Modulation effect of Smilax glabra flavonoids on ryanodine receptor mediated intracellular Ca2+ release in cardiomyoblast cells
Smilax glabra rhizome, a plant material from Liliaceae family, is a widely used traditional Chinese medicine for anti-cardiac hypertrophy treatment. We have previously found that Smilax glabra flavonoids (SGF) exerted such anti-cardiac hypertrophy activity. However, the mechanism of this activity of...
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| Veröffentlicht in: | Journal of ethnopharmacology 2013-10, Vol.150 (1), p.389-392 |
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| creator | Shou, Qiyang Pan, Shuizhen Tu, Jue Jiang, Jianping Ling, Yun Cai, Yueqin Chen, Minli Wang, Dejun |
| description | Smilax glabra rhizome, a plant material from Liliaceae family, is a widely used traditional Chinese medicine for anti-cardiac hypertrophy treatment. We have previously found that Smilax glabra flavonoids (SGF) exerted such anti-cardiac hypertrophy activity. However, the mechanism of this activity of SGF has not been clarified yet.
This study was aimed to investigate the inhibitory role of SGF on intracellular Ca2+ release in rat cardiomyoblast cells (H9C2). Intracellular Ca2+ release was determined by Ca2+ indicator fluorescence (fluo 4-AM) in H9C2 cell line.
SGF at concentrations of 0.25, 0.5, 1.0mg/ml significantly inhibited the phenylephrine or angiotensin II induced intracellular Ca2+ release in a dose-dependent manner. Furthermore, SGF could also inhibit ryanodine receptor (RyR) agonist caffeine induced Ca2+ release and phenylephrine (PE)-induced Ca2+ release under the condition in which inositol trisphosphate (IP3) receptors were blocked with 2-Aminoethoxydiphenyl borate (2-APB). Nevertheless, SGF had no impact on PE-induced Ca2+ release under the condition in which RyRs were blocked with tetracaine.
Our results suggest that the protective effects of SGF are mediated via targeting inhibition of RyR mediated intracellular Ca2+ release. |
| doi_str_mv | 10.1016/j.jep.2013.08.009 |
| format | Article |
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This study was aimed to investigate the inhibitory role of SGF on intracellular Ca2+ release in rat cardiomyoblast cells (H9C2). Intracellular Ca2+ release was determined by Ca2+ indicator fluorescence (fluo 4-AM) in H9C2 cell line.
SGF at concentrations of 0.25, 0.5, 1.0mg/ml significantly inhibited the phenylephrine or angiotensin II induced intracellular Ca2+ release in a dose-dependent manner. Furthermore, SGF could also inhibit ryanodine receptor (RyR) agonist caffeine induced Ca2+ release and phenylephrine (PE)-induced Ca2+ release under the condition in which inositol trisphosphate (IP3) receptors were blocked with 2-Aminoethoxydiphenyl borate (2-APB). Nevertheless, SGF had no impact on PE-induced Ca2+ release under the condition in which RyRs were blocked with tetracaine.
Our results suggest that the protective effects of SGF are mediated via targeting inhibition of RyR mediated intracellular Ca2+ release.</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2013.08.009</identifier><identifier>PMID: 23954280</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>agonists ; angiotensin II ; Animals ; caffeine ; calcium ; Calcium Signaling - drug effects ; Cell Line ; flavonoids ; Flavonoids - pharmacology ; fluorescence ; hypertrophy ; Inositol 1,4,5-trisphosphate receptors ; Intracellular Ca2+ release ; Liliaceae ; myo-inositol ; Myoblasts, Cardiac - drug effects ; Myoblasts, Cardiac - metabolism ; Oriental traditional medicine ; phenylephrine ; Rats ; receptors ; Ryanodine Receptor Calcium Release Channel - metabolism ; Ryanodine receptors ; Smilax ; Smilax glabra flavonoids</subject><ispartof>Journal of ethnopharmacology, 2013-10, Vol.150 (1), p.389-392</ispartof><rights>2013 Elsevier Ireland Ltd</rights><rights>2013 Published by Elsevier Ireland Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c340t-fba9f3ef5623ac91e7f0c688b64ed089b296b956ba0d436598294407b1f8b793</citedby><cites>FETCH-LOGICAL-c340t-fba9f3ef5623ac91e7f0c688b64ed089b296b956ba0d436598294407b1f8b793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378874113005515$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23954280$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shou, Qiyang</creatorcontrib><creatorcontrib>Pan, Shuizhen</creatorcontrib><creatorcontrib>Tu, Jue</creatorcontrib><creatorcontrib>Jiang, Jianping</creatorcontrib><creatorcontrib>Ling, Yun</creatorcontrib><creatorcontrib>Cai, Yueqin</creatorcontrib><creatorcontrib>Chen, Minli</creatorcontrib><creatorcontrib>Wang, Dejun</creatorcontrib><title>Modulation effect of Smilax glabra flavonoids on ryanodine receptor mediated intracellular Ca2+ release in cardiomyoblast cells</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>Smilax glabra rhizome, a plant material from Liliaceae family, is a widely used traditional Chinese medicine for anti-cardiac hypertrophy treatment. We have previously found that Smilax glabra flavonoids (SGF) exerted such anti-cardiac hypertrophy activity. However, the mechanism of this activity of SGF has not been clarified yet.
This study was aimed to investigate the inhibitory role of SGF on intracellular Ca2+ release in rat cardiomyoblast cells (H9C2). Intracellular Ca2+ release was determined by Ca2+ indicator fluorescence (fluo 4-AM) in H9C2 cell line.
SGF at concentrations of 0.25, 0.5, 1.0mg/ml significantly inhibited the phenylephrine or angiotensin II induced intracellular Ca2+ release in a dose-dependent manner. Furthermore, SGF could also inhibit ryanodine receptor (RyR) agonist caffeine induced Ca2+ release and phenylephrine (PE)-induced Ca2+ release under the condition in which inositol trisphosphate (IP3) receptors were blocked with 2-Aminoethoxydiphenyl borate (2-APB). Nevertheless, SGF had no impact on PE-induced Ca2+ release under the condition in which RyRs were blocked with tetracaine.
Our results suggest that the protective effects of SGF are mediated via targeting inhibition of RyR mediated intracellular Ca2+ release.</description><subject>agonists</subject><subject>angiotensin II</subject><subject>Animals</subject><subject>caffeine</subject><subject>calcium</subject><subject>Calcium Signaling - drug effects</subject><subject>Cell Line</subject><subject>flavonoids</subject><subject>Flavonoids - pharmacology</subject><subject>fluorescence</subject><subject>hypertrophy</subject><subject>Inositol 1,4,5-trisphosphate receptors</subject><subject>Intracellular Ca2+ release</subject><subject>Liliaceae</subject><subject>myo-inositol</subject><subject>Myoblasts, Cardiac - drug effects</subject><subject>Myoblasts, Cardiac - metabolism</subject><subject>Oriental traditional medicine</subject><subject>phenylephrine</subject><subject>Rats</subject><subject>receptors</subject><subject>Ryanodine Receptor Calcium Release Channel - metabolism</subject><subject>Ryanodine receptors</subject><subject>Smilax</subject><subject>Smilax glabra flavonoids</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EokvhB3ABH5FQgr-S2OJUrfiSiji0nK2xM668SuLFzlbsib-OV1s4wmkO88yr0fsQ8pKzljPev9u1O9y3gnHZMt0yZh6RDdeDaIZukI_JhslBN3pQ_II8K2XHGBu4Yk_JhZCmU0KzDfn1NY2HCdaYFoohoF9pCvRmjhP8pHcTuAw0THCflhTHQiuVj7CkMS5IM3rcrynTGccIK440LmsGj9NUIzPdgnhboQmhYF1RD3mMaT4mN0FZ6Ykrz8mTAFPBFw_zktx-_HC7_dxcf_v0ZXt13Xip2NoEByZIDF0vJHjDcQjM91q7XuHItHHC9M50vQM2Ktl3RgujFBscD9oNRl6SN-fYfU4_DlhWO8dyegAWTIdieSeUqt0Y8X9UKSmNUUJWlJ9Rn1MpGYPd5zhDPlrO7MmQ3dlqyJ4MWaZtNVRvXj3EH1zt7e_FHyUVeH0GAiQLdzkW-_2mJqiqT-ledZV4fyawFnYfMdviIy6-WqhKVjum-I8HfgO646uf</recordid><startdate>20131028</startdate><enddate>20131028</enddate><creator>Shou, Qiyang</creator><creator>Pan, Shuizhen</creator><creator>Tu, Jue</creator><creator>Jiang, Jianping</creator><creator>Ling, Yun</creator><creator>Cai, Yueqin</creator><creator>Chen, Minli</creator><creator>Wang, Dejun</creator><general>Elsevier B.V</general><general>Elsevier Ireland Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QP</scope></search><sort><creationdate>20131028</creationdate><title>Modulation effect of Smilax glabra flavonoids on ryanodine receptor mediated intracellular Ca2+ release in cardiomyoblast cells</title><author>Shou, Qiyang ; Pan, Shuizhen ; Tu, Jue ; Jiang, Jianping ; Ling, Yun ; Cai, Yueqin ; Chen, Minli ; Wang, Dejun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c340t-fba9f3ef5623ac91e7f0c688b64ed089b296b956ba0d436598294407b1f8b793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>agonists</topic><topic>angiotensin II</topic><topic>Animals</topic><topic>caffeine</topic><topic>calcium</topic><topic>Calcium Signaling - drug effects</topic><topic>Cell Line</topic><topic>flavonoids</topic><topic>Flavonoids - pharmacology</topic><topic>fluorescence</topic><topic>hypertrophy</topic><topic>Inositol 1,4,5-trisphosphate receptors</topic><topic>Intracellular Ca2+ release</topic><topic>Liliaceae</topic><topic>myo-inositol</topic><topic>Myoblasts, Cardiac - drug effects</topic><topic>Myoblasts, Cardiac - metabolism</topic><topic>Oriental traditional medicine</topic><topic>phenylephrine</topic><topic>Rats</topic><topic>receptors</topic><topic>Ryanodine Receptor Calcium Release Channel - metabolism</topic><topic>Ryanodine receptors</topic><topic>Smilax</topic><topic>Smilax glabra flavonoids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shou, Qiyang</creatorcontrib><creatorcontrib>Pan, Shuizhen</creatorcontrib><creatorcontrib>Tu, Jue</creatorcontrib><creatorcontrib>Jiang, Jianping</creatorcontrib><creatorcontrib>Ling, Yun</creatorcontrib><creatorcontrib>Cai, Yueqin</creatorcontrib><creatorcontrib>Chen, Minli</creatorcontrib><creatorcontrib>Wang, Dejun</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Calcium & Calcified Tissue Abstracts</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shou, Qiyang</au><au>Pan, Shuizhen</au><au>Tu, Jue</au><au>Jiang, Jianping</au><au>Ling, Yun</au><au>Cai, Yueqin</au><au>Chen, Minli</au><au>Wang, Dejun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation effect of Smilax glabra flavonoids on ryanodine receptor mediated intracellular Ca2+ release in cardiomyoblast cells</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2013-10-28</date><risdate>2013</risdate><volume>150</volume><issue>1</issue><spage>389</spage><epage>392</epage><pages>389-392</pages><issn>0378-8741</issn><eissn>1872-7573</eissn><abstract>Smilax glabra rhizome, a plant material from Liliaceae family, is a widely used traditional Chinese medicine for anti-cardiac hypertrophy treatment. We have previously found that Smilax glabra flavonoids (SGF) exerted such anti-cardiac hypertrophy activity. However, the mechanism of this activity of SGF has not been clarified yet.
This study was aimed to investigate the inhibitory role of SGF on intracellular Ca2+ release in rat cardiomyoblast cells (H9C2). Intracellular Ca2+ release was determined by Ca2+ indicator fluorescence (fluo 4-AM) in H9C2 cell line.
SGF at concentrations of 0.25, 0.5, 1.0mg/ml significantly inhibited the phenylephrine or angiotensin II induced intracellular Ca2+ release in a dose-dependent manner. Furthermore, SGF could also inhibit ryanodine receptor (RyR) agonist caffeine induced Ca2+ release and phenylephrine (PE)-induced Ca2+ release under the condition in which inositol trisphosphate (IP3) receptors were blocked with 2-Aminoethoxydiphenyl borate (2-APB). Nevertheless, SGF had no impact on PE-induced Ca2+ release under the condition in which RyRs were blocked with tetracaine.
Our results suggest that the protective effects of SGF are mediated via targeting inhibition of RyR mediated intracellular Ca2+ release.</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>23954280</pmid><doi>10.1016/j.jep.2013.08.009</doi><tpages>4</tpages></addata></record> |
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| subjects | agonists angiotensin II Animals caffeine calcium Calcium Signaling - drug effects Cell Line flavonoids Flavonoids - pharmacology fluorescence hypertrophy Inositol 1,4,5-trisphosphate receptors Intracellular Ca2+ release Liliaceae myo-inositol Myoblasts, Cardiac - drug effects Myoblasts, Cardiac - metabolism Oriental traditional medicine phenylephrine Rats receptors Ryanodine Receptor Calcium Release Channel - metabolism Ryanodine receptors Smilax Smilax glabra flavonoids |
| title | Modulation effect of Smilax glabra flavonoids on ryanodine receptor mediated intracellular Ca2+ release in cardiomyoblast cells |
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