Enteroaggregative Escherichia coli in diarrheic children in Egypt: molecular characterization and antimicrobial susceptibility
Little information is available regarding the significance of enteroaggregative Escherichia coli (EAEC) in pediatric diarrhea in Egypt. Escherichia coli was isolated from stool samples of 62 diarrheic and 43 non-diarrheic (control) Egyptian children. Samples were screened for genes specific for ente...
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| Veröffentlicht in: | Journal of infection in developing countries 2014-05, Vol.8 (5), p.589-596 |
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| creator | Ali, Mostafa Mohamed M Ahmed, Salwa Fouad Klena, John D Mohamed, Zienat Kamel Moussa, Tarek A A Ghenghesh, Khalifa Sifaw |
| description | Little information is available regarding the significance of enteroaggregative Escherichia coli (EAEC) in pediatric diarrhea in Egypt.
Escherichia coli was isolated from stool samples of 62 diarrheic and 43 non-diarrheic (control) Egyptian children. Samples were screened for genes specific for enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), Shiga toxin-producing E. coli (STEC), and enteroinvasive E. coli (EIEC) using polymerase chain reaction (PCR). Diarrheagenic E. coli were grouped phylogenetically using PCR and tested for their susceptibility to antibiotics using the disk diffusion method. Isolates designated as EAEC were examined for eight virulence factors (VFs) using PCR.
EAEC was detected in 19 (30.7%) and 4 (9.3%), EPEC in 2 (3.2%) and 1 (2.3%), and ETEC in 2 (3.2%) and 0 (0.0%) diarrheic and control children, respectively; STEC and EIEC were not detected. Only EAEC was significantly isolated from diarrheic children compared with controls (p < 0.01, OR = 4.31).Three or more VFs (multivirulent isolates) were found in 52.6% and 50% of EAEC isolated from diarrheic children and controls, respectively. More than 73% (17/23) of EAEC isolates were identified as belonging to phylogenetic group D. Multiple-antibiotic resistance (resistance to three or more drugs) was observed in more than 91% of EAEC.
Multivirulent EAEC is a significant causative agent of pediatric diarrhea in Egypt, with the majority of isolated EAEC belong to phylogenetic group D. Multiple-antibiotic resistance among EAEC has the potential to be a serious public health problem for the country. |
| doi_str_mv | 10.3855/jidc.4077 |
| format | Article |
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Escherichia coli was isolated from stool samples of 62 diarrheic and 43 non-diarrheic (control) Egyptian children. Samples were screened for genes specific for enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), Shiga toxin-producing E. coli (STEC), and enteroinvasive E. coli (EIEC) using polymerase chain reaction (PCR). Diarrheagenic E. coli were grouped phylogenetically using PCR and tested for their susceptibility to antibiotics using the disk diffusion method. Isolates designated as EAEC were examined for eight virulence factors (VFs) using PCR.
EAEC was detected in 19 (30.7%) and 4 (9.3%), EPEC in 2 (3.2%) and 1 (2.3%), and ETEC in 2 (3.2%) and 0 (0.0%) diarrheic and control children, respectively; STEC and EIEC were not detected. Only EAEC was significantly isolated from diarrheic children compared with controls (p < 0.01, OR = 4.31).Three or more VFs (multivirulent isolates) were found in 52.6% and 50% of EAEC isolated from diarrheic children and controls, respectively. More than 73% (17/23) of EAEC isolates were identified as belonging to phylogenetic group D. Multiple-antibiotic resistance (resistance to three or more drugs) was observed in more than 91% of EAEC.
Multivirulent EAEC is a significant causative agent of pediatric diarrhea in Egypt, with the majority of isolated EAEC belong to phylogenetic group D. Multiple-antibiotic resistance among EAEC has the potential to be a serious public health problem for the country.</description><identifier>ISSN: 1972-2680</identifier><identifier>ISSN: 2036-6590</identifier><identifier>EISSN: 1972-2680</identifier><identifier>DOI: 10.3855/jidc.4077</identifier><identifier>PMID: 24820462</identifier><language>eng</language><publisher>Italy: Journal of Infection in Developing Countries</publisher><subject>Anti-Bacterial Agents - pharmacology ; Antibiotics ; Child, Preschool ; Cluster Analysis ; Drug resistance ; E coli ; Egypt - epidemiology ; Escherichia coli - drug effects ; Escherichia coli - genetics ; Escherichia coli - isolation & purification ; Escherichia coli Infections - epidemiology ; Escherichia coli Infections - microbiology ; Female ; Genotype ; Humans ; Infant ; Infant, Newborn ; Male ; Microbial Sensitivity Tests ; Pediatrics ; Phylogenetics ; Phylogeny ; Polymerase Chain Reaction ; Virulence Factors - genetics</subject><ispartof>Journal of infection in developing countries, 2014-05, Vol.8 (5), p.589-596</ispartof><rights>2014. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-db617840dbeab6053453ae4ca0a76edfc94666fedd6cee7a267b2b55e8827ee63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24820462$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ali, Mostafa Mohamed M</creatorcontrib><creatorcontrib>Ahmed, Salwa Fouad</creatorcontrib><creatorcontrib>Klena, John D</creatorcontrib><creatorcontrib>Mohamed, Zienat Kamel</creatorcontrib><creatorcontrib>Moussa, Tarek A A</creatorcontrib><creatorcontrib>Ghenghesh, Khalifa Sifaw</creatorcontrib><title>Enteroaggregative Escherichia coli in diarrheic children in Egypt: molecular characterization and antimicrobial susceptibility</title><title>Journal of infection in developing countries</title><addtitle>J Infect Dev Ctries</addtitle><description>Little information is available regarding the significance of enteroaggregative Escherichia coli (EAEC) in pediatric diarrhea in Egypt.
Escherichia coli was isolated from stool samples of 62 diarrheic and 43 non-diarrheic (control) Egyptian children. Samples were screened for genes specific for enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), Shiga toxin-producing E. coli (STEC), and enteroinvasive E. coli (EIEC) using polymerase chain reaction (PCR). Diarrheagenic E. coli were grouped phylogenetically using PCR and tested for their susceptibility to antibiotics using the disk diffusion method. Isolates designated as EAEC were examined for eight virulence factors (VFs) using PCR.
EAEC was detected in 19 (30.7%) and 4 (9.3%), EPEC in 2 (3.2%) and 1 (2.3%), and ETEC in 2 (3.2%) and 0 (0.0%) diarrheic and control children, respectively; STEC and EIEC were not detected. Only EAEC was significantly isolated from diarrheic children compared with controls (p < 0.01, OR = 4.31).Three or more VFs (multivirulent isolates) were found in 52.6% and 50% of EAEC isolated from diarrheic children and controls, respectively. More than 73% (17/23) of EAEC isolates were identified as belonging to phylogenetic group D. Multiple-antibiotic resistance (resistance to three or more drugs) was observed in more than 91% of EAEC.
Multivirulent EAEC is a significant causative agent of pediatric diarrhea in Egypt, with the majority of isolated EAEC belong to phylogenetic group D. Multiple-antibiotic resistance among EAEC has the potential to be a serious public health problem for the country.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibiotics</subject><subject>Child, Preschool</subject><subject>Cluster Analysis</subject><subject>Drug resistance</subject><subject>E coli</subject><subject>Egypt - epidemiology</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - isolation & purification</subject><subject>Escherichia coli Infections - epidemiology</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Female</subject><subject>Genotype</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Male</subject><subject>Microbial Sensitivity Tests</subject><subject>Pediatrics</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>Virulence Factors - genetics</subject><issn>1972-2680</issn><issn>2036-6590</issn><issn>1972-2680</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdkctLw0AQxhdRfFQP_gMS8KKH6mazj9SbSH2A4EXPYbI7badskrqbCPXg3-4WH4iHYYaZHx_f8DF2nPOLolTqcknOXkhuzBbbzydGjIUu-fafeY8dxLjkXE0Kle-yPSFLwaUW--xj2vYYOpjPA86hpzfMptEuMJBdEGS285RRmzmCEBZINktr7wK2m-10vl71V1nTebSDh5COEMAmQXpPWl2bQetS9dSQDV1N4LM4RIurnmry1K8P2c4MfMSj7z5iL7fT55v78ePT3cPN9ePYFmbSj12tc1NK7mqEWnNVSFUASgscjEY3sxOptZ6hc9oiGhDa1KJWCstSGERdjNjZl-4qdK8Dxr5qKPnwHlrshljlSshC5qXhCT39hy67IbTJXSWU5sKIXJpEnX9R6a8YA86qVaAGwrrKebUJpdqEUm1CSezJt-JQN-h-yZ8Uik-a84sM</recordid><startdate>20140514</startdate><enddate>20140514</enddate><creator>Ali, Mostafa Mohamed M</creator><creator>Ahmed, Salwa Fouad</creator><creator>Klena, John D</creator><creator>Mohamed, Zienat Kamel</creator><creator>Moussa, Tarek A A</creator><creator>Ghenghesh, Khalifa Sifaw</creator><general>Journal of Infection in Developing Countries</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8C1</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20140514</creationdate><title>Enteroaggregative Escherichia coli in diarrheic children in Egypt: molecular characterization and antimicrobial susceptibility</title><author>Ali, Mostafa Mohamed M ; Ahmed, Salwa Fouad ; Klena, John D ; Mohamed, Zienat Kamel ; Moussa, Tarek A A ; Ghenghesh, Khalifa Sifaw</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-db617840dbeab6053453ae4ca0a76edfc94666fedd6cee7a267b2b55e8827ee63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibiotics</topic><topic>Child, Preschool</topic><topic>Cluster Analysis</topic><topic>Drug resistance</topic><topic>E coli</topic><topic>Egypt - epidemiology</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - isolation & purification</topic><topic>Escherichia coli Infections - epidemiology</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Female</topic><topic>Genotype</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Male</topic><topic>Microbial Sensitivity Tests</topic><topic>Pediatrics</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>Virulence Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ali, Mostafa Mohamed M</creatorcontrib><creatorcontrib>Ahmed, Salwa Fouad</creatorcontrib><creatorcontrib>Klena, John D</creatorcontrib><creatorcontrib>Mohamed, Zienat Kamel</creatorcontrib><creatorcontrib>Moussa, Tarek A A</creatorcontrib><creatorcontrib>Ghenghesh, Khalifa Sifaw</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Public Health Database</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of infection in developing countries</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ali, Mostafa Mohamed M</au><au>Ahmed, Salwa Fouad</au><au>Klena, John D</au><au>Mohamed, Zienat Kamel</au><au>Moussa, Tarek A A</au><au>Ghenghesh, Khalifa Sifaw</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enteroaggregative Escherichia coli in diarrheic children in Egypt: molecular characterization and antimicrobial susceptibility</atitle><jtitle>Journal of infection in developing countries</jtitle><addtitle>J Infect Dev Ctries</addtitle><date>2014-05-14</date><risdate>2014</risdate><volume>8</volume><issue>5</issue><spage>589</spage><epage>596</epage><pages>589-596</pages><issn>1972-2680</issn><issn>2036-6590</issn><eissn>1972-2680</eissn><abstract>Little information is available regarding the significance of enteroaggregative Escherichia coli (EAEC) in pediatric diarrhea in Egypt.
Escherichia coli was isolated from stool samples of 62 diarrheic and 43 non-diarrheic (control) Egyptian children. Samples were screened for genes specific for enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), Shiga toxin-producing E. coli (STEC), and enteroinvasive E. coli (EIEC) using polymerase chain reaction (PCR). Diarrheagenic E. coli were grouped phylogenetically using PCR and tested for their susceptibility to antibiotics using the disk diffusion method. Isolates designated as EAEC were examined for eight virulence factors (VFs) using PCR.
EAEC was detected in 19 (30.7%) and 4 (9.3%), EPEC in 2 (3.2%) and 1 (2.3%), and ETEC in 2 (3.2%) and 0 (0.0%) diarrheic and control children, respectively; STEC and EIEC were not detected. Only EAEC was significantly isolated from diarrheic children compared with controls (p < 0.01, OR = 4.31).Three or more VFs (multivirulent isolates) were found in 52.6% and 50% of EAEC isolated from diarrheic children and controls, respectively. More than 73% (17/23) of EAEC isolates were identified as belonging to phylogenetic group D. Multiple-antibiotic resistance (resistance to three or more drugs) was observed in more than 91% of EAEC.
Multivirulent EAEC is a significant causative agent of pediatric diarrhea in Egypt, with the majority of isolated EAEC belong to phylogenetic group D. Multiple-antibiotic resistance among EAEC has the potential to be a serious public health problem for the country.</abstract><cop>Italy</cop><pub>Journal of Infection in Developing Countries</pub><pmid>24820462</pmid><doi>10.3855/jidc.4077</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anti-Bacterial Agents - pharmacology Antibiotics Child, Preschool Cluster Analysis Drug resistance E coli Egypt - epidemiology Escherichia coli - drug effects Escherichia coli - genetics Escherichia coli - isolation & purification Escherichia coli Infections - epidemiology Escherichia coli Infections - microbiology Female Genotype Humans Infant Infant, Newborn Male Microbial Sensitivity Tests Pediatrics Phylogenetics Phylogeny Polymerase Chain Reaction Virulence Factors - genetics |
| title | Enteroaggregative Escherichia coli in diarrheic children in Egypt: molecular characterization and antimicrobial susceptibility |
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