On-column refolding of bone morphogenetic protein-2 using cation exchange resin

•A novel BMP-2 dimer refolding procedure using weak cation exchange resin.•Weakly bound monomer dissociates from resin but refolded dimer rebinds strongly.•Simultaneous refolding and purification using on-column ion exchange chromatography.•Potential applications on refolding of other dimer proteins...

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Veröffentlicht in:	Protein expression and purification 2013-08, Vol.90 (2), p.135-140
Hauptverfasser:	Rane, Anuja M., Jonnalagadda, Sriramakamal, Li, Zhiyu
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline Phosphatase - biosynthesis Animals Bone Morphogenetic Protein 2 - chemistry Bone Morphogenetic Protein 2 - pharmacology Bone morphogenetic protein-2 Cation exchange chromatography Cation Exchange Resins - chemistry Cell Line Enzyme Induction Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Humans Inclusion body Mice On-column refolding Osteoblasts - drug effects Osteoblasts - enzymology Protein dimer Protein Folding Protein Multimerization Recombinant Proteins - chemistry Recombinant Proteins - pharmacology
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