Obtaining sexual genotypes for breeding in buffel grass
Buffel grass (Cenchrus ciliaris L. syn. Pennisetum ciliare (L.) Link) is a species that is highly tolerant to drought and is used primarily as forage in drier regions throughout the subtropics and tropics. It reproduces mainly by apomixis and the acquisition of obligate sexual genotypes or facultati...
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| Veröffentlicht in: | South African journal of botany 2013-09, Vol.88, p.118-123 |
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| container_title | South African journal of botany |
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| creator | Quiroga, M. Grunberg, K. Ribotta, A. López Colomba, E. Carloni, E. Tommasino, E. Luna, C. Griffa, S. |
| description | Buffel grass (Cenchrus ciliaris L. syn. Pennisetum ciliare (L.) Link) is a species that is highly tolerant to drought and is used primarily as forage in drier regions throughout the subtropics and tropics. It reproduces mainly by apomixis and the acquisition of obligate sexual genotypes or facultative apomicts with high levels of sexuality is required for performing crosses and plant improvement. The aim of this study was to obtain sexual genotypes from controlled crosses using obligate apomictic cultivars and a sexual line. Twelve putative hybrid F1 plants were selected morphologically and two of them were identified as sexual genotypes by PCR using specific primers for reproductive mechanism. Cytoembryological analysis showed 65.5 and 71.3% meiotic embryo sacs in these plants and their hybrid nature was corroborated by AFLP. Both highly sexual genotypes could be used as female parents in crosses for obtaining improved cultivars of buffel grass.
•Putative hybrids were obtained through controlled crosses.•Off-type plants were selected based on morpho-agronomic traits.•F1 plants with high sexuality level were identified.•Hybrids were identified with AFLP.•The new, highly sexual selected hybrids can be used as female parents to obtain improved cultivars. |
| doi_str_mv | 10.1016/j.sajb.2013.04.016 |
| format | Article |
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•Putative hybrids were obtained through controlled crosses.•Off-type plants were selected based on morpho-agronomic traits.•F1 plants with high sexuality level were identified.•Hybrids were identified with AFLP.•The new, highly sexual selected hybrids can be used as female parents to obtain improved cultivars.</description><identifier>ISSN: 0254-6299</identifier><identifier>EISSN: 1727-9321</identifier><identifier>DOI: 10.1016/j.sajb.2013.04.016</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>amplified fragment length polymorphism ; apomixis ; Cenchrus ciliaris ; crossing ; cultivars ; drought tolerance ; Facultative apomixis ; forage ; Forage breeding ; genotype ; Hibridization ; hybrids ; parents ; subtropics ; tropics</subject><ispartof>South African journal of botany, 2013-09, Vol.88, p.118-123</ispartof><rights>2013 South African Association of Botanists</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-4e0f0360e9ab5192a15a94b06dc3ee98727610e4908fe157a332aa00e5fd5bd73</citedby><cites>FETCH-LOGICAL-c401t-4e0f0360e9ab5192a15a94b06dc3ee98727610e4908fe157a332aa00e5fd5bd73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0254629913002986$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Quiroga, M.</creatorcontrib><creatorcontrib>Grunberg, K.</creatorcontrib><creatorcontrib>Ribotta, A.</creatorcontrib><creatorcontrib>López Colomba, E.</creatorcontrib><creatorcontrib>Carloni, E.</creatorcontrib><creatorcontrib>Tommasino, E.</creatorcontrib><creatorcontrib>Luna, C.</creatorcontrib><creatorcontrib>Griffa, S.</creatorcontrib><title>Obtaining sexual genotypes for breeding in buffel grass</title><title>South African journal of botany</title><description>Buffel grass (Cenchrus ciliaris L. syn. Pennisetum ciliare (L.) Link) is a species that is highly tolerant to drought and is used primarily as forage in drier regions throughout the subtropics and tropics. It reproduces mainly by apomixis and the acquisition of obligate sexual genotypes or facultative apomicts with high levels of sexuality is required for performing crosses and plant improvement. The aim of this study was to obtain sexual genotypes from controlled crosses using obligate apomictic cultivars and a sexual line. Twelve putative hybrid F1 plants were selected morphologically and two of them were identified as sexual genotypes by PCR using specific primers for reproductive mechanism. Cytoembryological analysis showed 65.5 and 71.3% meiotic embryo sacs in these plants and their hybrid nature was corroborated by AFLP. Both highly sexual genotypes could be used as female parents in crosses for obtaining improved cultivars of buffel grass.
•Putative hybrids were obtained through controlled crosses.•Off-type plants were selected based on morpho-agronomic traits.•F1 plants with high sexuality level were identified.•Hybrids were identified with AFLP.•The new, highly sexual selected hybrids can be used as female parents to obtain improved cultivars.</description><subject>amplified fragment length polymorphism</subject><subject>apomixis</subject><subject>Cenchrus ciliaris</subject><subject>crossing</subject><subject>cultivars</subject><subject>drought tolerance</subject><subject>Facultative apomixis</subject><subject>forage</subject><subject>Forage breeding</subject><subject>genotype</subject><subject>Hibridization</subject><subject>hybrids</subject><subject>parents</subject><subject>subtropics</subject><subject>tropics</subject><issn>0254-6299</issn><issn>1727-9321</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNp9kEtLw0AQgBdRsFb_gBdz9JI4-0q64EWKLyj0oD0vm2S2bEiTupuI_fduiGdPAzPfvD5CbilkFGj-0GTBNGXGgPIMRBZTZ2RBC1akijN6ThbApEhzptQluQqhgQiyFVuQYlsOxnWu2ycBf0bTJnvs-uF0xJDY3ielR6ynquuScrQWI-BNCNfkwpo24M1fXJLdy_Pn-i3dbF_f10-btBJAh1QgWOA5oDKlpIoZKo0SJeR1xRHVKl6YU0ChYGWRysJwzowBQGlrWdYFX5L7ee7R918jhkEfXKiwbU2H_Rg0lUxwJRSXEWUzWvk-BI9WH707GH_SFPRkSTd6sqQnSxqEjqnYdDc3WdNrs_cu6N1HBCRERauCTxc8zgTGN78deh0qh10VtXisBl337r8Fv9sHeJU</recordid><startdate>20130901</startdate><enddate>20130901</enddate><creator>Quiroga, M.</creator><creator>Grunberg, K.</creator><creator>Ribotta, A.</creator><creator>López Colomba, E.</creator><creator>Carloni, E.</creator><creator>Tommasino, E.</creator><creator>Luna, C.</creator><creator>Griffa, S.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>F1W</scope><scope>H98</scope><scope>L.G</scope></search><sort><creationdate>20130901</creationdate><title>Obtaining sexual genotypes for breeding in buffel grass</title><author>Quiroga, M. ; Grunberg, K. ; Ribotta, A. ; López Colomba, E. ; Carloni, E. ; Tommasino, E. ; Luna, C. ; Griffa, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-4e0f0360e9ab5192a15a94b06dc3ee98727610e4908fe157a332aa00e5fd5bd73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>amplified fragment length polymorphism</topic><topic>apomixis</topic><topic>Cenchrus ciliaris</topic><topic>crossing</topic><topic>cultivars</topic><topic>drought tolerance</topic><topic>Facultative apomixis</topic><topic>forage</topic><topic>Forage breeding</topic><topic>genotype</topic><topic>Hibridization</topic><topic>hybrids</topic><topic>parents</topic><topic>subtropics</topic><topic>tropics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Quiroga, M.</creatorcontrib><creatorcontrib>Grunberg, K.</creatorcontrib><creatorcontrib>Ribotta, A.</creatorcontrib><creatorcontrib>López Colomba, E.</creatorcontrib><creatorcontrib>Carloni, E.</creatorcontrib><creatorcontrib>Tommasino, E.</creatorcontrib><creatorcontrib>Luna, C.</creatorcontrib><creatorcontrib>Griffa, S.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>CrossRef</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>South African journal of botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Quiroga, M.</au><au>Grunberg, K.</au><au>Ribotta, A.</au><au>López Colomba, E.</au><au>Carloni, E.</au><au>Tommasino, E.</au><au>Luna, C.</au><au>Griffa, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Obtaining sexual genotypes for breeding in buffel grass</atitle><jtitle>South African journal of botany</jtitle><date>2013-09-01</date><risdate>2013</risdate><volume>88</volume><spage>118</spage><epage>123</epage><pages>118-123</pages><issn>0254-6299</issn><eissn>1727-9321</eissn><abstract>Buffel grass (Cenchrus ciliaris L. syn. Pennisetum ciliare (L.) Link) is a species that is highly tolerant to drought and is used primarily as forage in drier regions throughout the subtropics and tropics. It reproduces mainly by apomixis and the acquisition of obligate sexual genotypes or facultative apomicts with high levels of sexuality is required for performing crosses and plant improvement. The aim of this study was to obtain sexual genotypes from controlled crosses using obligate apomictic cultivars and a sexual line. Twelve putative hybrid F1 plants were selected morphologically and two of them were identified as sexual genotypes by PCR using specific primers for reproductive mechanism. Cytoembryological analysis showed 65.5 and 71.3% meiotic embryo sacs in these plants and their hybrid nature was corroborated by AFLP. Both highly sexual genotypes could be used as female parents in crosses for obtaining improved cultivars of buffel grass.
•Putative hybrids were obtained through controlled crosses.•Off-type plants were selected based on morpho-agronomic traits.•F1 plants with high sexuality level were identified.•Hybrids were identified with AFLP.•The new, highly sexual selected hybrids can be used as female parents to obtain improved cultivars.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.sajb.2013.04.016</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | amplified fragment length polymorphism apomixis Cenchrus ciliaris crossing cultivars drought tolerance Facultative apomixis forage Forage breeding genotype Hibridization hybrids parents subtropics tropics |
| title | Obtaining sexual genotypes for breeding in buffel grass |
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