Hepatitis B virus and hepatitis D virus in blood donors from Argentina: circulation of HBsAg and reverse transcriptase mutants
In Argentina, current procedures to ensure the safety of the blood supply for transfusion include the serologic detection of specific blood-borne infections. The aim of this study was to evaluate the prevalence and the genetic diversity of hepatitis B virus (HBV) and hepatitis D virus (HDV) in blood...
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| Veröffentlicht in: | Archives of virology 2014-05, Vol.159 (5), p.1109-1117 |
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| creator | Delfino, Cecilia María Gentile, Emiliano Alberto Castillo, Amalia Inés Cuestas, María Luján Pataccini, Gabriela Cánepa, Camila Malan, Richard Blejer, Jorgelina Berini, Carolina Eirin, María Emilia Pedrozo, Williams Oubiña, José Raúl Biglione, Mirna Marcela Mathet, Verónica Lidia |
| description | In Argentina, current procedures to ensure the safety of the blood supply for transfusion include the serologic detection of specific blood-borne infections. The aim of this study was to evaluate the prevalence and the genetic diversity of hepatitis B virus (HBV) and hepatitis D virus (HDV) in blood donor populations from two distantly located Argentine regions. Data from 56,983 blood donations from the Favaloro Foundation, in the city of Buenos Aires (Central Region), and the Central Blood Bank of Misiones Province (Northeast Region) were analyzed. Samples that were reactive for HBsAg were analyzed for HBV-DNA characterization and HDV serological and molecular analysis. The HBV prevalence was 0.12 % for HBsAg and 1.68 % for anti-HBc antibodies in Buenos Aires, and 0.73 % and 8.55 %, respectively, in Misiones. Seventy-seven HBsAg-reactive samples were analyzed by polymerase chain reaction for HBV-DNA. Subgenotypes A2, B2, C2, F1b and F4 (Buenos Aires) and F1b and D3 (Misiones) were detected. Several mutations within the major hydrophilic region of HBsAg, the reverse transcriptase, the basal core promoter, and the precore/core were detected. HDV genotype 1 was identified in Buenos Aires. This study confirms the circulation of several HBV subgenotypes, as well as known and newly identified variants, and the presence of HDV1 in this population. A thorough investigation has to be carried out to evaluate the clinical importance of some of the documented mutations as well as those detected in the HDV1 case. |
| doi_str_mv | 10.1007/s00705-013-1917-y |
| format | Article |
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The aim of this study was to evaluate the prevalence and the genetic diversity of hepatitis B virus (HBV) and hepatitis D virus (HDV) in blood donor populations from two distantly located Argentine regions. Data from 56,983 blood donations from the Favaloro Foundation, in the city of Buenos Aires (Central Region), and the Central Blood Bank of Misiones Province (Northeast Region) were analyzed. Samples that were reactive for HBsAg were analyzed for HBV-DNA characterization and HDV serological and molecular analysis. The HBV prevalence was 0.12 % for HBsAg and 1.68 % for anti-HBc antibodies in Buenos Aires, and 0.73 % and 8.55 %, respectively, in Misiones. Seventy-seven HBsAg-reactive samples were analyzed by polymerase chain reaction for HBV-DNA. Subgenotypes A2, B2, C2, F1b and F4 (Buenos Aires) and F1b and D3 (Misiones) were detected. Several mutations within the major hydrophilic region of HBsAg, the reverse transcriptase, the basal core promoter, and the precore/core were detected. HDV genotype 1 was identified in Buenos Aires. This study confirms the circulation of several HBV subgenotypes, as well as known and newly identified variants, and the presence of HDV1 in this population. A thorough investigation has to be carried out to evaluate the clinical importance of some of the documented mutations as well as those detected in the HDV1 case.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/s00705-013-1917-y</identifier><identifier>PMID: 24306325</identifier><language>eng</language><publisher>Vienna: Springer-Verlag</publisher><subject>Antibodies ; Argentina - epidemiology ; Biomedical and Life Sciences ; Biomedicine ; blood ; Blood & organ donations ; Blood banks ; Blood Donors ; Cloning ; Cloning, Molecular ; DNA, Viral - genetics ; Gene Expression Regulation, Enzymologic - physiology ; Gene Expression Regulation, Viral - physiology ; Genetic diversity ; genetic variation ; genotype ; Genotype & phenotype ; Hepatitis B ; Hepatitis B - blood ; Hepatitis B - epidemiology ; Hepatitis B - virology ; Hepatitis B Surface Antigens - blood ; Hepatitis B Surface Antigens - genetics ; Hepatitis B virus ; Hepatitis B virus - isolation & purification ; Hepatitis D - blood ; Hepatitis D - epidemiology ; Hepatitis D - virology ; Hepatitis D virus ; Hepatitis delta virus ; Hepatitis Delta Virus - isolation & purification ; Humans ; Infections ; Infectious Diseases ; Liver cancer ; Medical Microbiology ; mutants ; Mutation ; Original Article ; Phylogeny ; Polymerase chain reaction ; Polymorphism, Restriction Fragment Length ; RNA-directed DNA polymerase ; RNA-Directed DNA Polymerase - genetics ; RNA-Directed DNA Polymerase - metabolism ; Serology ; Virology ; Viruses</subject><ispartof>Archives of virology, 2014-05, Vol.159 (5), p.1109-1117</ispartof><rights>Springer-Verlag Wien 2013</rights><rights>Springer-Verlag Wien 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-4297a6714a0abd85185f681de392d502297e56795b4640e8c24b6cd1a6815b983</citedby><cites>FETCH-LOGICAL-c429t-4297a6714a0abd85185f681de392d502297e56795b4640e8c24b6cd1a6815b983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00705-013-1917-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00705-013-1917-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24306325$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Delfino, Cecilia María</creatorcontrib><creatorcontrib>Gentile, Emiliano Alberto</creatorcontrib><creatorcontrib>Castillo, Amalia Inés</creatorcontrib><creatorcontrib>Cuestas, María Luján</creatorcontrib><creatorcontrib>Pataccini, Gabriela</creatorcontrib><creatorcontrib>Cánepa, Camila</creatorcontrib><creatorcontrib>Malan, Richard</creatorcontrib><creatorcontrib>Blejer, Jorgelina</creatorcontrib><creatorcontrib>Berini, Carolina</creatorcontrib><creatorcontrib>Eirin, María Emilia</creatorcontrib><creatorcontrib>Pedrozo, Williams</creatorcontrib><creatorcontrib>Oubiña, José Raúl</creatorcontrib><creatorcontrib>Biglione, Mirna Marcela</creatorcontrib><creatorcontrib>Mathet, Verónica Lidia</creatorcontrib><title>Hepatitis B virus and hepatitis D virus in blood donors from Argentina: circulation of HBsAg and reverse transcriptase mutants</title><title>Archives of virology</title><addtitle>Arch Virol</addtitle><addtitle>Arch Virol</addtitle><description>In Argentina, current procedures to ensure the safety of the blood supply for transfusion include the serologic detection of specific blood-borne infections. The aim of this study was to evaluate the prevalence and the genetic diversity of hepatitis B virus (HBV) and hepatitis D virus (HDV) in blood donor populations from two distantly located Argentine regions. Data from 56,983 blood donations from the Favaloro Foundation, in the city of Buenos Aires (Central Region), and the Central Blood Bank of Misiones Province (Northeast Region) were analyzed. Samples that were reactive for HBsAg were analyzed for HBV-DNA characterization and HDV serological and molecular analysis. The HBV prevalence was 0.12 % for HBsAg and 1.68 % for anti-HBc antibodies in Buenos Aires, and 0.73 % and 8.55 %, respectively, in Misiones. Seventy-seven HBsAg-reactive samples were analyzed by polymerase chain reaction for HBV-DNA. Subgenotypes A2, B2, C2, F1b and F4 (Buenos Aires) and F1b and D3 (Misiones) were detected. Several mutations within the major hydrophilic region of HBsAg, the reverse transcriptase, the basal core promoter, and the precore/core were detected. HDV genotype 1 was identified in Buenos Aires. This study confirms the circulation of several HBV subgenotypes, as well as known and newly identified variants, and the presence of HDV1 in this population. A thorough investigation has to be carried out to evaluate the clinical importance of some of the documented mutations as well as those detected in the HDV1 case.</description><subject>Antibodies</subject><subject>Argentina - epidemiology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>blood</subject><subject>Blood & organ donations</subject><subject>Blood banks</subject><subject>Blood Donors</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>DNA, Viral - genetics</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>Gene Expression Regulation, Viral - physiology</subject><subject>Genetic diversity</subject><subject>genetic variation</subject><subject>genotype</subject><subject>Genotype & phenotype</subject><subject>Hepatitis B</subject><subject>Hepatitis B - blood</subject><subject>Hepatitis B - epidemiology</subject><subject>Hepatitis B - virology</subject><subject>Hepatitis B Surface Antigens - blood</subject><subject>Hepatitis B Surface Antigens - genetics</subject><subject>Hepatitis B virus</subject><subject>Hepatitis B virus - isolation & purification</subject><subject>Hepatitis D - blood</subject><subject>Hepatitis D - epidemiology</subject><subject>Hepatitis D - virology</subject><subject>Hepatitis D virus</subject><subject>Hepatitis delta virus</subject><subject>Hepatitis Delta Virus - isolation & purification</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious Diseases</subject><subject>Liver cancer</subject><subject>Medical Microbiology</subject><subject>mutants</subject><subject>Mutation</subject><subject>Original Article</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>RNA-directed DNA polymerase</subject><subject>RNA-Directed DNA Polymerase - genetics</subject><subject>RNA-Directed DNA Polymerase - metabolism</subject><subject>Serology</subject><subject>Virology</subject><subject>Viruses</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkktv1DAUhS0EokPhB7ABS92wCb1-xQ67aXlMpUosoGvLSZzBVWIPtlNpNvz2eshQoS4QG1u-9zvnyj5G6DWB9wRAnqeygKiAsIo0RFb7J2hFOKOVko16ilbAgFeqBnWCXqR0C1AKTDxHJ5QzqBkVK_RrY3cmu-wSvsB3Ls4JG9_jHw_Vj8eq87gdQ-hxH3yICQ8xTHgdt9Zn580H3LnYzWMRBY_DgDcXab39bRXtnY3J4hyNT110u2zKaZqz8Tm9RM8GMyb76rifopvPn75fbqrrr1-uLtfXVcdpk6uySFNLwg2YtleCKDHUivSWNbQXQEvbilo2ouU1B6s6ytu664kpkGgbxU7Ru8V3F8PP2aasJ5c6O47G2zAnTQTlnEho5P-g5bHLSCjo2SP0NszRl4scKFA1b-iBIgvVxZBStIPeRTeZuNcE9CFHveSoS476kKPeF82bo_PcTrZ_UPwJrgB0AVJp-a2Nf43-h-vbRTSYoM02uqRvvlEgvPwMJYUCdg8qUrDV</recordid><startdate>20140501</startdate><enddate>20140501</enddate><creator>Delfino, Cecilia María</creator><creator>Gentile, Emiliano Alberto</creator><creator>Castillo, Amalia Inés</creator><creator>Cuestas, María Luján</creator><creator>Pataccini, Gabriela</creator><creator>Cánepa, Camila</creator><creator>Malan, Richard</creator><creator>Blejer, Jorgelina</creator><creator>Berini, Carolina</creator><creator>Eirin, María Emilia</creator><creator>Pedrozo, Williams</creator><creator>Oubiña, José Raúl</creator><creator>Biglione, Mirna Marcela</creator><creator>Mathet, Verónica Lidia</creator><general>Springer-Verlag</general><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20140501</creationdate><title>Hepatitis B virus and hepatitis D virus in blood donors from Argentina: circulation of HBsAg and reverse transcriptase mutants</title><author>Delfino, Cecilia María ; Gentile, Emiliano Alberto ; Castillo, Amalia Inés ; Cuestas, María Luján ; Pataccini, Gabriela ; Cánepa, Camila ; Malan, Richard ; Blejer, Jorgelina ; Berini, Carolina ; Eirin, María Emilia ; Pedrozo, Williams ; Oubiña, José Raúl ; Biglione, Mirna Marcela ; Mathet, Verónica Lidia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-4297a6714a0abd85185f681de392d502297e56795b4640e8c24b6cd1a6815b983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Antibodies</topic><topic>Argentina - epidemiology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>blood</topic><topic>Blood & organ donations</topic><topic>Blood banks</topic><topic>Blood Donors</topic><topic>Cloning</topic><topic>Cloning, Molecular</topic><topic>DNA, Viral - genetics</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>Gene Expression Regulation, Viral - physiology</topic><topic>Genetic diversity</topic><topic>genetic variation</topic><topic>genotype</topic><topic>Genotype & phenotype</topic><topic>Hepatitis B</topic><topic>Hepatitis B - blood</topic><topic>Hepatitis B - epidemiology</topic><topic>Hepatitis B - virology</topic><topic>Hepatitis B Surface Antigens - blood</topic><topic>Hepatitis B Surface Antigens - genetics</topic><topic>Hepatitis B virus</topic><topic>Hepatitis B virus - isolation & purification</topic><topic>Hepatitis D - blood</topic><topic>Hepatitis D - epidemiology</topic><topic>Hepatitis D - virology</topic><topic>Hepatitis D virus</topic><topic>Hepatitis delta virus</topic><topic>Hepatitis Delta Virus - 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Academic</collection><jtitle>Archives of virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Delfino, Cecilia María</au><au>Gentile, Emiliano Alberto</au><au>Castillo, Amalia Inés</au><au>Cuestas, María Luján</au><au>Pataccini, Gabriela</au><au>Cánepa, Camila</au><au>Malan, Richard</au><au>Blejer, Jorgelina</au><au>Berini, Carolina</au><au>Eirin, María Emilia</au><au>Pedrozo, Williams</au><au>Oubiña, José Raúl</au><au>Biglione, Mirna Marcela</au><au>Mathet, Verónica Lidia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hepatitis B virus and hepatitis D virus in blood donors from Argentina: circulation of HBsAg and reverse transcriptase mutants</atitle><jtitle>Archives of virology</jtitle><stitle>Arch Virol</stitle><addtitle>Arch Virol</addtitle><date>2014-05-01</date><risdate>2014</risdate><volume>159</volume><issue>5</issue><spage>1109</spage><epage>1117</epage><pages>1109-1117</pages><issn>0304-8608</issn><eissn>1432-8798</eissn><abstract>In Argentina, current procedures to ensure the safety of the blood supply for transfusion include the serologic detection of specific blood-borne infections. The aim of this study was to evaluate the prevalence and the genetic diversity of hepatitis B virus (HBV) and hepatitis D virus (HDV) in blood donor populations from two distantly located Argentine regions. Data from 56,983 blood donations from the Favaloro Foundation, in the city of Buenos Aires (Central Region), and the Central Blood Bank of Misiones Province (Northeast Region) were analyzed. Samples that were reactive for HBsAg were analyzed for HBV-DNA characterization and HDV serological and molecular analysis. The HBV prevalence was 0.12 % for HBsAg and 1.68 % for anti-HBc antibodies in Buenos Aires, and 0.73 % and 8.55 %, respectively, in Misiones. Seventy-seven HBsAg-reactive samples were analyzed by polymerase chain reaction for HBV-DNA. Subgenotypes A2, B2, C2, F1b and F4 (Buenos Aires) and F1b and D3 (Misiones) were detected. Several mutations within the major hydrophilic region of HBsAg, the reverse transcriptase, the basal core promoter, and the precore/core were detected. HDV genotype 1 was identified in Buenos Aires. This study confirms the circulation of several HBV subgenotypes, as well as known and newly identified variants, and the presence of HDV1 in this population. A thorough investigation has to be carried out to evaluate the clinical importance of some of the documented mutations as well as those detected in the HDV1 case.</abstract><cop>Vienna</cop><pub>Springer-Verlag</pub><pmid>24306325</pmid><doi>10.1007/s00705-013-1917-y</doi><tpages>9</tpages></addata></record> |
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| ispartof | Archives of virology, 2014-05, Vol.159 (5), p.1109-1117 |
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| subjects | Antibodies Argentina - epidemiology Biomedical and Life Sciences Biomedicine blood Blood & organ donations Blood banks Blood Donors Cloning Cloning, Molecular DNA, Viral - genetics Gene Expression Regulation, Enzymologic - physiology Gene Expression Regulation, Viral - physiology Genetic diversity genetic variation genotype Genotype & phenotype Hepatitis B Hepatitis B - blood Hepatitis B - epidemiology Hepatitis B - virology Hepatitis B Surface Antigens - blood Hepatitis B Surface Antigens - genetics Hepatitis B virus Hepatitis B virus - isolation & purification Hepatitis D - blood Hepatitis D - epidemiology Hepatitis D - virology Hepatitis D virus Hepatitis delta virus Hepatitis Delta Virus - isolation & purification Humans Infections Infectious Diseases Liver cancer Medical Microbiology mutants Mutation Original Article Phylogeny Polymerase chain reaction Polymorphism, Restriction Fragment Length RNA-directed DNA polymerase RNA-Directed DNA Polymerase - genetics RNA-Directed DNA Polymerase - metabolism Serology Virology Viruses |
| title | Hepatitis B virus and hepatitis D virus in blood donors from Argentina: circulation of HBsAg and reverse transcriptase mutants |
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