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antioxidant and antimicrobial properties of phenolic rich fraction of Seabuckthorn (Hippophae rhamnoides L.) leaves in vitro</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2013-12-15</date><risdate>2013</risdate><volume>141</volume><issue>4</issue><spage>3443</spage><epage>3450</epage><pages>3443-3450</pages><issn>0308-8146</issn><eissn>1873-7072</eissn><coden>FOCHDJ</coden><abstract>•Preparation of phenolic rich fraction from Seabuckthorn leaves.•Evaluation of antioxidant and antimicrobial activities.•A simple RP-HPLC method for the quantitative analysis of phenolic compounds.
Phenolic rich fraction (PRF) from Seabuckthorn leaves was prepared by sequential fractionation. Total phenolic content of PRF estimated as gallic acid equivalent was found to be 319.33±7.02mg/g of PRF. Its major constituents gallic acid, rutin, quercetin-3-galactoside, quercetin-3-glucoside, myricetin, quercetin, kaempferol and isorhamnetin, were found in the range of 1.551–196.89mg/g of PRF as determined by RP-HPLC. Antioxidant activity of PRF evaluated using 2,2-diphenyl-2-picrylhydrazyl, superoxide and nitric oxide scavenging assays. Reducing power of PRF increased with increasing amount of PRF; the equation of reducing power (y) and amount of PRF (x) was y=8.004x (r2=0.99), indicating that reducing ability correlated well with amount of PRF.
Antibacterial activity of PRF, tested against certain medically important bacterial species showed growth inhibiting effect against Escherichia coli, Salmonella typhi, Shigella dysenteriae, Streptococcus pneumoniae and Staphylococcus aureus. In conclusion, PRF has potent antioxidant and broad spectrum antibacterial properties.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>23993505</pmid><doi>10.1016/j.foodchem.2013.06.057</doi><tpages>8</tpages></addata></record> |
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| subjects | Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - isolation & purification Anti-Bacterial Agents - pharmacology Antibacterial agents Antibiotics. Antiinfectious agents. Antiparasitic agents Antimicrobial activity Antioxidant Antioxidants - chemistry Antioxidants - isolation & purification Antioxidants - pharmacology Bacteria - drug effects Bacteria - growth & development Biological and medical sciences Hippophae - chemistry Hippophae rhamnoides HPLC Medical sciences Pharmacology. Drug treatments Phenol Phenols - chemistry Phenols - isolation & purification Phenols - pharmacology Plant Extracts - chemistry Plant Extracts - isolation & purification Plant Extracts - pharmacology Plant Leaves - chemistry Seabuckthorn |
| title | Antioxidant and antimicrobial properties of phenolic rich fraction of Seabuckthorn (Hippophae rhamnoides L.) leaves in vitro |
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