Coaxially Electrospun Scaffolds Based on Hydroxyl-Functionalized Poly(ε-caprolactone) and Loaded with VEGF for Tissue Engineering Applications
The aim of this study was to fabricate nanofibrous scaffolds based on blends of a hydroxyl functionalized polyester (poly(hydroxymethylglycolide-co-ε-caprolactone), pHMGCL) and poly(ε-caprolactone) (PCL), loaded with bovine serum albumin (BSA) as a protein stabilizer and vascular endothelial growth...
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| Veröffentlicht in: | Biomacromolecules 2012-11, Vol.13 (11), p.3650-3660 |
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| creator | Seyednejad, Hajar Ji, Wei Yang, Fang van Nostrum, Cornelus F Vermonden, Tina van den Beucken, Jeroen J.J.P Dhert, Wouter J.A Hennink, Wim E Jansen, John A |
| description | The aim of this study was to fabricate nanofibrous scaffolds based on blends of a hydroxyl functionalized polyester (poly(hydroxymethylglycolide-co-ε-caprolactone), pHMGCL) and poly(ε-caprolactone) (PCL), loaded with bovine serum albumin (BSA) as a protein stabilizer and vascular endothelial growth factor (VEGF) as a potent angiogenic factor by means of a coaxial electrospinning technique. The scaffolds were characterized by scanning electron microscopy (SEM), fluorescence microscopy (FM), and differential scanning calorimetry (DSC). The scaffolds displayed a uniform fibrous structure with a fiber diameter around 700 nm. The release of BSA from the core of the fibers was studied by high performance liquid chromatography (HPLC), and it was shown that the coaxial scaffolds composed of blends of pHMGCL and PCL exhibited faster release than the comparative PCL scaffolds. VEGF was also incorporated in the core of the scaffolds, and the effect of the released protein on the attachment and proliferation of endothelial cells was investigated. It was shown that the incorporated protein preserved its biological activity and resulted in initial higher numbers of adhered cells. Thus, these bioactive scaffolds based on blends of pHMGCL/PCL loaded with VEGF can be considered as a promising candidate for tissue engineering applications. |
| doi_str_mv | 10.1021/bm301101r |
| format | Article |
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The scaffolds were characterized by scanning electron microscopy (SEM), fluorescence microscopy (FM), and differential scanning calorimetry (DSC). The scaffolds displayed a uniform fibrous structure with a fiber diameter around 700 nm. The release of BSA from the core of the fibers was studied by high performance liquid chromatography (HPLC), and it was shown that the coaxial scaffolds composed of blends of pHMGCL and PCL exhibited faster release than the comparative PCL scaffolds. VEGF was also incorporated in the core of the scaffolds, and the effect of the released protein on the attachment and proliferation of endothelial cells was investigated. It was shown that the incorporated protein preserved its biological activity and resulted in initial higher numbers of adhered cells. Thus, these bioactive scaffolds based on blends of pHMGCL/PCL loaded with VEGF can be considered as a promising candidate for tissue engineering applications.</description><identifier>ISSN: 1525-7797</identifier><identifier>EISSN: 1526-4602</identifier><identifier>DOI: 10.1021/bm301101r</identifier><identifier>PMID: 23039047</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Applied sciences ; Biological and medical sciences ; Cell Line ; Exact sciences and technology ; Fibers and threads ; Forms of application and semi-finished materials ; Humans ; Medical sciences ; Polyesters - chemistry ; Polymer industry, paints, wood ; Serum Albumin, Bovine - metabolism ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Technology of polymers ; Technology. Biomaterials. Equipments ; Tissue Engineering - methods ; Tissue Scaffolds ; Vascular Endothelial Growth Factor A - metabolism</subject><ispartof>Biomacromolecules, 2012-11, Vol.13 (11), p.3650-3660</ispartof><rights>Copyright © 2012 American Chemical Society</rights><rights>2014 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a378t-ec511eb8d26bdbd80b6eb6408553ab4035a2d7adc49b924bd64f7e993aa973dd3</citedby><cites>FETCH-LOGICAL-a378t-ec511eb8d26bdbd80b6eb6408553ab4035a2d7adc49b924bd64f7e993aa973dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bm301101r$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bm301101r$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=26603285$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23039047$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Seyednejad, Hajar</creatorcontrib><creatorcontrib>Ji, Wei</creatorcontrib><creatorcontrib>Yang, Fang</creatorcontrib><creatorcontrib>van Nostrum, Cornelus F</creatorcontrib><creatorcontrib>Vermonden, Tina</creatorcontrib><creatorcontrib>van den Beucken, Jeroen J.J.P</creatorcontrib><creatorcontrib>Dhert, Wouter J.A</creatorcontrib><creatorcontrib>Hennink, Wim E</creatorcontrib><creatorcontrib>Jansen, John A</creatorcontrib><title>Coaxially Electrospun Scaffolds Based on Hydroxyl-Functionalized Poly(ε-caprolactone) and Loaded with VEGF for Tissue Engineering Applications</title><title>Biomacromolecules</title><addtitle>Biomacromolecules</addtitle><description>The aim of this study was to fabricate nanofibrous scaffolds based on blends of a hydroxyl functionalized polyester (poly(hydroxymethylglycolide-co-ε-caprolactone), pHMGCL) and poly(ε-caprolactone) (PCL), loaded with bovine serum albumin (BSA) as a protein stabilizer and vascular endothelial growth factor (VEGF) as a potent angiogenic factor by means of a coaxial electrospinning technique. The scaffolds were characterized by scanning electron microscopy (SEM), fluorescence microscopy (FM), and differential scanning calorimetry (DSC). The scaffolds displayed a uniform fibrous structure with a fiber diameter around 700 nm. The release of BSA from the core of the fibers was studied by high performance liquid chromatography (HPLC), and it was shown that the coaxial scaffolds composed of blends of pHMGCL and PCL exhibited faster release than the comparative PCL scaffolds. VEGF was also incorporated in the core of the scaffolds, and the effect of the released protein on the attachment and proliferation of endothelial cells was investigated. It was shown that the incorporated protein preserved its biological activity and resulted in initial higher numbers of adhered cells. Thus, these bioactive scaffolds based on blends of pHMGCL/PCL loaded with VEGF can be considered as a promising candidate for tissue engineering applications.</description><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Exact sciences and technology</subject><subject>Fibers and threads</subject><subject>Forms of application and semi-finished materials</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Polyesters - chemistry</subject><subject>Polymer industry, paints, wood</subject><subject>Serum Albumin, Bovine - metabolism</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Technology of polymers</subject><subject>Technology. Biomaterials. Equipments</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Scaffolds</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><issn>1525-7797</issn><issn>1526-4602</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0ctu1DAUBuAIgWgpLHgB5A1SuwjY8S1ZtqOZFmkkkChso-NLiiuPHexENLwET8Nr8Ey4dGg3SKx8JH86tv6_ql4S_IbghrxVO4oJwSQ9qg4Jb0TNBG4e_5l5LWUnD6pnOV9jjDvK-NPqoKGYdpjJw-rHKsKNA-8XtPZWTynmcQ7oo4ZhiN5kdAbZGhQDulhMijeLrzdz0JOLAbz7Xq4-RL8c__pZaxhT9KCnGOwJgmDQNoIp4JubvqDP6_MNGmJCly7n2aJ1uHLB2uTCFTodR-803O7Mz6snA_hsX-zPo-rTZn25uqi378_frU63NVDZTrXVnBCrWtMIZZRpsRJWCYZbzikohimHxkgwmnWqa5gygg3Sdh0F6CQ1hh5Vx3d7y6e_zjZP_c5lbb2HYOOc-xIdY7jk1_6fEk4kloLgQk_uqC455mSHfkxuB2npCe5vq-rvqyr21X7trHbW3Mu_3RTweg8ga_BDgqBdfnBCYNq0_MGBzv11nFNpJv_jwd8PXKnf</recordid><startdate>20121112</startdate><enddate>20121112</enddate><creator>Seyednejad, Hajar</creator><creator>Ji, Wei</creator><creator>Yang, Fang</creator><creator>van Nostrum, Cornelus F</creator><creator>Vermonden, Tina</creator><creator>van den Beucken, Jeroen J.J.P</creator><creator>Dhert, Wouter J.A</creator><creator>Hennink, Wim E</creator><creator>Jansen, John A</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20121112</creationdate><title>Coaxially Electrospun Scaffolds Based on Hydroxyl-Functionalized Poly(ε-caprolactone) and Loaded with VEGF for Tissue Engineering Applications</title><author>Seyednejad, Hajar ; Ji, Wei ; Yang, Fang ; van Nostrum, Cornelus F ; Vermonden, Tina ; van den Beucken, Jeroen J.J.P ; Dhert, Wouter J.A ; Hennink, Wim E ; Jansen, John A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a378t-ec511eb8d26bdbd80b6eb6408553ab4035a2d7adc49b924bd64f7e993aa973dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Exact sciences and technology</topic><topic>Fibers and threads</topic><topic>Forms of application and semi-finished materials</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Polyesters - chemistry</topic><topic>Polymer industry, paints, wood</topic><topic>Serum Albumin, Bovine - metabolism</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Technology of polymers</topic><topic>Technology. Biomaterials. Equipments</topic><topic>Tissue Engineering - methods</topic><topic>Tissue Scaffolds</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seyednejad, Hajar</creatorcontrib><creatorcontrib>Ji, Wei</creatorcontrib><creatorcontrib>Yang, Fang</creatorcontrib><creatorcontrib>van Nostrum, Cornelus F</creatorcontrib><creatorcontrib>Vermonden, Tina</creatorcontrib><creatorcontrib>van den Beucken, Jeroen J.J.P</creatorcontrib><creatorcontrib>Dhert, Wouter J.A</creatorcontrib><creatorcontrib>Hennink, Wim E</creatorcontrib><creatorcontrib>Jansen, John A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biomacromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seyednejad, Hajar</au><au>Ji, Wei</au><au>Yang, Fang</au><au>van Nostrum, Cornelus F</au><au>Vermonden, Tina</au><au>van den Beucken, Jeroen J.J.P</au><au>Dhert, Wouter J.A</au><au>Hennink, Wim E</au><au>Jansen, John A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Coaxially Electrospun Scaffolds Based on Hydroxyl-Functionalized Poly(ε-caprolactone) and Loaded with VEGF for Tissue Engineering Applications</atitle><jtitle>Biomacromolecules</jtitle><addtitle>Biomacromolecules</addtitle><date>2012-11-12</date><risdate>2012</risdate><volume>13</volume><issue>11</issue><spage>3650</spage><epage>3660</epage><pages>3650-3660</pages><issn>1525-7797</issn><eissn>1526-4602</eissn><abstract>The aim of this study was to fabricate nanofibrous scaffolds based on blends of a hydroxyl functionalized polyester (poly(hydroxymethylglycolide-co-ε-caprolactone), pHMGCL) and poly(ε-caprolactone) (PCL), loaded with bovine serum albumin (BSA) as a protein stabilizer and vascular endothelial growth factor (VEGF) as a potent angiogenic factor by means of a coaxial electrospinning technique. The scaffolds were characterized by scanning electron microscopy (SEM), fluorescence microscopy (FM), and differential scanning calorimetry (DSC). The scaffolds displayed a uniform fibrous structure with a fiber diameter around 700 nm. The release of BSA from the core of the fibers was studied by high performance liquid chromatography (HPLC), and it was shown that the coaxial scaffolds composed of blends of pHMGCL and PCL exhibited faster release than the comparative PCL scaffolds. VEGF was also incorporated in the core of the scaffolds, and the effect of the released protein on the attachment and proliferation of endothelial cells was investigated. It was shown that the incorporated protein preserved its biological activity and resulted in initial higher numbers of adhered cells. Thus, these bioactive scaffolds based on blends of pHMGCL/PCL loaded with VEGF can be considered as a promising candidate for tissue engineering applications.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>23039047</pmid><doi>10.1021/bm301101r</doi><tpages>11</tpages></addata></record> |
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| ispartof | Biomacromolecules, 2012-11, Vol.13 (11), p.3650-3660 |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Applied sciences Biological and medical sciences Cell Line Exact sciences and technology Fibers and threads Forms of application and semi-finished materials Humans Medical sciences Polyesters - chemistry Polymer industry, paints, wood Serum Albumin, Bovine - metabolism Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology of polymers Technology. Biomaterials. Equipments Tissue Engineering - methods Tissue Scaffolds Vascular Endothelial Growth Factor A - metabolism |
| title | Coaxially Electrospun Scaffolds Based on Hydroxyl-Functionalized Poly(ε-caprolactone) and Loaded with VEGF for Tissue Engineering Applications |
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