Arabidopsis AtPAP1 transcription factor induces anthocyanin production in transgenic Taraxacum brevicorniculatum
KEY MESSAGE : This study developed a new purple coloured Taraxacum brevicorniculatum plant through genetic transformation using the Arabidopsis AtPAP1 gene, which overproduced anthocyanins in its vegetative tissues. Rubber-producing Taraxacum plants synthesise high-quality natural rubber (NR) in the...
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| Veröffentlicht in: | Plant cell reports 2014-04, Vol.33 (4), p.669-680 |
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| creator | Qiu, Jian Sun, Shuquan Luo, Shiqiao Zhang, Jichuan Xiao, Xianzhou Zhang, Liqun Wang, Feng Liu, Shizhong |
| description | KEY MESSAGE : This study developed a new purple coloured Taraxacum brevicorniculatum plant through genetic transformation using the Arabidopsis AtPAP1 gene, which overproduced anthocyanins in its vegetative tissues. Rubber-producing Taraxacum plants synthesise high-quality natural rubber (NR) in their roots and so are a promising alternative global source of this raw material. A major factor in its commercialization is the need for multipurpose exploitation of the whole plant. To add value to the aerial tissues, red/purple plants of the rubber-producing Taraxacum brevicorniculatum species were developed through heterologous expression of the production of anthocyanin pigment 1 (AtPAP1) transcription factor from Arabidopsis thaliana. The vegetative tissue of the transgenic plants showed an average of a 48-fold increase in total anthocyanin content over control levels, but with the exception of pigmentation, the transgenic plants were phenotypically comparable to controls and displayed similar growth vigor. Southern blot analysis confirmed that the AtPAP1 gene had been integrated into the genome of the high anthocyanin Taraxacum plants. The AtPAP1 expression levels were estimated by quantitative real-time PCR and were highly correlated with the levels of total anthocyanins in five independent transgenic lines. High levels of three cyanidin glycosides found in the purple plants were characterized by high performance liquid chromatography–mass spectrum analysis. The presence of NR was verified by NMR and infrared spectroscopy, and confirmed that NR biosynthesis had not been affected in the transgenic Taraxacum lines. In addition, other major phenylpropanoid products such as chlorogenic acid and quercetin glycosides were also enhanced in the transgenic Taraxacum. The red/purple transgenic Taraxacum lines described in this study would increase the future application of the species as a rubber-producing crop due to its additional health benefits. |
| doi_str_mv | 10.1007/s00299-014-1585-8 |
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Rubber-producing Taraxacum plants synthesise high-quality natural rubber (NR) in their roots and so are a promising alternative global source of this raw material. A major factor in its commercialization is the need for multipurpose exploitation of the whole plant. To add value to the aerial tissues, red/purple plants of the rubber-producing Taraxacum brevicorniculatum species were developed through heterologous expression of the production of anthocyanin pigment 1 (AtPAP1) transcription factor from Arabidopsis thaliana. The vegetative tissue of the transgenic plants showed an average of a 48-fold increase in total anthocyanin content over control levels, but with the exception of pigmentation, the transgenic plants were phenotypically comparable to controls and displayed similar growth vigor. Southern blot analysis confirmed that the AtPAP1 gene had been integrated into the genome of the high anthocyanin Taraxacum plants. The AtPAP1 expression levels were estimated by quantitative real-time PCR and were highly correlated with the levels of total anthocyanins in five independent transgenic lines. High levels of three cyanidin glycosides found in the purple plants were characterized by high performance liquid chromatography–mass spectrum analysis. The presence of NR was verified by NMR and infrared spectroscopy, and confirmed that NR biosynthesis had not been affected in the transgenic Taraxacum lines. In addition, other major phenylpropanoid products such as chlorogenic acid and quercetin glycosides were also enhanced in the transgenic Taraxacum. The red/purple transgenic Taraxacum lines described in this study would increase the future application of the species as a rubber-producing crop due to its additional health benefits.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-014-1585-8</identifier><identifier>PMID: 24556963</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>anthocyanins ; Anthocyanins - biosynthesis ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; Biomedical and Life Sciences ; Biosynthesis ; Biotechnology ; Blotting, Southern ; Cell Biology ; chlorogenic acid ; commercialization ; Gene Expression Regulation, Plant ; genes ; genetic transformation ; Infrared spectroscopy ; Life Sciences ; Liquid chromatography ; nuclear magnetic resonance spectroscopy ; Original Paper ; Phenotype ; Pigmentation ; Plant Biochemistry ; Plant Sciences ; Plants, Genetically Modified ; Polymerase Chain Reaction ; Propanols - metabolism ; quantitative polymerase chain reaction ; raw materials ; roots ; Rubber ; Southern blotting ; Taraxacum ; Taraxacum - genetics ; Taraxacum - metabolism ; transcription factors ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transformation, Genetic ; Transgenic plants ; vigor</subject><ispartof>Plant cell reports, 2014-04, Vol.33 (4), p.669-680</ispartof><rights>Springer-Verlag Berlin Heidelberg 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-ac270f36117f99c3a8284b51b15e82ea4006ed1e3b6248ccfa4b050e224c1d5e3</citedby><cites>FETCH-LOGICAL-c429t-ac270f36117f99c3a8284b51b15e82ea4006ed1e3b6248ccfa4b050e224c1d5e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00299-014-1585-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00299-014-1585-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24556963$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qiu, Jian</creatorcontrib><creatorcontrib>Sun, Shuquan</creatorcontrib><creatorcontrib>Luo, Shiqiao</creatorcontrib><creatorcontrib>Zhang, Jichuan</creatorcontrib><creatorcontrib>Xiao, Xianzhou</creatorcontrib><creatorcontrib>Zhang, Liqun</creatorcontrib><creatorcontrib>Wang, Feng</creatorcontrib><creatorcontrib>Liu, Shizhong</creatorcontrib><title>Arabidopsis AtPAP1 transcription factor induces anthocyanin production in transgenic Taraxacum brevicorniculatum</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><addtitle>Plant Cell Rep</addtitle><description>KEY MESSAGE : This study developed a new purple coloured Taraxacum brevicorniculatum plant through genetic transformation using the Arabidopsis AtPAP1 gene, which overproduced anthocyanins in its vegetative tissues. Rubber-producing Taraxacum plants synthesise high-quality natural rubber (NR) in their roots and so are a promising alternative global source of this raw material. A major factor in its commercialization is the need for multipurpose exploitation of the whole plant. To add value to the aerial tissues, red/purple plants of the rubber-producing Taraxacum brevicorniculatum species were developed through heterologous expression of the production of anthocyanin pigment 1 (AtPAP1) transcription factor from Arabidopsis thaliana. The vegetative tissue of the transgenic plants showed an average of a 48-fold increase in total anthocyanin content over control levels, but with the exception of pigmentation, the transgenic plants were phenotypically comparable to controls and displayed similar growth vigor. Southern blot analysis confirmed that the AtPAP1 gene had been integrated into the genome of the high anthocyanin Taraxacum plants. The AtPAP1 expression levels were estimated by quantitative real-time PCR and were highly correlated with the levels of total anthocyanins in five independent transgenic lines. High levels of three cyanidin glycosides found in the purple plants were characterized by high performance liquid chromatography–mass spectrum analysis. The presence of NR was verified by NMR and infrared spectroscopy, and confirmed that NR biosynthesis had not been affected in the transgenic Taraxacum lines. In addition, other major phenylpropanoid products such as chlorogenic acid and quercetin glycosides were also enhanced in the transgenic Taraxacum. The red/purple transgenic Taraxacum lines described in this study would increase the future application of the species as a rubber-producing crop due to its additional health benefits.</description><subject>anthocyanins</subject><subject>Anthocyanins - biosynthesis</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Blotting, Southern</subject><subject>Cell Biology</subject><subject>chlorogenic acid</subject><subject>commercialization</subject><subject>Gene Expression Regulation, Plant</subject><subject>genes</subject><subject>genetic transformation</subject><subject>Infrared spectroscopy</subject><subject>Life Sciences</subject><subject>Liquid chromatography</subject><subject>nuclear magnetic resonance spectroscopy</subject><subject>Original Paper</subject><subject>Phenotype</subject><subject>Pigmentation</subject><subject>Plant Biochemistry</subject><subject>Plant Sciences</subject><subject>Plants, Genetically Modified</subject><subject>Polymerase Chain Reaction</subject><subject>Propanols - metabolism</subject><subject>quantitative polymerase chain reaction</subject><subject>raw materials</subject><subject>roots</subject><subject>Rubber</subject><subject>Southern blotting</subject><subject>Taraxacum</subject><subject>Taraxacum - genetics</subject><subject>Taraxacum - metabolism</subject><subject>transcription factors</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transformation, Genetic</subject><subject>Transgenic plants</subject><subject>vigor</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU1rFTEUhoNY7LX6A9zoQDdupubkY5JZXopVoWDBFtyFM5nMNeVOMiYzxf57cztVxIW4Cjl53jcJDyGvgJ4BpepdppS1bU1B1CC1rPUTsgHBWc0o__qUbKhiUCsF4pg8z_mWFpCr5hk5ZkLKpm34hkzbhJ3v45R9rrbz1fYKqjlhyDb5afYxVAPaOabKh36xLlcY5m_R3mPwoZpSLMMHquweYjsXvK2uMeEPtMtYdcndeRtTmS57nJfxBTkacJ_dy8f1hNxcvL8-_1hffv7w6Xx7WVvB2rlGyxQdeAOghra1HDXTopPQgXSaORSUNq4Hx7uGCW3tgKKjkjrGhIVeOn5C3q695ZHfF5dnM_ps3X6PwcUlG5BMCFBCif9AQQguhW4KevoXehuXFMpHDhRrtFBKFgpWyqaYc3KDmZIfMd0boOZgzqzmTBFiDuaMLpnXj81LN7r-d-KXqgKwFcjlKOxc-uPqf7S-WUMDRoO75LO5-cIKQAvfKsn4T6O5rc8</recordid><startdate>20140401</startdate><enddate>20140401</enddate><creator>Qiu, Jian</creator><creator>Sun, Shuquan</creator><creator>Luo, Shiqiao</creator><creator>Zhang, Jichuan</creator><creator>Xiao, Xianzhou</creator><creator>Zhang, Liqun</creator><creator>Wang, Feng</creator><creator>Liu, Shizhong</creator><general>Springer-Verlag</general><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><scope>7QO</scope></search><sort><creationdate>20140401</creationdate><title>Arabidopsis AtPAP1 transcription factor induces anthocyanin production in transgenic Taraxacum brevicorniculatum</title><author>Qiu, Jian ; Sun, Shuquan ; Luo, Shiqiao ; Zhang, Jichuan ; Xiao, Xianzhou ; Zhang, Liqun ; Wang, Feng ; Liu, Shizhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-ac270f36117f99c3a8284b51b15e82ea4006ed1e3b6248ccfa4b050e224c1d5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>anthocyanins</topic><topic>Anthocyanins - biosynthesis</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Blotting, Southern</topic><topic>Cell Biology</topic><topic>chlorogenic acid</topic><topic>commercialization</topic><topic>Gene Expression Regulation, Plant</topic><topic>genes</topic><topic>genetic transformation</topic><topic>Infrared spectroscopy</topic><topic>Life Sciences</topic><topic>Liquid chromatography</topic><topic>nuclear magnetic resonance spectroscopy</topic><topic>Original Paper</topic><topic>Phenotype</topic><topic>Pigmentation</topic><topic>Plant Biochemistry</topic><topic>Plant Sciences</topic><topic>Plants, Genetically Modified</topic><topic>Polymerase Chain Reaction</topic><topic>Propanols - metabolism</topic><topic>quantitative polymerase chain reaction</topic><topic>raw materials</topic><topic>roots</topic><topic>Rubber</topic><topic>Southern blotting</topic><topic>Taraxacum</topic><topic>Taraxacum - genetics</topic><topic>Taraxacum - metabolism</topic><topic>transcription factors</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transformation, Genetic</topic><topic>Transgenic plants</topic><topic>vigor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qiu, Jian</creatorcontrib><creatorcontrib>Sun, Shuquan</creatorcontrib><creatorcontrib>Luo, Shiqiao</creatorcontrib><creatorcontrib>Zhang, Jichuan</creatorcontrib><creatorcontrib>Xiao, Xianzhou</creatorcontrib><creatorcontrib>Zhang, Liqun</creatorcontrib><creatorcontrib>Wang, Feng</creatorcontrib><creatorcontrib>Liu, Shizhong</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qiu, Jian</au><au>Sun, Shuquan</au><au>Luo, Shiqiao</au><au>Zhang, Jichuan</au><au>Xiao, Xianzhou</au><au>Zhang, Liqun</au><au>Wang, Feng</au><au>Liu, Shizhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Arabidopsis AtPAP1 transcription factor induces anthocyanin production in transgenic Taraxacum brevicorniculatum</atitle><jtitle>Plant cell reports</jtitle><stitle>Plant Cell Rep</stitle><addtitle>Plant Cell Rep</addtitle><date>2014-04-01</date><risdate>2014</risdate><volume>33</volume><issue>4</issue><spage>669</spage><epage>680</epage><pages>669-680</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><abstract>KEY MESSAGE : This study developed a new purple coloured Taraxacum brevicorniculatum plant through genetic transformation using the Arabidopsis AtPAP1 gene, which overproduced anthocyanins in its vegetative tissues. Rubber-producing Taraxacum plants synthesise high-quality natural rubber (NR) in their roots and so are a promising alternative global source of this raw material. A major factor in its commercialization is the need for multipurpose exploitation of the whole plant. To add value to the aerial tissues, red/purple plants of the rubber-producing Taraxacum brevicorniculatum species were developed through heterologous expression of the production of anthocyanin pigment 1 (AtPAP1) transcription factor from Arabidopsis thaliana. The vegetative tissue of the transgenic plants showed an average of a 48-fold increase in total anthocyanin content over control levels, but with the exception of pigmentation, the transgenic plants were phenotypically comparable to controls and displayed similar growth vigor. Southern blot analysis confirmed that the AtPAP1 gene had been integrated into the genome of the high anthocyanin Taraxacum plants. The AtPAP1 expression levels were estimated by quantitative real-time PCR and were highly correlated with the levels of total anthocyanins in five independent transgenic lines. High levels of three cyanidin glycosides found in the purple plants were characterized by high performance liquid chromatography–mass spectrum analysis. The presence of NR was verified by NMR and infrared spectroscopy, and confirmed that NR biosynthesis had not been affected in the transgenic Taraxacum lines. In addition, other major phenylpropanoid products such as chlorogenic acid and quercetin glycosides were also enhanced in the transgenic Taraxacum. The red/purple transgenic Taraxacum lines described in this study would increase the future application of the species as a rubber-producing crop due to its additional health benefits.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>24556963</pmid><doi>10.1007/s00299-014-1585-8</doi><tpages>12</tpages></addata></record> |
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| ispartof | Plant cell reports, 2014-04, Vol.33 (4), p.669-680 |
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| subjects | anthocyanins Anthocyanins - biosynthesis Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana Biomedical and Life Sciences Biosynthesis Biotechnology Blotting, Southern Cell Biology chlorogenic acid commercialization Gene Expression Regulation, Plant genes genetic transformation Infrared spectroscopy Life Sciences Liquid chromatography nuclear magnetic resonance spectroscopy Original Paper Phenotype Pigmentation Plant Biochemistry Plant Sciences Plants, Genetically Modified Polymerase Chain Reaction Propanols - metabolism quantitative polymerase chain reaction raw materials roots Rubber Southern blotting Taraxacum Taraxacum - genetics Taraxacum - metabolism transcription factors Transcription Factors - genetics Transcription Factors - metabolism Transformation, Genetic Transgenic plants vigor |
| title | Arabidopsis AtPAP1 transcription factor induces anthocyanin production in transgenic Taraxacum brevicorniculatum |
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